ATE553187T1 - Konditioniertes zellkulturmedium, verfahren zur herstellung und dessen verwendung zur haltung, proliferation und differenzierung von säugetierzellen - Google Patents

Konditioniertes zellkulturmedium, verfahren zur herstellung und dessen verwendung zur haltung, proliferation und differenzierung von säugetierzellen

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Publication number
ATE553187T1
ATE553187T1 AT04766461T AT04766461T ATE553187T1 AT E553187 T1 ATE553187 T1 AT E553187T1 AT 04766461 T AT04766461 T AT 04766461T AT 04766461 T AT04766461 T AT 04766461T AT E553187 T1 ATE553187 T1 AT E553187T1
Authority
AT
Austria
Prior art keywords
proliferation
differentiation
cell culture
culture medium
cells
Prior art date
Application number
AT04766461T
Other languages
English (en)
Inventor
Veronica Bordoni
Tonino Alonzi
Marco Tripodi
Original Assignee
Istituto Naz Per Le Malattie Infettive Lazzaro Spallanzani Istituto Di Ricovero E Cura A Carattere S
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Application filed by Istituto Naz Per Le Malattie Infettive Lazzaro Spallanzani Istituto Di Ricovero E Cura A Carattere S filed Critical Istituto Naz Per Le Malattie Infettive Lazzaro Spallanzani Istituto Di Ricovero E Cura A Carattere S
Application granted granted Critical
Publication of ATE553187T1 publication Critical patent/ATE553187T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • C12N5/0647Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/14Coculture with; Conditioned medium produced by hepatocytes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Veterinary Medicine (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT04766461T 2003-08-12 2004-08-10 Konditioniertes zellkulturmedium, verfahren zur herstellung und dessen verwendung zur haltung, proliferation und differenzierung von säugetierzellen ATE553187T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT000395A ITRM20030395A1 (it) 2003-08-12 2003-08-12 Terreno di coltura per il mantenimento, la proliferazione e il differenziamento di cellule di mammifero.
PCT/EP2004/051758 WO2005014799A1 (en) 2003-08-12 2004-08-10 Conditioned cell culture medium, method to obtain the same and use of it for maintenance, proliferation and differentiation of mammalian cells

Publications (1)

Publication Number Publication Date
ATE553187T1 true ATE553187T1 (de) 2012-04-15

Family

ID=30131547

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04766461T ATE553187T1 (de) 2003-08-12 2004-08-10 Konditioniertes zellkulturmedium, verfahren zur herstellung und dessen verwendung zur haltung, proliferation und differenzierung von säugetierzellen

Country Status (5)

