RU2011123911A - Композиции, содержащие варианты сериновых протеаз, и способы - Google Patents

Композиции, содержащие варианты сериновых протеаз, и способы Download PDF

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RU2011123911A
RU2011123911A RU2011123911/10A RU2011123911A RU2011123911A RU 2011123911 A RU2011123911 A RU 2011123911A RU 2011123911/10 A RU2011123911/10 A RU 2011123911/10A RU 2011123911 A RU2011123911 A RU 2011123911A RU 2011123911 A RU2011123911 A RU 2011123911A
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subtilisin
variant
positions
analysis
specified
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Вольфганг Эле
Луис Г. КАСКАО-ПЕРЕЙРА
Дэвид А. ЭСТЕЛЛ
Фриц ГУДЕГЕБУР
Джеймс Т. Мл. КЕЛЛИС
Айрукаран Дж. ПАУЛОЗ
Брайан Ф. ШМИДТ
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ДАНИСКО ЮЭс ИНК.
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Abstract

1. Выделенный вариант субтилизина, где указанный вариант субтилизина представляет собой зрелую форму, имеющую протеолитическую активность и содержащую замены в трех или более положений, выбранных из: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274 и 275, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN', указанной в качестве SEQ ID NO: 1.2. Выделенный вариант субтилизинапо п.1, где указанный вариант субтилизина представляет собой зрелую форму, имеющую протеолитическую активность и содержащую замены в трех или более положений, выбранных из: 1, 3, 4, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 33, 35, 36, 38, 40, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 61, 62, 68, 69, 72, 73, 76, 78, 84, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 114, 115, 116, 117, 118, 119, 120, 121, 122, 124, 126, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 156, 158, 159, 160, 165, 166, 167, 170, 171, 172, 173, 174, 175, 176, 177, 180, 182, 183, 184, 185, 186, 187, 188, 191, 194, 195, 198, 199, 203, 204, 206, 209, 210, 211, 212, 213, 215, 216, 217, 218, 222, 223, 224, 227, 228, 230, 231, 232, 233, 234,

Claims (33)

1. Выделенный вариант субтилизина Bacillus, где указанный вариант субтилизина представляет собой зрелую форму, имеющую протеолитическую активность и содержащую замены в трех или более положений, выбранных из: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274 и 275, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
2. Выделенный вариант субтилизина Bacillus по п.1, где указанный вариант субтилизина представляет собой зрелую форму, имеющую протеолитическую активность и содержащую замены в трех или более положений, выбранных из: 1, 3, 4, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 33, 35, 36, 38, 40, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 61, 62, 68, 69, 72, 73, 76, 78, 84, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 114, 115, 116, 117, 118, 119, 120, 121, 122, 124, 126, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 156, 158, 159, 160, 165, 166, 167, 170, 171, 172, 173, 174, 175, 176, 177, 180, 182, 183, 184, 185, 186, 187, 188, 191, 194, 195, 198, 199, 203, 204, 206, 209, 210, 211, 212, 213, 215, 216, 217, 218, 222, 223, 224, 227, 228, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 255, 256, 258, 259, 260, 261, 262, 265, 267, 268, 269, 270, 271, 272, 273, 274 и 275, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
3. Выделенный вариант субтилизина по п.1, где указанный вариант субтилизина представляет собой зрелую форму, имеющую протеолитическую активность и содержащую замены в трех или более положений, выбранных из: 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 33, 35, 36, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 66, 68, 69, 71, 72, 73, 74, 75, 76, 77, 78, 79, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 156, 158, 159, 160, 165, 166, 167, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 191, 192, 194, 195, 196, 197, 198, 199, 200, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 220, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 267, 268, 269, 270, 271, 272, 273, 274 и 275, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
