SG10201701224UA - Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode - Google Patents

Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode

Info

Publication number
SG10201701224UA
SG10201701224UA SG10201701224UA SG10201701224UA SG10201701224UA SG 10201701224U A SG10201701224U A SG 10201701224UA SG 10201701224U A SG10201701224U A SG 10201701224UA SG 10201701224U A SG10201701224U A SG 10201701224UA SG 10201701224U A SG10201701224U A SG 10201701224UA
Authority
SG
Singapore
Prior art keywords
removal
protein aggregates
flowthrough mode
biopharmaceutical preparations
biopharmaceutical
Prior art date
Application number
SG10201701224UA
Inventor
Mikhail Kozlov
William Cataldo
Ajish Potty
Kevin Galipeau
James Hamzik
Lars Peeck
Joaquin Umana
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of SG10201701224UA publication Critical patent/SG10201701224UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/58—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing oxygen in addition to the carbonamido oxygen, e.g. N-methylolacrylamide, N-(meth)acryloylmorpholine
    • C08F220/585—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing oxygen in addition to the carbonamido oxygen, e.g. N-methylolacrylamide, N-(meth)acryloylmorpholine and containing other heteroatoms, e.g. 2-acrylamido-2-methylpropane sulfonic acid [AMPS]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/08—Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/16—Organic material
    • B01J39/18—Macromolecular compounds
    • B01J39/20—Macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/02—Column or bed processes
    • B01J47/026—Column or bed processes using columns or beds of different ion exchange materials in series
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/56—Acrylamide; Methacrylamide
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
    • C08F222/36—Amides or imides
    • C08F222/38—Amides
    • C08F222/385—Monomers containing two or more (meth)acrylamide groups, e.g. N,N'-methylenebisacrylamide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
SG10201701224UA 2012-03-12 2013-01-09 Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode SG10201701224UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261609533P 2012-03-12 2012-03-12
US201261666578P 2012-06-29 2012-06-29

Publications (1)

Publication Number Publication Date
SG10201701224UA true SG10201701224UA (en) 2017-04-27

Family

ID=48044702

Family Applications (1)

Application Number Title Priority Date Filing Date
SG10201701224UA SG10201701224UA (en) 2012-03-12 2013-01-09 Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode

Country Status (10)

