EA035635B1 - Использование щелочных промывочных растворов во время хроматографии для удаления примесей - Google Patents

Использование щелочных промывочных растворов во время хроматографии для удаления примесей Download PDF

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Publication number
EA035635B1
EA035635B1 EA201792012A EA201792012A EA035635B1 EA 035635 B1 EA035635 B1 EA 035635B1 EA 201792012 A EA201792012 A EA 201792012A EA 201792012 A EA201792012 A EA 201792012A EA 035635 B1 EA035635 B1 EA 035635B1
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EA
Eurasian Patent Office
Prior art keywords
protein
chromatography
wash solution
antibody
alkaline
Prior art date
Application number
EA201792012A
Other languages
English (en)
Russian (ru)
Other versions
EA201792012A1 (ru
Inventor
Цзюэ Ван
Нил Е. Джаффе
Крина Патель
Original Assignee
Бристол-Маерс Сквибб Компани
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Бристол-Маерс Сквибб Компани filed Critical Бристол-Маерс Сквибб Компани
Publication of EA201792012A1 publication Critical patent/EA201792012A1/ru
Publication of EA035635B1 publication Critical patent/EA035635B1/ru

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
EA201792012A 2015-03-13 2016-03-11 Использование щелочных промывочных растворов во время хроматографии для удаления примесей EA035635B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562132974P 2015-03-13 2015-03-13
PCT/US2016/021984 WO2016149088A1 (en) 2015-03-13 2016-03-11 Use of alkaline washes during chromatography to remove impurities

Publications (2)

Publication Number Publication Date
EA201792012A1 EA201792012A1 (ru) 2018-01-31
EA035635B1 true EA035635B1 (ru) 2020-07-17

Family

ID=55699796

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201792012A EA035635B1 (ru) 2015-03-13 2016-03-11 Использование щелочных промывочных растворов во время хроматографии для удаления примесей

Country Status (13)

Country Link
US (2) US10695693B2 (enExample)
EP (2) EP3268100A1 (enExample)
JP (1) JP6807859B2 (enExample)
KR (1) KR102490956B1 (enExample)
CN (1) CN107636005B (enExample)
AU (2) AU2016233557B2 (enExample)
BR (1) BR112017018365A2 (enExample)
CA (1) CA2978095C (enExample)
EA (1) EA035635B1 (enExample)
IL (1) IL254372B (enExample)
MX (1) MX395730B (enExample)
SG (2) SG11201707330RA (enExample)
WO (1) WO2016149088A1 (enExample)

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EP3268100A1 (en) 2015-03-13 2018-01-17 Bristol-Myers Squibb Company Use of alkaline washes during chromatography to remove impurities
JP7479151B2 (ja) 2017-01-30 2024-05-08 リジェネロン・ファーマシューティカルズ・インコーポレイテッド クロマトグラフィーにおけるバイオバーデンを低減するための組成物および方法
KR102882390B1 (ko) * 2018-06-19 2025-11-05 브리스톨-마이어스 스큅 컴퍼니 크로마토그래피를 사용하여 단백질을 정제하는 방법
US12152056B2 (en) * 2018-08-17 2024-11-26 Regeneron Pharmaceuticals, Inc. Method and chromatography system for determining amount and purity of a multimeric protein
KR102270048B1 (ko) * 2020-07-10 2021-06-28 주식회사 녹십자 바리셀라 조스터 바이러스 표면 단백질 항원의 생산 방법
AU2019369421A1 (en) * 2018-10-31 2021-06-03 Regeneron Pharmaceuticals, Inc. Method and system of identifying and quantifying a protein
CN113166200B (zh) * 2018-12-21 2024-03-01 上海药明生物技术有限公司 一种提高蛋白a层析法去除聚集体的方法
BR112021011194A2 (pt) * 2019-01-16 2021-08-31 Regeneron Pharmaceuticals, Inc. Método e sistema de identificar e quantificar a fragmentação do anticorpo
CN109870428A (zh) * 2019-01-21 2019-06-11 江苏华泰疫苗工程技术研究有限公司 一种基于表面等离子共振技术的抗体定量检测方法
GB201904741D0 (en) * 2019-04-04 2019-05-22 Glaxosmithkline Ip Dev Ltd Novel process
IL291020B2 (en) 2019-09-24 2025-04-01 Regeneron Pharma Systems and methods for use in chromatography and regeneration
CN114599971A (zh) * 2019-10-14 2022-06-07 皮尔斯生物科技有限公司 肽纯化配制物和方法
US20240262928A1 (en) * 2020-03-27 2024-08-08 Haemalogix Pty Ltd Composition and Method
IL301572A (en) * 2020-10-02 2023-05-01 Lilly Co Eli Methods for reducing the protein content of the host cell in protein purification processes
TW202600582A (zh) * 2024-02-20 2026-01-01 美商艾克塞里克斯公司 用於蛋白質純化之方法

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EP0280578A2 (en) * 1987-02-26 1988-08-31 Dana-Farber Cancer Institute, Inc. Purification of LFA-3
US6172197B1 (en) * 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
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WO2008031020A2 (en) * 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography

Also Published As

Publication number Publication date
CN107636005B (zh) 2021-07-16
BR112017018365A2 (pt) 2018-04-10
US12157073B2 (en) 2024-12-03
SG11201707330RA (en) 2017-10-30
CA2978095C (en) 2022-02-15
IL254372B (en) 2022-05-01
AU2021221870A1 (en) 2021-09-23
US20180078876A1 (en) 2018-03-22
CA2978095A1 (en) 2016-09-22
CN107636005A (zh) 2018-01-26
AU2016233557B2 (en) 2021-06-24
IL254372A0 (en) 2017-11-30
EP3268100A1 (en) 2018-01-17
MX2017011121A (es) 2017-11-28
US10695693B2 (en) 2020-06-30
AU2016233557A1 (en) 2017-11-02
EA201792012A1 (ru) 2018-01-31
JP6807859B2 (ja) 2021-01-06
KR20170125948A (ko) 2017-11-15
KR102490956B1 (ko) 2023-01-19
WO2016149088A1 (en) 2016-09-22
EP4194071A1 (en) 2023-06-14
MX395730B (es) 2025-03-25
SG10201908027QA (en) 2019-10-30
US20200282332A1 (en) 2020-09-10
JP2018510867A (ja) 2018-04-19

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