4. Выделенный вариант субтилизина по п.1, где указанные замены в трех или более положениях выбраны из: X001A, X001C, X001E, X001F, X001G, X001H, X001I, X001K, X001L, X001N, X001P, X001Q, X001R, X001S, X001T, X001V, X001Y, X002A, X002C, X002E, X002G, X002K, X002L, X002M, X002N, X002P, X002Q, X002R, X002S, X002T, X002V, X002W, X002Y, X003D, X003E, X003F, X003G, X003H, X003I, X003L, X003M, X003N, X003P, X003R, X003S, X003T, X003V, X003W, X003Y, X004A, X004C, X004D, X004E, X004F, X004G, X004H, X004K, X004L, X004N, X004P, X004R, X004S, X004T, X004V, X004W, X005A, X005C, X005D, X005E, X005G, X005I, X005M, X005P, X005Q, X005S, X005T, X005W, X005Y, X006A, X006D, X006E, X006M, X006W, X007A, X007C, X007D, X007G, X007H, X007N, X007P, X007Q, X007S, X007T, X008A, X008F, X008G, X008I, X008L, X008M, X008Q, X008T, X008V, X008W, X008Y, X009A, X009C, X009D, X009E, X009F, X009G, X009H, X009L, X009N, X009P, X009Q, X009R, X009S, X009T, X009V, X009W, X009Y, X010A, X010C, X010F, X010G, X010H, X010I, X010K, 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X140A, X140C, X140D, X140E, X140F, X140G, X140I, X140K, X140L, X140M, X140N, X140Q, X140R, X140S, X140T, X140V, X140Y, X141D, X141E, X141G, X141H, X141I, X141K, X141L, X141N, X141Q, X141R, X141S, X141V, X141Y, X142A, X142C, X142E, X142G, X142I, X142L, X142M, X142N, X142Q, X142S, X142T, X142V, X142Y, X143C, X143D, X143F, X143G, X143H, X143I, X143K, X143L, X143M, X143N, X143R, X143S, X143T, X143V, X143W, X143Y, X144A, X144C, X144D, X144G, X144H, X144I, X144L, X144M, X144N, X144Q, X144R, X144S, X144T, X144V, X144W, X144Y, X145A, X145C, X145D, X145E, X145F, X145G, X145K, X145L, X145M, X145N, X145Q, X145R, X145S, X145T, X145W, X145Y, X146A, X146C, X146D, X146E, X146F, X146G, X146I, X146K, X146L, X146M, X146Q, X146R, X146S, X146T, X146W, X146Y, X147F, X147G, X147I, X147L, X147M, X147P, X147Q, X147T, X147V, X148A, X148C, X148E, X148F, X148G, X148H, X148I, X148L, X148M, X148N, X148S, X148T, X148V, X148W, X148Y, X149A, X149C, X149F, X149G, X149H, X149I, X149L, X149M, X149P, X149Q, X149S, X149T, 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X165V, X165W, X165Y, X166A, X166C, X166D, X166E, X166F, X166H, X166I, X166L, X166M, X166N, X166P, X166R, X166S, X166T, X166V, X166W, X166Y, X167A, X167C, X167D, X167E, X167F, X167G, X167H, X167I, X167K, X167L, X167M, X167N, X167P, X167Q, X167R, X167S, X167T, X167V, X167W, X167Y, X168A, X168C, X168F, X168I, X168M, X168N, X168P, X168S, X168T, X168V, X169A, X169C, X169G, X169I, X169L, X169S, X170A, X170D, X170E, X170G, X170H, X170K, X170L, X170N, X170P, X170Q, X170R, X170S, X170V, X170W, X170Y, X171A, X171C, X171D, X171E, X171F, X171G, X171H, X171I, X171L, X171M, X171N, X171Q, X171S, X171T, X171V, X171W, X171Y, X172A, X172C, X172D, X172F, X172G, X172I, X172K, X172L, X172M, X172P, X172Q, X172R, X172S, X172T, X172V, X172W, X172Y, X173A, X173C, X173D, X173E, X173F, X173G, X173H, X173I, X173K, X173L, X173M, X173N, X173P, X173Q, X173R, X173T, X173V, X173W, X173Y, X174A, X174G, X174I, X174N, X174P, X174S, X174T, X174V, X175A, X175C, X175E, X175G, X175H, X175I, X175K, X175L, X175M, X175Q, X175S, X175T, X175V, X175W, X175Y, X176A, X176C, X176E, X176G, X176I, X176K, X176P, X176S, X176T, X176V, X177A, X177C, X177I, X177T, X177V, X178A, X178G, X178S, X178T, X179A, X179C, X179G, X179H, X179I, X180C, X180G, X180H, X180I, X180L, X180N, X180Q, X180S, X180T, X180V, X181A, X181D, X181E, X181G, X181H, X181L, X181M, X181N, X182A, X182D, X182E, X182F, X182G, X182H, X182I, X182K, X182L, X182M, X182N, X182P, X182Q, X182R, X182S, X182T, X182V, X182W, X182Y, X183A, X183D, X183F, X183G, X183H, X183I, X183K, X183L, X183M, X183N, X183P, X183Q, X183R, X183S, X183T, X183V, X183W, X183Y, X184A, X184C, X184D, X184E, X184F, X184G, X184H, X184L, X184M, X184N, X184S, X184T, X184W, X184Y, X185A, X185C, X185E, X185F, X185G, X185H, X185I, X185K, X185L, X185M, X185N, X185Q, X185R, X185S, X185T, X185V, X185Y, X186A, X186C, X186G, X186H, X186I, X186K, X186L, X186M, X186N, X186P, X186Q, X186R, X186S, X186T, X186W, X187A, X187C, X187