BR112012027282A2 - meio de cultura de células aperfeiçoado - Google Patents
meio de cultura de células aperfeiçoadoInfo
- Publication number
- BR112012027282A2 BR112012027282A2 BR112012027282A BR112012027282A BR112012027282A2 BR 112012027282 A2 BR112012027282 A2 BR 112012027282A2 BR 112012027282 A BR112012027282 A BR 112012027282A BR 112012027282 A BR112012027282 A BR 112012027282A BR 112012027282 A2 BR112012027282 A2 BR 112012027282A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell culture
- culture medium
- improved cell
- producing polypeptides
- shift
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0031—Serum-free culture media
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/005—Protein-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
meio de cultura de células aperfeiçoado. a presente invenção refere-se a um meio otimizado para o crescimento de células de mamíferos, bem como a produção de polipeptídeos. o meio de cultura de células é distiguido por uma razão sow de íons de sódio para potássio, e ele refere-se ainda ao método para produzir polipeptídeos usando tais meios de cultura de células. em outro aspecto, o método para produzir polipeptídeos usando pode compreender também um deslocamento de temperatura e/ou um deslocamento de ph para otimizar ainda mais o crescimento e o rendimento do produto.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US32783610P | 2010-04-26 | 2010-04-26 | |
US61/327,836 | 2010-04-26 | ||
PCT/EP2011/056508 WO2011134920A1 (en) | 2010-04-26 | 2011-04-25 | Improved cell culture medium |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112012027282A2 true BR112012027282A2 (pt) | 2015-09-22 |
BR112012027282B1 BR112012027282B1 (pt) | 2020-09-29 |
Family
ID=44074260
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112012027282-9A BR112012027282B1 (pt) | 2010-04-26 | 2011-04-25 | Meio de cultura de células aperfeiçoado e processo para a produção de um polipeptídeo recombinante |
Country Status (17)
Country | Link |
---|---|
US (2) | US9428727B2 (pt) |
EP (1) | EP2563904B1 (pt) |
JP (3) | JP6347949B2 (pt) |
KR (1) | KR101828623B1 (pt) |
CN (1) | CN102858952B (pt) |
AU (1) | AU2011246503B2 (pt) |
BR (1) | BR112012027282B1 (pt) |
CA (1) | CA2797356C (pt) |
DK (1) | DK2563904T3 (pt) |
ES (1) | ES2533074T3 (pt) |
IL (1) | IL222452B (pt) |
MX (1) | MX2012012526A (pt) |
PL (1) | PL2563904T3 (pt) |
PT (1) | PT2563904E (pt) |
RU (1) | RU2644651C2 (pt) |
SG (1) | SG185038A1 (pt) |
WO (1) | WO2011134920A1 (pt) |
Families Citing this family (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
KR20090074040A (ko) | 2006-09-13 | 2009-07-03 | 아보트 러보러터리즈 | 세포 배양의 개선 |
SG10201702922VA (en) | 2008-10-20 | 2017-06-29 | Abbvie Inc | Isolation and purification of antibodies using protein a affinity chromatography |
SG10201702951RA (en) | 2008-10-20 | 2017-06-29 | Abbvie Inc | Viral inactivation during purification of antibodies |
US9428727B2 (en) * | 2010-04-26 | 2016-08-30 | Novartis Ag | Cell culture medium |
MX2012012525A (es) | 2010-04-26 | 2012-11-23 | Novartis Ag | Medio de cultivo mejorado. |
MX345885B (es) * | 2010-12-07 | 2017-02-22 | Hoffmann La Roche | Dispositivo de mezclado de alimentacion y su uso. |
EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
US10493151B2 (en) | 2011-10-18 | 2019-12-03 | Coherus Biosciences, Inc. | Etanercept formulations stabilized with sodium chloride |
JP6113176B2 (ja) | 2011-10-18 | 2017-04-12 | コヒラス・バイオサイエンシズ・インコーポレイテッド | キシリトールによって安定化されたエタネルセプト製剤 |
WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
US9505833B2 (en) | 2012-04-20 | 2016-11-29 | Abbvie Inc. | Human antibodies that bind human TNF-alpha and methods of preparing the same |
US9334319B2 (en) | 2012-04-20 | 2016-05-10 | Abbvie Inc. | Low acidic species compositions |
US20130281355A1 (en) | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
EA029193B1 (ru) | 2012-07-09 | 2018-02-28 | Кохерус Байосайенсис, Инк. | Составы этанерцепта, отличающиеся заметным уменьшением содержания частиц довидимого диапазона |
US9206390B2 (en) | 2012-09-02 | 2015-12-08 | Abbvie, Inc. | Methods to control protein heterogeneity |
US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
IN2015KN00452A (pt) | 2012-09-11 | 2015-07-17 | Coherus Biosciences Inc | |
WO2014159579A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE |
US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
WO2014158231A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | Low acidic species compositions and methods for producing and using the same |
JP2016527911A (ja) | 2013-08-20 | 2016-09-15 | レック・ファーマシューティカルズ・ディー・ディーLek Pharmaceuticals D.D. | ポリペプチドのα−アミド化および/またはC末端アミノ酸開裂を制御するための細胞培養用培地およびプロセス |
EP3052640A2 (en) | 2013-10-04 | 2016-08-10 | AbbVie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
AU2014331776B2 (en) * | 2013-10-11 | 2020-10-22 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
MX362923B (es) * | 2014-01-30 | 2019-02-25 | Coherus Biosciences Inc | Medios de perfusión. |
RU2710726C2 (ru) | 2014-04-29 | 2020-01-10 | Новартис Аг | Новые клетки позвоночных и способы рекомбинантной экспрессии интересующего полипептида |
ES2733298T3 (es) | 2014-07-18 | 2019-11-28 | Sandoz Ag | Cuantificación de TNFR2:Fc plegado erróneamente |
JP6876049B2 (ja) * | 2015-12-17 | 2021-05-26 | ライフ テクノロジーズ コーポレーション | 細胞培養に使用されるペレットおよびその製造方法 |
US20190112572A1 (en) * | 2016-04-05 | 2019-04-18 | Pfizer Inc. | Cell culture process |
EP3601586A1 (en) * | 2017-03-31 | 2020-02-05 | Boehringer Ingelheim International GmbH | Perfusion medium |
CN110462054B (zh) * | 2017-03-31 | 2023-11-10 | 勃林格殷格翰国际公司 | 灌注培养基 |
EP4056678A4 (en) * | 2019-11-05 | 2023-12-06 | Ajinomoto Co., Inc. | METHOD FOR PRODUCING A PROTEIN |
WO2022036275A2 (en) * | 2020-08-14 | 2022-02-17 | Bristol-Myers Squibb Company | Manufacturing process for protein |
UY39484A (es) | 2020-11-02 | 2022-06-30 | Novartis Ag | Inhibidores de interleucina-17 |
Family Cites Families (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5672502A (en) | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
DE3871206D1 (de) | 1987-03-24 | 1992-06-25 | Grace W R & Co | Basisnaehrmedium fuer eine zellkultur. |
US6048728A (en) * | 1988-09-23 | 2000-04-11 | Chiron Corporation | Cell culture medium for enhanced cell growth, culture longevity, and product expression |
US5135866A (en) | 1989-03-03 | 1992-08-04 | W. R. Grace & Co.-Conn. | Very low protein nutrient medium for cell culture |
CA2001550A1 (en) | 1989-03-03 | 1990-09-03 | Aaron H. Heifetz | Very low protein nutrient medium for cell culture |
US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
JPH0789908B2 (ja) | 1991-02-28 | 1995-10-04 | 倉敷紡績株式会社 | 動物細胞培養用無血清培地 |
GB9118664D0 (en) | 1991-08-30 | 1991-10-16 | Celltech Ltd | Cell culture |
US5856179A (en) | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
US5830761A (en) | 1995-06-07 | 1998-11-03 | Genetics Institute, Inc. | Medium and methods for culturing mammalian cho cells |
EP1482031B1 (en) * | 1996-08-30 | 2015-10-28 | Life Technologies Corporation | Serum-free mammalian cell culture medium, and uses thereof |
US5804420A (en) | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
US6406909B1 (en) | 1998-07-10 | 2002-06-18 | Chugai Seiyaku Kabushiki Kaisha | Serum-free medium for culturing animal cells |
US7294481B1 (en) | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
EP1757700A3 (en) | 1999-04-26 | 2009-03-11 | Genentech, Inc. | Cell culture process for glycoproteins |
AU4980200A (en) | 1999-05-27 | 2000-12-18 | Pharmacia & Upjohn Company | Methods and compositions for measuring ion channel conductance |
AT409379B (de) * | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
AU780653B2 (en) | 1999-12-28 | 2005-04-07 | Isotis N.V. | Cell culture medium containing growth factors and L-glutamine |
WO2002066603A2 (en) | 2001-02-15 | 2002-08-29 | Centocor, Inc. | Chemically defined medium for cultured mammalian cells |
US20030087372A1 (en) | 2001-06-13 | 2003-05-08 | Genentech, Inc. | Methods of culturing animal cells and polypeptide production in animal cells |
DK1543106T3 (da) | 2002-07-15 | 2007-08-13 | Immunex Corp | Fremgangsmåder og medier til kontrol af sialylering af proteiner, der dannes af mammaliaceller |
MXPA05006523A (es) | 2002-12-23 | 2005-08-26 | Squibb Bristol Myers Co | Procesos de cultivo de celulas de mamiferos para la produccion de proteinas. |
GB0304799D0 (en) | 2003-03-03 | 2003-04-09 | Glaxosmithkline Biolog Sa | Novel method |
DE10338536A1 (de) | 2003-08-19 | 2005-04-07 | Bircher Reglomat Ag | Verfahren zum Betreiben eines Radarsensors |
TWI384069B (zh) * | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
TWI374935B (en) | 2004-08-27 | 2012-10-21 | Pfizer Ireland Pharmaceuticals | Production of α-abeta |
EP1707634A1 (en) | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
WO2006113629A1 (en) | 2005-04-15 | 2006-10-26 | The Trustees Of Columbia University In The City Of New York | Chemically defined culture media for expansion and differentiation of epidermal cells and uses thereof for in vitro growth of hair follicles |
US20070231895A1 (en) | 2005-11-02 | 2007-10-04 | Lee Gene W | Methods for adapting mammalian cells |
US20070190057A1 (en) * | 2006-01-23 | 2007-08-16 | Jian Wu | Methods for modulating mannose content of recombinant proteins |
ES2538986T3 (es) * | 2006-11-08 | 2015-06-25 | Wyeth Llc | Medios diseñados racionalmente para un cultivo celular |
US9428727B2 (en) * | 2010-04-26 | 2016-08-30 | Novartis Ag | Cell culture medium |
-
2011
- 2011-04-25 US US13/643,998 patent/US9428727B2/en not_active Ceased
- 2011-04-25 SG SG2012079174A patent/SG185038A1/en unknown
- 2011-04-25 PL PL11717240T patent/PL2563904T3/pl unknown
- 2011-04-25 CN CN201180021024.XA patent/CN102858952B/zh active Active
- 2011-04-25 CA CA2797356A patent/CA2797356C/en active Active
- 2011-04-25 BR BR112012027282-9A patent/BR112012027282B1/pt active IP Right Grant
- 2011-04-25 KR KR1020127030709A patent/KR101828623B1/ko active IP Right Grant
- 2011-04-25 PT PT117172403T patent/PT2563904E/pt unknown
- 2011-04-25 AU AU2011246503A patent/AU2011246503B2/en active Active
- 2011-04-25 JP JP2013506612A patent/JP6347949B2/ja active Active
- 2011-04-25 ES ES11717240.3T patent/ES2533074T3/es active Active
- 2011-04-25 EP EP11717240.3A patent/EP2563904B1/en not_active Revoked
- 2011-04-25 MX MX2012012526A patent/MX2012012526A/es active IP Right Grant
- 2011-04-25 WO PCT/EP2011/056508 patent/WO2011134920A1/en active Application Filing
- 2011-04-25 RU RU2012150346A patent/RU2644651C2/ru active
- 2011-04-25 DK DK11717240T patent/DK2563904T3/en active
- 2011-04-25 US US16/114,731 patent/USRE48864E1/en active Active
-
2012
- 2012-10-15 IL IL222452A patent/IL222452B/en active IP Right Grant
-
2016
- 2016-08-03 JP JP2016152747A patent/JP6603628B2/ja active Active
-
2019
- 2019-08-01 JP JP2019142124A patent/JP7034991B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
CA2797356A1 (en) | 2011-11-03 |
RU2018101201A3 (pt) | 2021-05-27 |
USRE48864E1 (en) | 2021-12-28 |
CA2797356C (en) | 2020-12-29 |
AU2011246503B2 (en) | 2013-09-26 |
ES2533074T3 (es) | 2015-04-07 |
CN102858952A (zh) | 2013-01-02 |
US20130122543A1 (en) | 2013-05-16 |
IL222452A0 (en) | 2012-12-31 |
JP2013524825A (ja) | 2013-06-20 |
US9428727B2 (en) | 2016-08-30 |
PL2563904T3 (pl) | 2015-06-30 |
IL222452B (en) | 2019-03-31 |
RU2644651C2 (ru) | 2018-02-13 |
JP7034991B2 (ja) | 2022-03-14 |
BR112012027282B1 (pt) | 2020-09-29 |
AU2011246503A1 (en) | 2012-11-22 |
DK2563904T3 (en) | 2015-04-07 |
EP2563904A1 (en) | 2013-03-06 |
MX2012012526A (es) | 2012-11-23 |
PT2563904E (pt) | 2015-05-12 |
JP6603628B2 (ja) | 2019-11-06 |
JP6347949B2 (ja) | 2018-06-27 |
WO2011134920A1 (en) | 2011-11-03 |
KR20130058699A (ko) | 2013-06-04 |
JP2019216728A (ja) | 2019-12-26 |
EP2563904B1 (en) | 2015-01-21 |
SG185038A1 (en) | 2012-11-29 |
RU2018101201A (ru) | 2019-02-21 |
RU2012150346A (ru) | 2014-06-10 |
KR101828623B1 (ko) | 2018-02-12 |
CN102858952B (zh) | 2015-10-14 |
JP2017012180A (ja) | 2017-01-19 |
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Legal Events
Date | Code | Title | Description |
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B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 25/04/2011, OBSERVADAS AS CONDICOES LEGAIS. |