EE05187B1 - Hirudiini prekursor, selle kasutamine ja meetod Leu-hirudiini valmistamiseks - Google Patents

Hirudiini prekursor, selle kasutamine ja meetod Leu-hirudiini valmistamiseks

Info

Publication number
EE05187B1
EE05187B1 EEP200200139A EEP200200139A EE05187B1 EE 05187 B1 EE05187 B1 EE 05187B1 EE P200200139 A EEP200200139 A EE P200200139A EE P200200139 A EEP200200139 A EE P200200139A EE 05187 B1 EE05187 B1 EE 05187B1
Authority
EE
Estonia
Prior art keywords
hirudin
leu
preparation
precursor
hirudin precursor
Prior art date
Application number
EEP200200139A
Other languages
English (en)
Estonian (et)
Inventor
Habermann Paul
Bender Rudolf
Original Assignee
Aventis Pharma Deutschland Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Deutschland Gmbh filed Critical Aventis Pharma Deutschland Gmbh
Publication of EE200200139A publication Critical patent/EE200200139A/xx
Publication of EE05187B1 publication Critical patent/EE05187B1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EEP200200139A 1999-09-18 2000-09-01 Hirudiini prekursor, selle kasutamine ja meetod Leu-hirudiini valmistamiseks EE05187B1 (et)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19944870A DE19944870A1 (de) 1999-09-18 1999-09-18 Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
PCT/EP2000/008537 WO2001021662A1 (de) 1999-09-18 2000-09-01 Signalsequenzen zur herstellung von leu-hirudin über sekretion durch e. coli in das kulturmedium

Publications (2)

Publication Number Publication Date
EE200200139A EE200200139A (et) 2003-06-16
EE05187B1 true EE05187B1 (et) 2009-06-15

Family

ID=7922547

Family Applications (1)

Application Number Title Priority Date Filing Date
EEP200200139A EE05187B1 (et) 1999-09-18 2000-09-01 Hirudiini prekursor, selle kasutamine ja meetod Leu-hirudiini valmistamiseks

Country Status (29)

Country Link
US (1) US7455987B1 (xx)
EP (1) EP1216259B1 (xx)
JP (1) JP4669181B2 (xx)
KR (1) KR100737189B1 (xx)
CN (2) CN1269838C (xx)
AR (1) AR025660A1 (xx)
AT (1) ATE357458T1 (xx)
AU (1) AU775923B2 (xx)
BR (1) BR0014519A (xx)
CA (1) CA2385287C (xx)
CZ (1) CZ2002899A3 (xx)
DE (2) DE19944870A1 (xx)
DK (1) DK1216259T3 (xx)
EE (1) EE05187B1 (xx)
HK (2) HK1049172A1 (xx)
HR (1) HRP20020226B1 (xx)
HU (1) HUP0202596A3 (xx)
IL (2) IL148624A0 (xx)
ME (1) MEP27908A (xx)
MX (1) MXPA02002469A (xx)
NO (1) NO329723B1 (xx)
NZ (1) NZ517825A (xx)
PL (1) PL205117B1 (xx)
RS (1) RS50435B (xx)
RU (1) RU2261867C2 (xx)
SK (1) SK287865B6 (xx)
TR (2) TR200501541T2 (xx)
WO (1) WO2001021662A1 (xx)
ZA (1) ZA200202106B (xx)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
WO2005089093A2 (en) * 2003-11-21 2005-09-29 Dow Global Technologies Inc. Improved expression systems with sec-system secretion
EP2412816B1 (en) 2004-07-26 2014-12-03 Pfenex Inc. Process for improved protein expression by strain engineering
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
DE102006044841A1 (de) 2006-09-22 2008-04-03 Wacker Chemie Ag Signalpeptid zur Produktion von rekombinanten Proteinen
BRPI0807834A2 (pt) 2007-01-31 2014-07-22 Dow Global Technologies Inc " sequências-líder bacterianas para expressão aumentada ".
MX2009011523A (es) 2007-04-27 2009-11-09 Dow Global Technologies Inc Metodo para clasificar rapidamente huespedes microbianos para identificar ciertas cepas con rendimiento y/o calidad mejorados en la expresion de proteinas heterologas.
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
ES2853935T3 (es) 2013-03-12 2021-09-20 Massachusetts Gen Hospital Proteínas modificadas de sustancia inhibidora muleriana (MIS) y usos de las mismas para el tratamiento de enfermedades
CA3151082A1 (en) 2013-12-11 2015-06-18 The General Hospital Corporation Use of mullerian inhibiting substance (mis) proteins for contraception and ovarian reserve preservation
CN108220369B (zh) * 2017-12-04 2021-03-12 广东双骏生物科技有限公司 一种生产重组水蛭素的方法
CN108359689B (zh) * 2018-01-19 2021-03-23 华中农业大学 利用抗病基因识别无毒效应蛋白以鉴定具有分泌功能信号肽的方法
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
EP0324712B1 (de) * 1985-04-11 1993-04-07 Hoechst Aktiengesellschaft Hirudin-Derivat
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
AU604925B2 (en) * 1988-02-23 1991-01-03 Schering Aktiengesellschaft A hirudin derivative
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
JPH07119237B2 (ja) * 1990-11-08 1995-12-20 株式会社ジャパンエナジー ヒルジン変異体，その製造法及び抗凝血剤
ATE140929T1 (de) * 1990-11-08 1996-08-15 Japan Energy Corp Hirudinmutante, deren herstellung, antikoagulans, sekretorischer vektor, durch besagten vektor transformierter mikroorganismus und herstellung eines produkts durch besagten mikroorganismus
NZ243084A (en) * 1991-06-12 1995-08-28 Regeneron Pharma Production and recovery of recombinant neurotrophins, genes for a modified lamb signal sequence, fusion genes and plasmids
US5389529A (en) * 1991-06-12 1995-02-14 Regeneron Pharmaceuticals, Inc. Modified lamβ signal sequence and processes for producing recombinant neurotrophins
DE4140381A1 (de) 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet

Also Published As

Publication number Publication date
HRP20020226A2 (en) 2004-04-30
KR100737189B1 (ko) 2007-07-10
CN1269838C (zh) 2006-08-16
TR200200667T2 (tr) 2002-06-21
JP4669181B2 (ja) 2011-04-13
RU2002110278A (ru) 2004-03-27
NO20021254L (no) 2002-05-07
MXPA02002469A (es) 2002-08-28
BR0014519A (pt) 2002-06-11
IL148624A (en) 2009-08-03
JP2003510041A (ja) 2003-03-18
AU6842100A (en) 2001-04-24
ZA200202106B (en) 2003-01-09
US7455987B1 (en) 2008-11-25
KR20020034188A (ko) 2002-05-08
CN1374970A (zh) 2002-10-16
CN1192037C (zh) 2005-03-09
DK1216259T3 (da) 2007-07-02
HRP20020226B1 (en) 2010-08-31
EP1216259A1 (de) 2002-06-26
IL148624A0 (en) 2002-09-12
DE50014189D1 (de) 2007-05-03
ATE357458T1 (de) 2007-04-15
CA2385287C (en) 2010-10-19
CA2385287A1 (en) 2001-03-29
PL205117B1 (pl) 2010-03-31
HUP0202596A3 (en) 2005-01-28
HK1049172A1 (en) 2003-05-02
HUP0202596A2 (hu) 2002-12-28
YU14602A (sh) 2005-07-19
MEP27908A (en) 2010-10-10
HK1068352A1 (en) 2005-04-29
WO2001021662A1 (de) 2001-03-29
PL354444A1 (en) 2004-01-12
AU775923B2 (en) 2004-08-19
DE19944870A1 (de) 2001-03-29
RU2261867C2 (ru) 2005-10-10
SK287865B6 (sk) 2012-02-03
SK3622002A3 (en) 2003-06-03
NO20021254D0 (no) 2002-03-13
CZ2002899A3 (cs) 2002-06-12
RS50435B (sr) 2009-12-31
EP1216259B1 (de) 2007-03-21
NO329723B1 (no) 2010-12-06
EE200200139A (et) 2003-06-16
NZ517825A (en) 2004-07-30
AR025660A1 (es) 2002-12-11
TR200501541T2 (tr) 2005-06-21
CN1550502A (zh) 2004-12-01

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Legal Events

Date Code Title Description
KB4A Valid patent at the end of a year

Effective date: 20101231

MM4A Lapsed by not paying the annual fees

Effective date: 20120901