BRPI0706801B8 - método de purificação de cardiomiócitos ou cardiomiócitos programados derivado de células-tronco ou fetos - Google Patents
método de purificação de cardiomiócitos ou cardiomiócitos programados derivado de células-tronco ou fetosInfo
- Publication number
- BRPI0706801B8 BRPI0706801B8 BRPI0706801A BRPI0706801A BRPI0706801B8 BR PI0706801 B8 BRPI0706801 B8 BR PI0706801B8 BR PI0706801 A BRPI0706801 A BR PI0706801A BR PI0706801 A BRPI0706801 A BR PI0706801A BR PI0706801 B8 BRPI0706801 B8 BR PI0706801B8
- Authority
- BR
- Brazil
- Prior art keywords
- cardiomyocytes
- condition
- stem cells
- fetuses
- derived
- Prior art date
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
- C12N2500/14—Calcium; Ca chelators; Calcitonin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/44—Thiols, e.g. mercaptoethanol
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/99—Serum-free medium
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Rheumatology (AREA)
- Cardiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
método de purificação de cardiomiõcitos ou cardiomiócitos programados derivado de células- tronco ou fetos a presente invenção refere-se ao desenvolvimento de um método para purificar cardiomiácitos com alto grau de purificação e com alto rendimento a partir de uma mistura celular compreendendo cardiomiócitos derivados de fetos e de células-tronco usando várias características as quais não foram anteriormente esperadas para serem usadas para a purificação de cardiomiócitos, ou as quais são recentemente descobertas, em que o referido método é executado sem sofrer qualquer modificação genética ou sem adicionar quaisquer proteínas ou agentes biologicamente ativos especiais. os inventores da presente invenção descobriram que os cardio-miócitos foram altamente selecionados e purificados de forma eficaz pelo cultivo de cardiomiácitos derivados de células-tronco embrionárias no meio de cultura sob uma condição selecionada de uma condição selecionada com pouco soro, uma condição suplementada com pouca glicose, uma condição nutricional baixa, uma condição com pouco cálcio, uma condição com ph brandamente ácido, uma condição suplementada com ácido lático, uma condição suplementada com ácido aspártico/ácido glutâmico e/ou uma condição suplementada com ácido pirúvico. os inventores da presente invenção ainda descobriram que o método inventado acima, em relação às células-tronco embrionárias, foi aplicável para selecionar e purificar cardiomiácitos derivados de fetos ou de células-tronco adultas.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006023770 | 2006-01-31 | ||
| JP2006-023770 | 2006-01-31 | ||
| PCT/JP2007/051563 WO2007088874A1 (ja) | 2006-01-31 | 2007-01-31 | 幹細胞及び胎児由来の心筋細胞及び予定心筋細胞の精製方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BRPI0706801A2 BRPI0706801A2 (pt) | 2011-04-05 |
| BRPI0706801B1 BRPI0706801B1 (pt) | 2020-04-07 |
| BRPI0706801B8 true BRPI0706801B8 (pt) | 2021-05-25 |
Family
ID=38327445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0706801A BRPI0706801B8 (pt) | 2006-01-31 | 2007-01-31 | método de purificação de cardiomiócitos ou cardiomiócitos programados derivado de células-tronco ou fetos |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9115342B2 (pt) |
| EP (2) | EP2468851A1 (pt) |
| JP (2) | JP5312804B2 (pt) |
| KR (2) | KR101455144B1 (pt) |
| CN (2) | CN103409368B (pt) |
| AU (1) | AU2007210580B2 (pt) |
| BR (1) | BRPI0706801B8 (pt) |
| CA (1) | CA2640644C (pt) |
| IL (1) | IL193095A (pt) |
| RU (1) | RU2426784C2 (pt) |
| SG (1) | SG169359A1 (pt) |
| WO (1) | WO2007088874A1 (pt) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2006286149B2 (en) | 2005-08-29 | 2012-09-13 | Technion Research And Development Foundation Ltd. | Media for culturing stem cells |
| KR101455144B1 (ko) * | 2006-01-31 | 2014-10-27 | 다이이찌 산쿄 가부시키가이샤 | 줄기세포 및 태아에서 유래된 심근세포 및 예정 심근세포의 제조방법 |
| EP2059586B1 (en) | 2006-08-02 | 2016-07-20 | Technion Research & Development Foundation Ltd. | Methods of expanding embryonic stem cells in a suspension culture |
| EP2415862B1 (en) * | 2009-03-30 | 2015-05-06 | Daiichi Sankyo Company, Limited | Method for inducing cell death in pluripotent stem cells and differentiated cells other than cardiac myocytes |
| CA3174901A1 (en) | 2009-11-12 | 2011-05-19 | Technion Research & Development Foundation Ltd. | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| AU2011268056B2 (en) * | 2010-06-18 | 2014-04-24 | Cellular Dynamics International, Inc. | Cardiomyocyte medium with dialyzed serum |
| KR101364965B1 (ko) * | 2010-10-06 | 2014-02-18 | (주)차바이오앤디오스텍 | 인간 배아줄기세포 유래 심근세포의 배양 및 정제 방법 |
| JP5840855B2 (ja) | 2011-03-30 | 2016-01-06 | 学校法人東京女子医科大学 | 胚性幹細胞から心筋シートを製造する方法 |
| JP2014521337A (ja) * | 2011-07-29 | 2014-08-28 | セルラー ダイナミクス インターナショナル， インコーポレイテッド | 幹細胞由来の組織細胞中の代謝成熟 |
| ES2523903B1 (es) * | 2013-04-30 | 2015-09-09 | Fundación Pública Andaluza Progreso Y Salud | Método de obtención de datos útiles para el diagnóstico diferencial de la fibrosis hepática |
| CN105039399A (zh) * | 2014-04-23 | 2015-11-11 | 复旦大学 | 多能干细胞-遗传性心肌病心肌细胞及其制备方法 |
| SG11201700023WA (en) * | 2014-07-16 | 2017-02-27 | Heartseed Inc | New undifferentiated stem cell removal and myocardial purification and refinement culture medium |
| EP3196297B1 (en) | 2014-09-16 | 2020-11-04 | Osaka University | Method for producing cardiomyocyte population from pluripotent stem cells |
| JP6938154B2 (ja) * | 2014-11-07 | 2021-09-22 | 国立大学法人大阪大学 | 未分化細胞が除去された分化誘導細胞集団、その利用及びその製造方法 |
| JP6622007B2 (ja) * | 2015-05-29 | 2019-12-18 | オリンパス株式会社 | 細胞評価方法 |
| JP7089484B2 (ja) | 2016-06-01 | 2022-06-22 | ミルテニイ ビオテック ベー．ファー． ウント コー．カーゲー | ヒト多能性幹細胞由来の心筋細胞および心筋細胞サブポピュレーションの生成、同定ならびに単離のための方法 |
| CN106244527B (zh) * | 2016-08-29 | 2019-10-08 | 广东依浦赛生物科技有限公司 | 人源iPS干细胞体外定向分化为心肌细胞的试剂盒及方法 |
| US20190300857A1 (en) * | 2016-10-17 | 2019-10-03 | Keio University | Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells |
| SG11201805456WA (en) | 2016-11-01 | 2019-05-30 | Terumo Corp | Modification method for sheet-shaped cell culture |
| CN106754663A (zh) * | 2016-11-30 | 2017-05-31 | 中国人民解放军第三军医大学第三附属医院 | 促进成年哺乳动物心肌细胞存活的体外培养方法及监测成年哺乳动物心肌细胞增殖的方法 |
| JP2018093823A (ja) | 2016-12-15 | 2018-06-21 | Ｈｅａｒｔｓｅｅｄ株式会社 | 未分化幹細胞除去剤及び未分化幹細胞除去方法 |
| JP7298844B2 (ja) | 2017-06-05 | 2023-06-27 | テルモ株式会社 | 細胞培養物の製造方法 |
| CN107828721A (zh) * | 2017-11-10 | 2018-03-23 | 山东农业大学 | 一种鸡胚心肌细胞的培养及免疫组织化学鉴定方法 |
| EP3739040B1 (en) | 2018-09-27 | 2023-07-05 | Osaka University | Sheeting method for pluripotent stem cell-derived cells |
| JPWO2020067436A1 (ja) * | 2018-09-27 | 2021-08-30 | 国立大学法人大阪大学 | 多能性幹細胞由来細胞の移植片形成方法 |
| WO2020067435A1 (ja) * | 2018-09-27 | 2020-04-02 | 国立大学法人大阪大学 | 多能性幹細胞由来細胞のシート化方法 |
| CN110904024A (zh) * | 2019-11-13 | 2020-03-24 | 武汉济源高科技有限公司 | 一种悬浮细胞培养中去除死细胞的方法 |
| CN111621470B (zh) * | 2020-06-08 | 2023-06-27 | 广东源心再生医学有限公司 | 一种高效低毒的心肌纯化培养基及方法 |
| CN114591895A (zh) * | 2022-04-12 | 2022-06-07 | 澳门大学 | 一种诱导干细胞分化为心肌细胞的方法及其培养基组合 |
| WO2024008773A1 (en) | 2022-07-05 | 2024-01-11 | Repairon Gmbh | Differentiation of ipscs in bioreactors |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL47012C (pt) | 1933-03-23 | |||
