BR112014000352A2 - método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada - Google Patents

método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada

Info

Publication number
BR112014000352A2
BR112014000352A2 BR112014000352A BR112014000352A BR112014000352A2 BR 112014000352 A2 BR112014000352 A2 BR 112014000352A2 BR 112014000352 A BR112014000352 A BR 112014000352A BR 112014000352 A BR112014000352 A BR 112014000352A BR 112014000352 A2 BR112014000352 A2 BR 112014000352A2
Authority
BR
Brazil
Prior art keywords
purified
tnfr
purifying
protein
proteins containing
Prior art date
Application number
BR112014000352A
Other languages
English (en)
Inventor
Lee Christine
Cynthia Ikechukwu Ijeoma
Nti-Gyabaah Joseph
Petroff Matthew
Original Assignee
Merck Sharp & Dohe Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp & Dohe Corp filed Critical Merck Sharp & Dohe Corp
Publication of BR112014000352A2 publication Critical patent/BR112014000352A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
BR112014000352A 2011-07-08 2012-07-03 método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada BR112014000352A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161505710P 2011-07-08 2011-07-08
PCT/US2012/045339 WO2013009526A1 (en) 2011-07-08 2012-07-03 Method for purifying fc-fusion protein

Publications (1)

Publication Number Publication Date
BR112014000352A2 true BR112014000352A2 (pt) 2017-02-14

Family

ID=47506409

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014000352A BR112014000352A2 (pt) 2011-07-08 2012-07-03 método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada

Country Status (10)

Country Link
US (1) US9556258B2 (pt)
EP (1) EP2729482B1 (pt)
JP (1) JP6280499B2 (pt)
KR (1) KR20140043934A (pt)
CN (1) CN103814044A (pt)
AU (2) AU2012282960A1 (pt)
BR (1) BR112014000352A2 (pt)
CA (1) CA2840951A1 (pt)
DK (1) DK2729482T3 (pt)
WO (1) WO2013009526A1 (pt)

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WO2013059412A1 (en) 2011-10-18 2013-04-25 Coherus Biosciences, Inc. Etanercept formulations stabilized with combinations of sugars and polyols
CN104902914B (zh) 2012-09-11 2019-01-01 科荣生生物科学公司 高纯度和优异产量的正确折叠的依那西普
DK3395423T3 (da) * 2013-03-14 2023-11-20 Amgen Inc Fjernelse af lækket affinitetsoprensningsligand
EP3391942A1 (en) * 2013-03-26 2018-10-24 Coherus Biosciences, Inc. Protein production method
AU2015214245B2 (en) 2014-02-04 2020-09-10 Biogen Ma Inc. Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 官能化担体材料並びに官能化担体材料を作製及び使用する方法
EA202190762A1 (ru) 2014-05-13 2021-10-29 Эмджен Инк. Системы и способы управления технологическим процессом для использования с фильтрами и в процессах фильтрации
BR112016029157A8 (pt) 2014-06-13 2021-07-06 Lupin Ltd processo para purificar a proteína de fusão tnfr:fc
FR3025515B1 (fr) 2014-09-05 2016-09-09 Lab Francais Du Fractionnement Procede de purification d'un anticorps monoclonal
US11053504B2 (en) * 2014-10-24 2021-07-06 The University Of Chicago Heat-inducible self-assembling protein domains
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
WO2016108569A1 (ko) * 2014-12-31 2016-07-07 주식회사 엘지생명과학 목적하는 함량으로 불순물을 포함하는 tnfr-fc 융합 단백질의 제조방법
KR102566292B1 (ko) * 2015-06-05 2023-08-10 더블유.알. 그레이스 앤드 캄파니-콘. 흡착성 바이오프로세싱 정화제와 이의 제조 및 사용 방법
US20170101435A1 (en) * 2015-10-13 2017-04-13 Therapeutic Proteins International, LLC Harvesting and perfusion apparatus
ES2927271T3 (es) 2015-11-19 2022-11-03 Takeda Pharmaceuticals Co Inhibidor de esterasa C1 humana recombinante y usos del mismo
CN109311948B (zh) 2016-05-11 2022-09-16 思拓凡生物工艺研发有限公司 清洁和/或消毒分离基质的方法
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US11708390B2 (en) 2016-05-11 2023-07-25 Cytiva Bioprocess R&D Ab Method of storing a separation matrix
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
ES2874974T3 (es) 2016-05-11 2021-11-05 Cytiva Bioprocess R & D Ab Matriz de separación
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
JP7092744B2 (ja) 2016-07-22 2022-06-28 アムジエン・インコーポレーテツド Ｆｃ含有タンパク質を精製する方法
CN109929027B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 采用线性洗脱步骤的重组融合蛋白纯化方法
CN109929038B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
US11416468B2 (en) * 2020-07-21 2022-08-16 International Business Machines Corporation Active-active system index management
CN112574321B (zh) * 2020-12-30 2023-10-20 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法
CA3233422A1 (en) * 2021-09-28 2023-04-06 Kashiv Biosciences, Llc An improved process for purification of fusion protein

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EP0972023A2 (en) 1997-01-21 2000-01-19 Human Genome Sciences Fc RECEPTORS AND POLYPEPTIDES
EP1006183A1 (en) 1998-12-03 2000-06-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Recombinant soluble Fc receptors
AU2002359816B2 (en) * 2001-12-21 2006-07-13 Immunex Corporation Methods for purifying protein
EP1543106B1 (en) * 2002-07-15 2007-04-11 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
CA2542951C (en) * 2003-10-24 2016-08-30 Abhinav A. Shukla Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
MXPA06009496A (es) * 2004-04-14 2007-04-09 Hoffmann La Roche Proteina de fusion interleukina-15/fc purificada y preparacion de la misma.
JP2010501622A (ja) * 2006-08-28 2010-01-21 アレス トレーディング ソシエテ アノニム Ｆｃ−融合タンパク質の精製法
EP2061802A1 (en) * 2006-08-28 2009-05-27 Ares Trading S.A. Process for the purification of fc-fusion proteins
WO2008085988A1 (en) * 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
ES2391931T3 (es) 2008-09-15 2012-12-03 Emd Millipore Corporation Procedimientos para cuantificar la filtración de proteína desde resinas de cromatografía de afinidad basada en proteína
US20120282654A1 (en) 2009-04-29 2012-11-08 Schering Corporation Antibody purification
ES2817802T3 (es) 2009-08-07 2021-04-08 Emd Millipore Corp Métodos para purificar una proteína objetivo de una o más impurezas en una muestra
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production

Also Published As

Publication number Publication date
WO2013009526A1 (en) 2013-01-17
EP2729482B1 (en) 2018-03-07
CA2840951A1 (en) 2013-01-17
US9556258B2 (en) 2017-01-31
KR20140043934A (ko) 2014-04-11
AU2012282960A1 (en) 2014-01-16
US20140187751A1 (en) 2014-07-03
EP2729482A4 (en) 2015-02-25
AU2017204893A1 (en) 2017-08-03
JP6280499B2 (ja) 2018-02-14
EP2729482A1 (en) 2014-05-14
DK2729482T3 (en) 2018-05-07
JP2014520814A (ja) 2014-08-25
CN103814044A (zh) 2014-05-21

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NAO 10196/2001, QUE MODIFICOU A LEI NAO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUAANCIA PRA VIA DA ANVISA. CONSIDERANDO A APROVAA AO DOS TERMOS DO PARECER NAO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NAO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDAANCIAS CABA-VEIS.

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 8A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2594 DE 24-09-2020 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.