TR200200667T2 - Leu-hirudinin E.coli ile kültür ortamında ayrışarak üretilmesinin sinyal sekansları. - Google Patents

Leu-hirudinin E.coli ile kültür ortamında ayrışarak üretilmesinin sinyal sekansları.

Info

Publication number
TR200200667T2
TR200200667T2 TR2002/00667T TR200200667T TR200200667T2 TR 200200667 T2 TR200200667 T2 TR 200200667T2 TR 2002/00667 T TR2002/00667 T TR 2002/00667T TR 200200667 T TR200200667 T TR 200200667T TR 200200667 T2 TR200200667 T2 TR 200200667T2
Authority
TR
Turkey
Prior art keywords
coli
hirudin
production
leu
dissociation
Prior art date
Application number
TR2002/00667T
Other languages
English (en)
Inventor
Habermann Paul
Bender Rudolf
Original Assignee
Aventis Pharma Deutschland Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Deutschland Gmbh filed Critical Aventis Pharma Deutschland Gmbh
Publication of TR200200667T2 publication Critical patent/TR200200667T2/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Abstract

Karsisinda bulunulan bulus, bir sinyal sekansi ve Leu-Hirudin'in sekansini içeren bir hirudin öncülü, bunun üretimi ve kullanimi ve ayrica E.coli' de her hangi proteinlerin ifrazatli ifade edilmesi için sinyal sekanslarinin tespitinde uygulanan bir yöntem ve E.coli' de her hangi proteinlerin ifrazatli ifadesi için uygulanan yöntemler.
TR2002/00667T 1999-09-18 2000-09-01 Leu-hirudinin E.coli ile kültür ortamında ayrışarak üretilmesinin sinyal sekansları. TR200200667T2 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19944870A DE19944870A1 (de) 1999-09-18 1999-09-18 Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium

Publications (1)

Publication Number Publication Date
TR200200667T2 true TR200200667T2 (tr) 2002-06-21

Family

ID=7922547

Family Applications (2)

Application Number Title Priority Date Filing Date
TR2005/01541T TR200501541T2 (tr) 1999-09-18 2000-09-01 Leu-hirudinin e.coli ile kültür ortamında ayrışarak üretilmesinin sinyal sekansları
TR2002/00667T TR200200667T2 (tr) 1999-09-18 2000-09-01 Leu-hirudinin E.coli ile kültür ortamında ayrışarak üretilmesinin sinyal sekansları.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
TR2005/01541T TR200501541T2 (tr) 1999-09-18 2000-09-01 Leu-hirudinin e.coli ile kültür ortamında ayrışarak üretilmesinin sinyal sekansları

Country Status (29)

