DE60300356D1 - Ein Verfahren zur Trennung und Reinigung von RNA - Google Patents
Ein Verfahren zur Trennung und Reinigung von RNAInfo
- Publication number
- DE60300356D1 DE60300356D1 DE60300356T DE60300356T DE60300356D1 DE 60300356 D1 DE60300356 D1 DE 60300356D1 DE 60300356 T DE60300356 T DE 60300356T DE 60300356 T DE60300356 T DE 60300356T DE 60300356 D1 DE60300356 D1 DE 60300356D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- separating
- purifying
- purifying rna
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- 239000000203 mixture Substances 0.000 abstract 2
- 239000007790 solid phase Substances 0.000 abstract 2
- 229920002521 macromolecule Polymers 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002210833 | 2002-07-19 | ||
| JP2002210833A JP3983125B2 (ja) | 2002-07-19 | 2002-07-19 | 核酸の分離精製方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60300356D1 true DE60300356D1 (de) | 2005-04-07 |
| DE60300356T2 DE60300356T2 (de) | 2006-04-06 |
Family
ID=29774669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60300356T Expired - Lifetime DE60300356T2 (de) | 2002-07-19 | 2003-07-18 | Ein Verfahren zur Trennung und Reinigung von RNA |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7252939B2 (de) |
| EP (1) | EP1382677B1 (de) |
| JP (1) | JP3983125B2 (de) |
| AT (1) | ATE290076T1 (de) |
| DE (1) | DE60300356T2 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006238854A (ja) * | 2004-03-26 | 2006-09-14 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| EP1727900A4 (de) * | 2004-03-26 | 2008-05-28 | Fujifilm Corp | Verfahren zur selektiven trennung und reinigungvon rna sowie verfahren zur trennung und reinigung von nukleinsäure |
| JP2006217839A (ja) * | 2005-02-09 | 2006-08-24 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| ATE541037T1 (de) | 2004-08-25 | 2012-01-15 | Fujifilm Corp | Verfahren zur trennung und reinigung von nukleinsäure |
| JP2006087429A (ja) * | 2004-08-25 | 2006-04-06 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| JP4810164B2 (ja) * | 2004-09-03 | 2011-11-09 | 富士フイルム株式会社 | 核酸分離精製方法 |
| CN101098962A (zh) * | 2005-01-31 | 2008-01-02 | 富士胶片株式会社 | 样品溶液的制备方法和样品溶液的制备装置 |
| JP4825527B2 (ja) * | 2006-02-03 | 2011-11-30 | 富士フイルム株式会社 | 核酸の分離精製方法 |
| JP4825528B2 (ja) * | 2006-02-03 | 2011-11-30 | 富士フイルム株式会社 | 核酸の分離精製方法 |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
| KR101702154B1 (ko) | 2009-03-24 | 2017-02-03 | 유니버시티 오브 시카고 | 반응을 수행하기 위한 장치 |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5804684A (en) * | 1995-08-24 | 1998-09-08 | The Theobald Smith Research Institute, Inc. | Method for isolating nucleic acids |
| US20020104801A1 (en) | 1998-04-06 | 2002-08-08 | Nicolas Voute | Small dense microporous solid support materials, their preparation,and use for purification of large macromolecules and bioparticles |
| WO2001062976A1 (en) | 2000-02-23 | 2001-08-30 | Wen Shao | Rapid nucleic acid separation, isolation and purification methods |
| ATE422542T1 (de) | 2001-02-16 | 2009-02-15 | Cortex Biochem Inc | Magnetische isolierung und reinigung von nukleinsäuren |
| AU2002211719B2 (en) | 2001-10-12 | 2007-06-14 | Qiagen North American Holdings, Inc. | Compositions and methods for using a solid support to purify RNA |
| DE60230788D1 (de) | 2001-11-06 | 2009-02-26 | Cortex Biochem Inc | Isolierung und aufreinigung von nukleinsäuren |
| JP3890556B2 (ja) | 2001-11-28 | 2007-03-07 | 富士フイルム株式会社 | 核酸の分離精製方法及び核酸分離精製ユニット |
-
2002
- 2002-07-19 JP JP2002210833A patent/JP3983125B2/ja not_active Expired - Fee Related
-
2003
- 2003-07-18 DE DE60300356T patent/DE60300356T2/de not_active Expired - Lifetime
- 2003-07-18 AT AT03015676T patent/ATE290076T1/de not_active IP Right Cessation
- 2003-07-18 EP EP03015676A patent/EP1382677B1/de not_active Expired - Lifetime
- 2003-07-18 US US10/621,412 patent/US7252939B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ATE290076T1 (de) | 2005-03-15 |
| DE60300356T2 (de) | 2006-04-06 |
| JP2004049107A (ja) | 2004-02-19 |
| US20040058370A1 (en) | 2004-03-25 |
| EP1382677B1 (de) | 2005-03-02 |
| EP1382677A1 (de) | 2004-01-21 |
| US7252939B2 (en) | 2007-08-07 |
| JP3983125B2 (ja) | 2007-09-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: FUJIFILM CORP., TOKIO/TOKYO, JP |