DE602006003108D1 - Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin - Google Patents

Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin

Info

Publication number
DE602006003108D1
DE602006003108D1 DE602006003108T DE602006003108T DE602006003108D1 DE 602006003108 D1 DE602006003108 D1 DE 602006003108D1 DE 602006003108 T DE602006003108 T DE 602006003108T DE 602006003108 T DE602006003108 T DE 602006003108T DE 602006003108 D1 DE602006003108 D1 DE 602006003108D1
Authority
DE
Germany
Prior art keywords
arginine
yield
improving
gel filtration
protein purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006003108T
Other languages
English (en)
Inventor
Daisuke Ejima
Ryosuke Yumioka
Tsutomu Arakawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Publication of DE602006003108D1 publication Critical patent/DE602006003108D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factors [FGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5412—IL-6
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
DE602006003108T 2005-03-03 2006-02-23 Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin Active DE602006003108D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US65773505P 2005-03-03 2005-03-03

Publications (1)

Publication Number Publication Date
DE602006003108D1 true DE602006003108D1 (de) 2008-11-27

Family

ID=36406515

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006003108T Active DE602006003108D1 (de) 2005-03-03 2006-02-23 Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin

Country Status (4)

Country Link
US (1) US7501495B2 (de)
EP (1) EP1698637B1 (de)
JP (1) JP4941882B2 (de)
DE (1) DE602006003108D1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
EP1973942B1 (de) * 2005-12-22 2011-02-09 Genentech, Inc. Rekombinante produktion heparinbindender proteine
JP5566104B2 (ja) * 2006-07-14 2014-08-06 ジェネンテック， インコーポレイテッド 組み換えタンパク質の再折り畳み
US8669045B2 (en) * 2007-02-13 2014-03-11 Ajinomoto Co., Inc. Method for inactivating viruses with slightly acidic arginine
JP5168604B2 (ja) * 2008-05-08 2013-03-21 味の素株式会社 タンパク質のリフォールディング方法
EP2360183B1 (de) 2008-12-19 2016-07-27 Takeda Pharmaceutical Company Limited Antikörperreinigungsverfahren mittels "mixed-mode" chromatography unter verwendung einer arginine-haltigen beladungslösung
PL2513134T3 (pl) 2009-12-18 2018-02-28 Novartis Ag Roztwór do przemywania i sposób chromatografii powinowactwa
LT2714714T (lt) 2011-06-01 2018-09-25 Novartis Ag Plovimo tirpalas ir afininės chromatografijos būdas
AU2012290004B2 (en) 2011-08-02 2014-11-27 Takeda Pharmaceutical Company Limited Systems and methods to increase protein yield from recombinant manufacturing processes
WO2022061035A1 (en) 2020-09-16 2022-03-24 Waters Technologies Corporation Improved size exclusion chromatography utilizing low concentration amino acids in size exclusion chromatography mobile phase

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6489292B1 (en) * 1998-11-18 2002-12-03 Novo Nordisk A/S Stable aqueous insulin preparations without phenol and cresol
DE60231016D1 (de) * 2001-06-05 2009-03-12 Genetics Inst Llc Verfahren zur Reinigung stark anionischer Proteine
PT1610820E (pt) * 2003-04-04 2010-12-16 Novartis Ag Formulações de elevada concentração de anticorpos e proteínas
US8084032B2 (en) * 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody

Also Published As

Publication number Publication date
US7501495B2 (en) 2009-03-10
JP4941882B2 (ja) 2012-05-30
EP1698637A1 (de) 2006-09-06
JP2006242957A (ja) 2006-09-14
US20060199948A1 (en) 2006-09-07
EP1698637B1 (de) 2008-10-15

Similar Documents

Publication Publication Date Title
DE602006003108D1 (de) Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
DK1577319T3 (da) Proteinoprensning med præfiltrering
ZA200710330B (en) Methods of purifying FC region containing proteins
ZA200900836B (en) Process for the purification of FC-fusion proteins
DE602005013775D1 (de) Verbesserte methode zur transformation von sojabohnen
IL199753A0 (en) Process for the purification of fc-containing proteins
EA200700094A1 (ru) Способ промышленной очистки образцов клеток для получения терапевтических протеинов
HK1131400A1 (en) Process for the purification of fc-containing proteins
DE602006015297D1 (de) 3-azetidincarbonsäure-derivate zur verwendung als immunsuppressiva
DE602005009729D1 (de) System zur zubereitung von getränken
DE602006009981D1 (de) Methode und Vorrichtung zur Fahrunterstützung
DK2046819T3 (da) Fremgangsmåder til forøgelse af secernering af polypeptider med biologisk aktivitet
DK1941026T3 (da) Fremgangsmåder til fremstilling af protein under anvendelse af anti-senescensforbindelser
DK2061897T3 (da) Fed-batch-fermenteringsproces med høj celledensitet til fremstilling af rekombinant protein
ATE493986T1 (de) Diarylharnstoffe zur behandlung von pulmonaler hypertonie
ATE442898T1 (de) Filtrationsverfahren
DK1817342T3 (da) Fremgangsmåde til affinitetsrensning
ATE552335T1 (de) An das wachstum in suspension angepasste vero- zellinie, verfahren zur gewinnung davon und virale impfstoffe
DK1989189T3 (da) Adsorbenter til proteinoprensning
EP1869230A4 (de) Verfahren zur reinigung von proteinen
DE602004005438D1 (de) Methode zur überwachung des nervensystems
ATE490957T1 (de) Tetrahydrochinoline zur verwendung als modulatoren des mitotischen motor- protein eg5
ZA200900837B (en) Process for the purification of FC-containing proteins
DE602004011674D1 (de) Promotormoleküle zur verwendung in pflanzen
DE602004023969D1 (de) Methode zur herstellung von quervernetzten protein kristallen

Legal Events

Date Code Title Description
8364 No opposition during term of opposition