DE60231651D1 - Proteinreinigungsverfahren - Google Patents

Proteinreinigungsverfahren

Info

Publication number
DE60231651D1
DE60231651D1 DE60231651T DE60231651T DE60231651D1 DE 60231651 D1 DE60231651 D1 DE 60231651D1 DE 60231651 T DE60231651 T DE 60231651T DE 60231651 T DE60231651 T DE 60231651T DE 60231651 D1 DE60231651 D1 DE 60231651D1
Authority
DE
Germany
Prior art keywords
protein
cleaning process
bind
proteins
hydroxyapatite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60231651T
Other languages
English (en)
Inventor
Ganesh Vedantham
Clayton A Brooks
Joanne M Reeder
Andrew M Goetze
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immunex Corp
Original Assignee
Immunex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27407561&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60231651(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Immunex Corp filed Critical Immunex Corp
Application granted granted Critical
Publication of DE60231651D1 publication Critical patent/DE60231651D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70575—NGF/TNF-superfamily, e.g. CD70, CD95L, CD153, CD154
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE60231651T 2001-12-21 2002-12-20 Proteinreinigungsverfahren Expired - Lifetime DE60231651D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US34336301P 2001-12-21 2001-12-21
US34718902P 2002-01-08 2002-01-08
US36427202P 2002-03-12 2002-03-12
PCT/US2002/041159 WO2003059935A2 (en) 2001-12-21 2002-12-20 Methods for purifying protein

Publications (1)

Publication Number Publication Date
DE60231651D1 true DE60231651D1 (de) 2009-04-30

Family

ID=27407561

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60231651T Expired - Lifetime DE60231651D1 (de) 2001-12-21 2002-12-20 Proteinreinigungsverfahren

Country Status (11)

