JP2001520045A5 - - Google Patents
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- JP2001520045A5 JP2001520045A5 JP2000517090A JP2000517090A JP2001520045A5 JP 2001520045 A5 JP2001520045 A5 JP 2001520045A5 JP 2000517090 A JP2000517090 A JP 2000517090A JP 2000517090 A JP2000517090 A JP 2000517090A JP 2001520045 A5 JP2001520045 A5 JP 2001520045A5
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- protease variant
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- 102000033147 ERVK-25 Human genes 0.000 description 14
- 108091005771 Peptidases Proteins 0.000 description 14
- 239000004365 Protease Substances 0.000 description 14
- 238000006467 substitution reaction Methods 0.000 description 9
- 108090000787 Subtilisin Proteins 0.000 description 8
- 230000000875 corresponding Effects 0.000 description 7
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 3
- 125000000539 amino acid group Chemical group 0.000 description 2
- 229920003013 deoxyribonucleic acid Polymers 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000193422 Bacillus lentus Species 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
Description
【特許請求の範囲】
【請求項1】 Bacillus amyloliquefaciens スブチリシンの位置103に対応する残基位置においておよび位置236および245のうちの1以上においてアミノ酸を置き換え、 Bacillus amyloliquefaciens スブチリシンの位置1、3、4、8、10、12、13、16、17、18、19、20、21、22、24、27、33、37、38、42、43、48、55、57、58、61、62、68、72、75、76、77、78、79、86、87、89、97、98、99、101、102、104、106、107、109、111、114、116、117、119、121、123、126、128、130、131、133、134、137、140、141、142、146、147、158、159、160、166、167、170、173、174、177、181、182、183、184、185、188、192、194、198、203、204、205、206、209、210、211、212、213、214、215、216、217、218、222、224、227、228、230、232、237、238、240、242、243、244、246、247、248、249、251、252、253、254、255、256、257、258、259、260、261、262、263、265、268、269、270、271、272、274および275に対応する残基位置よりなる群から選択される残基において1以上のアミノ酸を置き換えることを含み;ここに、残基位置103に対応する位置における置換が残基位置76に対応する位置における置換と組み合わされる場合、Bacillus amyloliquefaciens スブチリシンの位置27、99、101、104、107、109、123、128、166、204、206、210、216、217、218、222、260、265または274に対応する残基位置以外の1以上の残基位置における置換もあることを特徴とするプロテアーゼ変異体。
【請求項2】 位置103および236におけるおよび位置1、9、12、61、62、68、76、97、98、101、102、104、109、130、131、159、183、185、205、209、210、211、212、213、215、217、230、232、248、252、257、260、270および275のうちの1以上におけるアミノ酸残基の置換を含む請求項1記載のプロテアーゼ変異体。
【請求項3】 位置103および245におけるおよび位置1、9、12、61、62、68、76、97、98、101、102、104、109、130、131、159、170、183、185、205、209、210、211、212、213、215、217、222、230、232、248、252、257、260、261、270および275のうちの1以上におけるアミノ酸残基の置換を含む請求項1記載のプロテアーゼ変異体。
【請求項4】 位置103、236および245におけるおよび位置1、9、12、61、62、68、76、97、98、101、102、104、109、130、131、159、183、185、205、209、210、211、212、213、215、217、230、232、243、248、252、257、260、270および275のうちの1以上におけるアミノ酸残基の置換を含む請求項1記載のプロテアーゼ変異体。
【請求項5】 位置159におけるアミノ酸残基の置換を含む請求項1から4いずれか1項記載のプロテアーゼ変異体。
【請求項6】 Bacillus スブチリシンに由来する請求項1記載のプロテアーゼ変異体。
【請求項7】 Bacillus lentus スブチリシンに由来する請求項6記載のプロテアーゼ変異体。
【請求項8】 請求項1記載のプロテアーゼ変異体をコードするDNA。
【請求項9】 請求項8記載のDNAをコードする発現ベクター。
【請求項10】 請求項9記載の発現ベクターで形質転換した宿主細胞。
【請求項11】 請求項1記載のプロテアーゼ変異体を含む清浄組成物。
【請求項12】 請求項1記載のプロテアーゼ変異体を含む動物飼料。
【請求項13】 請求項1記載のプロテアーゼ変異体を含むテキスタイルを処理するための組成物。
【請求項14】 Bacillus amyloliquefaciens スブチリシンの表1における位置に対応する残基位置よりなる群から選択される置換組を含む請求項1記載のプロテアーゼ変異体。
【請求項15】 Bacillus amyloliquefaciens スブチリシンの表3における位置に対応する残基位置よりなる群から選択される置換組を含む請求項14記載のプロテアーゼ変異体。
【請求項16】 Bacillus amyloliquefaciens スブチリシンの表2における位置に対応する残基位置よりなる群から選択される置換組を含む請求項14記載のプロテアーゼ変異体。
【請求項1】 Bacillus amyloliquefaciens スブチリシンの位置103に対応する残基位置においておよび位置236および245のうちの1以上においてアミノ酸を置き換え、 Bacillus amyloliquefaciens スブチリシンの位置1、3、4、8、10、12、13、16、17、18、19、20、21、22、24、27、33、37、38、42、43、48、55、57、58、61、62、68、72、75、76、77、78、79、86、87、89、97、98、99、101、102、104、106、107、109、111、114、116、117、119、121、123、126、128、130、131、133、134、137、140、141、142、146、147、158、159、160、166、167、170、173、174、177、181、182、183、184、185、188、192、194、198、203、204、205、206、209、210、211、212、213、214、215、216、217、218、222、224、227、228、230、232、237、238、240、242、243、244、246、247、248、249、251、252、253、254、255、256、257、258、259、260、261、262、263、265、268、269、270、271、272、274および275に対応する残基位置よりなる群から選択される残基において1以上のアミノ酸を置き換えることを含み;ここに、残基位置103に対応する位置における置換が残基位置76に対応する位置における置換と組み合わされる場合、Bacillus amyloliquefaciens スブチリシンの位置27、99、101、104、107、109、123、128、166、204、206、210、216、217、218、222、260、265または274に対応する残基位置以外の1以上の残基位置における置換もあることを特徴とするプロテアーゼ変異体。
【請求項2】 位置103および236におけるおよび位置1、9、12、61、62、68、76、97、98、101、102、104、109、130、131、159、183、185、205、209、210、211、212、213、215、217、230、232、248、252、257、260、270および275のうちの1以上におけるアミノ酸残基の置換を含む請求項1記載のプロテアーゼ変異体。
【請求項3】 位置103および245におけるおよび位置1、9、12、61、62、68、76、97、98、101、102、104、109、130、131、159、170、183、185、205、209、210、211、212、213、215、217、222、230、232、248、252、257、260、261、270および275のうちの1以上におけるアミノ酸残基の置換を含む請求項1記載のプロテアーゼ変異体。
【請求項4】 位置103、236および245におけるおよび位置1、9、12、61、62、68、76、97、98、101、102、104、109、130、131、159、183、185、205、209、210、211、212、213、215、217、230、232、243、248、252、257、260、270および275のうちの1以上におけるアミノ酸残基の置換を含む請求項1記載のプロテアーゼ変異体。
【請求項5】 位置159におけるアミノ酸残基の置換を含む請求項1から4いずれか1項記載のプロテアーゼ変異体。
【請求項6】 Bacillus スブチリシンに由来する請求項1記載のプロテアーゼ変異体。
【請求項7】 Bacillus lentus スブチリシンに由来する請求項6記載のプロテアーゼ変異体。
【請求項8】 請求項1記載のプロテアーゼ変異体をコードするDNA。
【請求項9】 請求項8記載のDNAをコードする発現ベクター。
【請求項10】 請求項9記載の発現ベクターで形質転換した宿主細胞。
【請求項11】 請求項1記載のプロテアーゼ変異体を含む清浄組成物。
【請求項12】 請求項1記載のプロテアーゼ変異体を含む動物飼料。
【請求項13】 請求項1記載のプロテアーゼ変異体を含むテキスタイルを処理するための組成物。
【請求項14】 Bacillus amyloliquefaciens スブチリシンの表1における位置に対応する残基位置よりなる群から選択される置換組を含む請求項1記載のプロテアーゼ変異体。
【請求項15】 Bacillus amyloliquefaciens スブチリシンの表3における位置に対応する残基位置よりなる群から選択される置換組を含む請求項14記載のプロテアーゼ変異体。
【請求項16】 Bacillus amyloliquefaciens スブチリシンの表2における位置に対応する残基位置よりなる群から選択される置換組を含む請求項14記載のプロテアーゼ変異体。
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US95632397A | 1997-10-23 | 1997-10-23 | |
US95632497A | 1997-10-23 | 1997-10-23 | |
US95656497A | 1997-10-23 | 1997-10-23 | |
US08/956,324 | 1997-10-23 | ||
US08/956,564 | 1997-10-23 | ||
US08/956,323 | 1997-10-23 | ||
PCT/US1998/022572 WO1999020770A2 (en) | 1997-10-23 | 1998-10-23 | Multiply-substituted protease variants |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2001520045A JP2001520045A (ja) | 2001-10-30 |
JP2001520045A5 true JP2001520045A5 (ja) | 2006-01-05 |
JP4511025B2 JP4511025B2 (ja) | 2010-07-28 |
Family
ID=27420734
Family Applications (7)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2000517089A Expired - Fee Related JP4346237B2 (ja) | 1997-10-23 | 1998-10-23 | 複数置換プロテアーゼ変異体 |
