ES8506902A1 - Un procedimiento para la purificacion de un antigeno. - Google Patents

Un procedimiento para la purificacion de un antigeno.

Info

Publication number
ES8506902A1
ES8506902A1 ES528000A ES528000A ES8506902A1 ES 8506902 A1 ES8506902 A1 ES 8506902A1 ES 528000 A ES528000 A ES 528000A ES 528000 A ES528000 A ES 528000A ES 8506902 A1 ES8506902 A1 ES 8506902A1
Authority
ES
Spain
Prior art keywords
antigen
antibody
environment
monoclonal antibodies
affinity purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES528000A
Other languages
English (en)
Other versions
ES528000A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hybritech Inc
Original Assignee
Hybritech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybritech Inc filed Critical Hybritech Inc
Publication of ES8506902A1 publication Critical patent/ES8506902A1/es
Publication of ES528000A0 publication Critical patent/ES528000A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PROCEDIMIENTO PARA LA PURIFICACION DE UN ANTIGENO.COMPRENDE: A) SELECCIONAR UN ANTICUERPO QUE TIENE UNA GRAN AFINIDAD POR EL ANTIGENO EN UN PRIMER MEDIO AMBIENTE Y UNA BAJA AFINIDAD POR EL ANTIGENO EN UN SEGUNDO MEDIO AMBIENTE, SIN QUE NINGUNO DE LOS MEDIOS AMBIENTES ALTERE DE MODO SUSTANCIALMENTE IRREVERSIBLE LAS PROPIEDADES INMUNOQUIMICAS O BIOLOGICAS DEL ANTIGENO O DEL ANTICUERPO; B) INMOVILIZAR EL ANTICUERPO SOBRE UN SOPORTE SOLIDO; C) FIJAR EL ANTIGENO AL ANTICUERPO EN EL PRIMER MEDIO AMBIENTE, CONSISTENTE EN UN LIQUIDO A UN PH DETERMINADO; D) SEPARAR LAS IMPUREZAS NO FIJADAS DEL ANTIGENO FIJADO, Y E) ELUIR EL ANTIGENO EN UNA FORMA PURIFICADA DESDE EL ANTICUERPO INMOVILIZADO, UTILIZANDO COMO ELUYENTE EL SEGUNDO MEDIO AMBIENTE, CONSISTENTE EN UNA SOLUCION QUE CONTIENE AGENTES CAOTROPICOS SELECCIONADOS ENTRE BRK, KI Y GUANIDINA Y UN PH ENTRE 4 Y 10,5.
ES528000A 1982-04-12 1983-12-13 Un procedimiento para la purificacion de un antigeno. Granted ES528000A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36778182A 1982-04-12 1982-04-12

Publications (2)

Publication Number Publication Date
ES8506902A1 true ES8506902A1 (es) 1985-08-01
ES528000A0 ES528000A0 (es) 1985-08-01

Family

ID=23448565

Family Applications (3)

Application Number Title Priority Date Filing Date
ES521371A Expired ES8404858A1 (es) 1982-04-12 1983-04-11 Un procedimiento para la purificacion de un anticuerpo.
ES528000A Granted ES528000A0 (es) 1982-04-12 1983-12-13 Un procedimiento para la purificacion de un antigeno.
ES528001A Expired ES8706964A1 (es) 1982-04-12 1983-12-13 Un procedimiento para fraccionar anticuerpos de suero.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES521371A Expired ES8404858A1 (es) 1982-04-12 1983-04-11 Un procedimiento para la purificacion de un anticuerpo.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES528001A Expired ES8706964A1 (es) 1982-04-12 1983-12-13 Un procedimiento para fraccionar anticuerpos de suero.

Country Status (11)