Country Link
US (2) US7122641B2 (de)
EP (1) EP1463942B2 (de)
JP (1) JP4363986B2 (de)
AT (1) ATE425989T1 (de)
AU (1) AU2002359816B2 (de)
CA (1) CA2469815C (de)
DE (1) DE60231651D1 (de)
ES (1) ES2322945T5 (de)
MX (1) MXPA04006053A (de)
PL (1) PL374311A1 (de)
WO (1) WO2003059935A2 (de)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406604B1 (en) 1999-11-08 2002-06-18 Norberto A. Guzman Multi-dimensional electrophoresis apparatus
US7329388B2 (en) 1999-11-08 2008-02-12 Princeton Biochemicals, Inc. Electrophoresis apparatus having staggered passage configuration
MXPA04006053A (es) * 2001-12-21 2005-03-31 Immunex Corp Metodos para purificacion de proteina.
JP5525118B2 (ja) * 2002-09-11 2014-06-18 中外製薬株式会社 タンパク質精製方法
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
EP3192872A1 (de) 2003-08-26 2017-07-19 The Regents of the University of Colorado, a body corporate Inhibitoren der serinproteaseaktivität und deren verwendung in verfahren und zusammensetzungen zur behandlung bakterieller infektionen
PT1678208E (pt) * 2003-10-27 2013-07-10 Wyeth Llc Remoção de agregados de elevado peso molecular utilizando cromatografia de hidroxiapatite
US8007724B2 (en) 2003-11-07 2011-08-30 Princeton Biochemicals, Inc. Electrophoresis apparatus having at least one auxiliary buffer passage
EP1706735B1 (de) 2003-11-07 2017-01-04 Princeton Biochemicals, Inc. Mehrdimensionale elektrophoresevorrichtung
WO2005071410A1 (en) * 2004-01-13 2005-08-04 Tanox, Inc. Detecting and quantifying host cell proteins in recombinant protein products
JP4872327B2 (ja) * 2004-11-26 2012-02-08 東レ株式会社 改質されたインターロイキン−１１とその製造方法
DK1869065T3 (da) 2005-03-11 2020-06-08 Wyeth Llc Fremgangsmåde til kromatografi med svag opdeling
US20060240564A1 (en) * 2005-04-20 2006-10-26 Chia-Hui Shieh pH gradient ion exchange LC-MS and mass compatible buffers
KR20080031684A (ko) 2005-06-14 2008-04-10 암젠 인코포레이티드 자가 - 완충성 단백질 제형
US7375188B2 (en) 2005-07-29 2008-05-20 Mallinckrodt Baker, Inc. Vegetarian protein a preparation and methods thereof
MY185597A (en) * 2005-09-20 2021-05-24 Avantor Performance Mat Inc Vegetarian protein a preparation and methods thereof
EP1816201A1 (de) 2006-02-06 2007-08-08 CSL Behring GmbH Modifizierter Koagulationsfaktor VIIa mit verbesserter 'half-life'-Stabiltät
WO2007117490A2 (en) 2006-04-05 2007-10-18 Abbott Biotechnology Ltd. Antibody purification
EP1867660A1 (de) 2006-06-14 2007-12-19 CSL Behring GmbH Fusionsprotein welches proteolytisch geschnitten werden kann und einen Faktor der Blutgerinnungskaskade enthält
EP3896090B1 (de) 2006-06-14 2022-01-12 CSL Behring GmbH Proteolytisch spaltbare fusionsproteine die einen blutgerinnungsfaktor enthalten
JP2010510963A (ja) * 2006-06-14 2010-04-08 グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー セラミックヒドロキシアパタイトを使用する抗体の精製方法
US7939632B2 (en) 2006-06-14 2011-05-10 Csl Behring Gmbh Proteolytically cleavable fusion proteins with high molar specific activity
US8513393B2 (en) 2006-08-28 2013-08-20 Ares Trading S.A. Process for the purification of Fc-containing proteins
CN101541825B (zh) * 2006-08-28 2013-08-14 阿雷斯贸易股份有限公司 Fc融合蛋白的纯化方法
JP2010501622A (ja) * 2006-08-28 2010-01-21 アレス トレーディング ソシエテ アノニム Ｆｃ−融合タンパク質の精製法
SG10201510384UA (en) 2006-09-13 2016-01-28 Abbvie Inc Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
US7488807B2 (en) * 2006-11-22 2009-02-10 3M Innovative Properties Company Antibody with protein A selectivity
US20100047252A1 (en) * 2006-11-22 2010-02-25 3M Innovative Properties Company Antibody with protein a selectivity
KR101542752B1 (ko) 2006-12-22 2015-08-10 체에스엘 베링 게엠베하 연장된 생체내 반감기를 갖는 변형된 응고 인자
EP2238154B1 (de) * 2008-01-18 2015-09-16 Bio-Rad Laboratories, Inc. Erweiterte reinigung phosphorylierter und nicht phosphorylierter biomoleküle durch apatit-chromatographie
US20100058173A1 (en) * 2008-08-28 2010-03-04 Kabushiki Kaisha Toshiba Display processing apparatus, display processing method, and computer program product
ES2435272T3 (es) * 2008-09-23 2013-12-17 F. Hoffmann-La Roche Ag Purificación de la eritropoyetina
CA2738499A1 (en) 2008-10-20 2010-04-29 Abbott Laboratories Viral inactivation during purification of antibodies
AU2009347206C1 (en) 2008-10-20 2016-12-08 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
US8058407B2 (en) * 2008-10-31 2011-11-15 Wyeth Llc Purification of acidic proteins using ceramic hydroxyapatite chromatography
US8945895B2 (en) 2009-07-31 2015-02-03 Baxter International Inc. Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
BR112012013330A2 (pt) * 2009-12-02 2017-03-28 Acceleron Pharma Inc composições e métodos para aumentar meia vida do soro de proteínas de fusão fc
US8951807B2 (en) 2010-01-15 2015-02-10 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
SG10201503899SA (en) * 2010-05-18 2015-06-29 Abbvie Inc Apparatus And Process Of Purification Of Proteins
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
EP2670704B1 (de) * 2011-02-02 2018-12-12 Bio-Rad Laboratories, Inc. Apatitoberflächenneutralisierung mit alkalischen lösungen
EP2702077A2 (de) 2011-04-27 2014-03-05 AbbVie Inc. Verfahren zur steuerung des galactosylierungsprofil von rekombinant exprimierten proteinen
US8911992B2 (en) * 2011-04-28 2014-12-16 Bio-Rad Laboratories, Inc. DNA removal in target molecule purification
EP2718328A4 (de) 2011-06-08 2014-12-24 Acceleron Pharma Inc Zusammensetzungen und verfahren zur erhöhung der halbwertszeit von serum
US9938353B2 (en) 2011-06-24 2018-04-10 The Regents Of The University Of Colorado, A Body Corporate Compositions, methods and uses for alpha-1 antitrypsin fusion molecules
US9556258B2 (en) 2011-07-08 2017-01-31 Merck Sharp & Dohme Corp. Purification of fusion proteins
CA2896951A1 (en) 2012-01-10 2013-07-18 The Regents Of The University Of Colorado, A Body Corporate Compositions, methods and uses for alpha-1 antitrypsin fusion molecules
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
US9206390B2 (en) 2012-09-02 2015-12-08 Abbvie, Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
WO2014042923A1 (en) 2012-09-13 2014-03-20 Indiana University Research & Technology Corporation Compositions and systems for conferring disease resistance in plants and methods of use thereof
CA2905010A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
SI2969099T2 (sl) * 2013-03-14 2022-04-29 Amgen Inc. Odstranjevanje izluženega afinitetnega purifikacijskega liganda
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
EP3052640A2 (de) 2013-10-04 2016-08-10 AbbVie Inc. Verwendung von metallionen zur modulation von proteinglykosylierungsprofilen rekombinanter proteine
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
CN105392569B (zh) 2014-06-23 2019-08-20 生物辐射实验室股份有限公司 磷灰石预处理
WO2015200278A1 (en) 2014-06-23 2015-12-30 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
GB2528301A (en) * 2014-07-17 2016-01-20 Univ Newcastle Biomolecule separation using apatite
CN109803736B (zh) * 2016-09-29 2022-02-08 生物辐射实验室股份有限公司 琼脂糖填充的陶瓷磷灰石
BR112019007858A2 (pt) 2016-10-21 2019-07-02 Amgen Inc formulações farmacêuticas e métodos para produzir as mesmas
EP3887386A4 (de) * 2018-11-26 2022-11-30 North Carolina State University Peptidliganden zur einfangen von wirtszellproteinen
JP2023523823A (ja) * 2020-05-01 2023-06-07 カシーヴ バイオサイエンシズ，リミテッド・ライアビリティ・カンパニー タンパク質の精製の改善されたプロセス
KR20230124578A (ko) 2020-12-22 2023-08-25 암젠 인크 세포 배양 방법

