JP2018516229A5 - - Google Patents

Download PDF

Info

Publication number
JP2018516229A5
JP2018516229A5 JP2017546779A JP2017546779A JP2018516229A5 JP 2018516229 A5 JP2018516229 A5 JP 2018516229A5 JP 2017546779 A JP2017546779 A JP 2017546779A JP 2017546779 A JP2017546779 A JP 2017546779A JP 2018516229 A5 JP2018516229 A5 JP 2018516229A5
Authority
JP
Japan
Prior art keywords
dsba
polypeptide
antibody
sample
optionally
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2017546779A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018516229A (ja
JP6865689B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2016/021059 external-priority patent/WO2016144824A1/en
Publication of JP2018516229A publication Critical patent/JP2018516229A/ja
Publication of JP2018516229A5 publication Critical patent/JP2018516229A5/ja
Application granted granted Critical
Publication of JP6865689B2 publication Critical patent/JP6865689B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2017546779A 2015-03-06 2016-03-04 超精製DsbA及びDsbC、ならびにそれらの作製及び使用方法 Active JP6865689B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562129701P 2015-03-06 2015-03-06
US62/129,701 2015-03-06
PCT/US2016/021059 WO2016144824A1 (en) 2015-03-06 2016-03-04 Ultrapurified dsba and dsbc and methods of making and using the same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2021001186A Division JP7191132B2 (ja) 2015-03-06 2021-01-07 超精製DsbA及びDsbC、ならびにそれらの作製及び使用方法

Publications (3)

Publication Number Publication Date
JP2018516229A JP2018516229A (ja) 2018-06-21
JP2018516229A5 true JP2018516229A5 (enExample) 2019-04-11
JP6865689B2 JP6865689B2 (ja) 2021-04-28

Family

ID=55637452

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017546779A Active JP6865689B2 (ja) 2015-03-06 2016-03-04 超精製DsbA及びDsbC、ならびにそれらの作製及び使用方法
JP2021001186A Active JP7191132B2 (ja) 2015-03-06 2021-01-07 超精製DsbA及びDsbC、ならびにそれらの作製及び使用方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2021001186A Active JP7191132B2 (ja) 2015-03-06 2021-01-07 超精製DsbA及びDsbC、ならびにそれらの作製及び使用方法

Country Status (17)

Country Link
US (2) US10073098B2 (enExample)
EP (2) EP3636749B1 (enExample)
JP (2) JP6865689B2 (enExample)
KR (1) KR20170122216A (enExample)
CN (1) CN107531749A (enExample)
AR (1) AR103852A1 (enExample)
AU (1) AU2016230018A1 (enExample)
CA (1) CA2978256A1 (enExample)
ES (1) ES2761726T3 (enExample)
HK (1) HK1247936A1 (enExample)
HR (1) HRP20192217T1 (enExample)
IL (1) IL254166A0 (enExample)
MX (2) MX387454B (enExample)
PL (1) PL3265557T3 (enExample)
SG (2) SG11201707186YA (enExample)
SI (1) SI3265557T1 (enExample)
WO (1) WO2016144824A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9725710B2 (en) 2014-01-08 2017-08-08 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
ES2761726T3 (es) 2015-03-06 2020-05-20 Hoffmann La Roche DsbA y DsbC ultrapurificadas y procedimientos de preparación y uso de las mismas
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
JP2021507561A (ja) 2017-12-14 2021-02-22 フロデザイン ソニックス, インク.Flodesign Sonics, Inc. 音響トランスデューサドライバ及びコントローラ
TWI870412B (zh) * 2019-06-05 2025-01-21 美商建南德克公司 過載層析管柱之再生方法
MX2022011780A (es) 2020-04-09 2022-11-10 Cytomx Therapeutics Inc Composiciones que contienen anticuerpos activables.
US12540929B2 (en) * 2021-12-17 2026-02-03 Dionex Corporation Desalting system for chromatography
CN115028691B (zh) * 2022-06-29 2024-09-10 青岛硕景生物科技有限公司 一种检测试纸用的独立质控系统、杂交瘤细胞株、质控线用包被单克隆抗体及其应用
CN119874933A (zh) * 2024-11-20 2025-04-25 金宇保灵生物药品有限公司 一种基于大肠杆菌的纳米抗体融合表达体系及其制备方法

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2046920B1 (enExample) 1969-06-19 1974-05-03 Citizen Watch Co Ltd
US3691016A (en) 1970-04-17 1972-09-12 Monsanto Co Process for the preparation of insoluble enzymes
US3940475A (en) 1970-06-11 1976-02-24 Biological Developments, Inc. Radioimmune method of assaying quantitatively for a hapten
CA1023287A (en) 1972-12-08 1977-12-27 Boehringer Mannheim G.M.B.H. Process for the preparation of carrier-bound proteins
US4195128A (en) 1976-05-03 1980-03-25 Bayer Aktiengesellschaft Polymeric carrier bound ligands
US4330440A (en) 1977-02-08 1982-05-18 Development Finance Corporation Of New Zealand Activated matrix and method of activation
CA1093991A (en) 1977-02-17 1981-01-20 Hideo Hirohara Enzyme immobilization with pullulan gel
US4229537A (en) 1978-02-09 1980-10-21 New York University Preparation of trichloro-s-triazine activated supports for coupling ligands
US4376110A (en) 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
US4676980A (en) 1985-09-23 1987-06-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target specific cross-linked heteroantibodies
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
US5508192A (en) 1990-11-09 1996-04-16 Board Of Regents, The University Of Texas System Bacterial host strains for producing proteolytically sensitive polypeptides
US5264365A (en) 1990-11-09 1993-11-23 Board Of Regents, The University Of Texas System Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides
US5571894A (en) 1991-02-05 1996-11-05 Ciba-Geigy Corporation Recombinant antibodies specific for a growth factor receptor
EP0940468A1 (en) 1991-06-14 1999-09-08 Genentech, Inc. Humanized antibody variable domain
MX9204374A (es) 1991-07-25 1993-03-01 Idec Pharma Corp Anticuerpo recombinante y metodo para su produccion.
EP0604580A1 (en) 1991-09-19 1994-07-06 Genentech, Inc. EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab') 2? ANTIBODIES
FI941572L (fi) 1991-10-07 1994-05-27 Oncologix Inc Anti-erbB-2-monoklonaalisten vasta-aineiden yhdistelmä ja käyttömenetelmä
WO1993008829A1 (en) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions that mediate killing of hiv-infected cells
JPH07501451A (ja) 1991-11-25 1995-02-16 エンゾン・インコーポレイテッド 多価抗原結合タンパク質
DE69333807T2 (de) 1992-02-06 2006-02-02 Chiron Corp., Emeryville Marker für krebs und biosynthetisches bindeprotein dafür
DE69308573T2 (de) 1992-08-17 1997-08-07 Genentech Inc Bispezifische immunoadhesine
GB9325718D0 (en) * 1993-12-16 1994-02-16 Ars Holding 89 Nv Sensor device for sandwich assay
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
US5789199A (en) 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US6027888A (en) 1996-04-05 2000-02-22 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells
US6083715A (en) 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
JP2000083670A (ja) * 1998-09-09 2000-03-28 Hsp Kenkyusho:Kk DsbA/DsbB/DsbC/DsbD発現プラスミド
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
CA2364568A1 (en) * 1999-03-17 2000-09-21 Adrian Woolfson Method for refolding molecules of polypeptides containing ig domains
US6872563B1 (en) * 1999-10-05 2005-03-29 President And Fellows Of Harvard College Compositions and methods for production of disulfide bond containing proteins in host cells
ES2637801T3 (es) 2000-04-11 2017-10-17 Genentech, Inc. Anticuerpos multivalentes y usos de los mismos
MXPA03005273A (es) 2000-12-14 2003-09-25 Genentech Inc Anticuerpos producidos procarioticamente y sus usos.
EP1345967B1 (en) 2000-12-22 2011-08-10 Grad, Carole, Legal representative of Kaplan, Howard Phage display libraries of human v h fragments
JP2005289809A (ja) 2001-10-24 2005-10-20 Vlaams Interuniversitair Inst Voor Biotechnologie Vzw (Vib Vzw) 突然変異重鎖抗体
SI1639011T1 (sl) 2003-06-30 2009-04-30 Domantis Ltd Pegilirana protitelesa z enojno domeno (dAb)
KR100956913B1 (ko) 2003-12-19 2010-05-11 제넨테크, 인크. 치료제로서 유용한 일가 항체 단편
KR101270829B1 (ko) 2004-09-23 2013-06-07 제넨테크, 인크. 시스테인 유전자조작 항체 및 접합체
CN100475965C (zh) 2005-07-22 2009-04-08 上海高科联合生物技术研发有限公司 一种大肠杆菌高效外分泌表达溶葡萄球菌酶的方法
EP2035456A1 (en) 2006-06-22 2009-03-18 Novo Nordisk A/S Production of bispecific antibodies
EP2059533B1 (en) 2006-08-30 2012-11-14 Genentech, Inc. Multispecific antibodies
MX2009007632A (es) * 2007-01-22 2009-07-24 Genentech Inc Precipitacion de polielectrolito y purificacion de proteinas.
SI2235064T1 (sl) 2008-01-07 2016-04-29 Amgen Inc. Metoda za izdelavo heterodimernih molekul - protitelesa fc z uporabo elektrostatičnih usmerjevalnih učinkov
CN105274170A (zh) * 2009-11-05 2016-01-27 弗·哈夫曼-拉罗切有限公司 分泌异源多肽的方法和组合物
AU2010201410B2 (en) * 2010-03-30 2015-04-30 Pelican Technology Holdings, Inc. High level expression of recombinant CRM197
GB201012519D0 (en) 2010-07-26 2010-09-08 Ucl Business Plc Method and system for anomaly detection in data sets
GB201012599D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
MX2014005330A (es) * 2011-11-02 2014-09-08 Genentech Inc Cromatografia de sobrecarga y elucion.
SG10202108497TA (en) * 2013-04-19 2021-09-29 Sutro Biopharma Inc Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones
KR102332302B1 (ko) * 2013-09-13 2021-12-01 제넨테크, 인크. 세포주에서 숙주 세포 단백질 및 재조합 폴리펩티드 생성물을 검출 및 정량화하기 위한 조성물 및 방법
ES2761726T3 (es) 2015-03-06 2020-05-20 Hoffmann La Roche DsbA y DsbC ultrapurificadas y procedimientos de preparación y uso de las mismas

