WO2008120570A1 - タンパク質産生用およびウイルス増殖用の培地 - Google Patents

タンパク質産生用およびウイルス増殖用の培地 Download PDF

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Publication number
WO2008120570A1
WO2008120570A1 PCT/JP2008/054918 JP2008054918W WO2008120570A1 WO 2008120570 A1 WO2008120570 A1 WO 2008120570A1 JP 2008054918 W JP2008054918 W JP 2008054918W WO 2008120570 A1 WO2008120570 A1 WO 2008120570A1
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WIPO (PCT)
Prior art keywords
protein
virus
culture medium
production
cell
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PCT/JP2008/054918
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English (en)
French (fr)
Inventor
Hikaru Namba
Yujiro Arao
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National University Corporation Okayama University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by National University Corporation Okayama University filed Critical National University Corporation Okayama University
Priority to JP2009507454A priority Critical patent/JP5634710B2/ja
Publication of WO2008120570A1 publication Critical patent/WO2008120570A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16651—Methods of production or purification of viral material
    • C12N2710/16652—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

本発明は、動物胞を用いて所望のタンパク質の産生および／または所望のウイルスの増殖させるための細胞培養用培地を提供することを課題とする。さらに、本発明はこれらの培地を用いたタンパク質の産生方法およびウイルスの増殖促進方法を提供することを課題とする。メリビオースなどの糖類を含む培地を用いて動物細胞を培養すると、細胞内に組み込まれた発現プロモーターが活性化されてタンパク質の産生が促進し、または細胞に感染しているウイルスが顕著に増殖する。
PCT/JP2008/054918 2007-03-19 2008-03-17 タンパク質産生用およびウイルス増殖用の培地 WO2008120570A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009507454A JP5634710B2 (ja) 2007-03-19 2008-03-17 タンパク質産生用およびウイルス増殖用の培地

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-071390 2007-03-19
JP2007071390 2007-03-19
JP2007238422 2007-09-13
JP2007-238422 2007-09-13

Publications (1)

Publication Number Publication Date
WO2008120570A1 true WO2008120570A1 (ja) 2008-10-09

Family

ID=39808152

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/054918 WO2008120570A1 (ja) 2007-03-19 2008-03-17 タンパク質産生用およびウイルス増殖用の培地

Country Status (2)

Country Link
JP (2) JP5634710B2 (ja)
WO (1) WO2008120570A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160185848A1 (en) * 2014-07-09 2016-06-30 Abbvie Inc. Methods for modulating the glycosylation profile of recombinant proteins using sugars
US9708400B2 (en) 2012-04-20 2017-07-18 Abbvie, Inc. Methods to modulate lysine variant distribution
US9957318B2 (en) 2012-04-20 2018-05-01 Abbvie Inc. Protein purification methods to reduce acidic species

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50116683A (ja) * 1974-02-20 1975-09-12
JPS57105186A (en) * 1976-11-16 1982-06-30 Univ Alabama New maltase enzyme produced from new yeast strain and preparation thereof
JPH02200177A (ja) * 1989-01-31 1990-08-08 Nippon Kayaku Co Ltd 動物細胞培養用組成物、培養方法および生理活性物質の製造法
JPH0595783A (ja) * 1991-10-04 1993-04-20 Nitsusui Seiyaku Kk 浮遊細胞用無血清合成培地
JPH05505312A (ja) * 1990-12-28 1993-08-12 ビヨメリウー サルモネラ属の細菌を検出するための細菌学的分析方法及び培地
JPH0662833A (ja) * 1992-08-19 1994-03-08 Nitsusui Seiyaku Kk サルモネラ分離用培地
JPH11113484A (ja) * 1997-10-16 1999-04-27 Snow Brand Rolly Co Ltd ビフィズス菌の生残性改善方法
JPH11127890A (ja) * 1997-10-31 1999-05-18 Kazuo Shimada 糖蛋白質の生産方法
JP2000139451A (ja) * 1998-11-04 2000-05-23 Kao Corp 腸内酪酸菌生育促進剤
JP2002253245A (ja) * 2001-02-28 2002-09-10 Toyota Central Res & Dev Lab Inc 融合タンパク質
JP2004283176A (ja) * 1990-12-07 2004-10-14 Univ Of Florida Research Foundation Inc 染色体に組込まれた異種遺伝子を高度に発現する組換え細胞
JP2005532813A (ja) * 2002-07-15 2005-11-04 イミュネックス・コーポレーション 哺乳動物細胞により産生されるタンパク質のシアル酸付加を調節する方法および培地
JP2006504412A (ja) * 2002-08-23 2006-02-09 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 発酵による生物的生産のためのデンプン生成物の利用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4532493B2 (ja) * 2003-09-18 2010-08-25 レイベン バイオテクノロジーズ，インコーポレイティド 細胞培養培地

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50116683A (ja) * 1974-02-20 1975-09-12
JPS57105186A (en) * 1976-11-16 1982-06-30 Univ Alabama New maltase enzyme produced from new yeast strain and preparation thereof
JPH02200177A (ja) * 1989-01-31 1990-08-08 Nippon Kayaku Co Ltd 動物細胞培養用組成物、培養方法および生理活性物質の製造法
JP2004283176A (ja) * 1990-12-07 2004-10-14 Univ Of Florida Research Foundation Inc 染色体に組込まれた異種遺伝子を高度に発現する組換え細胞
JPH05505312A (ja) * 1990-12-28 1993-08-12 ビヨメリウー サルモネラ属の細菌を検出するための細菌学的分析方法及び培地
JPH0595783A (ja) * 1991-10-04 1993-04-20 Nitsusui Seiyaku Kk 浮遊細胞用無血清合成培地
JPH0662833A (ja) * 1992-08-19 1994-03-08 Nitsusui Seiyaku Kk サルモネラ分離用培地
JPH11113484A (ja) * 1997-10-16 1999-04-27 Snow Brand Rolly Co Ltd ビフィズス菌の生残性改善方法
JPH11127890A (ja) * 1997-10-31 1999-05-18 Kazuo Shimada 糖蛋白質の生産方法
JP2000139451A (ja) * 1998-11-04 2000-05-23 Kao Corp 腸内酪酸菌生育促進剤
JP2002253245A (ja) * 2001-02-28 2002-09-10 Toyota Central Res & Dev Lab Inc 融合タンパク質
JP2005532813A (ja) * 2002-07-15 2005-11-04 イミュネックス・コーポレーション 哺乳動物細胞により産生されるタンパク質のシアル酸付加を調節する方法および培地
JP2006504412A (ja) * 2002-08-23 2006-02-09 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 発酵による生物的生産のためのデンプン生成物の利用

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BAILEY J.M. ET AL.: "The carbohydrate nutrition and metabolism of a strain of mammalian cells (MB III strain of mouse lymphoblasts) growing in vitro", J. BIOL. CHEM., vol. 234, no. 5, 1959, pages 1042 - 1047 *
DEMETRAKOPOULOS G.E. ET AL.: "Growth of chick and mammalian cells on D-xylose effects on sugar transport", EXPERIMENTAL CELL RESEARCH, vol. 106, no. 1, 1977, pages 167 - 173, XP024859240, DOI: doi:10.1016/0014-4827(77)90253-1 *
PETCH D. ET AL.: "The effect of alternative carbohydrate on the growth and antibody production of a murine hybridoma", APPL. BIOCHEM. BIOTECHNOL., vol. 59, no. 1, 1996, pages 93 - 104, XP035176311, DOI: doi:10.1007/BF02787861 *
SCANNELL J. ET AL.: "The regulation of carbohydrate metabolism in animal cells: Isolation of starch- and maltose-utilizing variants", BIOSCIENCE REPORTS, vol. 2, no. 2, 1982, pages 99 - 106 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9708400B2 (en) 2012-04-20 2017-07-18 Abbvie, Inc. Methods to modulate lysine variant distribution
US9957318B2 (en) 2012-04-20 2018-05-01 Abbvie Inc. Protein purification methods to reduce acidic species
US20160185848A1 (en) * 2014-07-09 2016-06-30 Abbvie Inc. Methods for modulating the glycosylation profile of recombinant proteins using sugars

Also Published As

Publication number Publication date
JP5748808B2 (ja) 2015-07-15
JP2013240353A (ja) 2013-12-05
JPWO2008120570A1 (ja) 2010-07-15
JP5634710B2 (ja) 2014-12-03

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