KR101454316B1 - 활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법 - Google Patents

활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법 Download PDF

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KR101454316B1
KR101454316B1 KR1020120090341A KR20120090341A KR101454316B1 KR 101454316 B1 KR101454316 B1 KR 101454316B1 KR 1020120090341 A KR1020120090341 A KR 1020120090341A KR 20120090341 A KR20120090341 A KR 20120090341A KR 101454316 B1 KR101454316 B1 KR 101454316B1
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South Korea
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tnfr
fusion protein
buffer
protein
column
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English (en)
Korean (ko)
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KR20130020644A (ko
Inventor
원혜순
성병제
안용호
박상경
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한화케미칼 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020120090341A 2011-08-17 2012-08-17 활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법 Expired - Fee Related KR101454316B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20110081854 2011-08-17
KR1020110081854 2011-08-17

Publications (2)

Publication Number Publication Date
KR20130020644A KR20130020644A (ko) 2013-02-27
KR101454316B1 true KR101454316B1 (ko) 2014-10-27

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ID=47715279

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KR1020120090341A Expired - Fee Related KR101454316B1 (ko) 2011-08-17 2012-08-17 활성형 ＴＮＦＲ-Ｆｃ 융합 단백질을 제조하는 방법

Country Status (6)

Country Link
US (1) US9279014B2 (enExample)
EP (1) EP2753634B1 (enExample)
JP (2) JP6223338B2 (enExample)
KR (1) KR101454316B1 (enExample)
ES (1) ES2651483T3 (enExample)
WO (1) WO2013025079A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014009031A2 (pt) 2011-10-18 2017-05-09 Coherus Biosciences Inc formulações de etanercept estabilizadas com íons de metais
KR101494072B1 (ko) * 2012-03-12 2015-02-17 한화케미칼 주식회사 변이된 글루타민 합성효소 유전자를 포함하는 벡터 및 이를 이용한 목적 단백질의 생산방법
LT2895188T (lt) 2012-09-11 2018-04-10 Coherus Biosciences, Inc. Aukšto grynumo ir geros išeigos teisingos struktūros etanerceptas
BR112015019341A2 (pt) 2013-02-13 2017-08-22 Lab Francais Du Fractionnement Anticorpo anti-tnf-alfa, composição que compreende o anticorpo, método para produzir uma população de anticorpos, células epiteliais da glândula mamária, mamífero não humano transgênico, e, composição de anticorpo anti-tnf monoclonal
BR112016029157A8 (pt) * 2014-06-13 2021-07-06 Lupin Ltd processo para purificar a proteína de fusão tnfr:fc
ES2733298T3 (es) 2014-07-18 2019-11-28 Sandoz Ag Cuantificación de TNFR2:Fc plegado erróneamente
KR102018608B1 (ko) * 2014-12-31 2019-09-06 주식회사 엘지화학 목적하는 함량으로 불순물을 포함하는 TNFR-Fc 융합 단백질의 제조방법
WO2016149139A1 (en) * 2015-03-13 2016-09-22 Samsung Bioepis Co., Ltd. Anti-tnf-alpha polypeptide composition and use thereof
US11952399B2 (en) * 2018-12-18 2024-04-09 Amgen Inc. Methods for purifying proteins
US12202857B2 (en) 2020-05-08 2025-01-21 Waters Technologies Corporation Methods for heat-assisted enzyme digestion
US12411064B2 (en) 2020-05-08 2025-09-09 Waters Technologies Corporation Methods for heat-assisted enzyme digestion

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TR200504220T2 (tr) 1998-12-17 2007-04-24 Biogen Idec Ma Inc. Aktif limfotoksin-beta reseptör imunoglobülin şimeAktif limfotoksin-beta reseptör imunoglobülin şimerik proteinlerinin yüksek düzey ifadesi ve saflaştrik proteinlerinin yüksek düzey ifadesi ve saflaştırılması için bir yöntem.ırılması için bir yöntem.
US7294481B1 (en) 1999-01-05 2007-11-13 Immunex Corporation Method for producing recombinant proteins
US20030180287A1 (en) 2002-02-27 2003-09-25 Immunex Corporation Polypeptide formulation
ATE462716T1 (de) * 2003-10-24 2010-04-15 Amgen Inc Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen
CA2554929A1 (en) * 2004-01-30 2005-08-18 Amgen Inc. Process for purifying proteins
BRPI0908270A2 (pt) * 2008-02-29 2019-09-10 Biogen Idec Inc proteínas de fusão de imunoglobulinas purificadas e método para a sua purificação
CN102675465A (zh) * 2011-03-09 2012-09-19 上海赛金生物医药有限公司 重组人ⅱ型肿瘤坏死因子受体-抗体融合蛋白的纯化方法
WO2012176158A1 (en) * 2011-06-24 2012-12-27 Dr. Reddy's Laboratories Limited Purification of chimeric protein

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2008) VOL.105, NO.5, pp.454-459 *
JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2008) VOL.105, NO.5, pp.454-459*

Also Published As

Publication number Publication date
ES2651483T3 (es) 2018-01-26
JP6223338B2 (ja) 2017-11-01
EP2753634A4 (en) 2015-05-20
WO2013025079A1 (en) 2013-02-21
US20140316114A1 (en) 2014-10-23
JP2014521364A (ja) 2014-08-28
EP2753634A1 (en) 2014-07-16
KR20130020644A (ko) 2013-02-27
EP2753634B1 (en) 2017-11-01
JP2016168049A (ja) 2016-09-23
US9279014B2 (en) 2016-03-08

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