WO2003004686A3 - Fluorescence-based assay for the detection of specific nucleic acids using photon counting - Google Patents
Fluorescence-based assay for the detection of specific nucleic acids using photon counting Download PDFInfo
- Publication number
- WO2003004686A3 WO2003004686A3 PCT/US2002/021353 US0221353W WO03004686A3 WO 2003004686 A3 WO2003004686 A3 WO 2003004686A3 US 0221353 W US0221353 W US 0221353W WO 03004686 A3 WO03004686 A3 WO 03004686A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- nucleic acids
- fluorescent
- labeled
- assay
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present assay is directed to a sensitive assay for detecting the presence of small amounts of a specific nThe present assay is directed to a sensitive assay for detecting the presence of small amounts of a specific nucleic acid sequence or sequences in a mixture of sample nucleic acids using photon counting, wherein the sample nucleic acids have not been amplified by polymerase chain reaction prior to assay or further separated from the mixture of sample nucleic acids once the mixture has been isolated from its original source. In one aspect, the present assay comprises binding a mixture of sample nucleic acids to a solid matrix; contacting the matrix-bound sample nucleic acids with a fluorescent-labeled nucleic acid probe; washing the matrix-bound sample nucleic acids under stringent conditions to remove any free or non-specifically bound fluorescent-labeled nucleic acid probe; removing the specifically bound fluorescent-labeled nucleic acid probe; and detecting the presence of the specifically bound fluorescent-labeled nucleic acid probe using photon counting, wherein the presence of fluorescent-labeled probe is indicative of the presence of a target nucleic acid or nucleic acids in the biological sample. In another aspect, the assay includes a polypeptide-tagged nucleic acid probe and a fluorescent-labeled antibody that is specific for the polypeptide tag of the nucleic acid probe, wherein the fluorescent-labeled antibody is detected using photon counting and indicative of the presence of a target nucleic acid or nucleic acids in the biological sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2002354805A AU2002354805A1 (en) | 2001-07-03 | 2002-07-03 | Fluorescence-based assay for the detection of specific nucleic acids using photon counting |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US30289601P | 2001-07-03 | 2001-07-03 | |
| US60/302,896 | 2001-07-03 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2003004686A2 WO2003004686A2 (en) | 2003-01-16 |
| WO2003004686A3 true WO2003004686A3 (en) | 2003-08-21 |
Family
ID=23169671
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2002/021353 WO2003004686A2 (en) | 2001-07-03 | 2002-07-03 | Fluorescence-based assay for the detection of specific nucleic acids using photon counting |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU2002354805A1 (en) |
| WO (1) | WO2003004686A2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080044919A1 (en) * | 2004-08-13 | 2008-02-21 | Dowben Robert M | Analyte Detection Using Time-Resolved Photon Counting Fluorescence |
| CN108463558A (en) * | 2016-01-22 | 2018-08-28 | 爱科来株式会社 | Target analysis method and target analysis chip |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4824776A (en) * | 1985-07-25 | 1989-04-25 | Molecular Biosystems, Inc. | Method for increasing the sensitivity of nucleic acid hybridization assays |
| US5262526A (en) * | 1990-12-21 | 1993-11-16 | Dojindo Laboratories | Fluorescent compound, complex, reagent, and specific binding assay employing said reagent |
| US5714331A (en) * | 1991-05-24 | 1998-02-03 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility |
| US5914228A (en) * | 1991-09-12 | 1999-06-22 | Cedars-Sinai Medical Center | Direct detection of hepatitis C virus RNA |
-
2002
- 2002-07-03 AU AU2002354805A patent/AU2002354805A1/en not_active Abandoned
- 2002-07-03 WO PCT/US2002/021353 patent/WO2003004686A2/en not_active Application Discontinuation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4824776A (en) * | 1985-07-25 | 1989-04-25 | Molecular Biosystems, Inc. | Method for increasing the sensitivity of nucleic acid hybridization assays |
| US5262526A (en) * | 1990-12-21 | 1993-11-16 | Dojindo Laboratories | Fluorescent compound, complex, reagent, and specific binding assay employing said reagent |
| US5714331A (en) * | 1991-05-24 | 1998-02-03 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility |
| US5914228A (en) * | 1991-09-12 | 1999-06-22 | Cedars-Sinai Medical Center | Direct detection of hepatitis C virus RNA |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002354805A1 (en) | 2003-01-21 |
| WO2003004686A2 (en) | 2003-01-16 |
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