ATE359358T1 - Verfahren und medien zur kontrolle der sialysierung von proteinen, die von säugetierzellen produziert werden - Google Patents
Verfahren und medien zur kontrolle der sialysierung von proteinen, die von säugetierzellen produziert werdenInfo
- Publication number
- ATE359358T1 ATE359358T1 AT03764511T AT03764511T ATE359358T1 AT E359358 T1 ATE359358 T1 AT E359358T1 AT 03764511 T AT03764511 T AT 03764511T AT 03764511 T AT03764511 T AT 03764511T AT E359358 T1 ATE359358 T1 AT E359358T1
- Authority
- AT
- Austria
- Prior art keywords
- cells
- media
- sialylysis
- controlling
- proteins produced
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 241000124008 Mammalia Species 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 4
- 238000012258 culturing Methods 0.000 abstract 3
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 abstract 2
- 229930091371 Fructose Natural products 0.000 abstract 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 abstract 2
- 239000005715 Fructose Substances 0.000 abstract 2
- OVRNDRQMDRJTHS-OZRXBMAMSA-N N-acetyl-beta-D-mannosamine Chemical compound CC(=O)N[C@@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-OZRXBMAMSA-N 0.000 abstract 2
- 229930182830 galactose Natural products 0.000 abstract 2
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 230000009450 sialylation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
Landscapes
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39622102P | 2002-07-15 | 2002-07-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE359358T1 true ATE359358T1 (de) | 2007-05-15 |
Family
ID=30115987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03764511T ATE359358T1 (de) | 2002-07-15 | 2003-07-14 | Verfahren und medien zur kontrolle der sialysierung von proteinen, die von säugetierzellen produziert werden |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US7645609B2 (de) |
| EP (1) | EP1543106B1 (de) |
| JP (1) | JP4510938B2 (de) |
| AT (1) | ATE359358T1 (de) |
| AU (1) | AU2003249055B2 (de) |
| CA (1) | CA2492267C (de) |
| CY (1) | CY1107689T1 (de) |
| DE (1) | DE60313188T2 (de) |
| DK (1) | DK1543106T3 (de) |
| ES (1) | ES2285188T3 (de) |
| MX (1) | MXPA05000552A (de) |
| PL (1) | PL376381A1 (de) |
| PT (1) | PT1543106E (de) |
| SI (1) | SI1543106T1 (de) |
| WO (1) | WO2004008100A2 (de) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MXPA05006522A (es) † | 2002-12-23 | 2006-02-17 | Bristol Myers Squibb Co | Mejora en la calidad de producto en procesos de cultivo en celulas de mamiferos para la produccion de proteina. |
| CA2621250A1 (en) | 2004-09-07 | 2006-03-16 | Zymequest, Inc. | Apparatus for prolonging survival of platelets |
| CA2626363A1 (en) * | 2004-10-15 | 2006-04-27 | Zymequest, Inc. | Compositions and methods for prolonging survival of platelets |
| ATE450615T1 (de) | 2005-07-22 | 2009-12-15 | Five Prime Therapeutics Inc | Zusammensetzungen und verfahren zur behandlung von krankheiten mit fgfr-fusionsproteinen |
| CA2619825A1 (en) * | 2005-08-19 | 2007-03-01 | Centocor, Inc. | Proteolysis resistant antibody preparations |
| EP1954304A4 (de) * | 2005-10-14 | 2010-03-10 | Zymequest Inc | Zusammensetzungen und verfahren zur verlängerung der lebensdauer von plättchen |
| EP1969007B1 (de) | 2005-12-20 | 2013-08-28 | Bristol-Myers Squibb Company | Zusammensetzungen und verfahren zur herstellung einer zusammensetzung |
| AR058568A1 (es) | 2005-12-20 | 2008-02-13 | Bristol Myers Squibb Co | Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo |
| DK2041270T3 (da) | 2006-07-13 | 2014-01-27 | Wyeth Llc | Fremstilling af glycoproteiner |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| NZ597334A (en) | 2006-09-13 | 2014-02-28 | Abbott Lab | Cell culture improvements |
| KR101248252B1 (ko) | 2006-11-28 | 2013-03-27 | 한올바이오파마주식회사 | 변형된 에리스로포이에틴 폴리펩티드와 이의 치료용 용도 |
| WO2008120570A1 (ja) * | 2007-03-19 | 2008-10-09 | National University Corporation Okayama University | タンパク質産生用およびウイルス増殖用の培地 |
| TW200902708A (en) | 2007-04-23 | 2009-01-16 | Wyeth Corp | Methods of protein production using anti-senescence compounds |
| CN102171343B (zh) | 2008-08-04 | 2017-07-14 | 戊瑞治疗有限公司 | Fgfr细胞外结构域酸性区突变蛋白 |
| MX2011004201A (es) | 2008-10-20 | 2011-05-24 | Abbott Lab | Desactivacion viral durante purificacion de anticuerpos. |
| AU2009347206C1 (en) | 2008-10-20 | 2016-12-08 | Abbvie Inc. | Isolation and purification of antibodies using Protein A affinity chromatography |
| EP2233502A1 (de) * | 2009-03-27 | 2010-09-29 | Deutsches Rheuma-Forschungszentrum Berlin | Sialylierte antigenspezifische Antikörper zur Behandlung oder Vorbeugung von ungewollten entzündlichen Immunreaktionen und Verfahren zu deren Herstellung |
| WO2011034940A1 (en) | 2009-09-15 | 2011-03-24 | Five Prime Therapeutics, Inc. | Hair growth methods using fgfr4 extracellular domains |
| KR101808751B1 (ko) | 2009-11-13 | 2017-12-13 | 그리폴스 테라퓨틱스 인코포레이티드 | 폰 빌레브란트 인자(ｖＷＦ)-함유 제제, 및 그와 관련된 방법, 키트 및 용도 |
| PT2498799T (pt) | 2009-11-13 | 2016-11-04 | Five Prime Therapeutics Inc | Utilização de proteínas do domínio extracelular rfcf1 para tratar cancros caracterizados por mutações ativadoras dependentes de ligando em rfcf2 |
| EP2325296A1 (de) | 2009-11-20 | 2011-05-25 | LEK Pharmaceuticals d.d. | Herstellung von Glykoproteinen mit niedrigem N-Glykolylneuraminsäure-Gehalt (Neu5Gc-Gehalt) |
| US8685931B2 (en) | 2009-12-17 | 2014-04-01 | Five Prime Therapeutics, Inc. | Hair growth methods using FGFR3 extracellular domains |
| EP2563904B1 (de) | 2010-04-26 | 2015-01-21 | Novartis AG | Verbessertes zellkulturmedium |
| US8481038B2 (en) | 2010-11-15 | 2013-07-09 | Five Prime Therapeutics, Inc. | Treatment of cancer with elevated dosages of soluble FGFR1 fusion proteins |
| US8951972B2 (en) | 2010-12-09 | 2015-02-10 | Five Prime Therapeutics, Inc. | FGFR1 extracellular domain combination therapies for lung cancer |
| EP2702077A2 (de) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Verfahren zur steuerung des galactosylierungsprofil von rekombinant exprimierten proteinen |
| WO2013009526A1 (en) * | 2011-07-08 | 2013-01-17 | Merck Sharp & Dohme Corp. | Method for purifying fc-fusion protein |
| EP2780033B1 (de) | 2011-11-14 | 2019-05-08 | Five Prime Therapeutics, Inc. | Eine fgfr1 extrazelluläre domäne zur behandlung von krebs mit einer fgfr1 gen vervielfältigung |
| US9334319B2 (en) | 2012-04-20 | 2016-05-10 | Abbvie Inc. | Low acidic species compositions |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| US9181572B2 (en) | 2012-04-20 | 2015-11-10 | Abbvie, Inc. | Methods to modulate lysine variant distribution |
| US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| KR20150043523A (ko) | 2012-09-02 | 2015-04-22 | 애브비 인코포레이티드 | 단백질 불균일성의 제어 방법 |
| US20140106405A1 (en) * | 2012-10-15 | 2014-04-17 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