Similar Documents

Publication Publication Date Title
JP2018516229A5 (enExample)
HRP20192217T1 (hr) ULTRA-PROČIŠĆENI DsbA I DsbC TE POSTUPCI NJIHOVE IZRADE I UPORABE
US10246484B2 (en) Method for purifying recombinant protein
Tarrant et al. Host cell protein adsorption characteristics during protein A chromatography
Gagnon et al. Nonspecific interactions of chromatin with immunoglobulin G and protein A, and their impact on purification performance
US20120283419A1 (en) Separation of protein monomers from aggregates by solid weak anion exchange support functionalized with amine moieties
Nfor et al. Multi‐dimensional fractionation and characterization of crude protein mixtures: toward establishment of a database of protein purification process development parameters
JP2015503524A5 (enExample)
EP2326658A1 (en) Enhanced protein aggregate removal with multimodal anion exchangers in the presence of protein-excluded zwitterions
CN107849541A (zh) 纯化腺病毒载体的方法
CN115135339A (zh) 一种从完整的aav衣壳中分离或去除空的aav衣壳的方法
JP2017519838A (ja) タンパク質の精製のための方法及び試薬
KR20220079910A (ko) 숙주-세포 단백질을 특성화하는 방법
RU2018121653A (ru) Улучшенное разделение белков при ионообменной хроматографии
Grönberg Ion exchange chromatography
De Vos et al. Production of full-length SARS-CoV-2 nucleocapsid protein from Escherichia coli optimized by native hydrophobic interaction chromatography hyphenated to multi-angle light scattering detection
Kröner et al. Analytical characterization of complex, biotechnological feedstocks by pH gradient ion exchange chromatography for purification process development
JP7320121B2 (ja) 眼科用タンパク質医薬品の精製方法(Refining method of ophthalmic protein pharmaceuticals)
CN113166732A (zh) 使用亲和色谱法的用于疫苗病毒的纯化方法
Morrison et al. Purification of monomeric mAb from associated aggregates using selective desorption chromatography in hydroxyapatite systems
Giacometti et al. Protein and peptide separations
Chen et al. Partition coefficient screening–An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case
Caramelo-Nunes et al. Dynamic binding capacity and specificity of 3, 8-diamino-6-phenylphenanthridine-Sepharose support for purification of supercoiled plasmid deoxyribonucleic acid
Chen et al. Resin and loading condition screening for effective and robust byproducts removal by anion exchange chromatography: a case study
Bicho et al. Application of ethylenediamine monolith to purify a hemagglutinin influenza deoxyribonucleic acid-based vaccine