JP6807859B2 - クロマトグラフィーにおける不純物を取り除くためのアルカリ洗浄の使用 - Google Patents

クロマトグラフィーにおける不純物を取り除くためのアルカリ洗浄の使用 Download PDF

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JP6807859B2
JP6807859B2 JP2017548109A JP2017548109A JP6807859B2 JP 6807859 B2 JP6807859 B2 JP 6807859B2 JP 2017548109 A JP2017548109 A JP 2017548109A JP 2017548109 A JP2017548109 A JP 2017548109A JP 6807859 B2 JP6807859 B2 JP 6807859B2
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chromatography
protein
wash solution
interest
antibody
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JP2018510867A5 (enExample
JP2018510867A (ja
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ジュー・ワン
ニール・イー・ジャフィ
クリナ・パテル
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Bristol Myers Squibb Co
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Bristol Myers Squibb Co
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP2017548109A 2015-03-13 2016-03-11 クロマトグラフィーにおける不純物を取り除くためのアルカリ洗浄の使用 Active JP6807859B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562132974P 2015-03-13 2015-03-13
US62/132,974 2015-03-13
PCT/US2016/021984 WO2016149088A1 (en) 2015-03-13 2016-03-11 Use of alkaline washes during chromatography to remove impurities

Publications (3)

Publication Number Publication Date
JP2018510867A JP2018510867A (ja) 2018-04-19
JP2018510867A5 JP2018510867A5 (enExample) 2019-03-22
JP6807859B2 true JP6807859B2 (ja) 2021-01-06

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JP2017548109A Active JP6807859B2 (ja) 2015-03-13 2016-03-11 クロマトグラフィーにおける不純物を取り除くためのアルカリ洗浄の使用

Country Status (13)

Country Link
US (2) US10695693B2 (enExample)
EP (2) EP3268100A1 (enExample)
JP (1) JP6807859B2 (enExample)
KR (1) KR102490956B1 (enExample)
CN (1) CN107636005B (enExample)
AU (2) AU2016233557B2 (enExample)
BR (1) BR112017018365A2 (enExample)
CA (1) CA2978095C (enExample)
EA (1) EA035635B1 (enExample)
IL (1) IL254372B (enExample)
MX (1) MX395730B (enExample)
SG (2) SG11201707330RA (enExample)
WO (1) WO2016149088A1 (enExample)

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JP7479151B2 (ja) 2017-01-30 2024-05-08 リジェネロン・ファーマシューティカルズ・インコーポレイテッド クロマトグラフィーにおけるバイオバーデンを低減するための組成物および方法
KR102882390B1 (ko) * 2018-06-19 2025-11-05 브리스톨-마이어스 스큅 컴퍼니 크로마토그래피를 사용하여 단백질을 정제하는 방법
US12152056B2 (en) * 2018-08-17 2024-11-26 Regeneron Pharmaceuticals, Inc. Method and chromatography system for determining amount and purity of a multimeric protein
KR102270048B1 (ko) * 2020-07-10 2021-06-28 주식회사 녹십자 바리셀라 조스터 바이러스 표면 단백질 항원의 생산 방법
AU2019369421A1 (en) * 2018-10-31 2021-06-03 Regeneron Pharmaceuticals, Inc. Method and system of identifying and quantifying a protein
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Also Published As

Publication number Publication date
CN107636005B (zh) 2021-07-16
BR112017018365A2 (pt) 2018-04-10
US12157073B2 (en) 2024-12-03
SG11201707330RA (en) 2017-10-30
CA2978095C (en) 2022-02-15
IL254372B (en) 2022-05-01
AU2021221870A1 (en) 2021-09-23
US20180078876A1 (en) 2018-03-22
CA2978095A1 (en) 2016-09-22
CN107636005A (zh) 2018-01-26
AU2016233557B2 (en) 2021-06-24
IL254372A0 (en) 2017-11-30
EP3268100A1 (en) 2018-01-17
MX2017011121A (es) 2017-11-28
US10695693B2 (en) 2020-06-30
AU2016233557A1 (en) 2017-11-02
EA201792012A1 (ru) 2018-01-31
KR20170125948A (ko) 2017-11-15
KR102490956B1 (ko) 2023-01-19
WO2016149088A1 (en) 2016-09-22
EP4194071A1 (en) 2023-06-14
MX395730B (es) 2025-03-25
SG10201908027QA (en) 2019-10-30
EA035635B1 (ru) 2020-07-17
US20200282332A1 (en) 2020-09-10
JP2018510867A (ja) 2018-04-19

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