MX352229B - Metodo de produccion de polipeptido recombinante. - Google Patents

Metodo de produccion de polipeptido recombinante.

Info

Publication number
MX352229B
MX352229B MX2013011355A MX2013011355A MX352229B MX 352229 B MX352229 B MX 352229B MX 2013011355 A MX2013011355 A MX 2013011355A MX 2013011355 A MX2013011355 A MX 2013011355A MX 352229 B MX352229 B MX 352229B
Authority
MX
Mexico
Prior art keywords
production method
recombinant polypeptide
polypeptide production
nfkbia
producing
Prior art date
Application number
MX2013011355A
Other languages
English (en)
Other versions
MX2013011355A (es
Inventor
Hisahiro Tabuchi
Tomoya Sugiyama
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of MX2013011355A publication Critical patent/MX2013011355A/es
Publication of MX352229B publication Critical patent/MX352229B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/303—Liver or Pancreas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La presente invención se refiere a un método capaz de producir una proteína de alto nivel usando una célula animal cultivada, que comprende una célula que expresa APES (secuencia mejoradora de producción de anticuerpos) y en la cual se ha introducido un ADN que codifica un polipéptido deseado, lo que produce el polipéptido deseado; APES contiene una secuencia de nucleótidos relacionada con NfkBia y tiene una función de disminuir la expresión intracelular de NfkBia.
MX2013011355A 2011-04-01 2012-03-30 Metodo de produccion de polipeptido recombinante. MX352229B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2011082002 2011-04-01
PCT/JP2012/058577 WO2012137683A1 (ja) 2011-04-01 2012-03-30 組換えポリペプチドの製造方法

Publications (2)

Publication Number Publication Date
MX2013011355A MX2013011355A (es) 2013-12-16
MX352229B true MX352229B (es) 2017-11-15

Family

ID=46969084

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013011355A MX352229B (es) 2011-04-01 2012-03-30 Metodo de produccion de polipeptido recombinante.

Country Status (15)

Country Link
US (2) US11028149B2 (es)
EP (1) EP2695947B1 (es)
JP (3) JP6182066B2 (es)
KR (1) KR101958033B1 (es)
CN (3) CN106995831A (es)
AU (2) AU2012239382B2 (es)
BR (1) BR112013024781B1 (es)
CA (1) CA2830013C (es)
DK (1) DK2695947T3 (es)
ES (1) ES2620306T3 (es)
MX (1) MX352229B (es)
RU (1) RU2628310C2 (es)
SG (1) SG194014A1 (es)
TW (2) TWI647309B (es)
WO (1) WO2012137683A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1601776T1 (sl) * 2003-03-11 2008-10-31 Serono Lab Ekspresijski vektorji, ki vsebujejo mcmv ie2 promotor
JP6463634B2 (ja) * 2012-10-10 2019-02-06 中外製薬株式会社 改変宿主細胞の樹立方法
US9647919B1 (en) * 2014-12-04 2017-05-09 Amazon Technologies Automated determination of maximum service throughput
CN107287178B (zh) * 2016-04-12 2019-10-29 中国科学院微生物研究所 Csad蛋白及其编码基因在抗流感病毒中的应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9222931D0 (en) 1992-11-02 1992-12-16 Sandoz Ltd Organic compounds
TW402639B (en) 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
AU2002213207A1 (en) 2000-10-11 2002-04-22 Digital Gene Technologies, Inc. Gene expression modulated in ileitis
CA2388761A1 (en) * 2001-06-18 2002-12-18 Pfizer Inc. Wound healing biomarkers
EP1784501B1 (en) * 2004-05-14 2015-11-18 Rosetta Genomics Ltd VIRAL AND VIRUS ASSOCIATED MicroRNAS AND USES THEREOF
UA94019C2 (ru) 2004-07-09 2011-04-11 Чугаи Сейяку Кабусики Кайся Антитело, которое специфически связывается с глипиканом 3 (gpc3)
JP2008537940A (ja) 2005-03-29 2008-10-02 ザ・トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルバニア 新しい毛包の形成、はげ頭症の治療、及び除毛をするための方法
JP4977691B2 (ja) 2006-04-13 2012-07-18 中外製薬株式会社 タウリントランスポーター遺伝子
PL2041177T3 (pl) * 2006-06-02 2012-09-28 Regeneron Pharma Przeciwciała o wysokim powinowactwie przeciw ludzkiemu receptorowi IL 6
JP5635260B2 (ja) * 2007-03-15 2014-12-03 中外製薬株式会社 ポリペプチドの製造方法
US8062864B2 (en) * 2007-05-21 2011-11-22 Alderbio Holdings Llc Nucleic acids encoding antibodies to IL-6, and recombinant production of anti-IL-6 antibodies
JP5337033B2 (ja) 2007-08-07 2013-11-06 中外製薬株式会社 異種タンパク質の製造方法
BRPI0818039A2 (pt) * 2007-10-15 2014-10-14 Chugai Pharmaceutical Co Ltd Método para produzir uma célula capaz de produção de alto rendimento de heteroproteínas.
DK2213746T3 (en) 2007-10-24 2015-08-03 Chugai Pharmaceutical Co Ltd CELL FOR USE IN THE PREPARATION OF exogenous protein, AND MANUFACTURING METHOD THAT USE CELL
AU2008348270A1 (en) * 2007-12-21 2009-07-30 Danisco Us Inc. Enhanced protein production in bacillus

Also Published As

Publication number Publication date
CN108546295A (zh) 2018-09-18
JP6182066B2 (ja) 2017-08-16
BR112013024781A2 (pt) 2018-06-05
US20210246193A1 (en) 2021-08-12
CA2830013A1 (en) 2012-10-11
US11028149B2 (en) 2021-06-08
TW201726729A (zh) 2017-08-01
TWI647309B (zh) 2019-01-11
US11905325B2 (en) 2024-02-20
WO2012137683A1 (ja) 2012-10-11
US20140030758A1 (en) 2014-01-30
MX2013011355A (es) 2013-12-16
CN108546295B (zh) 2022-08-09
KR101958033B1 (ko) 2019-03-13
JP2017074065A (ja) 2017-04-20
JP6626160B2 (ja) 2019-12-25
BR112013024781B1 (pt) 2021-09-08
AU2016201744B2 (en) 2017-09-28
AU2012239382B2 (en) 2016-04-21
KR20140015506A (ko) 2014-02-06
CN103459608B (zh) 2018-05-01
JPWO2012137683A1 (ja) 2014-07-28
ES2620306T3 (es) 2017-06-28
DK2695947T3 (en) 2017-05-08
RU2013148804A (ru) 2015-05-10
SG194014A1 (en) 2013-11-29
EP2695947A4 (en) 2014-10-29
CN106995831A (zh) 2017-08-01
JP2018161146A (ja) 2018-10-18
CA2830013C (en) 2020-01-07
JP6444968B2 (ja) 2018-12-26
RU2628310C2 (ru) 2017-08-15
EP2695947B1 (en) 2017-03-01
TW201303019A (zh) 2013-01-16
EP2695947A1 (en) 2014-02-12
TWI674272B (zh) 2019-10-11
AU2012239382A1 (en) 2013-10-17
AU2016201744A1 (en) 2016-04-07
CN103459608A (zh) 2013-12-18

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