ES8802261A1 - Un metodo de detectar en una muestra un microorganismo o fragmento del mismo que tiene una porcion molecularmente re-conocible - Google Patents
Un metodo de detectar en una muestra un microorganismo o fragmento del mismo que tiene una porcion molecularmente re-conocibleInfo
- Publication number
- ES8802261A1 ES8802261A1 ES547003A ES547003A ES8802261A1 ES 8802261 A1 ES8802261 A1 ES 8802261A1 ES 547003 A ES547003 A ES 547003A ES 547003 A ES547003 A ES 547003A ES 8802261 A1 ES8802261 A1 ES 8802261A1
- Authority
- ES
- Spain
- Prior art keywords
- entity
- polynucleotide
- polynucleotide sequences
- assay method
- analyte
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
METODO DE DETECCION EN UNA MUESTRA DE UN ANALITO (A) QUE TIENE UNA PORCION MOLECULARMENTE RECONOCIBLE EN SI MISMO, QUE COMPRENDE LAS OPERACIONES DE: A) FORMAR UN COMPLEJO QUE COMPRENDE UNA ENTIDAD FORMADORA DE PUENTES MOLECULARES; B) QUE TIENE EN SI MISMO: I) UNA PORCION CAPAZ DE RECONOCER DICHA PORCION MOLECULARMENTE RECONOCIBLE EN DICHO ANALITO, E II) UNA PORCION QUE COMPRENDE UNA SECUENCIA DE POLINUCLEOTIDO; Y UNA ENTIDAD DE SEÑALIZACION C) QUE TIENE EN SIMISMA: I) UNA PORCION DE POLINUCLEOTIDO CAPAZ DE REANILLARSE A DICHA PORCION DE POLINUCLEOTIDO DE LA ENTIDAD FORMADORA DE PUENTE, PARA FORMAR UN HIBRIDO ESTABLE, E II) UNA PORCION GENERADORA DE SEÑAL. FINALMENTE SE DETECTA LA SEÑAL CON AYUDA DE MEDIOS CONOCIDOS PARA LA DETECCION: MARCADOR RADIACTIVO, BACTERIANO, FLUORESCENTE, UN ANTICUERPO, UNA PARTICULA DE LATEX, FERRITINA, ETC.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US49192983A | 1983-05-05 | 1983-05-05 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8802261A1 true ES8802261A1 (es) | 1988-04-16 |
ES547003A0 ES547003A0 (es) | 1988-04-16 |
Family
ID=23954256
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES532182A Expired ES8608169A1 (es) | 1983-05-05 | 1984-05-04 | Un metodo de detectar en una muestra un analito |
ES547003A Expired ES8802261A1 (es) | 1983-05-05 | 1985-09-16 | Un metodo de detectar en una muestra un microorganismo o fragmento del mismo que tiene una porcion molecularmente re-conocible |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES532182A Expired ES8608169A1 (es) | 1983-05-05 | 1984-05-04 | Un metodo de detectar en una muestra un analito |
Country Status (11)
Country | Link |
---|---|
US (1) | US9134302B2 (es) |
EP (2) | EP0611828B1 (es) |
JP (1) | JP2577881B2 (es) |
AT (2) | ATE125947T1 (es) |
AU (1) | AU583787B2 (es) |
CA (1) | CA1228811A (es) |
DE (2) | DE3486400T2 (es) |
DK (1) | DK223384A (es) |
ES (2) | ES8608169A1 (es) |
IL (1) | IL71666A (es) |
NO (1) | NO165975C (es) |
Families Citing this family (61)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1338856C (en) * | 1983-07-05 | 1997-01-21 | Jannis Stavrianopoulos | In vivo labelling of polynucleotide sequences |
US4707440A (en) * | 1984-01-30 | 1987-11-17 | Enzo Biochem, Inc. | Nucleic acid hybridization assay and detectable molecules useful in such assay |
AU3844485A (en) * | 1984-02-09 | 1985-08-15 | Enzo Biochem Inc. | Heterologous detection of cabeled dna |
GB8405437D0 (en) * | 1984-03-01 | 1984-04-04 | Amersham Int Plc | Detecting polynucleotide sequences |
CA1253777A (en) * | 1984-06-01 | 1989-05-09 | Robert J. Carrico | Nucleic acid hybridization assay employing immobilized rna probes |
FI72146C (fi) * | 1985-01-02 | 1987-04-13 | Orion Yhtymae Oy | Foerfarande foer identifiering av nukleinsyror. |
