BRPI0511482A - incorporação sìtio-especìfica de aminoácidos artificiais contendo átomos pesados em proteìnas para determinação de estrutura cristalina - Google Patents

incorporação sìtio-especìfica de aminoácidos artificiais contendo átomos pesados em proteìnas para determinação de estrutura cristalina

Info

Publication number
BRPI0511482A
BRPI0511482A BRPI0511482-9A BRPI0511482A BRPI0511482A BR PI0511482 A BRPI0511482 A BR PI0511482A BR PI0511482 A BRPI0511482 A BR PI0511482A BR PI0511482 A BRPI0511482 A BR PI0511482A
Authority
BR
Brazil
Prior art keywords
amino acids
proteins
specific incorporation
determination
acids containing
Prior art date
Application number
BRPI0511482-9A
Other languages
English (en)
Inventor
Jianming Xie
Lei Wang
Ning Wu
Peter G Schultz
Glen Spraggon
Original Assignee
Scripps Research Inst
Irm Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst, Irm Llc filed Critical Scripps Research Inst
Publication of BRPI0511482A publication Critical patent/BRPI0511482A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

INCORPORAçãO SìTIO-ESPECìFICA DE AMINOáCIDOS ARTIFICIAIS CONTENDO áTOMOS PESADOS EM PROTEìNAS PARA DETERMINAçãO DE ESTRUTURA CRISTALINA. São providos sistemas de tradução e outras composições incluindo aminocil tRNA-sintetase ortogonais que carregam preferencialmente um tRNA ortogonal com um aminoácido iodado ou bromado. ácidos nucléicos codificando tais sintetases também são descritos, como o são métodos e kits para produzir proteínas incluindo aminoácidos contendo átomo pesado, p. ex., aminoácidos bromados ou iodados. Também são descritas métodos para determinar a estrutura de uma proteína, p. ex., uma proteína em que um átomo pesado tenha sido incorporado sítio-especificamente através do uso de um par ortogonal de tRNA/aminoacil tRNA-sintetase.
BRPI0511482-9A 2004-05-25 2005-05-24 incorporação sìtio-especìfica de aminoácidos artificiais contendo átomos pesados em proteìnas para determinação de estrutura cristalina BRPI0511482A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US57455404P 2004-05-25 2004-05-25
US60204804P 2004-08-16 2004-08-16
PCT/US2005/018168 WO2005116237A2 (en) 2004-05-25 2005-05-24 Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination

Publications (1)

Publication Number Publication Date
BRPI0511482A true BRPI0511482A (pt) 2007-12-26

Family

ID=35451472

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0511482-9A BRPI0511482A (pt) 2004-05-25 2005-05-24 incorporação sìtio-especìfica de aminoácidos artificiais contendo átomos pesados em proteìnas para determinação de estrutura cristalina

Country Status (9)

Country Link
US (1) US7399619B2 (pt)
EP (1) EP1774018A2 (pt)
JP (1) JP2008500050A (pt)
AU (1) AU2005248392A1 (pt)
BR (1) BRPI0511482A (pt)
CA (1) CA2567624A1 (pt)
IL (1) IL179211A0 (pt)
MX (1) MXPA06013586A (pt)
WO (1) WO2005116237A2 (pt)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2444098C (en) * 2001-04-19 2016-06-21 The Scripps Research Institute Methods and composition for the production of orthogonal trna-aminoacyltrna synthetase pairs
WO2004035605A2 (en) * 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
ES2301099T3 (es) * 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
EP1794312A4 (en) * 2004-09-22 2009-08-12 Scripps Research Inst LOCAL LABELING OF PROTEINS FOR NMR STUDIES
MX2007004892A (es) * 2004-10-27 2007-08-16 Scripps Research Inst Componentes de traduccion ortogonales para la incorporacion in vivo de aminoacidos no naturales.
US8586340B2 (en) * 2005-10-12 2013-11-19 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
WO2007092475A2 (en) 2006-02-06 2007-08-16 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
US7960141B2 (en) * 2006-03-09 2011-06-14 The Scripps Research Institute Systems for the expression of orthogonal translation components in eubacterial host cells
RU2467069C2 (ru) * 2006-03-09 2012-11-20 Зе Скрипс Ресеч Инститьют Система экспрессии компонентов ортогональной трансляции в эубактериальной клетке-хозяине
JP5249194B2 (ja) 2006-03-16 2013-07-31 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現
CA2652894A1 (en) * 2006-05-23 2007-12-06 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
RU2009110152A (ru) 2006-09-21 2010-10-27 Зе Скрипс Ресеч Инститьют (Us) Генетически закодированная формула селективно-сульфатных белков в эубактерии
BRPI0718389A2 (pt) 2006-10-18 2012-04-10 Scripps Research Inst incorporação genética de aminoácidos antinaturais em proteìnas em células de mamìferos
CA2703736A1 (en) 2007-10-25 2009-04-30 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
WO2009061369A2 (en) 2007-11-02 2009-05-14 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
EP2220218A4 (en) * 2007-11-02 2010-12-08 Scripps Research Inst BORONIC AMINO ACID GENETICALLY CODED
CN101939410A (zh) * 2007-12-11 2011-01-05 斯克利普斯研究院 甲基营养酵母菌巴斯德毕赤酵母中的体内非天然氨基酸表达
ES2531290T3 (es) 2008-01-03 2015-03-12 Basf Enzymes Llc Transferasas y oxidorreductasas, ácidos nucleicos que las codifican y métodos para prepararlas y usarlas
JP2011514322A (ja) * 2008-02-08 2011-05-06 ザ スクリプス リサーチ インスティチュート 遺伝的にコードされる非天然アミノ酸による免疫寛容解除
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
US8609383B2 (en) 2008-12-10 2013-12-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2444098C (en) 2001-04-19 2016-06-21 The Scripps Research Institute Methods and composition for the production of orthogonal trna-aminoacyltrna synthetase pairs
WO2004035605A2 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
SI1578949T1 (sl) 2002-10-16 2009-08-31 Scripps Research Inst Usmerjena vključitev keto aminokislin v proteine
CA2508939A1 (en) 2002-12-22 2004-07-15 The Scripps Research Institute Protein arrays
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP2392923B1 (en) 2003-06-18 2014-05-21 The Scripps Research Institute Method of producing proteins comprising unnatural amino acid
EP1658366A4 (en) 2003-07-07 2006-08-09 Scripps Research Inst COMPOSITIONS OF ORTHOGONAL GLUTAMYL-TRNA AND AMINOACYL-TRNA-SYNTHETASEPAARES AND THEIR USES
ES2301099T3 (es) 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
PT1687401E (pt) 2003-10-14 2013-02-26 Scripps Research Inst Incorporação de aminoácidos activos em oxidação-redução específica do local em proteínas
US7709604B2 (en) 2003-12-18 2010-05-04 The Scripps Research Institute Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Also Published As

Publication number Publication date
IL179211A0 (en) 2007-03-08
CA2567624A1 (en) 2005-12-08
WO2005116237A2 (en) 2005-12-08
US7399619B2 (en) 2008-07-15
AU2005248392A1 (en) 2005-12-08
MXPA06013586A (es) 2007-11-09
WO2005116237A3 (en) 2007-04-19
EP1774018A2 (en) 2007-04-18
JP2008500050A (ja) 2008-01-10
US20050272121A1 (en) 2005-12-08

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 5A, 6A E 7A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2158 DE 15/05/2012.