ATE333469T1 - Verfahren zur reinigung hochanionischer proteine - Google Patents

Verfahren zur reinigung hochanionischer proteine

Info

Publication number
ATE333469T1
ATE333469T1 AT02749564T AT02749564T ATE333469T1 AT E333469 T1 ATE333469 T1 AT E333469T1 AT 02749564 T AT02749564 T AT 02749564T AT 02749564 T AT02749564 T AT 02749564T AT E333469 T1 ATE333469 T1 AT E333469T1
Authority
AT
Austria
Prior art keywords
chanionic
proteins
containing molecules
mixture
purifying highly
Prior art date
Application number
AT02749564T
Other languages
English (en)
Inventor
Jonathan L Coffman
William B Foster
Shujun Sun
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of ATE333469T1 publication Critical patent/ATE333469T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/828—Protein A

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT02749564T 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine ATE333469T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29640201P 2001-06-05 2001-06-05

Publications (1)

Publication Number Publication Date
ATE333469T1 true ATE333469T1 (de) 2006-08-15

Family

ID=23141860

Family Applications (2)

Application Number Title Priority Date Filing Date
AT06010184T ATE421536T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung stark anionischer proteine
AT02749564T ATE333469T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT06010184T ATE421536T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung stark anionischer proteine

Country Status (11)

Country Link
US (1) US7223848B2 (de)
EP (2) EP1404428B1 (de)
JP (1) JP4288153B2 (de)
AT (2) ATE421536T1 (de)
AU (1) AU2008203302B2 (de)
CA (1) CA2448788C (de)
DE (2) DE60213248T2 (de)
DK (2) DK1714982T3 (de)
ES (2) ES2266535T3 (de)
NZ (1) NZ530025A (de)
WO (1) WO2002098531A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084032B2 (en) * 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
DE602006003108D1 (de) * 2005-03-03 2008-11-27 Ajinomoto Kk Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
AU2006329963B2 (en) * 2005-12-06 2011-06-09 Amgen Inc. Polishing steps used in multi-step protein purification processes
AU2007204987B2 (en) * 2006-01-06 2011-11-24 Amgen Inc. Methods and systems for isolating target molecules from complex solutions by column-chromatography using eluants containing organic solvents
CL2007002615A1 (es) * 2006-09-08 2008-04-18 Wyeth Corp Metodos para aislar o purificar un producto que comprende poner el producto enlazado en contacto con al menos una solucion de lavado que comprende arginina y luego eluir el producto; y dicho producto.
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
BRPI0716762A2 (pt) 2006-09-13 2013-09-24 Abbott Lab melhorias da cultura celular
EP2089429B1 (de) * 2006-10-19 2014-11-26 Janssen Biotech, Inc. Verfahren zur herstellung nicht aggregierter antikörper-fc-domänen
TWI610936B (zh) * 2008-10-20 2018-01-11 艾伯維有限公司 使用蛋白質ａ親和性層析進行抗體之分離及純化
TW201028433A (en) 2008-10-20 2010-08-01 Abbott Lab Viral inactivation during purification of antibodies
CN102272145B (zh) * 2009-01-12 2014-07-30 通用电气健康护理生物科学股份公司 亲和色谱基质
WO2011064910A1 (ja) * 2009-11-25 2011-06-03 パナソニック株式会社 免疫測定方法
CA2784278C (en) 2009-12-18 2019-02-26 Novartis Ag Wash solution and method for affinity chromatography
JP6366502B2 (ja) 2011-06-01 2018-08-01 ノバルティス アーゲー アフィニティクロマトグラフィーのための、洗浄溶液および方法
KR20220130692A (ko) * 2020-01-20 2022-09-27 우시 바이올로직스 아일랜드 리미티드 친화성 크로마토그래피를 위한 신규한 세척 완충제 용액

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5115101A (en) 1988-06-08 1992-05-19 Miles Inc. Removal of protein A from antibody preparations
US4983722A (en) * 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5464778A (en) * 1989-03-08 1995-11-07 Board Of Regents Of The University Of Oklahoma Glycoprotein ligand for P-selectin and methods of use thereof
GB9022547D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Purified immunoglobulin
US5726293A (en) * 1992-10-02 1998-03-10 The General Hospital Corporation Affinity purification methods involving imidazole elution
US6277975B1 (en) 1992-10-23 2001-08-21 Genetics Institute, Inc. Fusions of P-selectin ligand protein and polynucleotides encoding same
US5843707A (en) * 1992-10-23 1998-12-01 Genetics Institute, Inc. Nucleic acid encoding a novel P-selectin ligand protein
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
DE69522492T2 (de) * 1994-04-09 2002-05-23 F. Hoffmann-La Roche Ag, Basel Verfahren zur Herstellung von Alpha-Interferon
US6008328A (en) * 1994-10-13 1999-12-28 Amgen Inc. Method for purifying keratinocyte growth factors
SE503424C2 (sv) * 1994-11-14 1996-06-10 Pharmacia Ab Process för rening av rekombinant koagulationsfaktor VIII
FR2737730B1 (fr) * 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
FR2738009B1 (fr) * 1995-08-24 1997-10-31 Centre Nat Rech Scient Procede d'obtention de polysaccharides sulfates
CA2268747A1 (en) * 1996-11-15 1998-05-22 Genentech, Inc. Purification of neurotrophins
DE69739673D1 (de) * 1996-11-27 2009-12-31 Genentech Inc Affinitätsreinigung von Polypeptid-Proteinen auf einer Protein A Matrix
ES2262340T3 (es) * 1998-09-24 2006-11-16 Pharming Intellectual Property Bv Purificacion del fibrinogeno de la leche mediante el uso de cromatografia de intercambio cationico.
AU774419C (en) 1998-10-30 2005-03-03 Genetics Institute, Llc Inhibition of differentiation of cytotoxic T-cells by P-selectin ligand (PSGL) antagonists
EP1147413B1 (de) * 1999-01-21 2006-04-05 Metamorphix, Inc. Inhibitoren für wachstumdifferenzierungsfaktor und ihre anwendungen
CA2404155C (en) * 2000-03-27 2011-08-09 Genetics Institute, Llc. Methods for purifying highly anionic proteins

