ATE425989T1 - Proteinreinigungsverfahren - Google Patents

Proteinreinigungsverfahren

Info

Publication number
ATE425989T1
ATE425989T1 AT02794380T AT02794380T ATE425989T1 AT E425989 T1 ATE425989 T1 AT E425989T1 AT 02794380 T AT02794380 T AT 02794380T AT 02794380 T AT02794380 T AT 02794380T AT E425989 T1 ATE425989 T1 AT E425989T1
Authority
AT
Austria
Prior art keywords
protein
purification process
protein purification
bind
proteins
Prior art date
Application number
AT02794380T
Other languages
English (en)
Inventor
Ganesh Vedantham
Clayton Brooks
Joanne Reeder
Andrew Goetze
Original Assignee
Immunex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27407561&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE425989(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Immunex Corp filed Critical Immunex Corp
Application granted granted Critical
Publication of ATE425989T1 publication Critical patent/ATE425989T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70575—NGF/TNF-superfamily, e.g. CD70, CD95L, CD153, CD154
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT02794380T 2001-12-21 2002-12-20 Proteinreinigungsverfahren ATE425989T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34336301P 2001-12-21 2001-12-21
US34718902P 2002-01-08 2002-01-08
US36427202P 2002-03-12 2002-03-12

Publications (1)

Publication Number Publication Date
ATE425989T1 true ATE425989T1 (de) 2009-04-15

Family

ID=27407561

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02794380T ATE425989T1 (de) 2001-12-21 2002-12-20 Proteinreinigungsverfahren

Country Status (11)

