DK1543106T3 - Fremgangsmåder og medier til kontrol af sialylering af proteiner, der dannes af mammaliaceller - Google Patents

Fremgangsmåder og medier til kontrol af sialylering af proteiner, der dannes af mammaliaceller

Info

Publication number
DK1543106T3
DK1543106T3 DK03764511T DK03764511T DK1543106T3 DK 1543106 T3 DK1543106 T3 DK 1543106T3 DK 03764511 T DK03764511 T DK 03764511T DK 03764511 T DK03764511 T DK 03764511T DK 1543106 T3 DK1543106 T3 DK 1543106T3
Authority
DK
Denmark
Prior art keywords
methods
cells
mammalian cells
media
proteins produced
Prior art date
Application number
DK03764511T
Other languages
English (en)
Inventor
Brian D Follstad
Original Assignee
Immunex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunex Corp filed Critical Immunex Corp
Application granted granted Critical
Publication of DK1543106T3 publication Critical patent/DK1543106T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
DK03764511T 2002-07-15 2003-07-14 Fremgangsmåder og medier til kontrol af sialylering af proteiner, der dannes af mammaliaceller DK1543106T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39622102P 2002-07-15 2002-07-15
PCT/US2003/021733 WO2004008100A2 (en) 2002-07-15 2003-07-14 Methods and media for controlling sialylation of proteins produced by mammalian cells

Publications (1)

Publication Number Publication Date
DK1543106T3 true DK1543106T3 (da) 2007-08-13

Family

ID=30115987

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03764511T DK1543106T3 (da) 2002-07-15 2003-07-14 Fremgangsmåder og medier til kontrol af sialylering af proteiner, der dannes af mammaliaceller

Country Status (15)