Country Link
US (2) US20130245139A1 (en)
EP (3) EP3730510A1 (en)
JP (3) JP5768070B2 (en)
KR (4) KR101520753B1 (en)
CN (3) CN104817611B (en)
ES (2) ES2644744T3 (en)
PL (1) PL2639239T3 (en)
SG (1) SG10201701224UA (en)
SI (1) SI2639239T1 (en)
WO (1) WO2013138098A1 (en)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101871683B1 (en) 2010-07-30 2018-06-27 이엠디 밀리포어 코포레이션 Chromatogrphy media and method
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
WO2013028330A2 (en) * 2011-08-19 2013-02-28 Emd Millipore Corporation Methods of reducing level of one of more impurities in a sample during protein purification
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
EP2682168A1 (en) * 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
PL2812091T3 (en) 2012-09-17 2021-07-19 W.R. Grace & Co. - Conn. Chromatography media and devices
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
EP3395423B1 (en) * 2013-03-14 2023-09-20 Amgen Inc. Removal of leaked affinity purification ligand
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
CN105980047B (en) * 2013-12-12 2019-01-04 Emd密理博公司 Use the filter protein isolate containing acrylamide
PL3094390T3 (en) 2014-01-16 2021-12-06 W.R. Grace & Co. - Conn. Affinity chromatography media and chromatography devices
EP3100050B1 (en) * 2014-01-31 2019-08-28 Enginzyme AB Immobilized proteins and use thereof
US11389783B2 (en) 2014-05-02 2022-07-19 W.R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
WO2016013609A1 (en) * 2014-07-25 2016-01-28 旭化成メディカル株式会社 Carrier for positive-ion-exchange chromatography and method for using same
SG11201700030UA (en) * 2014-09-02 2017-02-27 Emd Millipore Corp High surface area fiber media with nano-fibrillated surface features
WO2016093251A1 (en) * 2014-12-08 2016-06-16 旭化成メディカル株式会社 Method for purifying physiologically active substance
WO2016093926A1 (en) 2014-12-08 2016-06-16 Emd Millipore Corporation Mixed bed ion exchange adsorber
JP2016210705A (en) * 2015-04-30 2016-12-15 昭和電工株式会社 Method of removing protein aggregates
KR102566292B1 (en) 2015-06-05 2023-08-10 더블유.알. 그레이스 앤드 캄파니-콘. Adsorbent bioprocessing clarifiers and methods of making and using the same
AU2016355739A1 (en) * 2015-11-18 2018-07-05 Merck Patent Gmbh Improved protein separation in ion exchange chromatography
CN106807330A (en) * 2015-11-27 2017-06-09 中国科学院大连化学物理研究所 The preparation and sorbing material and application of a kind of ordered structure sorbing material
JP7032046B2 (en) * 2016-01-22 2022-03-08 旭化成メディカル株式会社 Continuous constant flow rate purification method for bioactive substances
WO2017126496A1 (en) * 2016-01-22 2017-07-27 旭化成メディカル株式会社 Protein purification method
JP6621176B2 (en) * 2016-01-22 2019-12-18 旭化成メディカル株式会社 Protein purification method
EP3275897A1 (en) * 2016-07-27 2018-01-31 Biotest AG Process for preparing immunoglobulin compositions
EP3508493A4 (en) * 2016-08-31 2020-05-27 Kyowa Kirin Co., Ltd. Protein purification method using activated carbon
EP3509729A1 (en) 2016-09-09 2019-07-17 3M Innovative Properties Company Functionalized copolymers and use thereof
CN109689674A (en) * 2016-09-09 2019-04-26 3M创新有限公司 From the method for the monomeric protein separation collectin matter in biological solution
US20190217285A1 (en) 2016-09-09 2019-07-18 Asahi Kasei Medical Co., Ltd. Strong cation exchange chromatographic matrix and method for using same
KR20190076046A (en) * 2016-11-10 2019-07-01 산도즈 아게 Desalting process of protein solution
WO2019169040A1 (en) 2018-02-27 2019-09-06 Life Technologies Corporation Flocculant functionalized separation media
EP3762120A4 (en) * 2018-03-08 2021-09-01 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resin
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
CA3095850A1 (en) * 2018-04-03 2019-10-10 Merck Patent Gmbh Cex chromatography media and low salt elution of target proteins from biopharmaceutical feeds
US11045773B2 (en) * 2018-08-31 2021-06-29 Pall Corporation Salt tolerant porous medium
DE102018009597A1 (en) * 2018-12-07 2020-06-10 Sartorius Stedim Biotech Gmbh Device and method for multiple changes in the composition of a fluid
CN113302197A (en) * 2019-01-23 2021-08-24 第一三共株式会社 Method for purifying antibody including process using activated carbon material
US10981950B2 (en) * 2019-01-31 2021-04-20 Joanna Pezzini Therapeutic protein flow kit for a continuous purification system
CN114040815A (en) 2019-09-05 2022-02-11 生物辐射实验室股份有限公司 Anion exchange-hydrophobic mixed mode chromatography resin
KR20220101168A (en) 2019-11-22 2022-07-19 모르포시스 아게 Methods for Increasing Yield of Antibodies During Ion Exchange Chromatography
CN115397834A (en) * 2020-04-13 2022-11-25 3M创新有限公司 Flow-through method and apparatus for purifying target molecules
WO2021232009A1 (en) * 2020-05-15 2021-11-18 Stevenson Gary L Multistage oil reclamation system
CN116802206A (en) * 2020-12-28 2023-09-22 等离子体技术有限责任公司 Immunoglobulin G separation systems and methods for production scale
CN113416235B (en) * 2021-06-24 2023-04-25 苏州赛分科技股份有限公司 Liquid chromatography for purifying and separating virus antigens
CN113522256B (en) * 2021-07-19 2022-06-21 中国科学院兰州化学物理研究所 Preparation and application of hydrogel @ silicon dioxide liquid chromatography filler
KR20240046733A (en) 2021-08-23 2024-04-09 제넨테크, 인크. Perfusion Cation Exchange Chromatography Purification Process for Antibody Drug Conjugates

