AU2001272736A1 - Method of purifying calcium ion-binding protein - Google Patents

Method of purifying calcium ion-binding protein

Info

Publication number
AU2001272736A1
AU2001272736A1 AU2001272736A AU7273601A AU2001272736A1 AU 2001272736 A1 AU2001272736 A1 AU 2001272736A1 AU 2001272736 A AU2001272736 A AU 2001272736A AU 7273601 A AU7273601 A AU 7273601A AU 2001272736 A1 AU2001272736 A1 AU 2001272736A1
Authority
AU
Australia
Prior art keywords
calcium ion
binding protein
protein
purifying
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001272736A
Inventor
Shinichi Furukawa
Kazuhiro Komatsu
Satoshi Koyanagi
Hiroshi Mizokami
Tatsufumi Onchi
Keishin Sugawara
Hideo Yoshizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemo Sero Therapeutic Research Institute Kaketsuken
Kowa Co Ltd
Original Assignee
Chemo Sero Therapeutic Research Institute Kaketsuken
Kowa Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemo Sero Therapeutic Research Institute Kaketsuken, Kowa Co Ltd filed Critical Chemo Sero Therapeutic Research Institute Kaketsuken
Publication of AU2001272736A1 publication Critical patent/AU2001272736A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4728—Calcium binding proteins, e.g. calmodulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)

Abstract

The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
AU2001272736A 2000-07-21 2001-07-18 Method of purifying calcium ion-binding protein Abandoned AU2001272736A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000-220600 2000-07-21
JP2000220600 2000-07-21
PCT/JP2001/006209 WO2002008249A1 (en) 2000-07-21 2001-07-18 Method of purifying calcium ion-binding protein

Publications (1)

Publication Number Publication Date
AU2001272736A1 true AU2001272736A1 (en) 2002-02-05

Family

ID=18715177

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001272736A Abandoned AU2001272736A1 (en) 2000-07-21 2001-07-18 Method of purifying calcium ion-binding protein

Country Status (9)

Country Link
US (2) US6869934B2 (en)
EP (1) EP1306383B1 (en)
KR (1) KR100841599B1 (en)
CN (1) CN1250567C (en)
AT (1) ATE426611T1 (en)
AU (1) AU2001272736A1 (en)
CA (1) CA2385371A1 (en)
DE (1) DE60138111D1 (en)
WO (1) WO2002008249A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106967150B (en) 2010-04-29 2020-12-04 百深公司 Purification of divalent cation binding proteins on anion exchange resins
CN102382168A (en) * 2010-09-03 2012-03-21 中国科学院过程工程研究所 Ion exchange chromatography separation and purification method for efficiently purifying metal ion binding proteins
US10378004B2 (en) 2011-10-14 2019-08-13 Baxalta GmbH Protein purification by anion exchange chromatography
ES2700933T3 (en) * 2011-10-14 2019-02-20 Baxalta GmbH Protein purification by anion exchange chromatography
ES2682928T3 (en) 2013-03-15 2018-09-24 Baxalta Incorporated Purification method for vitamin K dependent proteins by anion exchange chromatography
JP6910033B2 (en) * 2016-08-01 2021-07-28 株式会社カネカ Adsorbent for calciprotein particles, adsorption removal system and its usage

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1265446A (en) 1985-09-30 1990-02-06 Masahiro Maki Anticoagulating substance, process for preparing same and anticoagulant comprising same as an effective component
JP2660514B2 (en) 1987-02-20 1997-10-08 興和株式会社 Polypeptide having anticoagulant action
US4981952A (en) 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
ES2045669T3 (en) * 1989-07-15 1994-01-16 Boehringer Ingelheim Int PROCEDURE FOR THE PURIFICATION OF ANEXINS.
JP2743207B2 (en) 1990-01-25 1998-04-22 財団法人化学及血清療法研究所 Method for producing CPB-I, recombinant plasmid and transformed yeast used for the method
DE4406515C1 (en) 1994-02-28 1995-10-19 Immuno Ag Process for the isolation and purification of vitamin K-dependent proteins
JP3574659B2 (en) * 1994-07-23 2004-10-06 ジャン‐マリー フレイッシネ Method for determining prethrombotic condition
EP0731166A3 (en) * 1995-03-06 1997-12-29 Tonen Corporation Novel calcium-binding proteins
US5976832A (en) * 1995-03-06 1999-11-02 Tonen Corporation DNA encoding novel calcium-binding proteins
US5658877A (en) * 1995-05-18 1997-08-19 Wisconsin Alumni Research Foundation Method to treat endotoxin effects by administration of 33 kilodalton phospholipid binding protein
US6645465B2 (en) * 1999-08-06 2003-11-11 Michigan, University Of The Regents Annexin proteins and autoantibodies as serum markers for cancer

Also Published As

Publication number Publication date
EP1306383A4 (en) 2005-01-26
CA2385371A1 (en) 2002-01-31
CN1392878A (en) 2003-01-22
EP1306383B1 (en) 2009-03-25
KR20020057960A (en) 2002-07-12
EP1306383A1 (en) 2003-05-02
ATE426611T1 (en) 2009-04-15
CN1250567C (en) 2006-04-12
US20050059805A1 (en) 2005-03-17
KR100841599B1 (en) 2008-06-26
WO2002008249A1 (en) 2002-01-31
DE60138111D1 (en) 2009-05-07
US20020169294A1 (en) 2002-11-14
US6869934B2 (en) 2005-03-22

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