JP2016529489A5 - - Google Patents
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- Publication number
- JP2016529489A5 JP2016529489A5 JP2016525805A JP2016525805A JP2016529489A5 JP 2016529489 A5 JP2016529489 A5 JP 2016529489A5 JP 2016525805 A JP2016525805 A JP 2016525805A JP 2016525805 A JP2016525805 A JP 2016525805A JP 2016529489 A5 JP2016529489 A5 JP 2016529489A5
- Authority
- JP
- Japan
- Prior art keywords
- polypeptide
- buffer
- contaminants
- gradient
- chromatography material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 59
- 229920001184 polypeptide Polymers 0.000 claims 59
- 102000004196 processed proteins & peptides Human genes 0.000 claims 59
- 108090000765 processed proteins & peptides Proteins 0.000 claims 59
- 239000000463 material Substances 0.000 claims 26
- 239000000203 mixture Substances 0.000 claims 26
- 239000000872 buffer Substances 0.000 claims 23
- 239000000356 contaminant Substances 0.000 claims 23
- 238000004587 chromatography analysis Methods 0.000 claims 16
- 238000004255 ion exchange chromatography Methods 0.000 claims 13
- 150000003839 salts Chemical class 0.000 claims 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 8
- 239000012149 elution buffer Substances 0.000 claims 8
- 239000012160 loading buffer Substances 0.000 claims 8
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims 4
- 238000000926 separation method Methods 0.000 claims 4
- 239000011780 sodium chloride Substances 0.000 claims 4
- 239000007995 HEPES buffer Substances 0.000 claims 3
- 239000002253 acid Substances 0.000 claims 3
- 150000001413 amino acids Chemical class 0.000 claims 3
- 238000005277 cation exchange chromatography Methods 0.000 claims 3
- 238000010494 dissociation reaction Methods 0.000 claims 3
- 230000005593 dissociations Effects 0.000 claims 3
- 238000005342 ion exchange Methods 0.000 claims 3
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical group NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 claims 2
- 150000001412 amines Chemical group 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 230000007935 neutral effect Effects 0.000 claims 2
- 150000003512 tertiary amines Chemical class 0.000 claims 2
- -1 (NH 4 ) 2 SO 4 Substances 0.000 claims 1
- 239000007991 ACES buffer Substances 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 238000005349 anion exchange Methods 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 230000003139 buffering effect Effects 0.000 claims 1
- 238000005341 cation exchange Methods 0.000 claims 1
- 238000013375 chromatographic separation Methods 0.000 claims 1
- 239000007857 degradation product Substances 0.000 claims 1
- 238000012434 mixed-mode chromatography Methods 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361845890P | 2013-07-12 | 2013-07-12 | |
| US61/845,890 | 2013-07-12 | ||
| PCT/US2014/046338 WO2015006686A1 (en) | 2013-07-12 | 2014-07-11 | Elucidation of ion exchange chromatography input optimization |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018242206A Division JP2019090818A (ja) | 2013-07-12 | 2018-12-26 | イオン交換クロマトグラフィーのインプットの最適化の解明 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016529489A JP2016529489A (ja) | 2016-09-23 |
| JP2016529489A5 true JP2016529489A5 (enExample) | 2017-08-31 |
| JP6462681B2 JP6462681B2 (ja) | 2019-01-30 |
Family
ID=51257626
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016525805A Active JP6462681B2 (ja) | 2013-07-12 | 2014-07-11 | イオン交換クロマトグラフィーのインプットの最適化の解明 |
| JP2018242206A Pending JP2019090818A (ja) | 2013-07-12 | 2018-12-26 | イオン交換クロマトグラフィーのインプットの最適化の解明 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018242206A Pending JP2019090818A (ja) | 2013-07-12 | 2018-12-26 | イオン交換クロマトグラフィーのインプットの最適化の解明 |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US10274466B2 (enExample) |
| EP (2) | EP3536699A1 (enExample) |
| JP (2) | JP6462681B2 (enExample) |
| KR (1) | KR102251127B1 (enExample) |
| CN (1) | CN105377873B (enExample) |
| AU (1) | AU2014287044B2 (enExample) |
| BR (1) | BR112016000231A8 (enExample) |
| CA (1) | CA2918052A1 (enExample) |
| ES (1) | ES2705700T3 (enExample) |
| HR (1) | HRP20190071T1 (enExample) |
| IL (1) | IL243305B (enExample) |
| MX (1) | MX368107B (enExample) |
| PL (1) | PL3019516T3 (enExample) |
| RU (1) | RU2016104542A (enExample) |
| SG (1) | SG11201600221YA (enExample) |
| SI (1) | SI3019516T1 (enExample) |
| TR (1) | TR201818966T4 (enExample) |
| WO (1) | WO2015006686A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2705700T3 (es) | 2013-07-12 | 2019-03-26 | Hoffmann La Roche | Elucidación de la optimización de entrada en cromatografía de intercambio iónico |
| CN107110835B (zh) | 2014-11-12 | 2019-07-26 | 通用电气健康护理生物科学股份公司 | 用于确定实验参数对液相色谱协议的影响的方法和系统 |
| GB2539420B (en) * | 2015-06-16 | 2021-01-13 | Cytiva Sweden Ab | Determination of chromatography conditions |
| BR112020019882A2 (pt) * | 2018-04-02 | 2021-01-05 | Amgen Inc. | Composições de erenumabe e seus usos |
| JP2021530247A (ja) * | 2018-07-05 | 2021-11-11 | オベーション ダイアグノスティクス エルエルシー | 癌の診断のためのＢＣｌ−２抗体とイムノアッセイ |
| KR20200080748A (ko) * | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법 |
| WO2024261724A1 (en) * | 2023-06-23 | 2024-12-26 | Lupin Limited | Ion-exchange chromatography for separation and analysis of charge variants |
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| FR2413974A1 (fr) | 1978-01-06 | 1979-08-03 | David Bernard | Sechoir pour feuilles imprimees par serigraphie |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
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| IL85035A0 (en) | 1987-01-08 | 1988-06-30 | Int Genetic Eng | Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same |
| ES2037273T3 (es) | 1987-04-11 | 1993-06-16 | Ciba-Geigy Ag | Proceso de focalizacion isoelectrica y medios para llevar a cabo dicho proceso. |
| DE3850542T2 (de) | 1987-09-23 | 1994-11-24 | Bristol Myers Squibb Co | Antikörper-Heterokonjugate zur Töting von HIV-infizierten Zellen. |
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| US5175384A (en) | 1988-12-05 | 1992-12-29 | Genpharm International | Transgenic mice depleted in mature t-cells and methods for making transgenic mice |
| DE3920358A1 (de) | 1989-06-22 | 1991-01-17 | Behringwerke Ag | Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung |
| ATE144793T1 (de) | 1989-06-29 | 1996-11-15 | Medarex Inc | Bispezifische reagenzien für die aids-therapie |
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| US5571894A (en) | 1991-02-05 | 1996-11-05 | Ciba-Geigy Corporation | Recombinant antibodies specific for a growth factor receptor |
| WO1992020373A1 (en) | 1991-05-14 | 1992-11-26 | Repligen Corporation | Heteroconjugate antibodies for treatment of hiv infection |
| EP0590058B1 (en) | 1991-06-14 | 2003-11-26 | Genentech, Inc. | HUMANIZED Heregulin ANTIBODy |
| MX9204374A (es) | 1991-07-25 | 1993-03-01 | Idec Pharma Corp | Anticuerpo recombinante y metodo para su produccion. |
| WO1993006217A1 (en) | 1991-09-19 | 1993-04-01 | Genentech, Inc. | EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES |
| US5565332A (en) | 1991-09-23 | 1996-10-15 | Medical Research Council | Production of chimeric antibodies - a combinatorial approach |
| FI941572L (fi) | 1991-10-07 | 1994-05-27 | Oncologix Inc | Anti-erbB-2-monoklonaalisten vasta-aineiden yhdistelmä ja käyttömenetelmä |
| WO1993008829A1 (en) | 1991-11-04 | 1993-05-13 | The Regents Of The University Of California | Compositions that mediate killing of hiv-infected cells |
| AU3178993A (en) | 1991-11-25 | 1993-06-28 | Enzon, Inc. | Multivalent antigen-binding proteins |
| ATE419355T1 (de) | 1992-02-06 | 2009-01-15 | Novartis Vaccines & Diagnostic | Marker für krebs und biosynthetisches bindeprotein dafür |
| EP0626012B1 (en) | 1992-02-11 | 2003-07-09 | Cell Genesys, Inc. | Homogenotization of gene-targeting events |
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| ES2091684T3 (es) | 1992-11-13 | 1996-11-01 | Idec Pharma Corp | Aplicacion terapeutica de anticuerpos quimericos y radiomarcados contra el antigeno de diferenciacion restringida de los linfocitos b humanos para el tratamiento del linfoma de las celulas b. |
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| WO2014078729A1 (en) | 2012-11-15 | 2014-05-22 | Genentech, Inc. | IONIC STRENGTH-MEDIATED pH GRADIENT ION EXCHANGE CHROMATOGRAPHY |
| ES2705700T3 (es) | 2013-07-12 | 2019-03-26 | Hoffmann La Roche | Elucidación de la optimización de entrada en cromatografía de intercambio iónico |
-
2014
- 2014-07-11 ES ES14745049T patent/ES2705700T3/es active Active
- 2014-07-11 PL PL14745049T patent/PL3019516T3/pl unknown
- 2014-07-11 CN CN201480039460.3A patent/CN105377873B/zh active Active
- 2014-07-11 AU AU2014287044A patent/AU2014287044B2/en active Active
- 2014-07-11 WO PCT/US2014/046338 patent/WO2015006686A1/en not_active Ceased
- 2014-07-11 BR BR112016000231A patent/BR112016000231A8/pt active Search and Examination
- 2014-07-11 KR KR1020167003178A patent/KR102251127B1/ko active Active
- 2014-07-11 HR HRP20190071TT patent/HRP20190071T1/hr unknown
- 2014-07-11 SG SG11201600221YA patent/SG11201600221YA/en unknown
- 2014-07-11 EP EP18204792.8A patent/EP3536699A1/en not_active Withdrawn
- 2014-07-11 SI SI201431039T patent/SI3019516T1/sl unknown
- 2014-07-11 CA CA2918052A patent/CA2918052A1/en not_active Abandoned
- 2014-07-11 TR TR2018/18966T patent/TR201818966T4/tr unknown
- 2014-07-11 JP JP2016525805A patent/JP6462681B2/ja active Active
- 2014-07-11 EP EP14745049.8A patent/EP3019516B1/en active Active
- 2014-07-11 MX MX2016000181A patent/MX368107B/es active IP Right Grant
- 2014-07-11 RU RU2016104542A patent/RU2016104542A/ru not_active Application Discontinuation
- 2014-07-11 US US14/904,422 patent/US10274466B2/en active Active
-
2015
- 2015-12-23 IL IL243305A patent/IL243305B/en active IP Right Grant
-
2018
- 2018-12-26 JP JP2018242206A patent/JP2019090818A/ja active Pending
-
2019
- 2019-03-12 US US16/351,372 patent/US10921297B2/en active Active
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