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4618533A (en) 1984-11-30 1986-10-21 Millipore Corporation Porous membrane having hydrophilic surface and process
US4944879A (en) 1989-07-27 1990-07-31 Millipore Corporation Membrane having hydrophilic surface
US6451260B1 (en) * 1997-08-26 2002-09-17 Dyax Corp. Method for producing microporous elements, the microporous elements thus produced and uses thereof
EP1500661A1 (en) 1998-06-01 2005-01-26 Genetech, Inc. Separation of protein monomers from aggregates by use of ion-exchange chromatography
US6783937B1 (en) * 1999-02-25 2004-08-31 Pall Corporation Negatively charged membrane
US6783713B2 (en) * 1999-05-03 2004-08-31 The Procter & Gamble Co. Filters for removal of pathogens from liquids
AUPQ389599A0 (en) * 1999-11-05 1999-12-02 Ilion Technology Corporation Polyelectrolyte gel
JP4918952B2 (en) * 2001-03-28 2012-04-18 Ｄｉｃ株式会社 Water-absorbing material
US7318972B2 (en) * 2001-09-07 2008-01-15 Itm Power Ltd. Hydrophilic polymers and their use in electrochemical cells
AU2003212912A1 (en) 2002-02-04 2003-09-02 Millipore Corporation Process for removing protein aggregates and virus from a protein solution
EP2143481A1 (en) * 2003-02-19 2010-01-13 Natrix Separations Inc. Composite materials comprising supported porous gels
WO2004087761A1 (en) * 2003-03-31 2004-10-14 Kirin Beer Kabushiki Kaisha Purification of human monoclonal antibody and human polyclonal antibody
WO2005042569A1 (en) 2003-10-24 2005-05-12 Amgen, Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
CN102276717B (en) * 2003-10-27 2015-09-09 惠氏公司 Use hydroxyapatite removing high molecular weight aggregates
SE0302911D0 (en) * 2003-10-31 2003-10-31 Amersham Biosciences Ab Novel separation matrix
KR101243601B1 (en) * 2004-10-21 2013-03-20 지이 헬스케어 바이오-사이언시스 에이비 Chromatography ligand
US20070012345A1 (en) * 2005-01-24 2007-01-18 Owens Tony L Lower limb-support ambulatory device
WO2006099308A2 (en) 2005-03-11 2006-09-21 Wyeth A method of weak partitioning chromatography
JP4976391B2 (en) * 2005-07-28 2012-07-18 バイオ−ラド ラボラトリーズ インコーポレイテッド Isolation of proteins based on isoelectric point using solid buffer
US7674836B2 (en) * 2006-07-28 2010-03-09 3M Innovative Properties Company Method of making macroporous cation exchange resins
PT2061803T (en) * 2006-08-28 2019-11-21 Ares Trading Sa Process for the purification of fc-containing proteins
DE102006061327A1 (en) * 2006-12-22 2008-06-26 Basf Construction Polymers Gmbh Grafting copolymer, useful e.g. as additives in construction chemical applications, water retention agents and fluid loss additives, comprises silica, which is reacted with an unsaturated silane and a polymer containing sulfonic acid
WO2008085116A1 (en) * 2007-01-10 2008-07-17 Ge Healthcare Bio-Sciences Ab Mult i -modal ion exchange chromotography resins
DE102007012786A1 (en) * 2007-03-16 2009-01-08 Construction Research & Technology Gmbh Preparation of sulfo-containing copolymers
US20080257814A1 (en) 2007-04-23 2008-10-23 Millipore Corporation Filtration Cartridge
EP2152405B2 (en) * 2007-05-25 2017-04-05 Merck Patent GmbH Graft copolymer for cation-exchange chromatography
EP2152745B2 (en) * 2007-06-01 2023-03-15 F. Hoffmann-La Roche AG Immunoglobulin purification
JP4897087B2 (en) 2007-10-03 2012-03-14 ミリポア・コーポレイション Laminated plate type filtration cartridge
JP5132278B2 (en) * 2007-11-28 2013-01-30 一般財団法人川村理化学研究所 Method for producing organic-inorganic composite hydrogel
WO2009105191A2 (en) 2008-02-19 2009-08-27 Bloom Energy Corporation Fuel cell system containing anode tail gas oxidizer and hybrid heat exchanger/reformer
EP2153877A1 (en) * 2008-07-30 2010-02-17 MERCK PATENT GmbH Mixed graft polymers for ion exchange chromatography
AU2008221604B2 (en) * 2008-09-22 2010-04-22 Commonwealth Scientific And Industrial Research Organisation Temperature-responsive polymer particles in protein separation applications
CN104801204B (en) * 2009-02-27 2019-01-01 Emd密理博公司 For removing the film with sulfonic acid group of protein aggregate
AU2010251271B2 (en) * 2009-05-20 2015-01-22 Basf Se Hydrophobically associating copolymers
EP2462157B1 (en) * 2009-08-07 2020-06-17 EMD Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
US20110301333A1 (en) * 2010-06-08 2011-12-08 Millipore Corporation Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts
GB201012603D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Protein purification
WO2012015379A1 (en) * 2010-07-29 2012-02-02 Millipore Corporation Grafting method to improve chromatography media performance
KR101871683B1 (en) * 2010-07-30 2018-06-27 이엠디 밀리포어 코포레이션 Chromatogrphy media and method

Also Published As

Publication number Publication date
KR20150093642A (en) 2015-08-18
SI2639239T1 (en) 2017-12-29
US20130245139A1 (en) 2013-09-19
WO2013138098A1 (en) 2013-09-19
CN103382215A (en) 2013-11-06
JP6231027B2 (en) 2017-11-15
JP2018048162A (en) 2018-03-29
KR101520753B1 (en) 2015-05-15
EP3312190B1 (en) 2020-05-27
ES2644744T3 (en) 2017-11-30
CN110256628A (en) 2019-09-20
KR101601812B1 (en) 2016-03-10
US20180215786A1 (en) 2018-08-02
KR20150043271A (en) 2015-04-22
EP3312190A1 (en) 2018-04-25
JP6580650B2 (en) 2019-09-25
EP2639239B1 (en) 2017-09-06
ES2812648T3 (en) 2021-03-17
CN104817611A (en) 2015-08-05
JP2013189427A (en) 2013-09-26
CN104817611B (en) 2019-06-18
JP2015110786A (en) 2015-06-18
KR101966692B1 (en) 2019-04-09
EP2639239A2 (en) 2013-09-18
PL2639239T3 (en) 2018-03-30
JP5768070B2 (en) 2015-08-26
EP3730510A1 (en) 2020-10-28
KR20130105340A (en) 2013-09-25
CN103382215B (en) 2016-08-24
KR20140141562A (en) 2014-12-10
EP2639239A3 (en) 2013-11-20

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