LT2748179T - Baltymų gryninimas anijonų mainų chromatografija - Google Patents

Baltymų gryninimas anijonų mainų chromatografija

Info

Publication number
LT2748179T
LT2748179T LTEPPCT/EP2012/070257T LT12070257T LT2748179T LT 2748179 T LT2748179 T LT 2748179T LT 12070257 T LT12070257 T LT 12070257T LT 2748179 T LT2748179 T LT 2748179T
Authority
LT
Lithuania
Prior art keywords
anion exchange
exchange chromatography
protein purification
purification
protein
Prior art date
Application number
LTEPPCT/EP2012/070257T
Other languages
English (en)
Inventor
Artur Mitterer
Meinhard Hasslacher
Christian Fiedler
Original Assignee
Takeda Pharmaceutical Company Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Pharmaceutical Company Limited filed Critical Takeda Pharmaceutical Company Limited
Publication of LT2748179T publication Critical patent/LT2748179T/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
LTEPPCT/EP2012/070257T 2011-10-14 2012-10-12 Baltymų gryninimas anijonų mainų chromatografija LT2748179T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161547579P 2011-10-14 2011-10-14
PCT/EP2012/070257 WO2013053887A1 (en) 2011-10-14 2012-10-12 Protein purification by anion exchange chromatography

Publications (1)

Publication Number Publication Date
LT2748179T true LT2748179T (lt) 2022-04-11

Family

ID=47010608

Family Applications (1)

Application Number Title Priority Date Filing Date
LTEPPCT/EP2012/070257T LT2748179T (lt) 2011-10-14 2012-10-12 Baltymų gryninimas anijonų mainų chromatografija

Country Status (9)

Country Link
US (1) US10378004B2 (lt)
EP (1) EP2748179B1 (lt)
JP (2) JP6236008B2 (lt)
AU (1) AU2012322948B2 (lt)
ES (1) ES2908920T3 (lt)
HK (1) HK1198588A1 (lt)
LT (1) LT2748179T (lt)
PL (1) PL2748179T3 (lt)
WO (1) WO2013053887A1 (lt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103025757A (zh) 2010-04-29 2013-04-03 巴克斯特国际有限公司 二价阳离子结合蛋白在阴离子交换树脂上的纯化方法
WO2013053888A1 (en) * 2011-10-14 2013-04-18 Baxter International Inc. Protein purification by anion exchange chromatography
JP6236008B2 (ja) 2011-10-14 2017-11-22 バクスアルタ ゲーエムベーハー アニオン交換クロマトグラフィーによるタンパク質の精製
SI2970376T1 (sl) * 2013-03-15 2018-09-28 Baxalta Incorporated Metoda čiščenja za vitamin k odvisne proteine z anionsko izmenjevalno kromatografijo
TWI684599B (zh) * 2014-08-12 2020-02-11 美商巴克斯歐塔公司 因子ｘ活化
ES2906118T3 (es) * 2016-12-22 2022-04-13 Km Biologics Co Ltd Método cromatográfico para recolectar factor VII de coagulación sanguínea con alto rendimiento
WO2022172284A1 (en) * 2021-02-11 2022-08-18 Gennova Biopharmaceuticals Ltd. Purification of a therapeutic protein

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2045167T5 (es) 1987-10-23 1996-07-01 Centre Regional De Transfusion Preparacion de concentrado de factor ix humano de elevada pureza y de otras proteinas plasmaticas.
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
WO1994005692A1 (en) * 1992-08-27 1994-03-17 Stichting Centraal Laboratorium Van De Bloedtransfusiedienst Van Het Nederlandse Rode Kruis Antibodies specific for a haemostatic protein, their use for isolating intact protein, haemostatic compositions devoid of proteolytic cleavage products of the protein
DE4406515C1 (de) * 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
ES2340008T3 (es) 1997-02-14 2010-05-27 American Red Cross Expresion de factor ix humano activo en tejido mamario de animales transgenicos.
ATE426611T1 (de) 2000-07-21 2009-04-15 Chemo Sero Therapeut Res Inst Verfahren zur reinigung eines kalziumionen- bindenden proteins
US20040106779A1 (en) 2002-12-03 2004-06-03 Bigler Douglas E. Modified factor IX preparation
EP1797122A2 (en) * 2004-09-29 2007-06-20 Novo Nordisk Health Care AG Purification of a drug substance of a factor vii polypeptide by removal of desgla-factor vii polypeptide structures
KR101364001B1 (ko) * 2004-12-23 2014-02-17 노보 노르디스크 헬스 케어 악티엔게젤샤프트 관심있는 비타민 ｋ-의존성 단백질을 포함한 조성물 중의단백질 오염물질의 양의 감소
WO2007026020A1 (en) 2005-09-01 2007-03-08 Novo Nordisk Health Care Ag Purification of coagulation factor vii polypeptides
EP1991257B1 (en) 2006-03-07 2015-05-06 Baxter International Inc. Highly phosphorylated and sulfated recombinant factor ix
FR2901796A1 (fr) 2006-05-31 2007-12-07 Lab Francais Du Fractionnement Procede d'extraction d'une ou de plusieurs proteines presentes dans du lait
FR2901707B1 (fr) 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
WO2008104372A1 (en) * 2007-02-28 2008-09-04 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules
JP5984676B2 (ja) 2009-12-18 2016-09-06 シーエスエル、リミテッド ポリペプチドを精製する方法
CN103025757A (zh) * 2010-04-29 2013-04-03 巴克斯特国际有限公司 二价阳离子结合蛋白在阴离子交换树脂上的纯化方法
JP6236008B2 (ja) 2011-10-14 2017-11-22 バクスアルタ ゲーエムベーハー アニオン交換クロマトグラフィーによるタンパク質の精製
WO2013053888A1 (en) 2011-10-14 2013-04-18 Baxter International Inc. Protein purification by anion exchange chromatography

Also Published As

Publication number Publication date
JP6236008B2 (ja) 2017-11-22
EP2748179A1 (en) 2014-07-02
US10378004B2 (en) 2019-08-13
US20140302591A1 (en) 2014-10-09
JP2017160248A (ja) 2017-09-14
PL2748179T3 (pl) 2022-04-11
AU2012322948A1 (en) 2013-05-16
JP6820232B2 (ja) 2021-01-27
ES2908920T3 (es) 2022-05-04
HK1198588A1 (en) 2015-04-30
EP2748179B1 (en) 2021-12-29
JP2014530230A (ja) 2014-11-17
WO2013053887A1 (en) 2013-04-18
AU2012322948B2 (en) 2014-11-06

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