AR076288A1 - Purificacion de proteinas por precipitacion con citrato - Google Patents

Purificacion de proteinas por precipitacion con citrato

Info

Publication number
AR076288A1
AR076288A1 ARP100101236A ARP100101236A AR076288A1 AR 076288 A1 AR076288 A1 AR 076288A1 AR P100101236 A ARP100101236 A AR P100101236A AR P100101236 A ARP100101236 A AR P100101236A AR 076288 A1 AR076288 A1 AR 076288A1
Authority
AR
Argentina
Prior art keywords
citrate
precipitation
proteins
purification
protein
Prior art date
Application number
ARP100101236A
Other languages
English (en)
Inventor
Alahari Arunakumari
Ferreira Gisela Maria Marques
Original Assignee
Bristol Myers Squibb Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Publication of AR076288A1 publication Critical patent/AR076288A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Procedimientos para aislar proteínas en forma purificada de mezclas mediante precipitacion con citrato. Los procedimientos tienen la ventaja de que separan eficazmente una proteína de contaminantes de peso molecular bajo, incluyendo fragmentos o porciones de la proteína. Tales procedimientos son particularmente utiles para purificar anticuerpos a partir de mezclas que contienen fragmentos proteolíticos del anticuerpo y cadenas desparejadas.
ARP100101236A 2009-04-13 2010-04-13 Purificacion de proteinas por precipitacion con citrato AR076288A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US16892009P 2009-04-13 2009-04-13

Publications (1)

Publication Number Publication Date
AR076288A1 true AR076288A1 (es) 2011-06-01

Family

ID=42269676

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP100101236A AR076288A1 (es) 2009-04-13 2010-04-13 Purificacion de proteinas por precipitacion con citrato

Country Status (5)

Country Link
US (1) US8063189B2 (es)
EP (1) EP2419436A1 (es)
AR (1) AR076288A1 (es)
TW (1) TW201041899A (es)
WO (1) WO2010120739A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
WO2008033517A2 (en) 2006-09-13 2008-03-20 Abbott Laboratories Cell culture improvements
EP2921501A1 (en) 2008-10-20 2015-09-23 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
US8765415B2 (en) * 2009-11-17 2014-07-01 Medarex, L.L.C. Methods for enhanced protein production
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
CN114249831A (zh) * 2015-03-13 2022-03-29 诺夫免疫股份有限公司 纯化双特异性抗体的方法
CA3012694A1 (en) * 2016-02-03 2017-08-10 Plasma Technologies, Llc Methods for extracting proteins from a blood-based material
CN114341152A (zh) 2019-09-06 2022-04-12 比亚分离公司 用于降低生物制品的染色质含量的组合物和方法
US10815270B1 (en) * 2019-09-20 2020-10-27 Plasma Technologies, Llc Compositions and methods for high efficiency protein precipitation
AU2021414086A1 (en) 2020-12-28 2023-08-03 Plasma Technologies, Llc Systems and Methods for Process Scale Isolation of Immunoglobulin G

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4321192A (en) * 1980-01-10 1982-03-23 Ionics Incorporated Fractionation of protein mixtures by salt addition followed by dialysis treatment
US4835257A (en) * 1984-07-07 1989-05-30 Armour Pharma Gmbh Process for preparing gamma globulin suitable for intravenous administration using peg and a citrate buffer
US4977246A (en) * 1989-06-06 1990-12-11 Rorer Pharmaceutical Corporation High recovery process for antihemophilic factor
US5371198A (en) * 1991-12-16 1994-12-06 Novo Nordisk A/S Method for protection of proteolysis-susceptible protein during protein production in a fluid medium
DE19932782A1 (de) * 1999-07-14 2001-01-18 Biotest Pharma Gmbh Verfahren zur chromatographischen Fraktionierung von Plasma oder Serum, so erhaltene Präparate und deren Verwendung
US7411006B2 (en) 2000-10-23 2008-08-12 Shanbrom Technologies, Llc Enhanced production of blood clotting factors and fibrin fabric
US20060223988A1 (en) * 2005-04-05 2006-10-05 National Research Laboratories, Ltd. High Resolution Methods and Precipitating Reagents for Isolating Proteins from Proteinaceous Material
US7879332B2 (en) * 2005-09-01 2011-02-01 Plasma Technologies, Llc Ultra-high yield intravenous immune globulin preparation
WO2008100578A2 (en) * 2007-02-14 2008-08-21 Amgen Inc. Method of isolating antibodies by precipitation

Also Published As

Publication number Publication date
US20100261886A1 (en) 2010-10-14
EP2419436A1 (en) 2012-02-22
WO2010120739A1 (en) 2010-10-21
TW201041899A (en) 2010-12-01
US8063189B2 (en) 2011-11-22

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