PT3272764T - Método para cromatografia de afinidade - Google Patents
Método para cromatografia de afinidadeInfo
- Publication number
- PT3272764T PT3272764T PT171886575T PT17188657T PT3272764T PT 3272764 T PT3272764 T PT 3272764T PT 171886575 T PT171886575 T PT 171886575T PT 17188657 T PT17188657 T PT 17188657T PT 3272764 T PT3272764 T PT 3272764T
- Authority
- PT
- Portugal
- Prior art keywords
- affinity chromatography
- affinity
- chromatography
- Prior art date
Links
- 238000001042 affinity chromatography Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28805909P | 2009-12-18 | 2009-12-18 |
Publications (1)
Publication Number | Publication Date |
---|---|
PT3272764T true PT3272764T (pt) | 2024-04-24 |
Family
ID=43598315
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PT107904526T PT2513134T (pt) | 2009-12-18 | 2010-12-17 | Solução de lavagem e método para cromatografia de afinidade |
PT171886575T PT3272764T (pt) | 2009-12-18 | 2010-12-17 | Método para cromatografia de afinidade |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PT107904526T PT2513134T (pt) | 2009-12-18 | 2010-12-17 | Solução de lavagem e método para cromatografia de afinidade |
Country Status (18)
Country | Link |
---|---|
US (3) | US9505803B2 (pt) |
EP (2) | EP2513134B1 (pt) |
JP (2) | JP5909450B2 (pt) |
KR (1) | KR101858266B1 (pt) |
CN (1) | CN102725304B (pt) |
AU (1) | AU2010332778B2 (pt) |
BR (1) | BR112012014937B1 (pt) |
CA (1) | CA2784278C (pt) |
DK (2) | DK2513134T3 (pt) |
ES (1) | ES2651065T3 (pt) |
IL (1) | IL220164B (pt) |
LT (2) | LT2513134T (pt) |
MX (1) | MX2012007103A (pt) |
PL (2) | PL2513134T3 (pt) |
PT (2) | PT2513134T (pt) |
RU (1) | RU2564569C2 (pt) |
SG (2) | SG10201408384PA (pt) |
WO (1) | WO2011073389A1 (pt) |
Families Citing this family (33)
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US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
EP2527425A1 (en) | 2006-09-13 | 2012-11-28 | Abbott Laboratories | Cell culture improvements |
AU2009307737B2 (en) | 2008-10-20 | 2015-07-23 | Abbvie Inc. | Viral inactivation during purification of antibodies |
SG10201702922VA (en) * | 2008-10-20 | 2017-06-29 | Abbvie Inc | Isolation and purification of antibodies using protein a affinity chromatography |
DK2513134T3 (en) | 2009-12-18 | 2017-12-18 | Novartis Ag | Washing solution and process for affinity chromatography |
CA2784959C (en) | 2010-01-22 | 2018-06-26 | Boehringer Ingelheim International Gmbh | Chromatographic method for purifying fc-containing proteins |
KR101953405B1 (ko) | 2011-06-01 | 2019-02-28 | 노파르티스 아게 | 친화성 크로마토그래피를 위한 세척 용액 및 방법 |
EP2766397B1 (en) | 2011-10-11 | 2018-05-30 | F.Hoffmann-La Roche Ag | Improved assembly of bispecific antibodies |
CA2910065C (en) * | 2013-05-15 | 2023-09-19 | Medimmune Limited | Purification of recombinantly produced polypeptides |
EP3041593A4 (en) * | 2013-09-05 | 2017-08-30 | F. Hoffmann-La Roche AG | Method for chromatography reuse |
EP2986307A4 (en) | 2014-01-03 | 2017-04-12 | Bio-rad Laboratories, Inc. | Removal of impurities from protein a eluates |
US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
MX2017011121A (es) | 2015-03-13 | 2017-11-28 | Bristol Myers Squibb Co | Uso de lavados alcalinos durante la cromatografia para remover impurezas. |
EP3337812B1 (en) | 2015-08-21 | 2021-04-28 | F. Hoffmann-La Roche AG | Method for the reduction of host cell proteins in affinity chromatography |
US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
WO2017194597A1 (en) | 2016-05-11 | 2017-11-16 | Ge Healthcare Bioprocess R&D Ab | Separation matrix |
US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
JP7106187B2 (ja) | 2016-05-11 | 2022-07-26 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックスを保存する方法 |
US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
US11753438B2 (en) | 2016-05-11 | 2023-09-12 | Cytiva Bioprocess R&D Ab | Method of cleaning and/or sanitizing a separation matrix |
US10688412B2 (en) * | 2016-07-25 | 2020-06-23 | Cehpalon, Inc. | Affinity chromatography wash buffer |
WO2019036626A1 (en) | 2017-08-17 | 2019-02-21 | Just Biotherapeutics, Inc. | PROCESS FOR PURIFYING GLYCOSYLATED PROTEIN FROM GALECTINES AND OTHER HOST CELL CONTAMINANTS |
CN107793469B (zh) * | 2017-10-16 | 2021-07-30 | 上海药明生物技术有限公司 | 一种去除宿主细胞蛋白含量的亲和纯化工艺 |
IL311002A (en) * | 2017-12-21 | 2024-04-01 | Genzyme Corp | Methods for improved removal of impurities during protein A chromatography |
WO2019183334A1 (en) | 2018-03-21 | 2019-09-26 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbents, devices and methods |
SG11202009629YA (en) | 2018-04-02 | 2020-10-29 | Amgen Inc | Erenumab compositions and uses thereof |
CN112105927B (zh) * | 2018-05-08 | 2024-06-25 | 沃特世科技公司 | 可用于pH梯度阳离子交换色谱法的方法、组合物和试剂盒 |
WO2021072005A1 (en) * | 2019-10-08 | 2021-04-15 | North Carolina State University | Immunoglobulin purification peptides and their use |
AU2021244846A1 (en) * | 2020-03-27 | 2022-11-24 | HaemaLogiX Ltd | Composition and method |
US20230143163A1 (en) * | 2020-04-27 | 2023-05-11 | Alexion Pharmaceuticals, Inc. | Protein a chromatography purification of an antibody |
CN112827217A (zh) * | 2020-12-29 | 2021-05-25 | 苏州良辰生物医药科技有限公司 | 一种层析柱清洗液及其应用 |
WO2024126337A1 (en) * | 2022-12-12 | 2024-06-20 | H. Lundbeck A/S | Arginine wash in protein purification |
Family Cites Families (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4704366A (en) | 1984-06-22 | 1987-11-03 | Bio-Rad Laboratories, Inc. | Process for binding IgG to protein A |
US4857320A (en) * | 1987-02-19 | 1989-08-15 | Monsanto Company | Method of enhancing the solubility of tissue plasminogen activator |
JPH01238534A (ja) * | 1988-03-17 | 1989-09-22 | Mitsui Toatsu Chem Inc | エンドトキシンの除去方法 |
US5429746A (en) | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
ATE371673T1 (de) | 1996-11-27 | 2007-09-15 | Genentech Inc | Affinitätsreinigung von polypeptiden an einer protein a-matrix |
ES2198913T5 (es) | 1998-05-06 | 2013-11-18 | Genentech, Inc. | Purificación de proteínas mediante cromatografía de intercambio iónico |
IL139609A (en) | 1998-06-01 | 2006-06-11 | Genentech Inc | Separation of polypeptides monomers |
JP4391086B2 (ja) | 2001-03-09 | 2009-12-24 | 中外製薬株式会社 | タンパク質精製方法 |
DK1404428T3 (da) | 2001-06-05 | 2006-10-30 | Genetics Inst Llc | Fremgangsmåder til oprensning af stærkt anioniske proteiner |
AU2003210802B2 (en) | 2002-02-05 | 2009-09-10 | Genentech Inc. | Protein purification |
AU2003262087B2 (en) | 2002-09-11 | 2010-11-11 | Chugai Seiyaku Kabushiki Kaisha | Protein purification method |
KR20050083827A (ko) * | 2002-10-24 | 2005-08-26 | 이노스 파마슈티칼스, 인코포레이티드 | 서방성 l-아르기닌 포뮬레이션 및 이의 제조방법 및 용도 |
KR20050089151A (ko) * | 2002-11-26 | 2005-09-07 | 프로테인 디자인 랩스 인코포레이티드 | 혈관형성을 조절하는 α5β1 인테그린에 대한 키메라성 및인간화 항체 |
EP2174663A1 (en) * | 2003-01-08 | 2010-04-14 | Novartis Vaccines and Diagnostics, Inc. | Stabilized aqueous compositions comprising tissue factor pathway inhibitor (TFPI) or tissue factor pathway inhibitor variant |
GB0304576D0 (en) | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
EP1601697B1 (en) | 2003-02-28 | 2007-05-30 | Lonza Biologics plc | Antibody purification by Protein A and ion exchange chromatography |
CN102276717B (zh) | 2003-10-27 | 2015-09-09 | 惠氏公司 | 使用羟磷灰石层析除去高分子量聚集体 |
US8084032B2 (en) | 2004-01-21 | 2011-12-27 | Ajinomoto Co., Inc. | Purification method which prevents denaturation of an antibody |
EP1698637B1 (en) | 2005-03-03 | 2008-10-15 | Ajinomoto Co., Inc. | A method for improving recovery yield in protein purification with gel filtration chromatography using arginine |
WO2006099308A2 (en) * | 2005-03-11 | 2006-09-21 | Wyeth | A method of weak partitioning chromatography |
CN101365948B (zh) | 2006-01-06 | 2012-11-07 | Emd密理博公司 | 亲和层析基质及其制备和使用方法 |
US8263750B2 (en) | 2006-03-16 | 2012-09-11 | Amgen Inc. | Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step |
CA2645739A1 (en) | 2006-03-20 | 2007-09-27 | Medarex, Inc. | Protein purification |
JP2009539767A (ja) * | 2006-06-02 | 2009-11-19 | ワイス | 精製および治療方法のためのTGF−βスーパーファミリータンパク質およびペプチドの使用 |
US7754689B2 (en) * | 2006-06-02 | 2010-07-13 | Wyeth Llc | Finger-1 peptide analogs of the TGF-β superfamily |
EA017152B1 (ru) | 2006-08-28 | 2012-10-30 | Арес Трейдинг С.А. | СПОСОБ ОЧИСТКИ Fc-СОДЕРЖАЩИХ БЕЛКОВ |
MX2009002576A (es) * | 2006-09-08 | 2009-03-20 | Wyeth Corp | Lavado de arginina en purificacion de proteina utilizando cromatografia de afinidad. |
EP2114984A2 (en) | 2007-01-17 | 2009-11-11 | Merck Serono S.A. | Process for the purification of fc-containing proteins |
CA2687837A1 (en) | 2007-06-01 | 2008-12-04 | F. Hoffmann-La Roche Ag | Immunoglobulin purification |
CN103396480A (zh) | 2008-05-15 | 2013-11-20 | 诺沃—诺迪斯克有限公司 | 抗体纯化方法 |
AU2009282234A1 (en) | 2008-08-14 | 2010-02-18 | Merck Sharp & Dohme Corp. | Methods for purifying antibodies using protein a affinity chromatography |
DK2513134T3 (en) | 2009-12-18 | 2017-12-18 | Novartis Ag | Washing solution and process for affinity chromatography |
KR101953405B1 (ko) * | 2011-06-01 | 2019-02-28 | 노파르티스 아게 | 친화성 크로마토그래피를 위한 세척 용액 및 방법 |
-
2010
- 2010-12-17 DK DK10790452.6T patent/DK2513134T3/en active
- 2010-12-17 PL PL10790452T patent/PL2513134T3/pl unknown
- 2010-12-17 SG SG10201408384PA patent/SG10201408384PA/en unknown
- 2010-12-17 JP JP2012543787A patent/JP5909450B2/ja active Active
- 2010-12-17 PT PT107904526T patent/PT2513134T/pt unknown
- 2010-12-17 RU RU2012130151/10A patent/RU2564569C2/ru active
- 2010-12-17 AU AU2010332778A patent/AU2010332778B2/en active Active
- 2010-12-17 SG SG2012044707A patent/SG181771A1/en unknown
- 2010-12-17 WO PCT/EP2010/070076 patent/WO2011073389A1/en active Application Filing
- 2010-12-17 ES ES10790452.6T patent/ES2651065T3/es active Active
- 2010-12-17 PT PT171886575T patent/PT3272764T/pt unknown
- 2010-12-17 PL PL17188657.5T patent/PL3272764T3/pl unknown
- 2010-12-17 LT LTEP10790452.6T patent/LT2513134T/lt unknown
- 2010-12-17 EP EP10790452.6A patent/EP2513134B1/en active Active
- 2010-12-17 DK DK17188657.5T patent/DK3272764T3/da active
- 2010-12-17 LT LTEP17188657.5T patent/LT3272764T/lt unknown
- 2010-12-17 CA CA2784278A patent/CA2784278C/en active Active
- 2010-12-17 KR KR1020127015524A patent/KR101858266B1/ko active IP Right Grant
- 2010-12-17 EP EP17188657.5A patent/EP3272764B1/en active Active
- 2010-12-17 BR BR112012014937-7A patent/BR112012014937B1/pt active IP Right Grant
- 2010-12-17 CN CN201080057457.6A patent/CN102725304B/zh active Active
- 2010-12-17 US US13/516,960 patent/US9505803B2/en active Active
- 2010-12-17 MX MX2012007103A patent/MX2012007103A/es unknown
-
2012
- 2012-06-04 IL IL220164A patent/IL220164B/en active IP Right Grant
-
2016
- 2016-02-08 JP JP2016021798A patent/JP2016128495A/ja active Pending
- 2016-10-28 US US15/336,878 patent/US20170044211A1/en not_active Abandoned
-
2019
- 2019-03-25 US US16/363,170 patent/US20190233469A1/en not_active Abandoned
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