WO1991015582A3 - Purified rap gaps, rap gap sequences, and uses thereof - Google Patents

Purified rap gaps, rap gap sequences, and uses thereof Download PDF

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Publication number
WO1991015582A3
WO1991015582A3 PCT/US1991/001763 US9101763W WO9115582A3 WO 1991015582 A3 WO1991015582 A3 WO 1991015582A3 US 9101763 W US9101763 W US 9101763W WO 9115582 A3 WO9115582 A3 WO 9115582A3
Authority
WO
WIPO (PCT)
Prior art keywords
rap
gaps
methods
proteins
purified
Prior art date
Application number
PCT/US1991/001763
Other languages
French (fr)
Other versions
WO1991015582A2 (en
Inventor
Paul G Polakis
Francis P Mccormick
Bonnee Rubinfield
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Publication of WO1991015582A2 publication Critical patent/WO1991015582A2/en
Publication of WO1991015582A3 publication Critical patent/WO1991015582A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C07K14/4706—Guanosine triphosphatase activating protein, GAP

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This invention presents substantially purified rap GAPs (ras proximate guanine triphosphatase activating proteins); and methods for purifying the proteins using either successive chromatographic steps or monoclonal antibodies to the proteins or to the partial amino acid sequences of the protein. The DNA and predicted amino acid sequences of a rap GAP are presented, as well as antibodies against rap GAP and methods for producing such antibodies. Also presented are methods for identifying anti-cancer therapeutics using these rap GAPs.
PCT/US1991/001763 1990-03-30 1991-03-15 PURIFIED RAP GAPs, RAP GAP SEQUENCES, AND USES THEREOF WO1991015582A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US50233290A 1990-03-30 1990-03-30
US502,332 1990-03-30
US64280191A 1991-01-18 1991-01-18
US642,801 1991-01-18

Publications (2)

Publication Number Publication Date
WO1991015582A2 WO1991015582A2 (en) 1991-10-17
WO1991015582A3 true WO1991015582A3 (en) 1991-12-12

Family

ID=27054118

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/001763 WO1991015582A2 (en) 1990-03-30 1991-03-15 PURIFIED RAP GAPs, RAP GAP SEQUENCES, AND USES THEREOF

Country Status (3)

Country Link
EP (1) EP0537155A1 (en)
AU (1) AU7554691A (en)
WO (1) WO1991015582A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830684A (en) * 1988-08-10 1998-11-03 Chiron Corporation Native type II GAP, methods for purifying various GAPs and uses of GAPs to diagnose cancer
CA2065634C (en) * 1991-04-11 1997-06-03 Alec A. Piplani Endovascular graft having bifurcation and apparatus and method for deploying the same
US6072154A (en) 1996-09-05 2000-06-06 Medtronic, Inc. Selectively activated shape memory device
US6238881B1 (en) 1996-11-06 2001-05-29 Onyx Pharmaceuticals, Inc. Nucleic acids and polypeptides related to a guanine exchange factor of Rho GTPase
AU1744900A (en) * 1998-11-23 2000-06-13 Incyte Pharmaceuticals, Inc. Gtpase associated proteins
WO2000075320A2 (en) * 1999-06-07 2000-12-14 Lexicon Genetics Incorporated Human genes and proteins encoded thereby

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990000607A2 (en) * 1988-07-08 1990-01-25 Cetus Corporation Gap gene sequences and diagnostic uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990000607A2 (en) * 1988-07-08 1990-01-25 Cetus Corporation Gap gene sequences and diagnostic uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Nature, vol. 335, 1 September 1988, U.S. Vogel et al.: "Cloning of bovine GAP and its interaction with oncogenic ras p21", pages 90-93, see the whole article *
Proc. Natl. Acad. Sci. USA, vol. 85, July 1988, Biochemistry, J.B. Gibbs et al.: "Purification of ras GTPase activating protein from bovine brain", pages 5026-30, see the whole document *
Proc. Natl. Acad. Sci. USA, vol. 88, January 1991, Biochemistry, P.G. Polakis et al.: "Purification of a plasma membrane-associated GTPase-activating protein specific for rap1/Krev-1 from HL60 cells", pages 239-243, see the whole document *
Science, vol. 242, 23 December 1988, M. Trahey et al.: "Molecular cloning of two types of GAP complementary DNA from human placenta", pages 1697-1700, see the whole article *
The Journal of Biological Chemistry, vol. 264, no. 16, 5 June 1989, The American Society for Biochemistry and Molecular Biology, Inc. (US), A. Kikuchi et al.: "Purification and characterization from bovine brain cytosol of two GTPase-activating proteins specific for smg p21, a GTP-binding protein having the same effector domain as c-ras p21s", pages 9133-9136, see the whole document (cited in the application) *

Also Published As

Publication number Publication date
EP0537155A1 (en) 1993-04-21
AU7554691A (en) 1991-10-30
WO1991015582A2 (en) 1991-10-17

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