| WO2014143205A1 (en) | 2013-03-12 | 2014-09-18 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| TWI771890B (zh) * | 2013-03-14 | 2022-07-21 | 美商安美基公司 | 用於增加重組蛋白質之甘露糖含量之方法 |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| US9499614B2 (en) * | 2013-03-14 | 2016-11-22 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides |
| US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
| US8956830B2 (en) | 2013-03-14 | 2015-02-17 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
| JP6689189B2 (ja) | 2013-03-26 | 2020-04-28 | コヒラス・バイオサイエンシズ・インコーポレイテッド | タンパク質産生方法 |
| US9481901B2 (en) | 2013-05-30 | 2016-11-01 | Amgen Inc. | Methods for increasing mannose content of recombinant proteins |
| WO2015051293A2 (en) | 2013-10-04 | 2015-04-09 | Abbvie, Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| EP3113781B1 (de) * | 2014-03-05 | 2018-11-21 | Ultragenyx Pharmaceutical Inc. | Sialylierte glycoproteinzusammensetzungen und verwendungen davon |
| JP6652334B2 (ja) * | 2014-05-31 | 2020-02-19 | Ｊｃｒファーマ株式会社 | ウリジンとｎ−アセチル−ｄ−マンノサミンとを含有する培地 |
| US20160130324A1 (en) | 2014-10-31 | 2016-05-12 | Shire Human Genetic Therapies, Inc. | C1 Inhibitor Fusion Proteins and Uses Thereof |
| MA43270A (fr) | 2015-11-19 | 2021-06-02 | Takeda Pharmaceuticals Co | Inhibiteur de la c1 estérase humaine recombinante et ses utilisations |
| IL307155B2 (en) | 2018-05-01 | 2025-11-01 | Amgen Inc | Antibodies with modulated glycan profiles |
| KR102579246B1 (ko) | 2018-07-03 | 2023-09-19 | 길리애드 사이언시즈, 인코포레이티드 | HIV gp120을 표적화하는 항체 및 사용 방법 |
| CN113748129A (zh) * | 2018-12-21 | 2021-12-03 | 杭州景杰生物科技股份有限公司 | 蛋白质赖氨酸乳酸酰化检测试剂和方法 |
| EP4267717A1 (de) | 2020-12-22 | 2023-11-01 | Amgen Inc. | Zellkulturverfahren |
| CA3205815A1 (en) | 2021-03-03 | 2022-09-09 | Alwin REITER | Formulations of ace2 fc fusion proteins |
| US20250027067A1 (en) | 2021-11-24 | 2025-01-23 | Formycon Ag | Improved ace2 fusion proteins |
| CN114317446B (zh) * | 2021-12-30 | 2023-12-05 | 广州康盛生物科技股份有限公司 | 一种无血清培养基用添加剂及其应用 |
| EP4331571A1 (de) | 2022-09-02 | 2024-03-06 | Formycon AG | Formulierungen von ace2-igm-fusionsproteinen |
| EP4386084A1 (de) | 2022-12-14 | 2024-06-19 | Formycon AG | Verbesserte ace2-fusionsproteine |
| WO2025038362A1 (en) | 2023-08-11 | 2025-02-20 | Merck Sharp & Dohme Llc | Monovalent interleukin 12 (il-12) heterodimeric fc proteins |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5672502A (en) * | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
| US5047335A (en) * | 1988-12-21 | 1991-09-10 | The Regents Of The University Of Calif. | Process for controlling intracellular glycosylation of proteins |
| JP3375140B2 (ja) | 1991-03-08 | 2003-02-10 | 森永製菓株式会社 | モノクローナル抗体生産用培地の作製法及びそのキット |
| DE69311873T2 (de) * | 1992-05-01 | 1998-02-12 | Teijin Ltd | Fed-batch-Verfahren für Proteine sekretierende Zellen |
| JPH06292592A (ja) * | 1993-02-09 | 1994-10-21 | Snow Brand Milk Prod Co Ltd | 糖蛋白質の生産方法 |
| US5858783A (en) * | 1993-05-25 | 1999-01-12 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Production of normal mammalian organ culture using a medium containing mem-alpha, leibovitz L-15, glucose galactose fructose |
| EP0911416B1 (de) * | 1997-10-01 | 2006-05-17 | DSM IP Assets B.V. | Verfahren zur Protein-Herstellung |
| DE59813187D1 (de) * | 1997-12-03 | 2005-12-15 | Roche Diagnostics Gmbh | Verfahren zur herstellung von polypeptiden mit geeigneter glykosilierung |
| ES2523599T3 (es) | 1997-12-03 | 2014-11-27 | Roche Diagnostics Gmbh | Eritropoyetina de gran actividad específica |
| WO2000007602A1 (en) * | 1998-08-06 | 2000-02-17 | The Johns Hopkins University School Of Medicine | Compounds for altering cell surface sialic acids and methods of use therefor |
| DE19852729A1 (de) | 1998-11-16 | 2000-05-18 | Werner Reutter | Rekombinante Glycoproteine, Verfahren zu ihrer Herstellung, sie enthaltende Arzneimittel und ihre Verwendung |
| US6949372B2 (en) * | 1999-03-02 | 2005-09-27 | The Johns Hopkins University | Engineering intracellular sialylation pathways |
| US6261805B1 (en) * | 1999-07-15 | 2001-07-17 | Boyce Thompson Institute For Plant Research, Inc. | Sialyiation of N-linked glycoproteins in the baculovirus expression vector system |
| WO2002002793A1 (fr) * | 2000-07-05 | 2002-01-10 | Japan As Represented By Secretary Of Osaka University | Processus de production de glycoproteine |
| US20040214228A9 (en) * | 2001-09-14 | 2004-10-28 | Ganesh Venkataraman | Methods of evaluating glycomolecules for enhanced activities |
| JP4281797B2 (ja) | 2006-12-21 | 2009-06-17 | 三菱自動車工業株式会社 | 車両の車体構造 |
-
2003
- 2003-07-14 MX MXPA05000552A patent/MXPA05000552A/es active IP Right Grant
- 2003-07-14 JP JP2004521682A patent/JP4510938B2/ja not_active Expired - Lifetime
- 2003-07-14 SI SI200330783T patent/SI1543106T1/sl unknown
- 2003-07-14 DE DE60313188T patent/DE60313188T2/de not_active Expired - Lifetime
- 2003-07-14 ES ES03764511T patent/ES2285188T3/es not_active Expired - Lifetime
- 2003-07-14 PT PT03764511T patent/PT1543106E/pt unknown
- 2003-07-14 EP EP03764511A patent/EP1543106B1/de not_active Expired - Lifetime
- 2003-07-14 AU AU2003249055A patent/AU2003249055B2/en not_active Expired
- 2003-07-14 DK DK03764511T patent/DK1543106T3/da active
- 2003-07-14 CA CA2492267A patent/CA2492267C/en not_active Expired - Lifetime
- 2003-07-14 PL PL03376381A patent/PL376381A1/xx not_active Application Discontinuation
- 2003-07-14 WO PCT/US2003/021733 patent/WO2004008100A2/en not_active Ceased
- 2003-07-14 AT AT03764511T patent/ATE359358T1/de not_active IP Right Cessation
- 2003-07-15 US US10/620,064 patent/US7645609B2/en not_active Expired - Lifetime
-
2007
- 2007-07-02 CY CY20071100866T patent/CY1107689T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US7645609B2 (en) | 2010-01-12 |
| MXPA05000552A (es) | 2005-04-28 |
| AU2003249055A1 (en) | 2004-02-02 |
| EP1543106B1 (de) | 2007-04-11 |
| EP1543106A4 (de) | 2005-08-10 |
| AU2003249055B2 (en) | 2008-06-26 |
| ES2285188T3 (es) | 2007-11-16 |
| WO2004008100A2 (en) | 2004-01-22 |
| PL376381A1 (en) | 2005-12-27 |
| DK1543106T3 (da) | 2007-08-13 |
| US20040115768A1 (en) | 2004-06-17 |
| CA2492267A1 (en) | 2004-01-22 |
| PT1543106E (pt) | 2007-05-31 |
| JP4510938B2 (ja) | 2010-07-28 |
| JP2005532813A (ja) | 2005-11-04 |
| CY1107689T1 (el) | 2013-04-18 |
| SI1543106T1 (sl) | 2007-08-31 |
| WO2004008100A3 (en) | 2004-03-25 |
| EP1543106A2 (de) | 2005-06-22 |
| DE60313188D1 (de) | 2007-05-24 |
| CA2492267C (en) | 2013-09-10 |
| DE60313188T2 (de) | 2007-12-20 |
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| Date | Code | Title | Description |
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| UEP | Publication of translation of european patent specification |
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| REN | Ceased due to non-payment of the annual fee |