DE60213248D1 - Verfahren zur reinigung hochanionischer proteine - Google Patents

Verfahren zur reinigung hochanionischer proteine

Info

Publication number
DE60213248D1
DE60213248D1 DE60213248T DE60213248T DE60213248D1 DE 60213248 D1 DE60213248 D1 DE 60213248D1 DE 60213248 T DE60213248 T DE 60213248T DE 60213248 T DE60213248 T DE 60213248T DE 60213248 D1 DE60213248 D1 DE 60213248D1
Authority
DE
Germany
Prior art keywords
containing molecules
mixture
cleaning high
anionic proteins
complexes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60213248T
Other languages
English (en)
Other versions
DE60213248T2 (de
Inventor
L Coffman
B Foster
Shujun Sun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Institute LLC
Original Assignee
Genetics Institute LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Institute LLC filed Critical Genetics Institute LLC
Publication of DE60213248D1 publication Critical patent/DE60213248D1/de
Application granted granted Critical
Publication of DE60213248T2 publication Critical patent/DE60213248T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/828—Protein A

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60213248T 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine Expired - Lifetime DE60213248T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29640201P 2001-06-05 2001-06-05
US296402P 2001-06-05
PCT/US2002/018081 WO2002098531A1 (en) 2001-06-05 2002-06-05 Methods for purifying highly anionic proteins

Publications (2)

Publication Number Publication Date
DE60213248D1 true DE60213248D1 (de) 2006-08-31
DE60213248T2 DE60213248T2 (de) 2006-11-30

Family

ID=23141860

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60231016T Expired - Lifetime DE60231016D1 (de) 2001-06-05 2002-06-05 Verfahren zur Reinigung stark anionischer Proteine
DE60213248T Expired - Lifetime DE60213248T2 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE60231016T Expired - Lifetime DE60231016D1 (de) 2001-06-05 2002-06-05 Verfahren zur Reinigung stark anionischer Proteine

Country Status (11)

Country Link
US (1) US7223848B2 (de)
EP (2) EP1714982B1 (de)
JP (1) JP4288153B2 (de)
AT (2) ATE421536T1 (de)
AU (1) AU2008203302B2 (de)
CA (1) CA2448788C (de)
DE (2) DE60231016D1 (de)
DK (2) DK1714982T3 (de)
ES (2) ES2321539T3 (de)
NZ (1) NZ530025A (de)
WO (1) WO2002098531A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
DE602006003108D1 (de) * 2005-03-03 2008-11-27 Ajinomoto Kk Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
WO2007075283A2 (en) * 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
CA2636109A1 (en) * 2006-01-06 2007-07-19 Amgen Inc. Methods and systems for isolating target molecules from complex solutions by column-chromatography using eluants containing organic solvents
ES2764222T3 (es) * 2006-09-08 2020-06-02 Wyeth Llc Lavado con arginina en la purificación de proteínas mediante el uso de cromatografía de afinidad
SG10201510384UA (en) 2006-09-13 2016-01-28 Abbvie Inc Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
DK2089429T3 (en) * 2006-10-19 2015-01-12 Janssen Biotech Inc PROCEDURE FOR MANUFACTURING NON-AGGREGATED ANTIBODY-FC DOMAINS
MX2011004200A (es) * 2008-10-20 2011-05-24 Abbott Lab Aislamento y purificacion de anticuerpos usando la cromatografia de afinidad de proteina a.
WO2010048192A2 (en) 2008-10-20 2010-04-29 Abbott Laboratories Viral inactivation during purification of antibodies
WO2010080065A1 (en) * 2009-01-12 2010-07-15 Ge Healthcare Bio-Sciences Ab Affinity chromatography matrix
WO2011064910A1 (ja) * 2009-11-25 2011-06-03 パナソニック株式会社 免疫測定方法
SG10201408384PA (en) 2009-12-18 2015-01-29 Novartis Ag Wash solution and method for affinity chromatography
EP3428176A1 (de) 2011-06-01 2019-01-16 Novartis AG Waschlösung und verfahren für affinitätschromatographie
TWI787710B (zh) * 2020-01-20 2022-12-21 中國大陸商上海藥明生物技術有限公司 用於親和層析的新型清洗緩衝液

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4983722A (en) 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5115101A (en) * 1988-06-08 1992-05-19 Miles Inc. Removal of protein A from antibody preparations
US5464778A (en) 1989-03-08 1995-11-07 Board Of Regents Of The University Of Oklahoma Glycoprotein ligand for P-selectin and methods of use thereof
GB9022547D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Purified immunoglobulin
US5726293A (en) * 1992-10-02 1998-03-10 The General Hospital Corporation Affinity purification methods involving imidazole elution
US6277975B1 (en) 1992-10-23 2001-08-21 Genetics Institute, Inc. Fusions of P-selectin ligand protein and polynucleotides encoding same
US5843707A (en) 1992-10-23 1998-12-01 Genetics Institute, Inc. Nucleic acid encoding a novel P-selectin ligand protein
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
EP1104809A1 (de) 1994-04-09 2001-06-06 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von Alpha-Interferon
US6008328A (en) 1994-10-13 1999-12-28 Amgen Inc. Method for purifying keratinocyte growth factors
SE503424C2 (sv) 1994-11-14 1996-06-10 Pharmacia Ab Process för rening av rekombinant koagulationsfaktor VIII
FR2737730B1 (fr) 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
FR2738009B1 (fr) 1995-08-24 1997-10-31 Centre Nat Rech Scient Procede d'obtention de polysaccharides sulfates
CN1268639C (zh) 1996-11-15 2006-08-09 基因技术股份有限公司 神经营养蛋白的纯化
SI0950067T1 (sl) * 1996-11-27 2007-12-31 Genentech Inc Afinitetno čiščenje polipeptida na proteinskemA matriksu
ES2262340T3 (es) 1998-09-24 2006-11-16 Pharming Intellectual Property Bv Purificacion del fibrinogeno de la leche mediante el uso de cromatografia de intercambio cationico.
IL142717A0 (en) * 1998-10-30 2002-03-10 Genetics Inst Inhibition of differentiation of cytotoxic t-cells by p-selectin ligand (psgl) antagonists
JP2003517580A (ja) 1999-01-21 2003-05-27 メタモーフイクス・インコーポレーテツド 増殖分化因子インヒビター及びそれらの用途
ES2343023T3 (es) * 2000-03-27 2010-07-21 Genetics Institute, Llc Procedimientos para purificar proteinas altamente anionicas.

Also Published As

Publication number Publication date
EP1714982A3 (de) 2006-12-20
JP4288153B2 (ja) 2009-07-01
US7223848B2 (en) 2007-05-29
JP2004537042A (ja) 2004-12-09
AU2008203302A1 (en) 2008-08-14
EP1404428B1 (de) 2006-07-19
AU2008203302B2 (en) 2011-10-27
CA2448788C (en) 2010-08-24
DE60213248T2 (de) 2006-11-30
EP1714982A2 (de) 2006-10-25
ATE333469T1 (de) 2006-08-15
DK1404428T3 (da) 2006-10-30
US20030050450A1 (en) 2003-03-13
DE60231016D1 (de) 2009-03-12
CA2448788A1 (en) 2002-12-12
ES2321539T3 (es) 2009-06-08
EP1404428A1 (de) 2004-04-07
ATE421536T1 (de) 2009-02-15
ES2266535T3 (es) 2007-03-01
DK1714982T3 (da) 2009-05-04
WO2002098531A1 (en) 2002-12-12
EP1404428A4 (de) 2004-12-08
NZ530025A (en) 2005-06-24
EP1714982B1 (de) 2009-01-21

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