Country Link
EP (1) EP0105359A4 (es)
AT (1) AT393386B (es)
AU (1) AU622099B2 (es)
CA (1) CA1209907A (es)
CH (1) CH672028A5 (es)
DK (1) DK192191A (es)
ES (3) ES8404858A1 (es)
FI (1) FI88403C (es)
GB (1) GB2128630B (es)
IT (1) IT1219779B (es)
WO (1) WO1983003678A1 (es)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8328918D0 (en) * 1983-10-28 1983-11-30 Unilever Plc Alkaline phosphatase
GB8331071D0 (en) * 1983-11-22 1983-12-29 Karayiannis P Assay for dna/rna
US4666865A (en) * 1984-01-13 1987-05-19 Centocor, Inc. Immunoassay for biologically active human interferon-gamma employing unique monoclonal antibodies
US4789631A (en) * 1984-02-17 1988-12-06 Synbiotics Corporation Immunoassay for anti-dirofilaria immitis antibody
GB8426468D0 (en) * 1984-10-19 1984-11-28 Technology Licence Co Ltd Monoclonal antibodies
AU5259786A (en) * 1985-01-30 1986-08-07 United States Of America, The Monoclonal antibodies against chlamydial genes specific lipopolysaccharide
US4818687A (en) * 1985-02-28 1989-04-04 Massachusetts Institute Of Technology Affinity column and process for detection of low molecular weight toxic substances
US4859611A (en) * 1985-02-28 1989-08-22 Massachusetts Institute Of Technology Affinity column and process for detection of low molecular weight toxic substances
IL75828A (en) * 1985-07-17 1991-06-10 Univ Ramot Immobilization by biologically active proteins
NZ218336A (en) * 1985-12-09 1991-08-27 Kirin Amgen Inc Monoclonal antibodies to human pluripotent granulocyte colony stimulating factor (hpg-csf)
US5175255A (en) * 1987-03-23 1992-12-29 Amgen Inc. Methods for purification of platelet-derived growth factor
US5190859A (en) * 1987-02-26 1993-03-02 Dana-Farber Cancer Institute, Inc. Purification of LFA-3
SE468653B (sv) * 1987-03-06 1993-02-22 Perstorp Biolytica Adsorbent foer isokratisk affinitetskromatografi
JPS6463599A (en) * 1987-05-01 1989-03-09 Xoma Corp Purification of immunoglobulin by selective anti-idiotype bond
ATE76974T1 (de) * 1987-07-14 1992-06-15 Univ Manchester Verfahren zur diagnose von infektionen, bei welchem lipopolyscaccharidantigene bestimmt werden.
CA1323567C (en) * 1987-10-05 1993-10-26 Gene R. Nathans Method for purification of antibodies
US5582862A (en) * 1988-04-04 1996-12-10 General Hospital Corporation Antibodies that bind to α2-antiplasmin crosslinked to fibrin which do not inhibit plasma α2-antiplasmin
US5372812A (en) * 1988-04-04 1994-12-13 The General Hospital Corporation Composition and method for acceleration of clot lysis
JP2001515345A (ja) * 1996-09-20 2001-09-18 ザ・ジェネラル・ホスピタル・コーポレイション アルファ―２―抗プラスミンに対する抗体を用いてフィブリン溶解を増進するための組成物と方法
TWI564021B (zh) * 2008-04-11 2017-01-01 Chugai Pharmaceutical Co Ltd Repeated binding of antigen to antigen binding molecules
MX365235B (es) 2010-11-30 2019-05-28 Chugai Pharmaceutical Co Ltd Molécula de unión a antígeno capaz de unir repetidamente a la pluralidad de moléculas de antígeno.
US20140093496A1 (en) 2011-02-25 2014-04-03 Chugai Seiyaku Kabushiki Kaisha Fc-gamma-RIIb-SPECIFIC Fc ANTIBODY
US20150050269A1 (en) 2011-09-30 2015-02-19 Chugai Seiyaku Kabushiki Kaisha Antigen-binding molecule promoting disappearance of antigens having plurality of biological activities
US20150056182A1 (en) 2011-11-30 2015-02-26 Chugai Seiyaku Kabushiki Kaisha Drug containing carrier into cell for forming immune complex
CN113045650A (zh) 2014-12-19 2021-06-29 中外制药株式会社 抗-c5抗体及使用方法
CA2963760A1 (en) 2014-12-19 2016-06-23 Yoshinao Ruike Anti-myostatin antibodies, polypeptides containing variant fc regions, and methods of use
EP3394098A4 (en) 2015-12-25 2019-11-13 Chugai Seiyaku Kabushiki Kaisha ANTI-MYOSTATIN ANTIBODIES AND METHODS OF USE
CA3026050A1 (en) 2016-08-05 2018-02-08 Chugai Seiyaku Kabushiki Kaisha Composition for prophylaxis or treatment of il-8 related diseases
JP7191833B2 (ja) 2017-01-30 2022-12-19 中外製薬株式会社 抗スクレロスチン抗体およびその使用
CN110724204B (zh) * 2019-11-18 2021-10-22 广东菲鹏制药股份有限公司 Fc融合蛋白的纯化方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4172124A (en) * 1978-04-28 1979-10-23 The Wistar Institute Method of producing tumor antibodies
ATE14269T1 (de) * 1981-02-26 1985-08-15 Unilever Nv Verfahren und vorrichtung zur rueckgewinnung von immunoglobulinen.
US4474893A (en) * 1981-07-01 1984-10-02 The University of Texas System Cancer Center Recombinant monoclonal antibodies
US4361509A (en) * 1981-12-14 1982-11-30 Scripps Clinic And Research Foundation Ultrapurification of factor VIII using monoclonal antibodies

Also Published As

Publication number Publication date
FI88403C (fi) 1993-05-10
CH672028A5 (es) 1989-10-13
AU7256987A (en) 1987-09-03
AT393386B (de) 1991-10-10
IT1219779B (it) 1990-05-24
FI834530A (fi) 1983-12-09
GB8332644D0 (en) 1984-01-11
IT8320550A0 (it) 1983-04-12
ES521371A0 (es) 1984-05-16
ES528001A0 (es) 1987-07-01
ES8404858A1 (es) 1984-05-16
WO1983003678A1 (en) 1983-10-27
AU622099B2 (en) 1992-04-02
ATA901983A (de) 1991-03-15
FI834530A0 (fi) 1983-12-09
GB2128630B (en) 1986-08-28
ES8706964A1 (es) 1987-07-01
EP0105359A1 (en) 1984-04-18
CA1209907A (en) 1986-08-19
FI88403B (fi) 1993-01-29
DK192191D0 (da) 1991-11-26
GB2128630A (en) 1984-05-02
ES528000A0 (es) 1985-08-01
EP0105359A4 (en) 1984-08-20
DK192191A (da) 1991-11-26

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970505