JP2000517091A Pending JP2001520046A (ja) | 1997-10-23 | 1998-10-23 | 洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体 |
JP2000517090A Expired - Fee Related JP4511025B2 (ja) | 1997-10-23 | 1998-10-23 | 複数置換プロテアーゼ変異体 |
JP2000517046A Pending JP2001520305A (ja) | 1997-10-23 | 1998-10-23 | 多置換プロテアーゼ変種およびアミラーゼ変種を含有したクリーニング組成物 |
JP2000517049A Pending JP2001520307A (ja) | 1997-10-23 | 1998-10-23 | 多置換プロテアーゼ変種を含有した漂白組成物 |
JP2000517050A Pending JP2001520308A (ja) | 1997-10-23 | 1998-10-23 | 多置換プロテアーゼ変種を含有したクリーニング組成物 |
JP2012138496A Expired - Fee Related JP5612026B2 (ja) | 1997-10-23 | 2012-06-20 | 洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体 |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2000517089A Expired - Fee Related JP4346237B2 (ja) | 1997-10-23 | 1998-10-23 | 複数置換プロテアーゼ変異体 |
JP2000517091A Pending JP2001520046A (ja) | 1997-10-23 | 1998-10-23 | 洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体 |
Family Applications After (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2000517046A Pending JP2001520305A (ja) | 1997-10-23 | 1998-10-23 | 多置換プロテアーゼ変種およびアミラーゼ変種を含有したクリーニング組成物 |
JP2000517049A Pending JP2001520307A (ja) | 1997-10-23 | 1998-10-23 | 多置換プロテアーゼ変種を含有した漂白組成物 |
JP2000517050A Pending JP2001520308A (ja) | 1997-10-23 | 1998-10-23 | 多置換プロテアーゼ変種を含有したクリーニング組成物 |
JP2012138496A Expired - Fee Related JP5612026B2 (ja) | 1997-10-23 | 2012-06-20 | 洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体 |
Country Status (26)
Country | Link |
---|---|
US (5) | US6482628B1 (ja) |
EP (10) | EP1027418B2 (ja) |
JP (7) | JP4346237B2 (ja) |
KR (6) | KR20010031380A (ja) |
CN (9) | CN1195857C (ja) |
AR (10) | AR015977A1 (ja) |
AT (9) | ATE417099T1 (ja) |
AU (7) | AU1276299A (ja) |
BR (6) | BR9813879A (ja) |
CA (9) | CA2307638C (ja) |
CZ (6) | CZ20001479A3 (ja) |
DE (7) | DE69840346D1 (ja) |
DK (9) | DK1025239T3 (ja) |
EG (2) | EG21711A (ja) |
ES (7) | ES2241180T5 (ja) |
HK (1) | HK1039159B (ja) |
HU (3) | HUP0104539A3 (ja) |
ID (4) | ID25897A (ja) |
MA (2) | MA24811A1 (ja) |
NO (3) | NO326992B1 (ja) |
NZ (3) | NZ520771A (ja) |
PL (1) | PL191529B1 (ja) |
PT (8) | PT1025239E (ja) |
TR (8) | TR200001112T2 (ja) |
TW (2) | TW585912B (ja) |
WO (5) | WO1999020770A2 (ja) |
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2001
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2002
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2006
- 2006-01-11 AR ARP060100109A patent/AR052282A2/es not_active Application Discontinuation
- 2006-01-11 AR ARP060100110A patent/AR052453A2/es unknown
- 2006-01-11 AR ARP060100107A patent/AR052281A2/es not_active Application Discontinuation
- 2006-01-11 AR ARP060100108A patent/AR052353A2/es active IP Right Grant
- 2006-01-11 AR ARP060100111A patent/AR052354A2/es not_active Application Discontinuation
- 2006-01-11 AR ARP060100112A patent/AR052355A2/es not_active Application Discontinuation
-
2012
- 2012-06-20 JP JP2012138496A patent/JP5612026B2/ja not_active Expired - Fee Related
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