Country Link
US (2) US7122641B2 (de)
EP (1) EP1463942B2 (de)
JP (1) JP4363986B2 (de)
AT (1) ATE425989T1 (de)
AU (1) AU2002359816B2 (de)
CA (1) CA2469815C (de)
DE (1) DE60231651D1 (de)
ES (1) ES2322945T5 (de)
MX (1) MXPA04006053A (de)
PL (1) PL374311A1 (de)
WO (1) WO2003059935A2 (de)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406604B1 (en) 1999-11-08 2002-06-18 Norberto A. Guzman Multi-dimensional electrophoresis apparatus
US7329388B2 (en) 1999-11-08 2008-02-12 Princeton Biochemicals, Inc. Electrophoresis apparatus having staggered passage configuration
MXPA04006053A (es) * 2001-12-21 2005-03-31 Immunex Corp Metodos para purificacion de proteina.
EP2261230B1 (de) * 2002-09-11 2017-05-10 Chugai Seiyaku Kabushiki Kaisha Verfahren zur Proteinreinigung
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
EP1660094A4 (de) 2003-08-26 2009-09-16 Univ Colorado Hemmer der serinprotease-aktivität und ihre verwendung bei verfahren und zusammensetzungen zur behandlung von bakterieninfektionen
PT1678208E (pt) * 2003-10-27 2013-07-10 Wyeth Llc Remoção de agregados de elevado peso molecular utilizando cromatografia de hidroxiapatite
CA2563310A1 (en) 2003-11-07 2005-05-26 Princeton Biochemicals, Inc. Multi-dimensional electrophoresis apparatus
US8007725B2 (en) 2003-11-07 2011-08-30 Princeton Biochemicals, Inc. Electrophoresis apparatus having valve system
WO2005071410A1 (en) * 2004-01-13 2005-08-04 Tanox, Inc. Detecting and quantifying host cell proteins in recombinant protein products
JP4872327B2 (ja) * 2004-11-26 2012-02-08 東レ株式会社 改質されたインターロイキン−１１とその製造方法
WO2006099308A2 (en) * 2005-03-11 2006-09-21 Wyeth A method of weak partitioning chromatography
US20060240564A1 (en) * 2005-04-20 2006-10-26 Chia-Hui Shieh pH gradient ion exchange LC-MS and mass compatible buffers
ES2776657T3 (es) 2005-06-14 2020-07-31 Amgen Inc Formulaciones de proteínas autotamponantes
US7375188B2 (en) * 2005-07-29 2008-05-20 Mallinckrodt Baker, Inc. Vegetarian protein a preparation and methods thereof
MY185597A (en) * 2005-09-20 2021-05-24 Avantor Performance Mat Inc Vegetarian protein a preparation and methods thereof
EP1816201A1 (de) 2006-02-06 2007-08-08 CSL Behring GmbH Modifizierter Koagulationsfaktor VIIa mit verbesserter 'half-life'-Stabiltät
MX2008012896A (es) 2006-04-05 2008-10-14 Abbott Biotech Ltd Purificacion de anticuerpos.
WO2009017491A1 (en) * 2006-06-14 2009-02-05 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
US7939632B2 (en) 2006-06-14 2011-05-10 Csl Behring Gmbh Proteolytically cleavable fusion proteins with high molar specific activity
PL2032607T5 (pl) 2006-06-14 2017-10-31 Csl Behring Gmbh Rozszczepialne proteolitycznie białko fuzyjne obejmujące czynnik krzepnięcia krwi
EP1867660A1 (de) 2006-06-14 2007-12-19 CSL Behring GmbH Fusionsprotein welches proteolytisch geschnitten werden kann und einen Faktor der Blutgerinnungskaskade enthält
JP2010501622A (ja) * 2006-08-28 2010-01-21 アレス トレーディング ソシエテ アノニム Ｆｃ−融合タンパク質の精製法
FI2061803T4 (fi) 2006-08-28 2023-03-14 Process for the purification of fc-containing proteins
AU2007291282B2 (en) * 2006-08-28 2011-10-20 Ares Trading S.A. Process for the purification of Fc-fusion proteins
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
BRPI0716762A2 (pt) 2006-09-13 2013-09-24 Abbott Lab melhorias da cultura celular
US7488807B2 (en) * 2006-11-22 2009-02-10 3M Innovative Properties Company Antibody with protein A selectivity
US20100047252A1 (en) * 2006-11-22 2010-02-25 3M Innovative Properties Company Antibody with protein a selectivity
US8754194B2 (en) 2006-12-22 2014-06-17 Csl Behring Gmbh Modified coagulation factors with prolonged in vivo half-life
WO2009092014A1 (en) * 2008-01-18 2009-07-23 Gagnon Peter S Enhanced purification of antibodies and antibody fragments by apatite chromatography
US20100058173A1 (en) * 2008-08-28 2010-03-04 Kabushiki Kaisha Toshiba Display processing apparatus, display processing method, and computer program product
EP2334699B1 (de) * 2008-09-23 2013-09-11 F. Hoffmann-La Roche AG Aufreinigung von erythropoietin
TWI610936B (zh) * 2008-10-20 2018-01-11 艾伯維有限公司 使用蛋白質ａ親和性層析進行抗體之分離及純化
TW201028433A (en) 2008-10-20 2010-08-01 Abbott Lab Viral inactivation during purification of antibodies
US8058407B2 (en) * 2008-10-31 2011-11-15 Wyeth Llc Purification of acidic proteins using ceramic hydroxyapatite chromatography
US8945895B2 (en) 2009-07-31 2015-02-03 Baxter International Inc. Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
US8722615B2 (en) * 2009-12-02 2014-05-13 Acceleron Pharma, Inc. Compositions and methods for increasing serum half-life
WO2011088225A1 (en) 2010-01-15 2011-07-21 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
BR112012030530A2 (pt) * 2010-05-18 2022-12-20 Abbvie Inc Aparelho e processo para purificação de proteínas
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
EP2670704B1 (de) * 2011-02-02 2018-12-12 Bio-Rad Laboratories, Inc. Apatitoberflächenneutralisierung mit alkalischen lösungen
EP2702077A2 (de) 2011-04-27 2014-03-05 AbbVie Inc. Verfahren zur steuerung des galactosylierungsprofil von rekombinant exprimierten proteinen
US8911992B2 (en) * 2011-04-28 2014-12-16 Bio-Rad Laboratories, Inc. DNA removal in target molecule purification
US8883982B2 (en) 2011-06-08 2014-11-11 Acceleron Pharma, Inc. Compositions and methods for increasing serum half-life
CA2839917A1 (en) 2011-06-24 2012-12-27 The Regents Of The University Of Colorado, A Body Corporate Compositions, methods and uses for alpha-1 antitrypsin fusion molecules
BR112014000352A2 (pt) 2011-07-08 2017-02-14 Merck Sharp & Dohe Corp método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada
AU2013202648B2 (en) 2012-01-10 2016-05-19 Konkuk University Compositions, methods and uses for alpha-1 antitrypsin fusion molecules
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9505833B2 (en) 2012-04-20 2016-11-29 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
HK1211981A1 (en) 2012-09-02 2016-06-03 Abbvie Inc. Methods to control protein heterogeneity
EP2895610B1 (de) 2012-09-13 2019-11-06 Indiana University Research and Technology Corporation Zusammensetzungen und systeme zur induzierung von krankheitsresistenz bei pflanzen und verfahren zu ihrer verwendung
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
PT3395423T (pt) 2013-03-14 2023-11-14 Amgen Inc Remoção de ligandos escapados de cromatografia de afinidade
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
US9802822B2 (en) 2014-06-23 2017-10-31 Bio-Rad Laboratories, Inc. Apatite pretreatment
WO2015200299A2 (en) 2014-06-23 2015-12-30 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
GB2528301A (en) * 2014-07-17 2016-01-20 Univ Newcastle Biomolecule separation using apatite
US10814305B2 (en) * 2016-09-29 2020-10-27 Bio-Rad Laboratories, Inc. Agarose-filled ceramic apatite
EP3528787B1 (de) 2016-10-21 2026-01-07 Amgen Inc. Pharmazeutische formulierungen und verfahren zur herstellung davon
JP7065105B2 (ja) * 2017-02-09 2022-05-11 ブラッコ・スイス・ソシエテ・アノニム 可溶性ｐｓｇｌ－１タンパク質変異体の精製のための方法
US20220009959A1 (en) * 2018-11-26 2022-01-13 North Carolina State University Peptide ligands for capture of host cell proteins
WO2021163910A1 (en) 2020-02-19 2021-08-26 Bio-Rad Laboratories, Inc. Method of preparing polymer-filled chromatography resin
AU2021262609A1 (en) * 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein
MX2023006998A (es) 2020-12-22 2023-06-26 Amgen Inc Metodo de cultivo celular.
CN121712571A (zh) * 2023-08-15 2026-03-20 百时美施贵宝公司 陶瓷羟基磷灰石色谱流通法

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4745183A (en) * 1984-02-21 1988-05-17 Bio-Rad Laboratories, Inc. Use of hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
DE3738541A1 (de) * 1987-11-13 1989-05-24 Hoechst Ag Verfahren zur isolierung und reinigung von hirudin
JPH0774231B2 (ja) 1986-12-11 1995-08-09 東燃株式会社 抗体の分離方法
US4933286A (en) 1987-03-30 1990-06-12 Plant Cell Research Institute, Inc. Purification of apase-11 and retrieval of the nematode resistance gene
US5115101A (en) * 1988-06-08 1992-05-19 Miles Inc. Removal of protein A from antibody preparations
JPH022937A (ja) 1988-06-17 1990-01-08 Toray Ind Inc 酵素標識抗体の分離精製方法
JPH03139292A (ja) 1989-05-22 1991-06-13 Toray Ind Inc ヒトインタ―ロイキン―６に対するモノクロ―ナル抗体
US5395760A (en) * 1989-09-05 1995-03-07 Immunex Corporation DNA encoding tumor necrosis factor-α and -β receptors
DE10399023I2 (de) * 1989-09-12 2006-11-23 Ahp Mfg B V TFN-bindende Proteine
JPH03291300A (ja) 1989-12-28 1991-12-20 Mitsui Toatsu Chem Inc ポリクローナル抗体の分離方法
GB9007657D0 (en) * 1990-04-04 1990-05-30 Connaught Lab Purification of a pertussis outer membrane protein(omp69)
JPH04368399A (ja) 1991-06-12 1992-12-21 Toagosei Chem Ind Co Ltd 抗体の分離方法
JP2961986B2 (ja) 1991-08-22 1999-10-12 東亞合成株式会社 ポリクローナル抗体の分離精製方法
DE4205938A1 (de) * 1992-02-27 1993-09-02 Medac Klinische Spezialpraep Immunotoxine
JP3178080B2 (ja) 1992-04-28 2001-06-18 チッソ株式会社 抗体の分離精製方法
US5278284A (en) * 1992-05-14 1994-01-11 Miller Brewing Company Protein purification method
US6099830A (en) * 1994-08-09 2000-08-08 Zymogenetics, Inc. Methods for stimulating erythropoiesis using hematopoietic proteins
US5641655A (en) * 1994-11-30 1997-06-24 Zymogenetics, Inc. Methods for producing thrombopoietin polypeptides using a mammalian tissue plasminogen activator secretory peptide
JPH08188599A (ja) 1995-01-09 1996-07-23 Toagosei Co Ltd ＩｇＹ抗体の分離精製方法
US5744587A (en) * 1995-06-07 1998-04-28 Zymogenetics, Inc. Method for purifying thrombopoietin
DE69739673D1 (de) * 1996-11-27 2009-12-31 Genentech Inc Affinitätsreinigung von Polypeptid-Proteinen auf einer Protein A Matrix
PT998486E (pt) * 1997-06-13 2007-08-21 Genentech Inc ''recuperação de proteínas por cromatografia seguida de filtração numa camada carregada''
MXPA04006053A (es) * 2001-12-21 2005-03-31 Immunex Corp Metodos para purificacion de proteina.
WO2003059936A2 (en) * 2002-01-10 2003-07-24 Syngenta Participations Ag Phytophthora Infestans CDC 14 Phosphatase Gene, Protein and Methods of Use