Country Link
US (1) US7723105B2 (de)
EP (1) EP1656446B1 (de)
AT (1) ATE553187T1 (de)
IT (1) ITRM20030395A1 (de)
WO (1) WO2005014799A1 (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
PL1888123T3 (pl) 2005-06-08 2013-06-28 Janssen Biotech Inc Terapia komórkowa chorób degeneracyjnych oka
EP2021462B1 (de) 2006-04-28 2019-01-09 Lifescan, Inc. Differenzierung menschlicher embryonischer stammzellen
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
CN101641436A (zh) 2007-01-30 2010-02-03 佐治亚大学研究基金会 用于产生内胚层和中胚层细胞系及多能游走细胞(mmc)的早期中胚层细胞即稳定的中内胚层细胞群
WO2008137115A1 (en) 2007-05-03 2008-11-13 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
US8574567B2 (en) 2007-05-03 2013-11-05 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
DK3192865T3 (da) 2007-07-01 2021-06-28 Janssen Biotech Inc Enkelt pluripotent stamcelle-kultur
US8855567B2 (en) * 2007-07-10 2014-10-07 Qualcomm Incorporated Methods and apparatus for successive interference cancellation based on two rate feedback in peer-to-peer networks
US8433349B2 (en) * 2007-07-10 2013-04-30 Qualcomm Incorporated Methods and apparatus for successive interference cancellation based on transmit power control by interfering device with success probability adaptation in peer-to-peer wireless networks
US8874040B2 (en) * 2007-07-10 2014-10-28 Qualcomm Incorporated Methods and apparatus for successive interference cancellation based on rate capping in peer-to-peer networks
US8849197B2 (en) * 2007-07-10 2014-09-30 Qualcomm Incorporated Methods and apparatus for active successive interference cancellation in peer-to-peer networks
US9668225B2 (en) * 2007-07-10 2017-05-30 Qualcomm Incorporated Methods and apparatus for active successive interference cancellation based on one rate feedback and probability adaptation in peer-to-peer networks
CN101861386A (zh) 2007-07-18 2010-10-13 生命扫描有限公司 人胚胎干细胞的分化
EP2584034B8 (de) 2007-07-31 2017-12-06 Lifescan, Inc. Differenzierung pluripotenter stammzellen durch verwendung menschlicher feederzellen
KR101617243B1 (ko) 2007-07-31 2016-05-02 라이프스캔, 인코포레이티드 인간 배아 줄기 세포의 분화
MX2010005805A (es) 2007-11-27 2010-06-09 Lifescan Inc Diferenciacion de celulas madre embrionarias humanas.
JP5733986B2 (ja) 2008-02-21 2015-06-10 ヤンセン バイオテツク,インコーポレーテツド 細胞の付着、培養、及び剥離のための方法、表面改質されたプレート、並びに組成物
US7939322B2 (en) 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
KR101651661B1 (ko) 2008-06-30 2016-08-26 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
US20110305672A1 (en) 2008-07-25 2011-12-15 University Of Georgia Research Foundation, Inc. COMPOSITIONS FOR MESODERM DERIVED ISL1+ MULTIPOTENT CELLS (IMPs), EPICARDIAL PROGENITOR CELLS (EPCs) AND MULTIPOTENT CD56C CELLS (C56Cs) AND METHODS OF PRODUCING AND USING SAME
CA2731821A1 (en) 2008-07-25 2010-01-28 Stephen Dalton Compositions for mesoderm derived isl1+ multipotent cells (imps), epicardial progenitor cells (epcs) and multipotent cxcr4+cd56+ cells (c56cs) and methods of use
US9012218B2 (en) 2008-10-31 2015-04-21 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
PL2346988T3 (pl) 2008-10-31 2017-10-31 Janssen Biotech Inc Różnicowanie ludzkich zarodkowych komórek macierzystych do linii endokrynnej trzustki
KR101774546B1 (ko) 2008-11-20 2017-09-04 얀센 바이오테크 인코포레이티드 마이크로-캐리어 상의 만능 줄기 세포 배양
JP5719305B2 (ja) 2008-11-20 2015-05-13 ヤンセン バイオテツク,インコーポレーテツド 平面支持体上での細胞付着及び培養のための方法及び組成物
WO2010115634A1 (en) 2009-04-09 2010-10-14 Cellca Gmbh Method for improved single cell cloning
CN102482643B (zh) 2009-07-20 2016-06-29 詹森生物科技公司 人胚胎干细胞的分化
EP2456859A4 (de) 2009-07-20 2015-03-18 Janssen Biotech Inc Differenzierung menschlicher embryonischer stammzellen
EP2456858B1 (de) 2009-07-20 2018-08-29 Janssen Biotech, Inc. Differenzierung menschlicher embryonischer stammzellen
EP2516626B1 (de) 2009-12-23 2017-05-10 Janssen Biotech, Inc. Differenzierung menschlicher embryonischer stammzellen
CN102741395B (zh) 2009-12-23 2016-03-16 詹森生物科技公司 人胚胎干细胞的分化
JP6013196B2 (ja) 2010-03-01 2016-10-25 ヤンセン バイオテツク,インコーポレーテツド 多能性幹細胞から誘導した細胞を精製するための方法
AU2011250912A1 (en) 2010-05-12 2012-11-22 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
DK2603226T3 (da) 2010-08-12 2021-06-07 Janssen Biotech Inc Behandling af diabetes med endokrine pankreas-prækursorceller
CA2809300A1 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
EP2611907B1 (de) 2010-08-31 2016-05-04 Janssen Biotech, Inc. Differenzierung pluripotenter stammzellen
AU2011296382B2 (en) 2010-08-31 2016-04-14 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
SG10201608914WA (en) 2011-12-22 2016-12-29 Janssen Biotech Inc Differentiation of human embryonic stem cells into single hormonal insulin positive cells
KR20140131999A (ko) 2012-03-07 2014-11-14 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 증폭 및 유지를 위한 한정 배지
US10066210B2 (en) 2012-06-08 2018-09-04 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
KR101942769B1 (ko) 2012-12-31 2019-01-28 얀센 바이오테크 인코포레이티드 Hb9 조절제를 사용하는 인간 배아 줄기세포의 췌장 내분비 세포로의 분화
BR112015015714A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc suspensão e aglomeração de células pluripotentes humanas para diferenciação em célu-las endócrinas pancreáticas
SG10201709338RA (en) 2012-12-31 2017-12-28 Janssen Biotech Inc Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
CN117821369A (zh) 2014-05-16 2024-04-05 詹森生物科技公司 小分子增强胰腺内分泌细胞中的mafa表达的用途
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
CN107841481A (zh) * 2016-09-19 2018-03-27 深圳华大方舟生物技术有限公司 一种用于单细胞培养的培养基及其制备方法和应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2917998B2 (ja) * 1988-02-05 1999-07-12 ホワイトヘッド・インスティチュート・フォー・バイオメディカル・リサーチ 修飾された肝細胞およびその用途
US6284236B1 (en) * 1995-06-29 2001-09-04 Immunex Corporation Cytokine that induces apoptosis
US6372494B1 (en) * 1999-05-14 2002-04-16 Advanced Tissue Sciences, Inc. Methods of making conditioned cell culture medium compositions
ITTO20020729A1 (it) 2002-08-14 2004-02-15 Consorzio Interuniversitario Per Le Biotecnologie Cellule di mammifero non umano geneticamente modificate, procedimento per la loro produzione e utilizzo in test di tossicita'.

Also Published As

Publication number Publication date
ITRM20030395A1 (it) 2005-02-13
WO2005014799A1 (en) 2005-02-17
EP1656446B1 (de) 2012-04-11
ITRM20030395A0 (it) 2003-08-12
US7723105B2 (en) 2010-05-25
US20060286081A1 (en) 2006-12-21
EP1656446A1 (de) 2006-05-17

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