MX341620B - Solucion y metodo de lavado para cromatografia de afinidad. - Google Patents
Solucion y metodo de lavado para cromatografia de afinidad.Info
- Publication number
- MX341620B MX341620B MX2013014032A MX2013014032A MX341620B MX 341620 B MX341620 B MX 341620B MX 2013014032 A MX2013014032 A MX 2013014032A MX 2013014032 A MX2013014032 A MX 2013014032A MX 341620 B MX341620 B MX 341620B
- Authority
- MX
- Mexico
- Prior art keywords
- affinity chromatography
- wash solution
- arginine
- eluate
- maintaining
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
Abstract
La presente invención se refiere a un método para la producción de una proteína de interés purificada utilizando una matriz de cromatografía de afinidad (AC) con la que se enlaza la proteína de interés, comprendiendo el método lavar la matriz de cromatografía de afinidad (AC) con una solución de lavado que comprende arginina o una molécula seleccionada del grupo que consiste en acetil-arginina, agmatina, ácido argínico, N-alfa-butiroil-L-arginina, y N-alfa-pivaloil-arginina, un pH de más de 8.0, en donde el lavado se lleva a cabo sin la presencia de una sal o reguladora del pH.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161492092P | 2011-06-01 | 2011-06-01 | |
| PCT/EP2012/060313 WO2012164046A1 (en) | 2011-06-01 | 2012-05-31 | Wash solution and method for affinity chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2013014032A MX2013014032A (es) | 2014-01-23 |
| MX341620B true MX341620B (es) | 2016-08-26 |
Family
ID=46384327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013014032A MX341620B (es) | 2011-06-01 | 2012-05-31 | Solucion y metodo de lavado para cromatografia de afinidad. |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US9663552B2 (es) |
| EP (2) | EP3428176A1 (es) |
| JP (2) | JP6366502B2 (es) |
| KR (1) | KR101953405B1 (es) |
| CN (1) | CN103596968B (es) |
| AU (1) | AU2012264626B2 (es) |
| BR (1) | BR112013030628B1 (es) |
| CA (1) | CA2834300C (es) |
| DK (1) | DK2714714T3 (es) |
| ES (1) | ES2688348T3 (es) |
| IL (1) | IL229237B (es) |
| LT (1) | LT2714714T (es) |
| MX (1) | MX341620B (es) |
| PL (1) | PL2714714T3 (es) |
| PT (1) | PT2714714T (es) |
| RU (1) | RU2609633C2 (es) |
| SG (1) | SG194460A1 (es) |
| WO (1) | WO2012164046A1 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2784278C (en) * | 2009-12-18 | 2019-02-26 | Novartis Ag | Wash solution and method for affinity chromatography |
| WO2015103545A1 (en) | 2014-01-03 | 2015-07-09 | Bio-Rad Laboratories, Inc. | Removal of impurities from protein a eluates |
| WO2016031932A1 (ja) * | 2014-08-27 | 2016-03-03 | 田辺三菱製薬株式会社 | アルカリ洗浄によるＦｃ領域を有するタンパク質の製造方法 |
| CN105566442A (zh) * | 2014-10-11 | 2016-05-11 | 江苏泰康生物医药有限公司 | 一种降低单克隆抗体生产中宿主细胞蛋白含量的亲和纯化工艺 |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| BR112017018365A2 (pt) | 2015-03-13 | 2018-04-10 | Bristol-Myers Squibb Company | uso de lavagens alcalinas durante cromatografia para remoção de impurezas |
| PL3337817T3 (pl) * | 2015-08-21 | 2022-01-17 | F. Hoffmann-La Roche Ag | Sposób zmniejszania zawartości białek komórek gospodarza w chromatografii powinowactwa |
| EP3353547A1 (en) | 2015-09-22 | 2018-08-01 | Delta TM Technologies | Designing customized protein-specific buffer systems |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| JP7106187B2 (ja) | 2016-05-11 | 2022-07-26 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックスを保存する方法 |
| CN109311948B (zh) | 2016-05-11 | 2022-09-16 | 思拓凡生物工艺研发有限公司 | 清洁和/或消毒分离基质的方法 |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| ES2874974T3 (es) | 2016-05-11 | 2021-11-05 | Cytiva Bioprocess R & D Ab | Matriz de separación |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| WO2018212556A1 (en) * | 2017-05-16 | 2018-11-22 | Cj Healthcare Corporation | A method for purifying an antibody or an antibody fragment thereof using affinity chromatography |