Also Published As

Publication number Publication date
AU2002359816B2 (en) 2006-07-13
WO2003059935A2 (en) 2003-07-24
MXPA04006053A (es) 2005-03-31
EP1463942B2 (de) 2012-06-20
CA2469815C (en) 2011-05-03
DE60231651D1 (de) 2009-04-30
ES2322945T3 (es) 2009-07-02
WO2003059935A3 (en) 2003-10-16
CA2469815A1 (en) 2003-07-24
PL374311A1 (en) 2005-10-03
US20070010661A1 (en) 2007-01-11
US20030166869A1 (en) 2003-09-04
AU2002359816A1 (en) 2003-07-30
US7476722B2 (en) 2009-01-13
ES2322945T5 (es) 2012-10-17
EP1463942A4 (de) 2005-01-19
EP1463942A2 (de) 2004-10-06
EP1463942B1 (de) 2009-03-18
JP4363986B2 (ja) 2009-11-11
US7122641B2 (en) 2006-10-17
JP2005538686A (ja) 2005-12-22

Similar Documents

Publication Publication Date Title
ATE425989T1 (de) Proteinreinigungsverfahren
ATE363491T1 (de) Reinigung von antikörpern durch protein a und ionenaustauschromatographie
DK0746398T3 (da) Antistofrensning
KR950700779A (ko) 단백질의 정제방법(Protein purification)
ATE278785T1 (de) Neues verfahren zur gewinnung und reinigung von fusionsproteinen
PT1308455E (pt) Composicao compreendendo anticorpos anti-her2
DK0458841T3 (da) Fremgangsmåde til anvendelse og fremstilling af peptider
NO20025354L (no) Pattedyr-reseptorproteiner; beslektede reagenser og fremgangsmåter
DE59814468D1 (de) Verwendung monolithischer sorbentien für präparative chromatographische trennverfahren
WO2003104418A3 (en) Reconstituted polypeptides
ATE361930T1 (de) Proteinreinigung mittels chromatographie und darauffolgende filtration auf beladener schicht
ES8506902A1 (es) Un procedimiento para la purificacion de un antigeno.
ATE419361T1 (de) Menschliche monoklonale antikörper spezifisch für antigen e2 des hepatitis c virus
DK1115745T3 (da) Oprensning af fibrinogen fra væsker ved udfældning og hydrofob interaktionskromatografi
DE50011030D1 (de) Verfahren zur chromatographischen fraktionierung von plasma oder serum, so erhaltene präparate und deren verwendung
ATE250966T1 (de) Adsoptionschromatographie
DE59508137D1 (de) Spezifische bindungssubstanzen für antikörper und deren verwendung für immunoassays oder vakzine
MXPA05011577A (es) Purificacion de afinidad a ciclodextrina.
WO1991015582A3 (en) Purified rap gaps, rap gap sequences, and uses thereof
ATE193021T1 (de) Lipoprotein (a)-peptide und deren verwendung
BRPI0504527A (pt) processo de purificação de proteìnas antigênicas isoladas de taenia crassiceps, kit para o diagnóstico de cisticercose e método de diagnóstico da cisticercose em animais mamìferos

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties