ATE65092T1 - Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen. - Google Patents

Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen.

Info

Publication number
ATE65092T1
ATE65092T1 AT86304069T AT86304069T ATE65092T1 AT E65092 T1 ATE65092 T1 AT E65092T1 AT 86304069 T AT86304069 T AT 86304069T AT 86304069 T AT86304069 T AT 86304069T AT E65092 T1 ATE65092 T1 AT E65092T1
Authority
AT
Austria
Prior art keywords
strand
amplification
label
complementary dna
dna strands
Prior art date
Application number
AT86304069T
Other languages
English (en)
Inventor
Mark Leo Collins
Original Assignee
Amoco Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amoco Corp filed Critical Amoco Corp
Application granted granted Critical
Publication of ATE65092T1 publication Critical patent/ATE65092T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT86304069T 1985-05-31 1986-05-29 Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen. ATE65092T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73993785A 1985-05-31 1985-05-31
EP86304069A EP0204510B1 (de) 1985-05-31 1986-05-29 Verstärkung von Hybridisierungssignalen durch Verwendung von komplementarischen DNS-Strängen

Publications (1)

Publication Number Publication Date
ATE65092T1 true ATE65092T1 (de) 1991-07-15

Family

ID=24974398

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86304069T ATE65092T1 (de) 1985-05-31 1986-05-29 Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen.

Country Status (4)

Country Link
EP (1) EP0204510B1 (de)
JP (1) JPS61293399A (de)
AT (1) ATE65092T1 (de)
DE (1) DE3680156D1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
FR2619819B1 (fr) * 1987-08-27 1990-08-10 Agronomique Inst Nat Rech Sondes moleculaires d'adn specifique du genome male de ruminants, notamment de la sous-famille des bovines et en particulier du genre bos, sequences flanquantes pour l'amplification de ces sondes et applications desdites sondes
US5328827A (en) * 1987-08-27 1994-07-12 Institut National De La Recherche Agronomique - Inra Specific DNA molecular probes for Bos-type male genome
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5359100A (en) * 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
JPH01219563A (ja) * 1988-02-27 1989-09-01 Doujin Kagaku Kenkyusho:Kk 化学発光の写真法による検出方法
NO165894C (no) * 1988-05-24 1991-04-24 Gunnar Paulsen Analysemetode for gener.
DE3829535A1 (de) * 1988-08-31 1990-03-15 Biotest Ag Nukleinsaeure-fragmente aus eukaryontischen genomen, verfahren zu ihrer herstellung und ihre verwendung zum nachweis von restriktions-fragment-laengen-polymorphismen (rflps)
SE462497B (sv) * 1988-11-30 1990-07-02 Clas Fredrik Runesson Kaelland Saett foer att aktivitetsbestaemma polymeraser
US5629153A (en) * 1990-01-10 1997-05-13 Chiron Corporation Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
CA2039517C (en) * 1990-04-03 2006-11-07 David Segev Dna probe signal amplification
JPH04110661A (ja) * 1990-08-30 1992-04-13 Tosoh Corp 核酸の免疫学的測定方法
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
CA2126952C (en) * 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
US20030104361A1 (en) 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5830658A (en) * 1995-05-31 1998-11-03 Lynx Therapeutics, Inc. Convergent synthesis of branched and multiply connected macromolecular structures
FR2746413B1 (fr) * 1996-03-19 1998-04-24 Bio Merieux Detection d'une sequence nucleotidique avec amplification de signal
WO1998002580A2 (en) * 1996-07-12 1998-01-22 Tm Technologies, Inc. Signal amplification method
EP1816200B1 (de) 1997-12-11 2016-03-09 Merial Virus des multisystemischen Kümmersyndroms bei Absatzferkeln
DE07008633T1 (de) 1997-12-11 2008-12-18 Merial Virus des multisystemischen Kümmersyndroms bei Absatzferkeln
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
WO2002103052A1 (en) 2001-06-15 2002-12-27 Quiatech Ab Amplifiable probe

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8306426D0 (en) * 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
CA1228811A (en) * 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
US4626501A (en) * 1983-09-02 1986-12-02 Integrated Genetics, Inc. Labeled DNA
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
DE3420925A1 (de) * 1984-06-05 1985-12-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zum nachweis einer nukleinsaeure-sequenz

Also Published As

Publication number Publication date
DE3680156D1 (de) 1991-08-14
EP0204510A3 (en) 1987-01-28
EP0204510B1 (de) 1991-07-10
EP0204510A2 (de) 1986-12-10
JPS61293399A (ja) 1986-12-24

Similar Documents

Publication Publication Date Title
ATE65092T1 (de) Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen.
NO852022L (no) Nukleinsyre-hybridiseringsanalyse ved anvendelse av detekterbare anti-hybrid-antistoffer.
ATE187820T1 (de) Verfahren zur analyse partikelverstärkter agglutinationsreaktionen auf zentrifugalanalysatoren durch bestimmung der trübungsaufhellung
ATE253645T1 (de) Verfahren zur bestimmung von säugetier nukleinsäuren aus stuhlproben und dafür benötigte reagenzien
FI844865A7 (fi) Nukleiinihappo-osoitin, tutkimusmenetelmäja reagenssijärjestelmä polynukleoditijakson havaitsemiseksi sekä siihkäytettävä vasta-aine.
DK463089D0 (da) Fremgangsmaade og reagenssaet til detektion af en specifik nucleinsyresekvens i en proeve indeholdende en nucleinsyre
WO1999064867A8 (en) Multiple assay method
ATE93896T1 (de) Nachweis und bestimmung der menge an rns und dns.
ATE66695T1 (de) Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.
ATE452206T1 (de) Verfahren für verbesserte lumineszenztests unter verwendung von mikropartikeln
FI950487L (fi) Menetelmä, reagenssi ja testipakkaus nukleiinihapposekvenssien totamiseksi ja monistamiseksi
ATE12010T1 (de) Verfahren zur bestimmung von antigen-antikoerper- reaktionen und reagens hierfuer.
ATE86670T1 (de) Verwendung von oxidase-enzymsystemen in chemilumineszierenden tests.
ATE133257T1 (de) Ermittlung und quantifizierung mehrerer analyten unter verwendung von markierungstechniken
FI902934A0 (fi) Foerfarande foer bestaemning av nukleinsyra.
DE3689557D1 (de) Verfahren zur immunologischen bestimmung einer biologischen substanz in einer probe.
DE68907305D1 (de) Methode zur analyse einer spezifischen sequenz von dns oder rns, reagenzien und kits dafuer.
DE3875184D1 (de) System und verfahren fuer eine visuelle probe einer zu bestimmenden substanz.
DE3587845D1 (de) Verfahren zur Bestimmung eines Liganden.
DK0916951T3 (da) Fremgangsmåde til kalibrering af et indholdsbestemmelsessystem af typen ligand-anti-ligand

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties