ATE65092T1 - Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen. - Google Patents

Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen.

Info

Publication number
ATE65092T1
ATE65092T1 AT86304069T AT86304069T ATE65092T1 AT E65092 T1 ATE65092 T1 AT E65092T1 AT 86304069 T AT86304069 T AT 86304069T AT 86304069 T AT86304069 T AT 86304069T AT E65092 T1 ATE65092 T1 AT E65092T1
Authority
AT
Austria
Prior art keywords
strand
amplification
label
complementary dna
dna strands
Prior art date
Application number
AT86304069T
Other languages
English (en)
Inventor
Mark Leo Collins
Original Assignee
Amoco Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amoco Corp filed Critical Amoco Corp
Application granted granted Critical
Publication of ATE65092T1 publication Critical patent/ATE65092T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT86304069T 1985-05-31 1986-05-29 Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen. ATE65092T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73993785A 1985-05-31 1985-05-31
EP86304069A EP0204510B1 (de) 1985-05-31 1986-05-29 Verstärkung von Hybridisierungssignalen durch Verwendung von komplementarischen DNS-Strängen

Publications (1)

Publication Number Publication Date
ATE65092T1 true ATE65092T1 (de) 1991-07-15

Family

ID=24974398

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86304069T ATE65092T1 (de) 1985-05-31 1986-05-29 Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen.

Country Status (4)

Country Link
EP (1) EP0204510B1 (de)
JP (1) JPS61293399A (de)
AT (1) ATE65092T1 (de)
DE (1) DE3680156D1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
US5328827A (en) * 1987-08-27 1994-07-12 Institut National De La Recherche Agronomique - Inra Specific DNA molecular probes for Bos-type male genome
FR2619819B1 (fr) * 1987-08-27 1990-08-10 Agronomique Inst Nat Rech Sondes moleculaires d'adn specifique du genome male de ruminants, notamment de la sous-famille des bovines et en particulier du genre bos, sequences flanquantes pour l'amplification de ces sondes et applications desdites sondes
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5359100A (en) * 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
JPH01219563A (ja) * 1988-02-27 1989-09-01 Doujin Kagaku Kenkyusho:Kk 化学発光の写真法による検出方法
NO165894C (no) * 1988-05-24 1991-04-24 Gunnar Paulsen Analysemetode for gener.
DE3829535A1 (de) * 1988-08-31 1990-03-15 Biotest Ag Nukleinsaeure-fragmente aus eukaryontischen genomen, verfahren zu ihrer herstellung und ihre verwendung zum nachweis von restriktions-fragment-laengen-polymorphismen (rflps)
SE462497B (sv) * 1988-11-30 1990-07-02 Clas Fredrik Runesson Kaelland Saett foer att aktivitetsbestaemma polymeraser
US5629153A (en) * 1990-01-10 1997-05-13 Chiron Corporation Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
CA2039517C (en) * 1990-04-03 2006-11-07 David Segev Dna probe signal amplification
JPH04110661A (ja) * 1990-08-30 1992-04-13 Tosoh Corp 核酸の免疫学的測定方法
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
CA2126952C (en) * 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
US20030104361A1 (en) 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5830658A (en) * 1995-05-31 1998-11-03 Lynx Therapeutics, Inc. Convergent synthesis of branched and multiply connected macromolecular structures
FR2746413B1 (fr) * 1996-03-19 1998-04-24 Bio Merieux Detection d'une sequence nucleotidique avec amplification de signal
ATE232559T1 (de) 1996-07-12 2003-02-15 Tm Technologies Inc Verfahren zur signalverstärkung
JP3795751B2 (ja) 1997-12-11 2006-07-12 ユニバーシティ オブ サスカッチェワン ブタからの離乳後多全身系消耗症候群ウイルス
EP1816200B1 (de) 1997-12-11 2016-03-09 Merial Virus des multisystemischen Kümmersyndroms bei Absatzferkeln
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
WO2002103052A1 (en) 2001-06-15 2002-12-27 Quiatech Ab Amplifiable probe

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8306426D0 (en) * 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
CA1228811A (en) * 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
US4626501A (en) * 1983-09-02 1986-12-02 Integrated Genetics, Inc. Labeled DNA
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
DE3420925A1 (de) * 1984-06-05 1985-12-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zum nachweis einer nukleinsaeure-sequenz

Also Published As

Publication number Publication date
JPS61293399A (ja) 1986-12-24
EP0204510B1 (de) 1991-07-10
EP0204510A3 (en) 1987-01-28
DE3680156D1 (de) 1991-08-14
EP0204510A2 (de) 1986-12-10

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Legal Events

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RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties