ATE427991T1 - Verfahren zur in vivo quantifizierung von nukleinsauren - Google Patents

Verfahren zur in vivo quantifizierung von nukleinsauren

Info

Publication number
ATE427991T1
ATE427991T1 AT03729485T AT03729485T ATE427991T1 AT E427991 T1 ATE427991 T1 AT E427991T1 AT 03729485 T AT03729485 T AT 03729485T AT 03729485 T AT03729485 T AT 03729485T AT E427991 T1 ATE427991 T1 AT E427991T1
Authority
AT
Austria
Prior art keywords
nucleic acids
biological sample
precipitate
present
quantification
Prior art date
Application number
AT03729485T
Other languages
English (en)
Inventor
Patrick Stordeur
Michel Goldman
Original Assignee
Univ Bruxelles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8185856&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE427991(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Bruxelles filed Critical Univ Bruxelles
Application granted granted Critical
Publication of ATE427991T1 publication Critical patent/ATE427991T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT03729485T 2002-01-18 2003-01-20 Verfahren zur in vivo quantifizierung von nukleinsauren ATE427991T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02447009A EP1329506A1 (de) 2002-01-18 2002-01-18 Verfahren zur Quantifizierung vom RNA Niveau in vivo

Publications (1)

Publication Number Publication Date
ATE427991T1 true ATE427991T1 (de) 2009-04-15

Family

ID=8185856

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03729485T ATE427991T1 (de) 2002-01-18 2003-01-20 Verfahren zur in vivo quantifizierung von nukleinsauren

Country Status (8)

Country Link
US (1) US20050153292A1 (de)
EP (2) EP1329506A1 (de)
JP (1) JP2005514065A (de)
AT (1) ATE427991T1 (de)
AU (1) AU2003235581A1 (de)
CA (1) CA2473613A1 (de)
DE (1) DE60327048D1 (de)
WO (1) WO2003060119A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10208005A1 (de) * 2002-02-26 2003-09-04 Qiagen Gmbh Verfahren zur Änderung der Transkriptkonzentration in ribonukleinsäurehaltigen biologischen Proben
FR2874026B1 (fr) * 2004-08-04 2006-11-17 Centre Nat Rech Scient Cnrse QUANTIFICATION DES ARNm DE PROTEINES D'INTERET AU MOYEN D'UNE REACTION RT-PCR EN TEMPS REEL
AU2005305012C1 (en) * 2004-11-05 2012-07-19 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
WO2007133495A1 (en) * 2006-05-10 2007-11-22 Human Genome Sciences, Inc. Mixture for decreasing protein interference
JP5073967B2 (ja) * 2006-05-30 2012-11-14 株式会社日立製作所 単一細胞の遺伝子発現定量方法
WO2009081854A1 (ja) * 2007-12-21 2009-07-02 University Of Toyama アレルギー性疾患のバイオマーカーおよびその利用
DK2742152T3 (en) 2011-08-12 2017-07-31 Qiagen Gmbh PROCEDURE FOR ISOLATING NUCLEIC ACIDS
JP2014176303A (ja) * 2013-03-13 2014-09-25 Seiko Epson Corp cDNAの合成方法
CN110191960A (zh) 2016-11-08 2019-08-30 克维拉公司 进行核酸稳定和分离的方法
IL298468A (en) * 2020-05-29 2023-01-01 Univ Rockefeller A method and system for isolating RNA from self-collected samples in a small volume

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5010183A (en) 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
JP3615545B2 (ja) 1993-02-01 2005-02-02 キアゲン・エヌ・ブイ 第四級アンモニウム塩界面活性剤及びそのrnaの単離剤
US6664107B1 (en) * 1993-05-26 2003-12-16 Ontario Cancer Institute, University Health Network CD45 disrupted nucleic acid
US6190857B1 (en) * 1997-03-24 2001-02-20 Urocor, Inc. Diagnosis of disease state using MRNA profiles in peripheral leukocytes
US5906744A (en) * 1997-04-30 1999-05-25 Becton Dickinson And Company Tube for preparing a plasma specimen for diagnostic assays and method of making thereof
CA2326827A1 (en) * 1998-05-05 1999-11-11 Gene Logic, Inc. A process to study changes in gene expression in t lymphocytes
FR2798673B1 (fr) * 1999-09-16 2004-05-28 Exonhit Therapeutics Sa Methodes et compositions pour la detection d'evenements pathologiques
US6271002B1 (en) * 1999-10-04 2001-08-07 Rosetta Inpharmatics, Inc. RNA amplification method
DE10031236A1 (de) * 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
WO2002086113A2 (en) * 2001-04-24 2002-10-31 Isis Innovation Ltd Enzyme and snp marker for disease

Also Published As

Publication number Publication date
AU2003235581A1 (en) 2003-07-30
DE60327048D1 (de) 2009-05-20
EP1329506A1 (de) 2003-07-23
JP2005514065A (ja) 2005-05-19
US20050153292A1 (en) 2005-07-14
CA2473613A1 (en) 2003-07-24
WO2003060119A3 (en) 2004-02-19
WO2003060119A2 (en) 2003-07-24
AU2003235581A8 (en) 2003-07-30
EP1468089A2 (de) 2004-10-20
EP1468089B1 (de) 2009-04-08

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