DE3680156D1 - Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen. - Google Patents

Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen.

Info

Publication number
DE3680156D1
DE3680156D1 DE8686304069T DE3680156T DE3680156D1 DE 3680156 D1 DE3680156 D1 DE 3680156D1 DE 8686304069 T DE8686304069 T DE 8686304069T DE 3680156 T DE3680156 T DE 3680156T DE 3680156 D1 DE3680156 D1 DE 3680156D1
Authority
DE
Germany
Prior art keywords
strand
reinforcing
complementary dna
label
hybridization signals
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686304069T
Other languages
English (en)
Inventor
Mark Leo Collins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP Corp North America Inc
Original Assignee
BP Corp North America Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BP Corp North America Inc filed Critical BP Corp North America Inc
Application granted granted Critical
Publication of DE3680156D1 publication Critical patent/DE3680156D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
DE8686304069T 1985-05-31 1986-05-29 Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen. Expired - Fee Related DE3680156D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US73993785A 1985-05-31 1985-05-31

Publications (1)

Publication Number Publication Date
DE3680156D1 true DE3680156D1 (de) 1991-08-14

Family

ID=24974398

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686304069T Expired - Fee Related DE3680156D1 (de) 1985-05-31 1986-05-29 Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen.

Country Status (4)

Country Link
EP (1) EP0204510B1 (de)
JP (1) JPS61293399A (de)
AT (1) ATE65092T1 (de)
DE (1) DE3680156D1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
FR2619819B1 (fr) * 1987-08-27 1990-08-10 Agronomique Inst Nat Rech Sondes moleculaires d'adn specifique du genome male de ruminants, notamment de la sous-famille des bovines et en particulier du genre bos, sequences flanquantes pour l'amplification de ces sondes et applications desdites sondes
US5328827A (en) * 1987-08-27 1994-07-12 Institut National De La Recherche Agronomique - Inra Specific DNA molecular probes for Bos-type male genome
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5359100A (en) * 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same
JPH01219563A (ja) * 1988-02-27 1989-09-01 Doujin Kagaku Kenkyusho:Kk 化学発光の写真法による検出方法
NO165894C (no) * 1988-05-24 1991-04-24 Gunnar Paulsen Analysemetode for gener.
DE3829535A1 (de) * 1988-08-31 1990-03-15 Biotest Ag Nukleinsaeure-fragmente aus eukaryontischen genomen, verfahren zu ihrer herstellung und ihre verwendung zum nachweis von restriktions-fragment-laengen-polymorphismen (rflps)
SE462497B (sv) * 1988-11-30 1990-07-02 Clas Fredrik Runesson Kaelland Saett foer att aktivitetsbestaemma polymeraser
US5629153A (en) * 1990-01-10 1997-05-13 Chiron Corporation Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
CA2039517C (en) * 1990-04-03 2006-11-07 David Segev Dna probe signal amplification
JPH04110661A (ja) * 1990-08-30 1992-04-13 Tosoh Corp 核酸の免疫学的測定方法
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
CA2126952C (en) * 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
US20030104361A1 (en) 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5830658A (en) * 1995-05-31 1998-11-03 Lynx Therapeutics, Inc. Convergent synthesis of branched and multiply connected macromolecular structures
FR2746413B1 (fr) * 1996-03-19 1998-04-24 Bio Merieux Detection d'une sequence nucleotidique avec amplification de signal
EP0938588B1 (de) * 1996-07-12 2003-02-12 TM Technologies Inc. Verfahren zur signalverstärkung
DE07008633T1 (de) 1997-12-11 2008-12-18 Merial Virus des multisystemischen Kümmersyndroms bei Absatzferkeln
EP1816200B1 (de) 1997-12-11 2016-03-09 Merial Virus des multisystemischen Kümmersyndroms bei Absatzferkeln
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US7118864B2 (en) 2001-06-15 2006-10-10 Quiatech Ab Amplifiable probe

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8306426D0 (en) * 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
CA1228811A (en) * 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
US4626501A (en) * 1983-09-02 1986-12-02 Integrated Genetics, Inc. Labeled DNA
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
DE3420925A1 (de) * 1984-06-05 1985-12-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zum nachweis einer nukleinsaeure-sequenz

Also Published As

Publication number Publication date
EP0204510A3 (en) 1987-01-28
EP0204510B1 (de) 1991-07-10
EP0204510A2 (de) 1986-12-10
ATE65092T1 (de) 1991-07-15
JPS61293399A (ja) 1986-12-24

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee