ATE298804T1 - Verfahren zur nachweis von nukleinsäuren - Google Patents

Verfahren zur nachweis von nukleinsäuren

Info

Publication number
ATE298804T1
ATE298804T1 AT02738330T AT02738330T ATE298804T1 AT E298804 T1 ATE298804 T1 AT E298804T1 AT 02738330 T AT02738330 T AT 02738330T AT 02738330 T AT02738330 T AT 02738330T AT E298804 T1 ATE298804 T1 AT E298804T1
Authority
AT
Austria
Prior art keywords
nucleic acid
sample
sequence
hybridise
label
Prior art date
Application number
AT02738330T
Other languages
English (en)
Inventor
Martin Alan Lee
Original Assignee
Secr Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Secr Defence filed Critical Secr Defence
Application granted granted Critical
Publication of ATE298804T1 publication Critical patent/ATE298804T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Eye Examination Apparatus (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Geophysics And Detection Of Objects (AREA)
AT02738330T 2001-05-25 2002-05-24 Verfahren zur nachweis von nukleinsäuren ATE298804T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0112868.5A GB0112868D0 (en) 2001-05-25 2001-05-25 Detection system
PCT/GB2002/002443 WO2002097132A2 (en) 2001-05-25 2002-05-24 Nucleic acid detection method

Publications (1)

Publication Number Publication Date
ATE298804T1 true ATE298804T1 (de) 2005-07-15

Family

ID=9915364

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02738330T ATE298804T1 (de) 2001-05-25 2002-05-24 Verfahren zur nachweis von nukleinsäuren

Country Status (15)

Country Link
US (2) US7700275B2 (de)
EP (1) EP1390542B1 (de)
JP (1) JP2004532044A (de)
KR (1) KR20040024556A (de)
CN (1) CN1304596C (de)
AT (1) ATE298804T1 (de)
AU (1) AU2002311414B2 (de)
CA (1) CA2448211C (de)
DE (1) DE60204874T2 (de)
DK (1) DK1390542T3 (de)
ES (1) ES2240753T3 (de)
GB (1) GB0112868D0 (de)
HK (1) HK1068651A1 (de)
PT (1) PT1390542E (de)
WO (1) WO2002097132A2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
GB0223563D0 (en) * 2002-10-10 2002-11-20 Secr Defence Detection system
ES2359322T3 (es) * 2004-10-18 2011-05-20 Brandeis University Procedimiento para la amplificación de ácidos nucleicos.
KR100764559B1 (ko) * 2005-05-09 2007-10-08 한국표준과학연구원 Ｅｐｓｐｓ 유전자 검색에 활용할 수 있는 ｆｒｅｔ형광검출용 프로브
WO2006121277A1 (en) * 2005-05-09 2006-11-16 Korea Research Institute Of Standards And Science Fret probes for fluorescent detection of the epsps gene
CN100420943C (zh) * 2005-06-23 2008-09-24 李卫 Dna分子内杂交-聚丙烯酰胺凝胶电泳方法
US8968700B2 (en) * 2005-08-11 2015-03-03 The Board Of Trustees Of The Leland Stanford Junior University Imaging of protease activity in live cells using activity based probes
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
CN100526461C (zh) * 2006-07-10 2009-08-12 中国农业科学院棉花研究所 获得植物内含子序列扩增多态性的方法及其专用引物
JP2009044967A (ja) * 2007-08-14 2009-03-05 Sony Corp 二本鎖核酸の形成に係る情報を得る方法
US8658366B2 (en) 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
GB201007868D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Sequence detection assay
WO2012118715A2 (en) 2011-02-28 2012-09-07 The Board Of Trustees Of Leland Stanford Junior University Non-peptidic quenched fluorescent imaging probes
DE102011117520A1 (de) * 2011-11-03 2013-05-08 Frank Schleifenbaum Sensorsystem für nicht intrusive Druckmessungen
EP4218829A3 (de) 2013-03-15 2023-08-16 The Board of Trustees of the Leland Stanford Junior University Aktivitätsbasierte sondenverbindungen, zusammensetzungen und verfahren zur verwendung
EP3347714B1 (de) * 2015-09-10 2021-03-10 InSilixa Inc. Verfahren für quantitative multiplex-nukleinsäureamplifikation
AU2017382460A1 (en) 2016-12-23 2019-05-02 The Board Of Trustees Of The Leland Stanford Junior University Activity-based probe compounds, compositions, and methods of use
KR102669535B1 (ko) 2017-03-30 2024-05-24 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 생체 내 이미징을 위한 프로테아제 활성화 조영제