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4745183A (en) * 1984-02-21 1988-05-17 Bio-Rad Laboratories, Inc. Use of hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
DE3738541A1 (de) * 1987-11-13 1989-05-24 Hoechst Ag Verfahren zur isolierung und reinigung von hirudin
JPH0774231B2 (ja) 1986-12-11 1995-08-09 東燃株式会社 抗体の分離方法
US4933286A (en) 1987-03-30 1990-06-12 Plant Cell Research Institute, Inc. Purification of apase-11 and retrieval of the nematode resistance gene
US5115101A (en) * 1988-06-08 1992-05-19 Miles Inc. Removal of protein A from antibody preparations
JPH022937A (ja) 1988-06-17 1990-01-08 Toray Ind Inc 酵素標識抗体の分離精製方法
JPH03139292A (ja) 1989-05-22 1991-06-13 Toray Ind Inc ヒトインタ―ロイキン―６に対するモノクロ―ナル抗体
US5395760A (en) * 1989-09-05 1995-03-07 Immunex Corporation DNA encoding tumor necrosis factor-α and -β receptors
EP0939121B2 (de) * 1989-09-12 2007-12-26 AHP Manufacturing B.V. TFN-bindende Proteine
JPH03291300A (ja) 1989-12-28 1991-12-20 Mitsui Toatsu Chem Inc ポリクローナル抗体の分離方法
GB9007657D0 (en) * 1990-04-04 1990-05-30 Connaught Lab Purification of a pertussis outer membrane protein(omp69)
JPH04368399A (ja) 1991-06-12 1992-12-21 Toagosei Chem Ind Co Ltd 抗体の分離方法
JP2961986B2 (ja) 1991-08-22 1999-10-12 東亞合成株式会社 ポリクローナル抗体の分離精製方法
DE4205938A1 (de) * 1992-02-27 1993-09-02 Medac Klinische Spezialpraep Immunotoxine
JP3178080B2 (ja) 1992-04-28 2001-06-18 チッソ株式会社 抗体の分離精製方法
US5278284A (en) * 1992-05-14 1994-01-11 Miller Brewing Company Protein purification method
US6099830A (en) * 1994-08-09 2000-08-08 Zymogenetics, Inc. Methods for stimulating erythropoiesis using hematopoietic proteins
US5641655A (en) * 1994-11-30 1997-06-24 Zymogenetics, Inc. Methods for producing thrombopoietin polypeptides using a mammalian tissue plasminogen activator secretory peptide
JPH08188599A (ja) 1995-01-09 1996-07-23 Toagosei Co Ltd ＩｇＹ抗体の分離精製方法
US5744587A (en) * 1995-06-07 1998-04-28 Zymogenetics, Inc. Method for purifying thrombopoietin
IL130061A (en) * 1996-11-27 2001-09-13 Genentech Inc Affinity purification of polypeptide on protein a matrix
JP2001510483A (ja) * 1997-06-13 2001-07-31 ジェネンテク，インコーポレイテッド タンパク質の回収
MXPA04006053A (es) * 2001-12-21 2005-03-31 Immunex Corp Metodos para purificacion de proteina.
AU2003207496A1 (en) * 2002-01-10 2003-07-30 Syngenta Participations Ag Phytophthora infestans cdc 14 phosphatase gene, protein and methods of use

Also Published As

Publication number Publication date
ES2322945T5 (es) 2012-10-17
AU2002359816A1 (en) 2003-07-30
EP1463942A2 (de) 2004-10-06
WO2003059935A3 (en) 2003-10-16
US7122641B2 (en) 2006-10-17
US20070010661A1 (en) 2007-01-11
JP4363986B2 (ja) 2009-11-11
EP1463942B1 (de) 2009-03-18
MXPA04006053A (es) 2005-03-31
US20030166869A1 (en) 2003-09-04
JP2005538686A (ja) 2005-12-22
US7476722B2 (en) 2009-01-13
EP1463942A4 (de) 2005-01-19
AU2002359816B2 (en) 2006-07-13
CA2469815A1 (en) 2003-07-24
CA2469815C (en) 2011-05-03
WO2003059935A2 (en) 2003-07-24
ES2322945T3 (es) 2009-07-02
PL374311A1 (en) 2005-10-03
EP1463942B2 (de) 2012-06-20
ATE425989T1 (de) 2009-04-15

Similar Documents

Publication Publication Date Title
DE60231651D1 (de) Proteinreinigungsverfahren
ES2177632T3 (es) Purificacion de anticuerpos.
KR950700779A (ko) 단백질의 정제방법(Protein purification)
ATE363491T1 (de) Reinigung von antikörpern durch protein a und ionenaustauschromatographie
CY1119024T1 (el) Καθαρισμος πρωτεϊνης
PT1308455E (pt) Composicao compreendendo anticorpos anti-her2
DK0458841T3 (da) Fremgangsmåde til anvendelse og fremstilling af peptider
IS2508B (is) Mótefni gegn ED-B hneppinu í fíbrónektíni, smíði þeirra og notkanir
NO20025354L (no) Pattedyr-reseptorproteiner; beslektede reagenser og fremgangsmåter
HUP0202532A2 (hu) Ko-stimulátor szignál-transzdukciós molekula (AILIM) elleni humán monoklonális antitest és gyógyászati alkalmazása
WO2002083710A3 (en) Removal of bacterial endotoxin in a protein solution by immobilized metal affinity chromatography
DE69633563D1 (de) Neues verfahren zur gewinnung und reinigung von fusionsproteinen
ATE361930T1 (de) Proteinreinigung mittels chromatographie und darauffolgende filtration auf beladener schicht
DE69739187D1 (de) Menschliche monoklonale antikörper spezifisch für antigen e2 des hepatitis c virus
DK1115745T3 (da) Oprensning af fibrinogen fra væsker ved udfældning og hydrofob interaktionskromatografi
ATE250966T1 (de) Adsoptionschromatographie
DE59508137D1 (de) Spezifische bindungssubstanzen für antikörper und deren verwendung für immunoassays oder vakzine
ATE302793T1 (de) Verfahren zur chromatographischen fraktionierung von plasma oder serum, so erhaltene präparate und deren verwendung
ATE246204T1 (de) Verfahren zur trennung und/oder isolierung von plasmaproteinen mit annularer chromatographie
DK1171157T3 (da) Anvendelse af glucose-6-phosphat-isomerase og antistoffer derimod til diagnosticering og behandling af arthritis, og test for anti-arthritis-forbindelser
ES2135480T3 (es) Nueva molecula de celula endotelial que media la union de linfocitos en el hombre.
ATE193021T1 (de) Lipoprotein (a)-peptide und deren verwendung

Legal Events

Date Code Title Description
8363 Opposition against the patent