| EP3769083A1 (en) | 2018-03-21 | 2021-01-27 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbents, devices and methods |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01238534A (ja) * | 1988-03-17 | 1989-09-22 | Mitsui Toatsu Chem Inc | エンドトキシンの除去方法 |
| ATE421536T1 (de) | 2001-06-05 | 2009-02-15 | Genetics Inst Llc | Verfahren zur reinigung stark anionischer proteine |
| WO2004037203A2 (en) * | 2002-10-24 | 2004-05-06 | Enos Pharmaceuticals, Inc. | Sustained release l-arginine formulations and methods of manufacture and use |
| US8084032B2 (en) * | 2004-01-21 | 2011-12-27 | Ajinomoto Co., Inc. | Purification method which prevents denaturation of an antibody |
| DE602006003108D1 (de) | 2005-03-03 | 2008-11-27 | Ajinomoto Kk | Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin |
| CN101511387A (zh) * | 2006-09-08 | 2009-08-19 | 惠氏公司 | 使用亲和色谱法纯化蛋白质的精氨酸洗涤液 |
| CA2784278C (en) * | 2009-12-18 | 2019-02-26 | Novartis Ag | Wash solution and method for affinity chromatography |
| DK2526114T3 (da) * | 2010-01-22 | 2014-09-29 | Boehringer Ingelheim Int | Kromatografisk fremgangsmåde til oprensning af FC-indeholdende proteiner |
-
2012
- 2012-05-31 JP JP2014513198A patent/JP6366502B2/ja active Active
- 2012-05-31 SG SG2013075841A patent/SG194460A1/en unknown
- 2012-05-31 EP EP18177943.0A patent/EP3428176A1/en not_active Withdrawn
- 2012-05-31 CA CA2834300A patent/CA2834300C/en active Active
- 2012-05-31 CN CN201280027076.2A patent/CN103596968B/zh active Active
- 2012-05-31 WO PCT/EP2012/060313 patent/WO2012164046A1/en active Application Filing
- 2012-05-31 US US14/122,707 patent/US9663552B2/en active Active
- 2012-05-31 MX MX2013014032A patent/MX341620B/es active IP Right Grant
- 2012-05-31 PT PT12729907T patent/PT2714714T/pt unknown
- 2012-05-31 DK DK12729907.1T patent/DK2714714T3/en active
- 2012-05-31 RU RU2013156669A patent/RU2609633C2/ru active
- 2012-05-31 EP EP12729907.1A patent/EP2714714B1/en active Active
- 2012-05-31 PL PL12729907T patent/PL2714714T3/pl unknown
- 2012-05-31 ES ES12729907.1T patent/ES2688348T3/es active Active
- 2012-05-31 AU AU2012264626A patent/AU2012264626B2/en active Active
- 2012-05-31 KR KR1020137031437A patent/KR101953405B1/ko active IP Right Grant
- 2012-05-31 LT LTEP12729907.1T patent/LT2714714T/lt unknown
- 2012-05-31 BR BR112013030628-9A patent/BR112013030628B1/pt active IP Right Grant
-
2013
- 2013-11-04 IL IL229237A patent/IL229237B/en active IP Right Grant
-
2017
- 2017-04-06 JP JP2017076234A patent/JP2017160215A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA2834300A1 (en) | 2012-12-06 |
| US9663552B2 (en) | 2017-05-30 |
| KR20140029465A (ko) | 2014-03-10 |
| RU2013156669A (ru) | 2015-07-20 |
| AU2012264626B2 (en) | 2015-11-05 |
| MX2013014032A (es) | 2014-01-23 |
| PT2714714T (pt) | 2018-10-22 |
| CN103596968B (zh) | 2018-05-15 |
| EP3428176A1 (en) | 2019-01-16 |
| KR101953405B1 (ko) | 2019-02-28 |
| EP2714714A1 (en) | 2014-04-09 |
| CN103596968A (zh) | 2014-02-19 |
| JP2014515389A (ja) | 2014-06-30 |
| US20140094593A1 (en) | 2014-04-03 |
| JP2017160215A (ja) | 2017-09-14 |
| BR112013030628B1 (pt) | 2022-01-25 |
| JP6366502B2 (ja) | 2018-08-01 |
| LT2714714T (lt) | 2018-09-25 |
| RU2609633C2 (ru) | 2017-02-02 |
| DK2714714T3 (en) | 2018-09-24 |
| CA2834300C (en) | 2022-01-11 |
| AU2012264626A1 (en) | 2013-11-28 |
| IL229237B (en) | 2018-05-31 |
| SG194460A1 (en) | 2013-12-30 |
| PL2714714T3 (pl) | 2018-11-30 |
| IL229237A0 (en) | 2014-01-30 |
| EP2714714B1 (en) | 2018-06-20 |
| ES2688348T3 (es) | 2018-11-02 |
| BR112013030628A2 (pt) | 2016-09-06 |
| WO2012164046A1 (en) | 2012-12-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |