DE60204874D1 - Verfahren zur nachweis von nukleinsäuren - Google Patents

Verfahren zur nachweis von nukleinsäuren

Info

Publication number
DE60204874D1
DE60204874D1 DE60204874T DE60204874T DE60204874D1 DE 60204874 D1 DE60204874 D1 DE 60204874D1 DE 60204874 T DE60204874 T DE 60204874T DE 60204874 T DE60204874 T DE 60204874T DE 60204874 D1 DE60204874 D1 DE 60204874D1
Authority
DE
Germany
Prior art keywords
nucleic acid
sample
sequence
hybridise
label
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60204874T
Other languages
English (en)
Other versions
DE60204874T2 (de
Inventor
Martin Alan Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Publication of DE60204874D1 publication Critical patent/DE60204874D1/de
Application granted granted Critical
Publication of DE60204874T2 publication Critical patent/DE60204874T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
DE60204874T 2001-05-25 2002-05-24 Verfahren zur nachweis von nukleinsäuren Expired - Lifetime DE60204874T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0112868.5A GB0112868D0 (en) 2001-05-25 2001-05-25 Detection system
GB0112868 2001-05-25
PCT/GB2002/002443 WO2002097132A2 (en) 2001-05-25 2002-05-24 Nucleic acid detection method

Publications (2)

Publication Number Publication Date
DE60204874D1 true DE60204874D1 (de) 2005-08-04
DE60204874T2 DE60204874T2 (de) 2006-04-27

Family

ID=9915364

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60204874T Expired - Lifetime DE60204874T2 (de) 2001-05-25 2002-05-24 Verfahren zur nachweis von nukleinsäuren

Country Status (15)

Country Link
US (2) US7700275B2 (de)
EP (1) EP1390542B1 (de)
JP (1) JP2004532044A (de)
KR (1) KR20040024556A (de)
CN (1) CN1304596C (de)
AT (1) ATE298804T1 (de)
AU (1) AU2002311414B2 (de)
CA (1) CA2448211C (de)
DE (1) DE60204874T2 (de)
DK (1) DK1390542T3 (de)
ES (1) ES2240753T3 (de)
GB (1) GB0112868D0 (de)
HK (1) HK1068651A1 (de)
PT (1) PT1390542E (de)
WO (1) WO2002097132A2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
GB0223563D0 (en) * 2002-10-10 2002-11-20 Secr Defence Detection system
EP2927238B1 (de) 2004-10-18 2018-01-17 Brandeis University Verfahren zur LATE Amplifikation und Sequenzierung von Nukleinsäuren
WO2006121277A1 (en) * 2005-05-09 2006-11-16 Korea Research Institute Of Standards And Science Fret probes for fluorescent detection of the epsps gene
KR100764559B1 (ko) * 2005-05-09 2007-10-08 한국표준과학연구원 Ｅｐｓｐｓ 유전자 검색에 활용할 수 있는 ｆｒｅｔ형광검출용 프로브
CN100420943C (zh) * 2005-06-23 2008-09-24 李卫 Dna分子内杂交-聚丙烯酰胺凝胶电泳方法
US8968700B2 (en) * 2005-08-11 2015-03-03 The Board Of Trustees Of The Leland Stanford Junior University Imaging of protease activity in live cells using activity based probes
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
CN100526461C (zh) * 2006-07-10 2009-08-12 中国农业科学院棉花研究所 获得植物内含子序列扩增多态性的方法及其专用引物
JP2009044967A (ja) * 2007-08-14 2009-03-05 Sony Corp 二本鎖核酸の形成に係る情報を得る方法
US8658366B2 (en) * 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
GB201007868D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Sequence detection assay
WO2012118715A2 (en) 2011-02-28 2012-09-07 The Board Of Trustees Of Leland Stanford Junior University Non-peptidic quenched fluorescent imaging probes
DE102011117520A1 (de) * 2011-11-03 2013-05-08 Frank Schleifenbaum Sensorsystem für nicht intrusive Druckmessungen
EP4218829A3 (de) 2013-03-15 2023-08-16 The Board of Trustees of the Leland Stanford Junior University Aktivitätsbasierte sondenverbindungen, zusammensetzungen und verfahren zur verwendung
EP3859333A1 (de) * 2015-09-10 2021-08-04 InSilixa, Inc. Systeme für quantitative multiplex-nukleinsäureamplifikation
AU2017382460A1 (en) 2016-12-23 2019-05-02 The Board Of Trustees Of The Leland Stanford Junior University Activity-based probe compounds, compositions, and methods of use
EP3601261A4 (de) * 2017-03-30 2021-07-14 The Board of Trustees of the Leland Stanford Junior University Proteaseaktivierte kontrastmittel zur in-vivo-bildgebung