Country Link
US (1) US7645609B2 (da)
EP (1) EP1543106B1 (da)
JP (1) JP4510938B2 (da)
AT (1) ATE359358T1 (da)
AU (1) AU2003249055B2 (da)
CA (1) CA2492267C (da)
CY (1) CY1107689T1 (da)
DE (1) DE60313188T2 (da)
DK (1) DK1543106T3 (da)
ES (1) ES2285188T3 (da)
MX (1) MXPA05000552A (da)
PL (1) PL376381A1 (da)
PT (1) PT1543106E (da)
SI (1) SI1543106T1 (da)
WO (1) WO2004008100A2 (da)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4496086B2 (ja) 2002-12-23 2010-07-07 ブリストル−マイヤーズ スクイブ カンパニー タンパク質製造のための哺乳類細胞培養方法を用いる生成物品質の増大
WO2006044790A2 (en) * 2004-10-15 2006-04-27 Zymequest, Inc. Compositions and methods for prolonging survival of platelets
ES2376777T3 (es) * 2004-09-07 2012-03-16 Velico Medical, Inc. Aparato para prolongar la supervivencia de plaquetas
CA2614039C (en) 2005-07-22 2015-10-13 Five Prime Therapeutics, Inc. Compositions and methods of treating disease with fgfr fusion proteins
WO2007024743A2 (en) * 2005-08-19 2007-03-01 Centocor, Inc. Proteolysis resistant antibody preparations
EP1954304A4 (en) * 2005-10-14 2010-03-10 Zymequest Inc COMPOSITIONS AND METHOD FOR EXTENDING THE LIFE OF PLATES
AR058568A1 (es) 2005-12-20 2008-02-13 Bristol Myers Squibb Co Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo
TWI398520B (zh) * 2005-12-20 2013-06-11 必治妥美雅史谷比公司 組合物及製造組合物之方法
US8129145B2 (en) 2006-07-13 2012-03-06 Wyeth Llc Production of glycoproteins
MX346523B (es) 2006-09-13 2017-03-23 Abbvie Inc Mejoras de cultivos celulares.
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
EP2120998B1 (en) 2006-11-28 2013-08-07 HanAll Biopharma Co., Ltd. Modified erythropoietin polypeptides and uses thereof for treatment
WO2008120570A1 (ja) * 2007-03-19 2008-10-09 National University Corporation Okayama University タンパク質産生用およびウイルス増殖用の培地
TW200902708A (en) 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
US8338569B2 (en) 2008-08-04 2012-12-25 Five Prime Therapeutics, Inc. FGFR extracellular domain acidic region muteins
SG195577A1 (en) 2008-10-20 2013-12-30 Abbott Lab Viral inactivation during purification of antibodies
CN105111309A (zh) 2008-10-20 2015-12-02 Abbvie公司 使用a蛋白亲和层析分离和纯化抗体
EP2233502A1 (en) * 2009-03-27 2010-09-29 Deutsches Rheuma-Forschungszentrum Berlin Sialylated antigen-specific antibodies for treatment or prophylaxis of unwanted inflammatory immune reactions and methods of producing them
US8445445B2 (en) 2009-09-15 2013-05-21 Five Prime Therapeutics, Inc. Method of promoting hair growth using FGFR4 extracellular domains
AU2010319327B2 (en) 2009-11-13 2015-08-13 Five Prime Therapeutics, Inc. Use of FGFR1 extra cellular domain proteins to treat cancers characterized by ligand-dependent activating mutations in FGFR2
NZ600278A (en) 2009-11-13 2014-04-30 Grifols Therapeutics Inc Von willebrand factor (vwf)-containing preparations, and methods, kits, and uses related thereto
EP2325296A1 (en) 2009-11-20 2011-05-25 LEK Pharmaceuticals d.d. Production of glycoproteins with low N-glycolylneuraminic acid (Neu5Gc) content
US8685931B2 (en) 2009-12-17 2014-04-01 Five Prime Therapeutics, Inc. Hair growth methods using FGFR3 extracellular domains
JP6347949B2 (ja) 2010-04-26 2018-06-27 ノバルティス アーゲー 改良型細胞培養培地
US8481038B2 (en) 2010-11-15 2013-07-09 Five Prime Therapeutics, Inc. Treatment of cancer with elevated dosages of soluble FGFR1 fusion proteins
US8951972B2 (en) 2010-12-09 2015-02-10 Five Prime Therapeutics, Inc. FGFR1 extracellular domain combination therapies for lung cancer
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
BR112014000352A2 (pt) * 2011-07-08 2017-02-14 Merck Sharp & Dohe Corp método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada
WO2013074492A1 (en) 2011-11-14 2013-05-23 Five Prime Therapeutics, Inc. Methods of treating cancer
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
AU2013309506A1 (en) 2012-09-02 2015-03-12 Abbvie Inc. Methods to control protein heterogeneity
US20140106405A1 (en) * 2012-10-15 2014-04-17 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
WO2014143205A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
US9499614B2 (en) * 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
TWI771890B (zh) 2013-03-14 2022-07-21 美商安美基公司 用於增加重組蛋白質之甘露糖含量之方法
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US8956830B2 (en) 2013-03-14 2015-02-17 Momenta Pharmaceuticals, Inc. Methods of cell culture
JP6689189B2 (ja) 2013-03-26 2020-04-28 コヒラス・バイオサイエンシズ・インコーポレイテッド タンパク質産生方法
US9481901B2 (en) * 2013-05-30 2016-11-01 Amgen Inc. Methods for increasing mannose content of recombinant proteins
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
CN106102749B (zh) * 2014-03-05 2019-07-12 奥特吉尼克斯制药公司 唾液酸化糖蛋白组合物及其用途
JP6652334B2 (ja) * 2014-05-31 2020-02-19 Jcrファーマ株式会社 ウリジンとn−アセチル−d−マンノサミンとを含有する培地
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
BR112018010160A8 (pt) 2015-11-19 2019-02-26 Shire Human Genetic Therapies inibidor da c1 esterase humana recombinante e usos do mesmo
US20220091129A1 (en) * 2018-12-21 2022-03-24 Jingjie Ptm Biolab (Hangzhou) Co., Inc Reagents and methods for detecting protein lysine lactylation
JP2024503239A (ja) 2020-12-22 2024-01-25 アムジェン インコーポレイテッド 細胞培養法
JP2024509543A (ja) 2021-03-03 2024-03-04 フォーマイコン アーゲー ACE2 Fc融合タンパク質の製剤
WO2023094507A1 (en) 2021-11-24 2023-06-01 Formycon Ag Improved ace2 fusion proteins
CN114317446B (zh) * 2021-12-30 2023-12-05 广州康盛生物科技股份有限公司 一种无血清培养基用添加剂及其应用
EP4331571A1 (en) 2022-09-02 2024-03-06 Formycon AG Formulations of ace2-igm fusion proteins