US4824775A (en) * | 1985-01-03 | 1989-04-25 | Molecular Diagnostics, Inc. | Cells labeled with multiple Fluorophores bound to a nucleic acid carrier |
GB8509367D0 (en) * | 1985-04-12 | 1985-05-15 | Amersham Int Plc | Nucleic acid hybridisation |
DE3680156D1 (de) * | 1985-05-31 | 1991-08-14 | Amoco Corp | Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen. |
ES8707343A1 (es) * | 1985-06-13 | 1987-07-16 | Amgen | Un metodo para aislar una secuencia de acido nucleico objetivo seleccionada |
US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
US4882269A (en) * | 1985-12-13 | 1989-11-21 | Princeton University | Amplified hybridization assay |
US4822731A (en) * | 1986-01-09 | 1989-04-18 | Cetus Corporation | Process for labeling single-stranded nucleic acids and hybridizaiton probes |
US4900659A (en) * | 1986-01-30 | 1990-02-13 | Enzo Biochem, Inc. | Nucleotide sequence composition and method for detection of Neisseria gonorrhoeae and method for screening for a nucleotide sequence that is specific for a genetically distinct group |
US4839293A (en) * | 1986-02-24 | 1989-06-13 | The Trustees Of Columbia University In The City Of New York | DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino acid sequences present in streptavidin and uses thereof |
US4925785A (en) * | 1986-03-07 | 1990-05-15 | Biotechnica Diagnostics, Inc. | Nucleic acid hybridization assays |
US7138516B1 (en) | 1986-11-24 | 2006-11-21 | Gen-Probe Incorporated | Oligonucleotide probes for the detection and/or quantitation of non-viral organisms |
US7087742B1 (en) | 1986-11-24 | 2006-08-08 | Gen-Probe Incorporated | Oligonucleotide probes for the detection and/or quantitation of non-viral organisms |
JP2565552B2 (ja) * | 1987-10-15 | 1996-12-18 | カイロン コーポレイション | 核酸マルチマーおよびそれを用いた増幅核酸ハイブリダイゼーション分析法 |
FR2644163B2 (fr) * | 1988-07-13 | 1994-09-30 | Bioprobe Systems Sa | Procede de marquage d'une sonde nucleique et trousse de reactifs pour la mise en oeuvre de ce procede |
US7172863B1 (en) | 1988-12-09 | 2007-02-06 | Gen-Probe Incorporated | Nucleic acid probes and methods for detecting Neisseria gonorrhoeae |
US5683874A (en) * | 1991-03-27 | 1997-11-04 | Research Corporation Technologies, Inc. | Single-stranded circular oligonucleotides capable of forming a triplex with a target sequence |
AU661490B2 (en) * | 1991-03-27 | 1995-07-27 | Research Corporation Technologies, Inc. | Single-stranded circular oligonucleotides |
US5329461A (en) * | 1992-07-23 | 1994-07-12 | Acrogen, Inc. | Digital analyte detection system |
US7713528B1 (en) | 1993-02-18 | 2010-05-11 | Enzo Therapeutics, Inc. | Method for in vivo delivery of active compounds using reagent conjugate |
CA2140081C (en) | 1994-01-13 | 2008-04-01 | Dean L. Engelhardt | Process, construct and conjugate for producing multiple nucleic acid copies |
US6986985B1 (en) | 1994-01-13 | 2006-01-17 | Enzo Life Sciences, Inc. | Process for producing multiple nucleic acid copies in vivo using a protein-nucleic acid construct |
WO1997019193A2 (en) | 1995-11-21 | 1997-05-29 | Yale University | Unimolecular segment amplification and detection |
US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
DE69719057T2 (de) * | 1996-07-12 | 2003-12-04 | Tm Technologies Inc., Woburn | Verfahren zur signalverstärkung |
US8703480B1 (en) | 1997-03-21 | 2014-04-22 | Enzo Therapeutics, Inc. | Biological function effecting viral vectors and chimeric cells useful as packaging cell lines and target cells |
EP1005561A4 (en) | 1997-03-21 | 2004-03-31 | Enzo Therapeutics Inc A Fully | VECTOR, VIRAL VECTORS AND PACKAGING CELL LINES TO THEIR REPRODUCTION |
US6124120A (en) * | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
US6316229B1 (en) | 1998-07-20 | 2001-11-13 | Yale University | Single molecule analysis target-mediated ligation of bipartite primers |
US6255082B1 (en) | 1998-09-15 | 2001-07-03 | Yale University | Artificial long terminal repeat vectors |
AU770993B2 (en) * | 1998-09-15 | 2004-03-11 | Yale University | Molecular cloning using rolling circle amplification |
US6613516B1 (en) | 1999-10-30 | 2003-09-02 | Affymetrix, Inc. | Preparation of nucleic acid samples |
AU2001271722B2 (en) | 2000-06-30 | 2006-04-13 | Qiagen, Gmbh | Signal amplification with lollipop probes |
US20040161741A1 (en) | 2001-06-30 | 2004-08-19 | Elazar Rabani | Novel compositions and processes for analyte detection, quantification and amplification |
US20030096275A1 (en) * | 2001-08-20 | 2003-05-22 | Laing Lance G. | Biosensor for small molecule analytes |
US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
US9156986B2 (en) | 2003-04-03 | 2015-10-13 | Enzo Life Sciences, Inc. | Multisignal labeling reagents and processes and uses therefor |
US9696298B2 (en) | 2003-04-03 | 2017-07-04 | Enzo Life Sciences, Inc. | Multisignal reagents for labeling analytes |
US7514551B2 (en) * | 2003-04-03 | 2009-04-07 | Enzo Life Sciences, Inc. | Multisignal labeling reagents, and processes and uses therefor |
EP1863908B1 (de) | 2005-04-01 | 2010-11-17 | Qiagen GmbH | Reverse transkription und amplifikation von rna bei simultaner degradierung von dna |
EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
WO2007044671A2 (en) | 2005-10-06 | 2007-04-19 | Lucigen Corporation | Thermostable viral polymerases and methods of use |
US20090162845A1 (en) | 2007-12-20 | 2009-06-25 | Elazar Rabbani | Affinity tag nucleic acid and protein compositions, and processes for using same |
EP4108782B1 (en) | 2011-12-22 | 2023-06-07 | President and Fellows of Harvard College | Compositions and methods for analyte detection |
US9347938B2 (en) | 2012-03-09 | 2016-05-24 | Invisible Sentinel, Inc. | Methods for detecting multiple analytes with a single signal |
US9347948B2 (en) * | 2012-06-04 | 2016-05-24 | The Scripps Research Institute | Phenyl glyoxal probes |
US10060910B2 (en) | 2012-07-24 | 2018-08-28 | Enzo Life Sciences, Inc. | Multisignal reagents for labeling analytes |
US9617607B2 (en) | 2013-01-08 | 2017-04-11 | Enzo Biochem, Inc. | Diagnosis and treatment of viral diseases |
WO2014110127A1 (en) | 2013-01-08 | 2014-07-17 | Enzo Biochem, Inc. | Diagnosis and treatment of viral diseases |
KR102368591B1 (ko) | 2013-04-30 | 2022-02-25 | 캘리포니아 인스티튜트 오브 테크놀로지 | 순차적 하이브리드화 바코딩에 의한 분자의 멀티플렉스 표지화 |
EP3754028A1 (en) | 2019-06-18 | 2020-12-23 | Apollo Life Sciences GmbH | Method of signal encoding of analytes in a sample |
CN115698322A (zh) | 2020-06-18 | 2023-02-03 | 解析生物科学有限公司 | 用于检测样品中的不同分析物的多重方法 |
WO2023012272A1 (en) | 2021-08-06 | 2023-02-09 | Resolve Biosciences Gmbh | Method for detecting an analyte in a pathogen-comprising sample |
JP2024537737A (ja) | 2021-10-25 | 2024-10-16 | リゾルブ バイオサイエンシズ ゲゼルシャフト ミット ベシュレンクテル ハフツング | 生体試料を表面上にマウントする方法 |
WO2024002726A1 (en) | 2022-06-30 | 2024-01-04 | Resolve Biosciences Gmbh | High resolution multiplex method for detecting at least two targets with a distance of beyond the diffraction limit in a sample |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3755086A (en) | 1971-02-09 | 1973-08-28 | Hoffmann La Roche | Diagnostic method utilizing synthetic deoxyrilionucleotide oligomer template |
US3798131A (en) | 1972-04-24 | 1974-03-19 | Pasadena Foundation For Medica | Assay for polymeric dna as a method for detecting malignancy |
FR2422956A1 (fr) | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
US4302204A (en) | 1979-07-02 | 1981-11-24 | The Board Of Trustees Of Leland Stanford Junior University | Transfer and detection of nucleic acids |
US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
US4711955A (en) | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
CA1219824A (en) * | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
CA1190838A (en) | 1981-07-17 | 1985-07-23 | Cavit Akin | Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer |
CA1180647A (en) | 1981-07-17 | 1985-01-08 | Cavit Akin | Light-emitting polynucleotide hybridization diagnostic method |
FI63596C (fi) | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
AU1606083A (en) * | 1982-05-07 | 1983-12-02 | Paragon Diagnostics | Competitive immunofluorescence assay and test kit |
US5241060A (en) | 1982-06-23 | 1993-08-31 | Enzo Diagnostics, Inc. | Base moiety-labeled detectable nucleatide |
US5260433A (en) | 1982-06-23 | 1993-11-09 | Enzo Diagnostics, Inc. | Saccharide specific binding system labeled nucleotides |
CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
US4556643A (en) | 1982-07-26 | 1985-12-03 | Agracetus | Assay method and probe for polynucleotide sequences |
GB8306426D0 (en) | 1983-03-09 | 1983-04-13 | Malcolm A D B | Detecting polynucleotide sequence |
-
1984
- 1984-03-30 CA CA000451043A patent/CA1228811A/en not_active Expired
- 1984-04-18 NO NO841581A patent/NO165975C/no unknown
- 1984-04-27 IL IL71666A patent/IL71666A/xx not_active IP Right Cessation
- 1984-05-04 EP EP94102973A patent/EP0611828B1/en not_active Expired - Lifetime
- 1984-05-04 DE DE3486400T patent/DE3486400T2/de not_active Expired - Lifetime
- 1984-05-04 EP EP84105028A patent/EP0128332B1/en not_active Expired - Lifetime
- 1984-05-04 AT AT84105028T patent/ATE125947T1/de not_active IP Right Cessation
- 1984-05-04 DK DK223384A patent/DK223384A/da not_active Application Discontinuation
- 1984-05-04 ES ES532182A patent/ES8608169A1/es not_active Expired
- 1984-05-04 AU AU27675/84A patent/AU583787B2/en not_active Ceased
- 1984-05-04 AT AT94102973T patent/ATE197480T1/de active
- 1984-05-04 DE DE3486478T patent/DE3486478T2/de not_active Expired - Lifetime
- 1984-05-04 JP JP59090041A patent/JP2577881B2/ja not_active Expired - Lifetime
-
1985
- 1985-09-16 ES ES547003A patent/ES8802261A1/es not_active Expired
-
1995
- 1995-06-07 US US08/479,995 patent/US9134302B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
ES8608169A1 (es) | 1986-06-01 |
NO165975C (no) | 1991-05-08 |
JP2577881B2 (ja) | 1997-02-05 |
DE3486400T2 (de) | 1996-01-04 |
IL71666A0 (en) | 1984-07-31 |
US9134302B2 (en) | 2015-09-15 |
IL71666A (en) | 1989-02-28 |
NO841581L (no) | 1984-11-06 |
AU583787B2 (en) | 1989-05-11 |
DE3486400D1 (de) | 1995-09-07 |
US20100273145A1 (en) | 2010-10-28 |
ATE197480T1 (de) | 2000-11-11 |
EP0611828A1 (en) | 1994-08-24 |
DK223384D0 (da) | 1984-05-04 |
ES532182A0 (es) | 1986-06-01 |
CA1228811A (en) | 1987-11-03 |
DE3486478D1 (de) | 2000-12-14 |
EP0128332A1 (en) | 1984-12-19 |
JPS59220647A (ja) | 1984-12-12 |
EP0128332B1 (en) | 1995-08-02 |
EP0611828B1 (en) | 2000-11-08 |
ES547003A0 (es) | 1988-04-16 |
ATE125947T1 (de) | 1995-08-15 |
NO165975B (no) | 1991-01-28 |
DE3486478T2 (de) | 2001-06-28 |
DK223384A (da) | 1984-11-06 |
AU2767584A (en) | 1984-11-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD2A | Announcement of lapse in spain |
Effective date: 20151230 |