| GB9903804D0 (en) | 1999-02-20 | 1999-04-14 | Univ Sheffield | Pluripotential cells-2 |
| CA2395950A1 (en) | 1999-12-28 | 2001-07-05 | Kyowa Hakko Kogyo Co., Ltd. | Cells capable of differentiating into heart muscle cells |
| EP1395652A2 (en) | 2000-11-30 | 2004-03-10 | Stemron, Inc. | Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same |
| US7732199B2 (en) * | 2001-07-12 | 2010-06-08 | Geron Corporation | Process for making transplantable cardiomyocytes from human embryonic stem cells |
| CN1543500B (zh) * | 2001-07-12 | 2014-04-09 | 杰龙公司 | 从人多能干细胞产生心肌细胞系细胞 |
| AU2005224670B2 (en) * | 2004-03-19 | 2010-11-11 | Asterias Biotherapeutics, Inc. | Method for making high purity cardiomyocyte preparations suitable for regenerative medicine |
| GB2429718B (en) * | 2004-06-01 | 2008-12-17 | Es Cell Int Pte Ltd | Improved cardiomyocyte differentiation |
| US20080254003A1 (en) | 2004-12-22 | 2008-10-16 | Robert Passier | Differentiation of Human Embryonic Stem Cells and Cardiomyocytes and Cardiomyocyte Progenitors Derived Therefrom |
| KR101455144B1 (ko) * | 2006-01-31 | 2014-10-27 | 다이이찌 산쿄 가부시키가이샤 | 줄기세포 및 태아에서 유래된 심근세포 및 예정 심근세포의 제조방법 |
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2007
- 2007-01-31 KR KR20127027002A patent/KR101455144B1/ko active IP Right Grant
- 2007-01-31 CA CA2640644A patent/CA2640644C/en active Active
- 2007-01-31 SG SG201100658-2A patent/SG169359A1/en unknown
- 2007-01-31 CN CN201310348017.1A patent/CN103409368B/zh active Active
- 2007-01-31 AU AU2007210580A patent/AU2007210580B2/en active Active
- 2007-01-31 EP EP20120160317 patent/EP2468851A1/en not_active Withdrawn
- 2007-01-31 BR BRPI0706801A patent/BRPI0706801B8/pt active IP Right Grant
- 2007-01-31 WO PCT/JP2007/051563 patent/WO2007088874A1/ja active Application Filing
- 2007-01-31 JP JP2007556880A patent/JP5312804B2/ja active Active
- 2007-01-31 KR KR1020087020152A patent/KR101348325B1/ko active IP Right Grant
- 2007-01-31 RU RU2008135313A patent/RU2426784C2/ru active
- 2007-01-31 US US12/162,684 patent/US9115342B2/en active Active
- 2007-01-31 CN CNA200780003957XA patent/CN101374942A/zh active Pending
- 2007-01-31 EP EP07713734.7A patent/EP1983042B1/en active Active
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2008
- 2008-07-28 IL IL193095A patent/IL193095A/en active IP Right Grant
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2013
- 2013-04-30 JP JP2013095524A patent/JP5756145B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103409368A (zh) | 2013-11-27 |
| JP5756145B2 (ja) | 2015-07-29 |
| IL193095A0 (en) | 2009-02-11 |
| CA2640644A1 (en) | 2007-08-09 |
| US9115342B2 (en) | 2015-08-25 |
| EP1983042B1 (en) | 2017-03-15 |
| KR20080097197A (ko) | 2008-11-04 |
| RU2008135313A (ru) | 2010-03-20 |
| KR20120123608A (ko) | 2012-11-08 |
| AU2007210580B2 (en) | 2012-10-11 |
| CN101374942A (zh) | 2009-02-25 |
| JP2013143968A (ja) | 2013-07-25 |
| US20090275132A1 (en) | 2009-11-05 |
| WO2007088874A1 (ja) | 2007-08-09 |
| KR101348325B1 (ko) | 2014-01-08 |
| EP2468851A1 (en) | 2012-06-27 |
| SG169359A1 (en) | 2011-03-30 |
| AU2007210580A1 (en) | 2007-08-09 |
| EP1983042A1 (en) | 2008-10-22 |
| IL193095A (en) | 2015-06-30 |
| JPWO2007088874A1 (ja) | 2009-06-25 |
| KR101455144B1 (ko) | 2014-10-27 |
| BRPI0706801B1 (pt) | 2020-04-07 |
| EP1983042A4 (en) | 2009-06-17 |
| JP5312804B2 (ja) | 2013-10-09 |
| BRPI0706801A2 (pt) | 2011-04-05 |
| CA2640644C (en) | 2013-11-26 |
| RU2426784C2 (ru) | 2011-08-20 |
| CN103409368B (zh) | 2016-05-25 |
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