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1533151A (fr) * 1962-05-18 1968-07-19 Rhone Poulenc Sa Nouvel antibiotique et sa préparation
US4012284A (en) * 1962-11-16 1977-03-15 Societa' Farmaceutici Italia, S.p.A. Process of preparation of antibiotic F.I. 1762 derivatives
US3183157A (en) * 1963-02-01 1965-05-11 Upjohn Co Antibiotic nogalamycin and method of producing
US4197249A (en) * 1977-08-15 1980-04-08 American Cyanamid Company 1,4-Bis(substituted-amino)-5,8-dihydroxyanthraquinones and leuco bases thereof
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
CA1219824A (en) 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
US5208323A (en) * 1989-08-10 1993-05-04 Universite Laval Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent
HU201626B (en) 1989-09-05 1990-11-28 Magyar Kabel Muevek Device for making surface layer on work pieces moving longitudinally particularly by use of dusty integumentary material in cable industry
GB8923075D0 (en) * 1989-10-13 1989-11-29 Patterson Laurence H Anti-cancer compounds
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5849486A (en) 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US5567583A (en) * 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
ES2082256T3 (es) 1992-03-23 1996-03-16 Hoffmann La Roche Metodo de deteccion del adn.
JP3696238B2 (ja) * 1993-08-30 2005-09-14 プロメガ・コーポレイシヨン 核酸精製用組成物及び方法
WO1995008642A1 (en) 1993-09-23 1995-03-30 Zeneca Limited Nucleic acid detection with energy transfer
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
DE69527125T2 (de) * 1994-11-09 2003-02-27 Hitachi, Ltd. Verfahren und Vorrichtung zur DNS Analyse
US5858397A (en) * 1995-10-11 1999-01-12 University Of British Columbia Liposomal formulations of mitoxantrone
CA2255774C (en) * 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
CA2257109C (en) 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9716052D0 (en) 1996-12-06 1997-10-01 Secr Defence Reaction vessels
EP1009802B1 (de) * 1997-02-12 2004-08-11 Eugene Y. Chan Verfahren zur analyse von polymeren
US6403311B1 (en) * 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
GB9725237D0 (en) 1997-11-29 1998-01-28 Secr Defence Amplification system
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
GB9918237D0 (en) * 1999-08-04 1999-10-06 Secr Defence Detection system
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
KR100386606B1 (ko) * 2001-02-03 2003-06-02 엘지전자 주식회사 Ｄｎａ 검출 방법 및 그 장치
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
KR100459394B1 (ko) * 2001-10-30 2004-12-03 엘지전자 주식회사 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법
WO2003046208A2 (en) * 2001-11-28 2003-06-05 Mj Bioworks Incorporated Parallel polymorphism scoring by amplification and error correction
DE60229890D1 (de) * 2002-09-30 2008-12-24 Hoffmann La Roche Oligonukleotide zur genotypisierung des thymidylat-synthase gens
GB0223563D0 (en) 2002-10-10 2002-11-20 Secr Defence Detection system
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
WO2005047521A2 (en) * 2003-11-10 2005-05-26 Investigen, Inc. Methods of preparing nucleic acid for detection

Also Published As

Publication number Publication date
US20040241679A1 (en) 2004-12-02
US7700275B2 (en) 2010-04-20
EP1390542B1 (de) 2005-06-29
GB0112868D0 (en) 2001-07-18
CN1527885A (zh) 2004-09-08
CN1304596C (zh) 2007-03-14
EP1390542A2 (de) 2004-02-25
PT1390542E (pt) 2005-11-30
WO2002097132A2 (en) 2002-12-05
DE60204874D1 (de) 2005-08-04
DE60204874T2 (de) 2006-04-27
HK1068651A1 (en) 2005-04-29
CA2448211A1 (en) 2002-12-05
ES2240753T3 (es) 2005-10-16
KR20040024556A (ko) 2004-03-20
DK1390542T3 (da) 2005-08-08
US20100227326A1 (en) 2010-09-09
CA2448211C (en) 2010-12-07
JP2004532044A (ja) 2004-10-21
AU2002311414B2 (en) 2006-11-09
WO2002097132A3 (en) 2003-09-12

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