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5672502A (en) * 1985-06-28 1997-09-30 Celltech Therapeutics Limited Animal cell culture
US5047335A (en) * 1988-12-21 1991-09-10 The Regents Of The University Of Calif. Process for controlling intracellular glycosylation of proteins
JP3375140B2 (ja) 1991-03-08 2003-02-10 森永製菓株式会社 モノクローナル抗体生産用培地の作製法及びそのキット
DE69311873T2 (de) * 1992-05-01 1998-02-12 Teijin Ltd Fed-batch-Verfahren für Proteine sekretierende Zellen
JPH06292592A (ja) * 1993-02-09 1994-10-21 Snow Brand Milk Prod Co Ltd 糖蛋白質の生産方法
US5858783A (en) * 1993-05-25 1999-01-12 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Production of normal mammalian organ culture using a medium containing mem-alpha, leibovitz L-15, glucose galactose fructose
ES2264181T3 (es) * 1997-10-01 2006-12-16 Dsm Ip Assets B.V. Procedimiento para la produccion de proteinas.
WO1999028455A1 (de) * 1997-12-03 1999-06-10 Roche Diagnostics Gmbh Verfahren zur herstellung von polypeptiden mit geeigneter glykosilierung
WO1999028346A1 (de) 1997-12-03 1999-06-10 Roche Diagnostics Gmbh Erythropoietin mit hoher spezifischer aktivität
AU5339699A (en) * 1998-08-06 2000-02-28 Johns Hopkins University School Of Medicine, The Compounds for altering cell surface sialic acids and methods of use therefor
DE19852729A1 (de) 1998-11-16 2000-05-18 Werner Reutter Rekombinante Glycoproteine, Verfahren zu ihrer Herstellung, sie enthaltende Arzneimittel und ihre Verwendung
US6949372B2 (en) * 1999-03-02 2005-09-27 The Johns Hopkins University Engineering intracellular sialylation pathways
US6261805B1 (en) * 1999-07-15 2001-07-17 Boyce Thompson Institute For Plant Research, Inc. Sialyiation of N-linked glycoproteins in the baculovirus expression vector system
EP1298217A4 (en) * 2000-07-05 2004-09-08 Mitsubishi Pharma Corp GLYCOPROTEIN PRODUCTION PROCESS
US20040214228A9 (en) * 2001-09-14 2004-10-28 Ganesh Venkataraman Methods of evaluating glycomolecules for enhanced activities
JP4281797B2 (ja) 2006-12-21 2009-06-17 三菱自動車工業株式会社 車両の車体構造

Also Published As

Publication number Publication date
CA2492267C (en) 2013-09-10
ATE359358T1 (de) 2007-05-15
AU2003249055A1 (en) 2004-02-02
US20040115768A1 (en) 2004-06-17
EP1543106A2 (en) 2005-06-22
EP1543106A4 (en) 2005-08-10
JP2005532813A (ja) 2005-11-04
SI1543106T1 (sl) 2007-08-31
JP4510938B2 (ja) 2010-07-28
AU2003249055B2 (en) 2008-06-26
CA2492267A1 (en) 2004-01-22
CY1107689T1 (el) 2013-04-18
MXPA05000552A (es) 2005-04-28
WO2004008100A2 (en) 2004-01-22
WO2004008100A3 (en) 2004-03-25
DE60313188D1 (de) 2007-05-24
EP1543106B1 (en) 2007-04-11
PT1543106E (pt) 2007-05-31
PL376381A1 (en) 2005-12-27
ES2285188T3 (es) 2007-11-16
US7645609B2 (en) 2010-01-12
DE60313188T2 (de) 2007-12-20

Similar Documents

Publication Publication Date Title
DK1543106T3 (da) Fremgangsmåder og medier til kontrol af sialylering af proteiner, der dannes af mammaliaceller
EP1360314A4 (en) CHEMICALLY DEFINED MEDIUM FOR CULTIVATED MAMMALIAN CELLS
BR0311413A (pt) Desenvolvimento e transdiferenciação de células acinares humanas
DK1918364T3 (da) Fremgangsmåde til rekloning af produktionsceller
GB2429211A (en) Feeder independent extended culture of embryonic stem cells
DK1863920T3 (da) Fremgangsmåde til forbedret isolering af rekombinant producerede proteiner
ATE400651T1 (de) Verwendung von grünem fluoreszenzprotein
ATE421994T1 (de) Genexpressionsmethode
WO2004087896A3 (en) Hepatocyte differentiation of stem cells
UA92157C2 (ru) Способ продуцирования гормона роста
ATE414162T1 (de) Verfahren und materialien zur herstellung von glucosamin
DK1720979T3 (da) Anvendelse af et serumfrit celledyrkningsmedium til fremstilling af IL-18BP I pattedyrceller
DE60138127D1 (de) Stoffwechseländerungen in gärungen zur produktion rekombinanter proteine
DE60041821D1 (de) Pluripotent embryonale stammzellen-ähnliche zellen, zusammensetzungen und ihre vervendungen
ATE466073T1 (de) Basalmedium zur kultivierung von es-zellen
DK1321477T3 (da) Fremgangsmåde til frembringelsen af genetisk modificerede vertebrate precursor-lymfocytter og anvendelse deraf til fremstillingen af heterologe bindende proteiner
MX2007012954A (es) Sistemas de expresion en mamiferos.
ATE330003T1 (de) Rekombinanter stabiler zellklon, seine herstellung und verwendung
ATE454444T1 (de) Verfahren zur herstellung rekombinanter proteine
WO2002002591A3 (en) Peptides for use in culture media
DE50310917D1 (de) Feedback-resistente Homoserin-Transsuccinylasen
DK0839211T3 (da) Fremgangsmåde til fremstilling af riboflavin ved hjælp af mikroorganismer med ændret isocitratlyaseaktivitet
WO2005054447A3 (en) Müller stem cells
ATE378397T1 (de) Verfahren zum kultivieren von mikroorganismen unter reduzierenden bedingungen mittels einer gasströmung
ATE465265T1 (de) Regulierbare genexpression in säugerzellen und säugern