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1533151A (fr) * 1962-05-18 1968-07-19 Rhone Poulenc Sa Nouvel antibiotique et sa préparation
US4012284A (en) * 1962-11-16 1977-03-15 Societa' Farmaceutici Italia, S.p.A. Process of preparation of antibiotic F.I. 1762 derivatives
US3183157A (en) * 1963-02-01 1965-05-11 Upjohn Co Antibiotic nogalamycin and method of producing
US4197249A (en) * 1977-08-15 1980-04-08 American Cyanamid Company 1,4-Bis(substituted-amino)-5,8-dihydroxyanthraquinones and leuco bases thereof
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
CA1219824A (en) 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
US5208323A (en) * 1989-08-10 1993-05-04 Universite Laval Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent
HU201626B (en) 1989-09-05 1990-11-28 Magyar Kabel Muevek Device for making surface layer on work pieces moving longitudinally particularly by use of dusty integumentary material in cable industry
GB8923075D0 (en) * 1989-10-13 1989-11-29 Patterson Laurence H Anti-cancer compounds
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5849486A (en) 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US5567583A (en) * 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
EP0566751B1 (de) 1992-03-23 1996-01-10 F. Hoffmann-La Roche Ag Verfahren zum DNS-Nachweis
ATE256735T1 (de) * 1993-08-30 2004-01-15 Promega Corp Zusammensetzungen und verfahren zur reinigung von nukleinsäuren
WO1995008642A1 (en) 1993-09-23 1995-03-30 Zeneca Limited Nucleic acid detection with energy transfer
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US6106777A (en) * 1994-11-09 2000-08-22 Hitachi, Ltd. DNA analyzing method and device therefor
US5858397A (en) * 1995-10-11 1999-01-12 University Of British Columbia Liposomal formulations of mitoxantrone
EP1736554B1 (de) * 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Bestimmung von Unterschieden in der Nukleinsäuresequenz mittels einer Kombination von Ligasebestimmung und Polymerasekettenreaktion
ATE428801T1 (de) 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9716052D0 (en) 1996-12-06 1997-10-01 Secr Defence Reaction vessels
DE69825601T2 (de) * 1997-02-12 2005-04-28 Chan, Eugene Y, Brookline Verfahren zur analyse von polymeren
US6403311B1 (en) * 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
GB9725237D0 (en) 1997-11-29 1998-01-28 Secr Defence Amplification system
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
GB9918237D0 (en) * 1999-08-04 1999-10-06 Secr Defence Detection system
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
KR100386606B1 (ko) * 2001-02-03 2003-06-02 엘지전자 주식회사 Ｄｎａ 검출 방법 및 그 장치
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
KR100459394B1 (ko) * 2001-10-30 2004-12-03 엘지전자 주식회사 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법
US7163790B2 (en) * 2001-11-28 2007-01-16 Bio-Rad Laboratories, Inc. Parallel polymorphism scoring by amplification and error correction
WO2004031408A1 (en) * 2002-09-30 2004-04-15 F.Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
GB0223563D0 (en) * 2002-10-10 2002-11-20 Secr Defence Detection system
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
JP2007512811A (ja) * 2003-11-10 2007-05-24 インベスチゲン， インコーポレイテッド 検出のための核酸を調製する方法

Also Published As

Publication number Publication date
KR20040024556A (ko) 2004-03-20
AU2002311414B2 (en) 2006-11-09
DK1390542T3 (da) 2005-08-08
CA2448211C (en) 2010-12-07
ES2240753T3 (es) 2005-10-16
CN1527885A (zh) 2004-09-08
WO2002097132A3 (en) 2003-09-12
CA2448211A1 (en) 2002-12-05
EP1390542A2 (de) 2004-02-25
HK1068651A1 (en) 2005-04-29
ATE298804T1 (de) 2005-07-15
US7700275B2 (en) 2010-04-20
JP2004532044A (ja) 2004-10-21
GB0112868D0 (en) 2001-07-18
US20040241679A1 (en) 2004-12-02
WO2002097132A2 (en) 2002-12-05
EP1390542B1 (de) 2005-06-29
US20100227326A1 (en) 2010-09-09
CN1304596C (zh) 2007-03-14
PT1390542E (pt) 2005-11-30
DE60204874T2 (de) 2006-04-27

Similar Documents

Publication Publication Date Title
HK1068651A1 (en) Detection system
DE69828908D1 (de) Fluorimetrisches system zum nachweis von nucleinsäuren
ATE204333T1 (de) Verfahren zur dämpfung der fluoreszenz von fluoreszenzmarkierten oligonukleotiden in lösung
ATE388402T1 (de) Verfahren zur erkennung und zum assay von nukleinsäuresequenzen
DE69937447D1 (de) Verfahren zur erkennung von nukleinsäuren
KR970028548A (ko) 핵산의 형광 편광도 검출방법
WO2002090572A3 (en) Nucleic acid detection in pooled samples
WO2004071155A3 (en) Solid phase sequencing
WO2003020984A3 (en) Terminal-phosphate-labeled nucleotides and methods of use
ATE360708T1 (de) Test-streife-probesystem und verfahren zur nachweis von spezifischen nukleinsäuresequenzen
DE60327345D1 (de) Nachweissystem
RU2265058C2 (ru) Способ выявления в образце нуклеиновой кислоты-мишени (варианты)
ATE440968T1 (de) Verfahren zur diagnostizierung von aspirinunverträglichkeit
WO2011114142A3 (en) Sequence detection assay
ATE455864T1 (de) Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen
WO2001011078A8 (en) Amplification method for detection of target nucleic acids involving fluorescence energy transfer
WO2005017488A3 (en) Method and system for identifying biological entities in biological and environmental samples
ATE347618T1 (de) Verfahren zur amplifikation von nukleinsäuren, das mittel zur strangtrennung und universelle sequenzmarker bereitstellt
EP1221466A3 (de) Nachweisreagenzien sowie Nachweisverfahren für doppelsträngige Nucleinsäure
DE50204689D1 (de) Verfahren zum nachweis von gram-positiven bakterien
WO2003004686A3 (en) Fluorescence-based assay for the detection of specific nucleic acids using photon counting
JP2019154396A (ja) 核酸検出方法、並びに、それに用いる標的核酸検出用プローブ、及び標的核酸検出用デバイス
EP1679381A1 (de) Verfahren zur isomeren Primerverlängerung und Reagenziensatz zum Nachweis und zur Quantifizierung von spezifischen Nukleinsäuren

Legal Events

Date Code Title Description
8364 No opposition during term of opposition