Country Link
US (1) US7455987B1 (tr)
EP (1) EP1216259B1 (tr)
JP (1) JP4669181B2 (tr)
KR (1) KR100737189B1 (tr)
CN (2) CN1192037C (tr)
AR (1) AR025660A1 (tr)
AT (1) ATE357458T1 (tr)
AU (1) AU775923B2 (tr)
BR (1) BR0014519A (tr)
CA (1) CA2385287C (tr)
CZ (1) CZ2002899A3 (tr)
DE (2) DE19944870A1 (tr)
DK (1) DK1216259T3 (tr)
EE (1) EE05187B1 (tr)
HK (2) HK1049172A1 (tr)
HR (1) HRP20020226B1 (tr)
HU (1) HUP0202596A3 (tr)
IL (2) IL148624A0 (tr)
ME (1) MEP27908A (tr)
MX (1) MXPA02002469A (tr)
NO (1) NO329723B1 (tr)
NZ (1) NZ517825A (tr)
PL (1) PL205117B1 (tr)
RS (1) RS50435B (tr)
RU (1) RU2261867C2 (tr)
SK (1) SK287865B6 (tr)
TR (2) TR200501541T2 (tr)
WO (1) WO2001021662A1 (tr)
ZA (1) ZA200202106B (tr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP2327718B1 (en) 2003-11-21 2016-03-23 Pfenex, Inc. Improved expression systems with SEC-system secretion
CA2574953A1 (en) 2004-07-26 2006-02-09 Dow Global Technolgies Inc. Process for improved protein expression by strain engineering
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
DE102006044841A1 (de) * 2006-09-22 2008-04-03 Wacker Chemie Ag Signalpeptid zur Produktion von rekombinanten Proteinen
US7618799B2 (en) 2007-01-31 2009-11-17 Dow Global Technologies Inc Bacterial leader sequences for increased expression
CN101688213A (zh) 2007-04-27 2010-03-31 陶氏环球技术公司 用于快速筛选微生物宿主以鉴定某些在表达异源蛋白质方面具有改善的产量和/或质量的菌株的方法
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
AU2014248768A1 (en) 2013-03-12 2015-09-24 Massachusetts Eye And Ear Infirmary Modified mullerian inhibiting substance (MIS) proteins and uses thereof for the treatment of diseases
ES2912283T3 (es) 2013-12-11 2022-05-25 Massachusetts Gen Hospital Uso de proteínas de tipo sustancia inhibidora mülleriana (SIM) para la anticoncepción y conservación de la reserva ovárica
CN108220369B (zh) * 2017-12-04 2021-03-12 广东双骏生物科技有限公司 一种生产重组水蛭素的方法
CN108359689B (zh) * 2018-01-19 2021-03-23 华中农业大学 利用抗病基因识别无毒效应蛋白以鉴定具有分泌功能信号肽的方法
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
FI96956C (fi) * 1985-04-11 1996-09-25 Hoechst Ag Menetelmä terapeuttisesti käyttökelpoisen hirudiinijohdannaisen valmistamiseksi
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
AU604925B2 (en) * 1988-02-23 1991-01-03 Schering Aktiengesellschaft A hirudin derivative
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
DK0511393T3 (da) * 1990-11-08 1996-12-09 Japan Energy Corp Hirudin-mutant, fremstilling af denne, antikoagulant, sekretorisk vektor, mikroorganisme transformeret af vektoren og fremstilling af produkt ud fra mikroorganismen
JPH07119237B2 (ja) * 1990-11-08 1995-12-20 株式会社ジャパンエナジー ヒルジン変異体，その製造法及び抗凝血剤
NZ243084A (en) * 1991-06-12 1995-08-28 Regeneron Pharma Production and recovery of recombinant neurotrophins, genes for a modified lamb signal sequence, fusion genes and plasmids
US5389529A (en) * 1991-06-12 1995-02-14 Regeneron Pharmaceuticals, Inc. Modified lamβ signal sequence and processes for producing recombinant neurotrophins
DE4140381A1 (de) 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet

Also Published As

Publication number Publication date
YU14602A (sh) 2005-07-19
NO20021254D0 (no) 2002-03-13
PL354444A1 (en) 2004-01-12
CN1269838C (zh) 2006-08-16
IL148624A (en) 2009-08-03
HUP0202596A3 (en) 2005-01-28
CN1192037C (zh) 2005-03-09
AU775923B2 (en) 2004-08-19
ATE357458T1 (de) 2007-04-15
HRP20020226A2 (en) 2004-04-30
CN1550502A (zh) 2004-12-01
EE05187B1 (et) 2009-06-15
MXPA02002469A (es) 2002-08-28
EE200200139A (et) 2003-06-16
EP1216259B1 (de) 2007-03-21
JP4669181B2 (ja) 2011-04-13
AR025660A1 (es) 2002-12-11
RS50435B (sr) 2009-12-31
MEP27908A (en) 2010-10-10
NZ517825A (en) 2004-07-30
RU2261867C2 (ru) 2005-10-10
SK287865B6 (sk) 2012-02-03
US7455987B1 (en) 2008-11-25
WO2001021662A1 (de) 2001-03-29
ZA200202106B (en) 2003-01-09
HK1068352A1 (en) 2005-04-29
CN1374970A (zh) 2002-10-16
HK1049172A1 (en) 2003-05-02
PL205117B1 (pl) 2010-03-31
DE50014189D1 (de) 2007-05-03
DK1216259T3 (da) 2007-07-02
KR20020034188A (ko) 2002-05-08
HRP20020226B1 (en) 2010-08-31
DE19944870A1 (de) 2001-03-29
CA2385287A1 (en) 2001-03-29
CZ2002899A3 (cs) 2002-06-12
KR100737189B1 (ko) 2007-07-10
BR0014519A (pt) 2002-06-11
EP1216259A1 (de) 2002-06-26
IL148624A0 (en) 2002-09-12
NO20021254L (no) 2002-05-07
NO329723B1 (no) 2010-12-06
SK3622002A3 (en) 2003-06-03
AU6842100A (en) 2001-04-24
JP2003510041A (ja) 2003-03-18
RU2002110278A (ru) 2004-03-27
TR200501541T2 (tr) 2005-06-21
HUP0202596A2 (hu) 2002-12-28
CA2385287C (en) 2010-10-19

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