Also Published As

Publication number Publication date
CA2448788C (en) 2010-08-24
AU2008203302A1 (en) 2008-08-14
DK1404428T3 (da) 2006-10-30
DE60231016D1 (de) 2009-03-12
WO2002098531A1 (en) 2002-12-12
EP1714982A2 (de) 2006-10-25
AU2008203302B2 (en) 2011-10-27
ES2321539T3 (es) 2009-06-08
CA2448788A1 (en) 2002-12-12
DE60213248T2 (de) 2006-11-30
JP2004537042A (ja) 2004-12-09
EP1714982B1 (de) 2009-01-21
ATE421536T1 (de) 2009-02-15
EP1404428A1 (de) 2004-04-07
JP4288153B2 (ja) 2009-07-01
EP1404428B1 (de) 2006-07-19
ES2266535T3 (es) 2007-03-01
US7223848B2 (en) 2007-05-29
NZ530025A (en) 2005-06-24
EP1714982A3 (de) 2006-12-20
EP1404428A4 (de) 2004-12-08
DK1714982T3 (da) 2009-05-04
US20030050450A1 (en) 2003-03-13
DE60213248D1 (de) 2006-08-31

Similar Documents

Publication Publication Date Title
ATE333469T1 (de) Verfahren zur reinigung hochanionischer proteine
DE60030323D1 (de) Verfahren und verbindungen zur verlängerung der halbwertzeiten bei der ausscheidung von biowirksamen verbindungen
ATE278958T1 (de) Reinigung des gewebe plasminogenaktivators (tpa)
ATE278785T1 (de) Neues verfahren zur gewinnung und reinigung von fusionsproteinen
ATE342353T1 (de) Abtrennung von plasmin(ogen) aus proteinlösungen
WO2002057290A3 (en) Novel fibronectin epitopes and proteinaceous molecules capable of binding said epitopes
DE60324912D1 (de) Verfahren zur absorption von sauren gasen
PL374311A1 (en) Methods for purifying protein
DE60320362D1 (de) Verfahren zur Herstellung von Dipeptiden
ATE449104T1 (de) Verfahren zur reinigung von fibrinogen
DE50012831D1 (de) Verfahren zur verbindung von molekularen substanzen
DE60104833D1 (de) Verfahren zur abtrennung von einer basischen aminosäure aus fermentationsbrühen
ATE428728T1 (de) Verfahren zur reinigung von erythropoietin
ATE417056T1 (de) Verfahren zur proteinreinigung
JPH06508058A (ja) 吸着マトリックス
ATE242782T1 (de) Verfahren zur herstellung von peptiden aus biologischen flüssigkeiten und nach diesem verfahren herstellbare peptide
ATE213227T1 (de) Verfahren und vorrichtung zur aufreinigung und anreicherung von molekülen
ATE278699T1 (de) Verfahren zur aufreinigung von nukleinsäuren sowie anionenaustauscher zur durchführung dieses verfahrens
DE60039533D1 (de) Verbessertes verfahren zur herstellung von 2s,3s-n-isobutyl-n-(2-hydroxy-3-amino-4-phenylbutyl)-p-nitrobenzenesulfonylamid hydrochlorid und anderen derivaten von 2-hydroxy-1,3-diamin
ATE349525T1 (de) Histamine und serotonine bindende moleküle
WO2003060067A3 (en) P85-alpha nucleic acids, polypeptides and related methods
DE69432376D1 (de) Verfahren zur Abscheidung von Kohlendioxid aus Verbrennungsabgasen
Slattery et al. Analysis of complex protein mixtures on the HP-G1000A sequencer
DE60100871D1 (de) Verfahren zur Herstellung von Alkylendiamin-N,N'-Dibernsteinsäure-Eisen-Base Komplex mit pH-Kontrolle
WO2005012349A3 (en) Chromatographic process for the purification of notch ligands

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties