BR0014519A - Sequências de sinais para a preparação de leu-herudin através da secreção por e. coli no meio de cultura - Google Patents

Sequências de sinais para a preparação de leu-herudin através da secreção por e. coli no meio de cultura

Info

Publication number
BR0014519A
BR0014519A BR0014519-0A BR0014519A BR0014519A BR 0014519 A BR0014519 A BR 0014519A BR 0014519 A BR0014519 A BR 0014519A BR 0014519 A BR0014519 A BR 0014519A
Authority
BR
Brazil
Prior art keywords
coli
leu
preparation
herudin
signal sequences
Prior art date
Application number
BR0014519-0A
Other languages
English (en)
Inventor
Paul Habermann
Rudolf Bender
Original Assignee
Aventis Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Gmbh filed Critical Aventis Pharma Gmbh
Publication of BR0014519A publication Critical patent/BR0014519A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Patente de Invenção: "SEQuêNCIAS DE SINAIS PARA A PREPARAçãO DE LEU-HERUDIN ATRAVéS DA SECREçãO POR E. COLI NO MEIO DE CULTURA". A presente invenção refere-se a um precursor de hirundina contendo uma seq³ência de sinal e a seq³ência de Leu-Hirundina, sua preparação e emprego, bem como um processo para a determinação de seq³ências de sinais para a expressão secretora de proteínas desejadas em E. coli e processos para a expressão secretora de proteínas desejadas em E. coli.
BR0014519-0A 1999-09-18 2000-09-01 Sequências de sinais para a preparação de leu-herudin através da secreção por e. coli no meio de cultura BR0014519A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19944870A DE19944870A1 (de) 1999-09-18 1999-09-18 Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
PCT/EP2000/008537 WO2001021662A1 (de) 1999-09-18 2000-09-01 Signalsequenzen zur herstellung von leu-hirudin über sekretion durch e. coli in das kulturmedium

Publications (1)

Publication Number Publication Date
BR0014519A true BR0014519A (pt) 2002-06-11

Family

ID=7922547

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0014519-0A BR0014519A (pt) 1999-09-18 2000-09-01 Sequências de sinais para a preparação de leu-herudin através da secreção por e. coli no meio de cultura

Country Status (29)

Country Link
US (1) US7455987B1 (pt)
EP (1) EP1216259B1 (pt)
JP (1) JP4669181B2 (pt)
KR (1) KR100737189B1 (pt)
CN (2) CN1192037C (pt)
AR (1) AR025660A1 (pt)
AT (1) ATE357458T1 (pt)
AU (1) AU775923B2 (pt)
BR (1) BR0014519A (pt)
CA (1) CA2385287C (pt)
CZ (1) CZ2002899A3 (pt)
DE (2) DE19944870A1 (pt)
DK (1) DK1216259T3 (pt)
EE (1) EE05187B1 (pt)
HK (2) HK1049172A1 (pt)
HR (1) HRP20020226B1 (pt)
HU (1) HUP0202596A3 (pt)
IL (2) IL148624A0 (pt)
ME (1) MEP27908A (pt)
MX (1) MXPA02002469A (pt)
NO (1) NO329723B1 (pt)
NZ (1) NZ517825A (pt)
PL (1) PL205117B1 (pt)
RS (1) RS50435B (pt)
RU (1) RU2261867C2 (pt)
SK (1) SK287865B6 (pt)
TR (2) TR200200667T2 (pt)
WO (1) WO2001021662A1 (pt)
ZA (1) ZA200202106B (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
MXPA06005705A (es) 2003-11-21 2006-08-23 Dow Global Technologies Inc Sistemas de expresion mejorada con secrecion de sistema de sec.
AU2005269527B2 (en) 2004-07-26 2011-12-01 Pfenex Inc. Process for improved protein expression by strain engineering
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
DE102006044841A1 (de) * 2006-09-22 2008-04-03 Wacker Chemie Ag Signalpeptid zur Produktion von rekombinanten Proteinen
CN102517280A (zh) 2007-01-31 2012-06-27 菲尼克斯股份有限公司 用于提高表达的细菌前导序列
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
CA2685326A1 (en) 2007-04-27 2008-11-06 Dow Global Technologies Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2968470B1 (en) 2013-03-12 2020-10-28 The General Hospital Corporation Modified mullerian inhibiting substance (mis) proteins and uses thereof for the treatment of diseases
EP3079712B1 (en) 2013-12-11 2022-02-02 The General Hospital Corporation Use of mullerian inhibiting substance (mis) proteins for contraception and ovarian reserve preservation
CN108220369B (zh) * 2017-12-04 2021-03-12 广东双骏生物科技有限公司 一种生产重组水蛭素的方法
CN108359689B (zh) * 2018-01-19 2021-03-23 华中农业大学 利用抗病基因识别无毒效应蛋白以鉴定具有分泌功能信号肽的方法
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
EP0324712B1 (de) * 1985-04-11 1993-04-07 Hoechst Aktiengesellschaft Hirudin-Derivat
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
AU604925B2 (en) * 1988-02-23 1991-01-03 Schering Aktiengesellschaft A hirudin derivative
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
JPH07119237B2 (ja) * 1990-11-08 1995-12-20 株式会社ジャパンエナジー ヒルジン変異体，その製造法及び抗凝血剤
WO1992008736A1 (fr) * 1990-11-08 1992-05-29 Nippon Mining Company Limited Mutant d'hirudine, sa production, anticoagulant, vecteur secretoire, microorganisme transforme par ledit vecteur et production d'un produit a partir dudit microorganisme
US5389529A (en) * 1991-06-12 1995-02-14 Regeneron Pharmaceuticals, Inc. Modified lamβ signal sequence and processes for producing recombinant neurotrophins
NZ243084A (en) * 1991-06-12 1995-08-28 Regeneron Pharma Production and recovery of recombinant neurotrophins, genes for a modified lamb signal sequence, fusion genes and plasmids
DE4140381A1 (de) 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet

Also Published As

Publication number Publication date
AU6842100A (en) 2001-04-24
EE200200139A (et) 2003-06-16
US7455987B1 (en) 2008-11-25
HRP20020226A2 (en) 2004-04-30
TR200501541T2 (tr) 2005-06-21
NO20021254D0 (no) 2002-03-13
EP1216259B1 (de) 2007-03-21
MXPA02002469A (es) 2002-08-28
JP4669181B2 (ja) 2011-04-13
DE19944870A1 (de) 2001-03-29
HK1049172A1 (en) 2003-05-02
EP1216259A1 (de) 2002-06-26
ZA200202106B (en) 2003-01-09
IL148624A0 (en) 2002-09-12
KR20020034188A (ko) 2002-05-08
NO20021254L (no) 2002-05-07
WO2001021662A1 (de) 2001-03-29
CN1269838C (zh) 2006-08-16
SK3622002A3 (en) 2003-06-03
EE05187B1 (et) 2009-06-15
HRP20020226B1 (en) 2010-08-31
RU2002110278A (ru) 2004-03-27
NO329723B1 (no) 2010-12-06
KR100737189B1 (ko) 2007-07-10
CA2385287C (en) 2010-10-19
DE50014189D1 (de) 2007-05-03
RS50435B (sr) 2009-12-31
RU2261867C2 (ru) 2005-10-10
SK287865B6 (sk) 2012-02-03
CN1550502A (zh) 2004-12-01
CN1374970A (zh) 2002-10-16
HUP0202596A2 (hu) 2002-12-28
TR200200667T2 (tr) 2002-06-21
IL148624A (en) 2009-08-03
HUP0202596A3 (en) 2005-01-28
HK1068352A1 (en) 2005-04-29
CZ2002899A3 (cs) 2002-06-12
CA2385287A1 (en) 2001-03-29
AU775923B2 (en) 2004-08-19
AR025660A1 (es) 2002-12-11
MEP27908A (en) 2010-10-10
YU14602A (sh) 2005-07-19
NZ517825A (en) 2004-07-30
PL205117B1 (pl) 2010-03-31
PL354444A1 (en) 2004-01-12
JP2003510041A (ja) 2003-03-18
ATE357458T1 (de) 2007-04-15
CN1192037C (zh) 2005-03-09
DK1216259T3 (da) 2007-07-02

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Legal Events

Date Code Title Description
B25D Requested change of name of applicant approved

Owner name: SANOFI-AVENTIS DEUTSCHLAND GMBH (DE)

Free format text: ALTERADO DE: AVENTIS PHARMA DEUTSCHLAND GMBH

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]