D, X187E, X187F, X187G, X187H, X187I, X187L, X187M, X187N, X187P, X187Q, X187S, X187T, X187V, X187W, X187Y, X188A, X188D, X188E, X188F, X188G, X188H, X188I, X188K, X188L, X188P, X188Q, X188R, X188S, X188T, X188V, X188W, X188Y, X189A, X189C, X189E, X189F, X189G, X189H, X189K, X189L, X189M, X189N, X189P, X189Q, X189R, X189S, X189T, X189V, X189Y, X190A, X190D, X190E, X190F, X190G, X190H, X190I, X190K, X190L, X190M, X190N, X190P, X190Q, X190R, X190S, X190V, X190W, X190Y, X191A, X191D, X191E, X191F, X191H, X191I, X191K, X191L, X191P, X191Q, X191R, X191S, X191T, X191V, X191W, X191Y, X192C, X192D, X192E, X192G, X192H, X192I, X192K, X192L, X192M, X192N, X192P, X192Q, X192R, X192S, X192T, X192V, X192W, X192Y, X193A, X193D, X193E, X193F, X193G, X193H, X193I, X193K, X193L, X193R, X193S, X193T, X193V, X193W, X193Y, X194A, X194C, X194D, X194E, X194F, X194G, X194H, X194I, X194L, X194M, X194P, X194Q, X194R, X194S, X194T, X194V, X194W, X194Y, X195A, X195C, X195D, X195E, X195F, X195G, X195I, X195K, X195L, X195Q, X195R, X195S, X195T, X195V, X195W, X195Y, X196A, X196D, X196E, X196F, X196I, X196L, X196M, X196P, X196Q, X196T, X196V, X196Y, X197A, X197C, X197D, X197E, X197F, X197G, X197H, X197I, X197L, X197M, X197N, X197Q, X197R, X197S, X197T, X197V, X197W, X197Y, X198A, X198D, X198E, X198F, X198G, X198H, X198I, X198L, X198M, X198N, X198Q, X198R, X198S, X198T, X198W, X198Y, X199A, X199C, X199D, X199E, X199F, X199G, X199H, X199I, X199L, X199M, X199Q, X199S, X199T, X199V, X199W, X200A, X200C, X200G, X200H, X200I, X200S, X201C, X201G, X201P, X201S, X201T, X201V, X202F, X202G, X203A, X203C, X203E, X203F, X203G, X203H, X203I, X203K, X203L, X203N, X203R, X203S, X203T, X203V, X203W, X203Y, X204A, X204C, X204E, X204F, X204G, X204I, X204K, X204L, X204N, X204P, X204R, X204S, X204T, X204W, X204Y, X205A, X205F, X205G, X205I, X205L, X205M, X205Q, X205T, X205V, X206A, X206C, X206D, X206E, X206F, X206G, X206H, X206I, X206K, X206L, X206N, X206P, X206Q, X206R, X206S, X206T, X206V, X206W, X206Y, X207A, X207G, X207H, X207S, X208A, X208C, X208L, X208N, X208P, X208S, X208T, X208V, X209A, X209C, X209D, X209E, X209F, X209G, X209H, X209I, X209K, X209L, X209M, X209N, X209R, X209S, X209T, X209V, X209W, X209Y, X210A, X210C, X210D, X210E, X210F, X210G, X210H, X210I, X210L, X210M, X210N, X210P, X210Q, X210R, X210S, X210V, X210W, X210Y, X211A, X211C, X211E, X211F, X211G, X211H, X211I, X211L, X211M, X211P, X211Q, X211R, X211T, X211V, X211W, X211Y, X212C, X212F, X212G, X212H, X212I, X212M, X212N, X212P, X212R, X212S, X212T, X212V, X212Y, X213A, X213C, X213D, X213E, X213F, X213G, X213I, X213K, X213L, X213M, X213N, X213P, X213Q, X213R, X213S, X213T, X213V, X213W, X213Y, X214A, X214C, X214E, X214F, X214G, X214H, X214I, X214K, X214L, X214M, X214N, X214P, X214Q, X214R, X214S, X214T, X214V, X214W, X214Y, X215A, X215C, X215D, X215E, X215F, X215G, X215H, X215I, X215K, X215M, X215N, X215P, X215R, X215S, X215T, X215V, X215W, X215Y, X216A, X216C, X216D, X216E, X216F, X216G, X216H, X216I, X216K, X216L, X216M, X216N, X216P, X216Q, X216R, X216S, X216V, X216W, X216Y, X217A, X217C, X217D, X217E, X217F, X217G, X217I, X217K, X217L, X217M, X217N, X217Q, X217S, X217T, X217V, X217Y, X218C, X218D, X218E, X218F, X218G, X218H, X218I, X218L, X218M, X218N, X218P, X218Q, X218R, X218S, X218T, X218V, X218W, X218Y, X219A, X219G, X219S, X220A, X220C, X220G, X220H, X220N, X220S, X220T, X220V, X221G, X221S, X221T, X222A, X222C, X222E, X222F, X222G, X222I, X222K, X222L, X222M, X222N, X222P, X222Q, X222R, X222S, X222T, X222V, X222W, X223A, X223C, X223G, X223M, X223S, X223T, X224A, X224D, X224E, X224G, X224I, X224L, X224M, X224N, X224P, X224S, X224T, X225A, X225C, X225G, X225I, X225N, X225P, X225S, X225T, X225V, X226C, X226F, X226G, X226H, X226I, X226L, X226M, X226N, X226R, X226S, X226T, X226V, X226Y, X227A, X227C, X227F, X227G, X227I, X227L, X227M, X227P, X227Q, X227S, X227T, X227V, X227Y, X228A, X228C, X228G, X228I, X228L, X228M, X228P, X228S, X228V, X229A, X229G, X229P, X229S, X230A, X230D, X230E, X230F, X230G, X230H, X230I, X230L, X230N, X230P, X230Q, X230S, X230T, X230V, X230W, X230Y, X231A, X231C, X231F, X231G, X231H, X231I, X231L, X231S, X231T, X231W, X231Y, X232A, X232G, X232H, X232K, X232L, X232M, X232P, X232S, X232V, X233A, X233C, X233E, X233F, X233G, X233I, X233L, X233M, X233N, X233P, X233Q, X233R, X233S, X233T, X233V, X233Y, X234D, X234F, X234G, X234H, X234L, X234M, X234N, X234P, X234Q, X234S, X234T, X234V, X234Y, X235C, X235D, X235E, X235F, X235G, X235H, X235I, X235K, X235L, X235M, X235N, X235Q, X235R, X235S, X235V, X235W, X235Y, X236A, X236C, X236E, X236F, X236G, X236H, X236K, X236L, X236N, X236P, X236Q, X236R, X236S, X236T, X236V, X236W, X236Y, X237A, X237C, X237F, X237G, X237H, X237I, X237K, X237L, X237M, X237P, X237Q, X237R, X237S, X237T, X237V, X237W, X237Y, X238C, X238D, X238E, X238F, X238G, X238H, X238I, X238K, X238L, X238M, X238N, X238Q, X238R, X238S, X238T, X238V, X238Y, X239C, X239D, X239F, X239G, X239H, X239I, X239K, X239L, X239M, X239N, X239P, X239Q, X239R, X239S, X239T, X239V, X239W, X239Y, X240A, X240C, X240E, X240F, X240I, X240K, X240L, X240M, X240N, X240Q, X240R, X240S, X240T, X240W, X240Y, X241A, X241C, X241D, X241E, X241F, X241G, X241H, X241I, X241K, X241L, X241M, X241N, X241P, X241Q, X241R, X241S, X241T, X241V, X241W, X241Y, X242A, X242C, X242D, X242F, X242G, X242H, X242I, X242L, X242M, X242P, X242Q, X242R, X242S, X242T, X242V, X242W, X243C, X243D, X243E, X243F, X243G, X243H, X243I, X243K, X243L, X243M, X243N, X243P, X243Q, X243R, X243S, X243T, X243V, X243W, X244A, X244D, X244E, X244F, X244H, X244I, X244L, X244M, X244N, X244P, X244Q, X244R, X244S, X244T, X244V, X244W, X244Y, X245A, X245C, X245E, X245G, X245H, X245K, X245L, X245P, X245Q, X245R, X245S, X245V, X245W, X245Y, X246A, X246C, X246E, X246F, X246G, X246H, X246I, X246L, X246M, X246N, X246P, X246Q, X246R, X246S, X246T, X246V, X246W, X246Y, X247A, X247C, X247D, X247E, X247F, X247G, X247H, X247I, X247K, X247L, X247M, X247N, X247P, X247Q, X247R, X247S, X247T, X247V, X247W, X247Y, X248C, X248D, X248E, X248G, X248H, X248I, X248K, X248L, X248M, X248N, X248P, X248R, X248S, X248T, X248V, X248W, X248Y, X249A, X249D, X249E, X249F, X249G, X249H, X249I, X249K, X249L, X249M, X249N, X249Q, X249R, X249S, X249T, X249V, X249W, X249Y, X250A, X250C, X250E, X250F, X250G, X250H, X250I, X250L, X250M, X250N, X250Q, X250S, X250V, X250Y, X251A, X251D, X251E, X251F, X251G, X251K, X251L, X251M, X251P, X251Q, X251R, X251S, X251T, X251V, X251Y, X252A, X252C, X252D, X252F, X252G, X252H, X252I, X252K, X252L, X252M, X252N, X252P, X252R, X252S, X252V, X252W, X252Y, X253A, X253D, X253E, X253F, X253G, X253H, X253I, X253K, X253M, X253R, X253S, X253T, X253V, X253W, X254A, X254C, X254D, X254E, X254G, X254N, X254P, X254S, X254T, X254V, X255A, X255C, X255D, X255E, X255F, X255H, X255I, X255L, X255N, X255P, X255Q, X255R, X255S, X255T, X255V, X255W, X255Y, X256A, X256C, X256D, X256E, X256G, X256H, X256I, X256K, X256L, X256M, X256N, X256P, X256R, X256S, X256T, X256V, X256W, X256Y, X257A, X257C, X257E, X257F, X257G, X257H, X257I, X257K, X257L, X257M, X257P, X257S, X257T, X257V, X257W, X257Y, X258A, X258C, X258D, X258E, X258F, X258G, X258H, X258I, X258L, X258M, X258P, X258Q, X258R, X258S, X258T, X258V, X258W, X258Y, X259A, X259C, X259E, X259G, X259I, X259L, X259M, X259P, X259Q, X259R, X259S, X259T, X259V, X260A, X260D, X260E, X260F, X260H, X260I, X260L, X260M, X260N, X260P, X260R, X260S, X260T, X260V, X260Y, X261A, X261C, X261E, X261F, X261G, X261I, X261K, X261L, X261N, X261P, X261Q, X261R, X261S, X261T, X261V, X261W, X261Y, X262A, X262C, X262D, X262F, X262G, X262H, X262I, X262K, X262L, X262M, X262P, X262Q, X262R, X262S, X262T, X262V, X262W, X262Y, X263A, X263C, X263D, X263F, X263G, X263H, X263I, X263K, X263L, X263M, X263N, X263P, X263Q, X263R, X263W, X263Y, X264A, X264C, X264E, X264F, X264G, X264H, X264I, X264L, X264P, X264Q, X264S, X264T, X264V, X264Y, X265A, X265C, X265D, X265F, X265G, X265H, X265I, X265K, X265L, X265M, X265N, X265P, X265Q, X265R, X265S, X265T, X265V, X265W, X265Y, X266G, X266W, X266Y, X267A, X267C, X267D, X267E, X267F, X267G, X267H, X267I, X267K, X267L, X267M, X267N, X267S, X267T, X267V, X267Y, X268A, X268D, X268E, X268G, X268H, X268K, X268L, X268M, X268N, X268P, X268Q, X268R, X268S, X268V, X268W, X268Y, X269C, X269D, X269F, X269G, X269H, X269I, X269L, X269M, X269N, X269Q, X269R, X269S, X269T, X269V, X270A, X270C, X270D, X270E, X270F, X270G, X270H, X270I, X270L, X270M, X270N, X270P, X270Q, X270S, X270T, X270V, X271A, X271C, X271E, X271F, X271G, X271H, X271I, X271K, X271L, X271M, X271N, X271P, X271T, X271V, X271Y, X272A, X272C, X272D, X272E, X272F, X272G, X272H, X272K, X272L, X272M, X272N, X272P, X272R, X272S, X272T, X272W, X272Y, X273A, X273C, X273D, X273E, X273F, X273G, X273H, X273I, X273K, X273L, X273R, X273S, X273T, X273V, X273W, X273Y, X274A, X274C, X274D, X274E, X274G, X274H, X274K, X274L, X274M, X274N, X274P, X274Q, X274R, X274S, X274T, X274W, X275A, X275C, X275D, X275E, X275F, X275G, X275H, X275K, X275L, X275M, X275P, X275Q, X275R, X275V и X275W, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
5. Выделенный вариант субтилизина, имеющий показатель эффективности более 1 по меньшей мере в одном анализе, выбранном из анализа с BMI, анализа микрообразцов тканей с пятном яичного желтка и/или анализа активности в отношении AAPF, и показатель эффективности более 0,8 в анализе устойчивости к LAS или в анализе с TCA, где указанный вариант содержит по меньшей мере одну замену в одном или более положениях, выбранных из: 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 33, 35, 36, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 66, 68, 69, 71, 72, 73, 74, 75, 76, 77, 78, 79, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 156, 158, 159, 160, 165, 166, 167, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 191, 192, 194, 195, 196, 197, 198, 199, 200, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 220, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 267, 268, 269, 270, 271, 272, 273, 274 и 275, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
6. Выделенный вариант субтилизина, имеющий показатель эффективности более 0,8 по меньшей мере в одном анализе, выбранном из анализа с BMI, анализа микрообразцов ткани с пятном яичного желтка и/или в анализе активности в отношении AAPF, и показатель эффективности более 0,8 в анализе устойчивости к LAS и в анализе с TCA, где указанный вариант содержит по меньшей мере одну замену в одном или более положениях, выбранных из: 1, 3, 4, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 33, 35, 36, 38, 40, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 61, 62, 68, 69, 72, 73, 76, 78, 84, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 114, 115, 116, 117, 118, 119, 120, 121, 122, 124, 126, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 156, 158, 159, 160, 165, 166, 167, 170, 171, 172, 173, 174, 175, 176, 177, 180, 182, 183, 184, 185, 186, 187, 188, 191, 194, 195, 198, 199, 203, 204, 206, 209, 210, 211, 212, 213, 215, 216, 217, 218, 222, 223, 224, 227, 228, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 255, 256, 258, 259, 260, 261, 262, 265, 267, 268, 269, 270, 271, 272, 273, 274 и 275, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
7. Выделенный вариант субтилизина по п.5, где указанный выделенный вариант имеет показатель эффективности более 1 по меньшей мере в одном анализе, выбранном из анализа с BMI, анализа микрообразцов тканей с пятном яичного желтка и/или анализа активности в отношении AAPF, и показатель эффективности более 0,8 для анализа устойчивости к LAS/EDTA или анализе с TCA, где вариант содержит по меньшей мере три замены, выбранные из: X001A, X001C, X001E, X001F, X001G, X001H, X001I, X001K, X001L, X001N, X001Q, X001R, X001S, X001T, X001V, X001Y, X002A, X002C, X002E, X002G, X002K, X002L, X002M, X002N, X002P, X002Q, X002R, X002S, X002T, X002V, X002W, X002Y, X003D, X003E, X003F, X003G, X003H, X003I, X003L, X003M, X003N, X003P, X003R, X003S, X003T, X003V, X003W, X003Y, X004A, X004C, X004D, X004E, X004F, X004H, X004K, X004L, X004N, X004P, X004R, X004S, X004T, X004V, X004W, X005A, X005C, X005D, X005E, X005G, X005P, X005Q, X005S, X005T, X007A, X007C, X007D, X007G, X007H, X007N, X007S, X007T, X008A, X008F, X008I, X008L, X008M, X008T, X008V, X009D, X009E, X009F, X009G, X009H, X009L, X009N, X009P, X009Q, X009R, X009S, X009T, X009V, X009W, X009Y, X010A, X010C, X010G, X010H, X010K, X010M, X010N, X010Q, X010R, X010S, X010T, X010W, X011A, X011I, X011M, X011S, X011V, X012D, X012F, X012G, X012H, X012I, X012K, X012L, X012M, X012N, X012Q, X012R, X012S, X012T, X012V, X012W, X013A, X013G, X013I, X013T, X013V, X014A, X014D, X014E, X014F, X014H, X014I, X014K, X014L, X014P, X014Q, X014S, X014T, X014V, X014Y, X015A, X015D, X015F, X015G, X015I, X015K, X015L, X015M, X015P, X015Q, X015R, X015S, X015V, X015W, X016A, X016G, X016L, X016N, X016P, X016Q, X016S, X016T, X016V, X017A, X017F, X017H, X017I, X017K, X017M, X017N, X017R, X017S, X017V, X017W, X017Y, X018A, X018C, X018D, X018E, X018F, X018G, X018H, X018K, X018L, X018M, X018N, X018P, X018Q, X018R, X018S, X018T, X018V, X018W, X018Y, X019A, X019C, X019D, X019E, X019F, X019G, X019H, X019K, X019L, X019M, X019N, X019R, X019S, X019T, X019V, X019W, X019Y, X020A, X020C, X020D, X020F, X020G, X020I, X020K, X020L, X020M, X020P, X020Q, X020R, X020S, X020T, X020V, X020W, X020Y, X021A, X021C, X021D, X021E, X021G, X021H, X021I, X021K, X021L, X021M, X021N, X021P, X021Q, X021R, X021S, X021T, X021V, X021W, X022A, X022C, X022G, X022I, X022K, X022L, X022M, X022N, X022P, X022Q, X022R, X022S, X022T, X022V, X022W, X022Y, X023A, X023G, X023S, X024A, X024C, X024D, X024F, X024G, X024H, X024L, X024M, X024N, X024P, X024Q, X024R, X024S, X024T, X024V, X024W, X025C, X025D, X025E, X025F, X025G, X025H, X025K, X025L, X025M, X025N, X025Q, X025R, X025S, X025T, X025V, X025W, X026C, X026F, X026G, X026I, X026L, X026M, X026N, X026P, X026R, X026S, X026T, X026V, X027A, X027C, X027D, X027F, X027G, X027I, X027K, X027L, X027M, X027N, X027P, X027R, X027S, X027T, X027V, X027W, X027Y, X028A, X028E, X028H, X028I, X028L, X028M, X028N, X028S, X028V, X029A, X029C, X029G, X029S, X029V, X030C, X030E, X030L, X030S, X030T, X030V, X031A, X031F, X031I, X031L, X031M, X031S, X031T, X031V, X033A, X033D, X033E, X033G, X033H, X033M, X033N, X033Q, X033S, X033T, X033Y, X035A, X035F, X035I, X035L, X035M, X035P, X036A, X036C, X036E, X036F, X036G, X036H, X036I, X036L, X036M, X036N, X036Q, X036R, X036S, X036T, X036V, X038C, X038F, X038G, X038H, X038I, X038K, X038L, X038M, X038N, X038Q, X038R, X038T, X038V, X038W, X038Y, X039H, X039V, X040A, X040C, X040D, X040E, X040G, X040H, X040I, X040K, X040L, X040M, X040N, X040P, X040R, X040S, X040T, X040V, X040W, X040Y, X041D, X041E, X042C, X042H, X042I, X042L, X042M, X042N, X042T, X042V, X043A, X043C, X043D, X043E, X043F, X043G, X043I, X043L, X043M, X043N, X043P, X043R, X043S, X043T, X043V, X043W, X043Y, X044A, X044C, X044D, X044G, X044I, X044K, X044L, X044M, X044N, X044P, X044Q, X044R, X044S, X044T, X044V, X044W, X044Y, X045A, X045D, X045F, X045G, X045H, X045I, X045K, X045L, X045M, X045N, X045P, X045Q, X045R, X045S, X045T, X045V, X045W, X045Y, X046C, X046D, X046E, X046F, X046G, X046H, X046I, X046K, X046L, X046M, X046N, X046P, X046Q, X046R, X046S, X046T, X046V, X046W, X047A, X047F, X047G, X047N, X047R, X047S, X047T, X047W, X048A, X048C, X048E, X048F, X048G, X048H, X048I, X048K, X048L, X048M, X048N, X048P, X048Q, X048R, X048S, X048T, X048V, X048Y, X049A, X049F, X049G, X049L, X049M, X049P, X049S, X049T, X050C, X050F, X050H, X050I, X050L, X050N, X050T, X050V, X050Y, X051F, X051G, X051H, X051K, X051L, X051N, X051P, X051R, X051S, X051T, X051V, X051W, X052A, X052C, X052E, X052F, X052G, X052H, X052I, X052L, X052M, X052N, X052P, X052Q, X052R, X052T, X052V, X052W, X052Y, X053A, X053C, X053D, X053E, X053G, X053H, X053K, X053L, X053M, X053P, X053Q, X053R, X053S, X053T, X053V, X053W, X053Y, X054A, X054C, X054D, X054E, X054F, X054I, X054M, X054N, X054Q, X054S, X054V, X055A, X055C, X055E, X055F, X055G, X055H, X055I, X055K, X055L, X055M, X055N, X055P, X055Q, X055S, X055T, X055W, X055Y, X056A, X056C, X056D, X056E, X056H, X056L, X056M, X056N, X056P, X056Q, X056S, X056T, X057A, X057C, X057E, X057F, X057G, X057H, X057I, X057K, X057L, X057M, X057N, X057P, X057Q, X057R, X057S, X057T, X057V, X057W, X057Y, X059A, X059C, X059D, X059E, X059F, X059G, X059I, X059L, X059M, X059N, X059P, X059Q, X059R, X059S, X059T, X059V, X059W, X059Y, X060D, X060S, X060V, X061A, X061C, X061D, X061F, X061G, X061H, X061I, X061L, X061M, X061N, X061P, X061R, X061S, X061T, X061V, X061Y, X062C, X062E, X062F, X062G, X062H, X062I, X062K, X062L, X062M, X062N, X062P, X062Q, X062R, X062S, X062T, X062V, X062Y, X063A, X063C, X063D, X063E, X063F, X063G, X063H, X063K, X063M, X063Q, X063R, X063S, X063W, X066K, X066S, X066T, X068I, X068T, X068V, X069A, X069E, X069G, X069N, X069S, X069T, X071A, X071I, X071N, X071T, X071V, X072C, X072F, X072I, X072L, X072M, X072T, X072V, X073A, X073C, X073D, X073E, X073H, X073K, X073L, X073N, X073S, X073T, X073V, X074A, X074C, X074S, X075A, X075H, X075I, X075L, X075M, X075N, X075P, X075Q, X075R, X075S, X075V, X076D, X076E, X076F, X076G, X076H, X076K, X076M, X076N, X076Q, X076R, X076S, X076T, X076W, X076Y, X077D, X077N, X078A, X078C, X078E, X078F, X078G, X078H, X078I, X078K, X078L, X078M, X078N, X078P, X078Q, X078R, X078S, X078T, X078V, X078Y, X079C, X079D, X079E, X079F, X079G, X079I, X079K, X079L, X079N, X079Q, X079R, X079S, X079T, X079V, X079W, X079Y, X081C, X081G, X081H, X081I, X081L, X081M, X081Q, X081S, X081T, X081V, X082A, X082E, X082F, X082K, X082L, X082M, X082Q, X082R, X082T, X082V, X082Y, X083G, X083S, X084C, X084E, X084G, X084I, X084L, X084M, X084N, X084T, X084V, X085A, X085C, X085I, X086A, X086C, X086D, X086E, X086G, X086P, X086S, X086W, X086Y, X087A, X087C, X087D, X087E, X087F, X087G, X087I, X087K, X087L, X087N, X087S, X087T, X087V, X087Y, X088A, X088C, X088D, X088G, X088Q, X088S, X088W, X089A, X089C, X089D, X089E, X089F, X089G, X089H, X089I, X089L, X089N, X089P, X089Q, X089R, X089S, X089T, X089V, X089W, X090A, X090C, X090F, X090G, X090I, X090K, X090L, X090M, X090Q, X090T, X090V, X091C, X091D, X091F, X091I, X091K, X091L, X091M, X091N, X091Q, X091R, X091S, X091T, X091V, X091W, X091Y, X092A, X092D, X092G, X092N, X092P, X092R, X092T, X092V, X093A, X093C, X093G, X093L, X093M, X093S, X093T, X093V, X093Y, X094K, X094N, X094Q, X094R, X095A, X095C, X095G, X095I, X095K, X095R, X095S, X095T, X095V, X095W, X096E, X096I, X096L, X096M, X096T, X097A, X097D, X097E, X097G, X097H, X097I, X097K, X097L, X097M, X097N, X097P, X097Q, X097R, X097S, X097T, X097V, X097Y, X098A, X098C, X098D, X098E, X098F, X098G, X098K, X098L, X098N, X098P, X098Q, X098R, X098S, X098T, X098V, X098Y, X099A, X099C, X099F, X099G, X099K, X099M, X099P, X099Q, X099R, X099S, X099T, X099V, X099Y, X100D, X100E, X100G, X100I, X100P, X100S, X100V, X100Y, X101A, X101D, X101E, X101F, X101G, X101H, X101I, X101K, X101N, X101P, X101Q, X101R, X101S, X101T, X101V, X101Y, X102A, X102C, X102D, X102G, X102N, X102T, X103A, X103D, X103E, X103F, X103G, X103I, X103L, X103N, X103P, X103Q, X103R, X103S, X103T, X103V, X103Y, X104A, X104C, X104D, X104E, X104F, X104H, X104I, X104L, X104P, X104R, X104S, X104T, X104V, X104W, X104Y, X105A, X105C, X105D, X105E, X105F, X105G, X105H, X105I, X105K, X105L, X105N, X105Q, X105R, X105S, X105T, X105V, X105W, X105Y, X106A, X106D, X106E, X106F, X106G, X106I, X106L, X106M, X106P, X106R, X106S, X106V, X106W, X107A, X107F, X107I, X107L, X107M, X107Q, X107S, X107T, X107V, X108A, X108G, X108I, X108L, X108S, X108T, X108V, X109A, X109C, X109E, X109F, X109G, X109H, X109I, X109K, X109L, X109M, X109N, X109Q, X109R, X109S, X109T, X109W, X109Y, X110A, X110G, X110S, X111C, X111E, X111F, X111I, X111L, X111M, X111V, X111Y, X112A, X112C, X112D, X112E, X112F, X112G, X112I, X112L, X112N, X112Q, X112S, X112T, X112V, X112W, X112Y, X114A, X114C, X114G, X114T, X115C, X115E, X115F, X115G, X115H, X115I, X115K, X115L, X115M, X115N, X115P, X115Q, X115R, X115S, X115T, X115V, X115W, X115Y, X116A, X116C, X116D, X116F, X116G, X116I, X116K, X116L, X116M, X116N, X116Q, X116S, X116T, X116V, X116W, X117A, X117C, X117D, X117F, X117G, X117I, X117N, X117Q, X117R, X117T, X117Y, X118A, X118C, X118D, X118E, X118F, X118G, X118I, X118K, X118L, X118M, X118N, X118P, X118R, X118S, X118T, X118V, X118W, X119A, X119C, X119F, X119H, X119M, X119N, X119Q, X119T, X119W, X120A, X120C, X120E, X120F, X120G, X120H, X120I, X120L, X120M, X120N, X120R, X120S, X120T, X120W, X121A, X121C, X121E, X121F, X121G, X121I, X121L, X121M, X121Q, X121S, X121T, X121V, X121Y, X122A, X122C, X122G, X122I, X122L, X122S, X122T, X122V, X123G, X123N, X123S, X124G, X124L, X124S, X124T, X125A, X125S, X126A, X126F, X126L, X127F, X127G, X127I, X127R, X127S, X127T, X128A, X128F, X128G, X128I, X128K, X128L, X128M, X128N, X128Q, X128R, X128S, X128T, X128W, X129A, X129E, X129F, X129G, X129I, X129L, X129M, X129N, X129P, X129R, X129S, X129T, X129V, X129W, X129Y, X130C, X130K, X130L, X130N, X130Q, X130R, X130S, X130V, X130W, X130Y, X131A, X131D, X131E, X131F, X131G, X131I, X131K, X131L, X131P, X131Q, X131R, X131V, X132A, X132E, X132F, X132H, X132I, X132L, X132M, X132N, X132Q, X132S, X132T, X132W, X133A, X133F, X133K, X133L, X133N, X133P, X133Q, X133S, X133T, X133V, X133Y, X134A, X134F, X134I, X134L, X134M, X134P, X134S, X134T, X134V, X135C, X135E, X135L, X135M, X135W, X136A, X136E, X136F, X136K, X136Q, X136R, X136S, X136V, X136W, X136Y, X137A, X137C, X137E, X137G, X137H, X137K, X137L, X137M, X137Q, X137R, X137S, X137V, X137W, X138A, X138G, X138M, X138R, X138V, X139A, X139C, X139I, X139M, X139T, X139V, X140A, X140C, X140D, X140E, X140F, X140G, X140I, X140L, X140M, X140N, X140Q, X140R, X140S, X140T, X140V, X140Y, X141D, X141E, X141G, X141H, X141I, X141K, X141L, X141N, X141Q, X141R, X141S, X141V, X141Y, X142A, X142C, X142L, X142T, X142V, X142Y, X143C, X143D, X143F, X143G, X143H, X143I, X143K, X143L, X143M, X143N, X143S, X143T, X143V, X143W, X143Y, X144A, X144C, X144D, X144G, X144H, X144I, X144L, X144M, X144N, X144Q, X144R, X144S, X144T, X144V, X144W, X144Y, X145A, X145C, X145D, X145E, X145F, X145G, X145K, X145L, X145M, X145N, X145Q, X145R, X145S, X145T, X145W, X145Y, X146A, X146C, X146D, X146E, X146G, X146K, X146Q, X147I, X147L, X147M, X147T, X147V, X148A, X148C, X148E, X148F, X148H, X148I, X148L, X148M, X148N, X148S, X148T, X148V, X148Y, X149A, X149C, X149I, X149L, X149M, X149P, X149S, X149T, X149V, X150A, X150F, X150L, X150T, X150V, X151A, X151G, X151S, X152A, X152S, X153A, X153G, X153S, X153V, X156A, X156C, X156D, X156E, X156F, X156L, X156M, X156N, X156S, X156T, X158A, X158C, X158E, X158F, X158H, X158K, X158L, X158M, X158Q, X158R, X158S, X158T, X158V, X158W, X159A, X159C, X159E, X159G, X159H, X159M, X159P, X159Q, X159R, X159S, X159W, X160A, X160C, X160D, X160F, X160G, X160I, X160L, X160M, X160N, X160Q, X160R, X160S, X160T, X160V, X160Y, X165I, X165L, X165T, X165V, X166A, X166C, X166D, X166E, X166H, X166M, X166N, X166S, X167C, X167D, X167E, X167F, X167P, X167V, X167W, X167Y, X169A, X169G, X169S, X170A, X170D, X170E, X170G, X170H, X170K, X170L, X170P, X170Q, X170R, X170S, X170V, X170W, X170Y, X171C, X171F, X171L, X171N, X171Y, X172A, X172C, X172D, X172F, X172G, X172I, X172K, X172L, X172M, X172P, X172Q, X172R, X172S, X172T, X172V, X172Y, X173A, X173C, X173D, X173E, X173F, X173G, X173H, X173K, X173L, X173M, X173N, X173Q, X173R, X173T, X173V, X173W, X174A, X174G, X174S, X174T, X174V, X175A, X175C, X175I, X175L, X175M, X175Q, X175T, X175V, X175Y, X176A, X176G, X176S, X177A, X177C, X177I, X177T, X177V, X178A, X178G, X179A, X179G, X180C, X180I, X180L, X180S, X180T, X180V, X181D, X181N, X182A, X182D, X182E, X182F, X182G, X182H, X182I, X182K, X182L, X182M, X182N, X182P, X182Q, X182R, X182S, X182T, X182V, X182W, X182Y, X183A, X183D, X183F, X183G, X183H, X183I, X183K, X183L, X183M, X183N, X183Q, X183R, X183S, X183T, X183V, X183W, X183Y, X184D, X184N, X185A, X185C, X185E, X185F, X185G, X185H, X185I, X185K, X185L, X185M, X185N, X185Q, X185R, X185S, X185T, X185V, X185Y, X186H, X186I, X186K, X186L, X186R, X187A, X187P, X187W, X188A, X188D, X188E, X188F, X188G, X188H, X188I, X188K, X188L, X188P, X188Q, X188R, X188S, X188T, X188V, X188W, X188Y, X191D, X191Q, X192W, X192Y, X194A, X194C, X194D, X194E, X194F, X194G, X194H, X194I, X194L, X194M, X194P, X194Q, X194R, X194S, X194T, X194V, X194W, X194Y, X195A, X195C, X195D, X195E, X195G, X195Q, X195Y, X196L, X196M, X197A, X197C, X197D, X197E, X197G, X197N, X197Q, X197S, X197T, X198A, X198F, X198G, X198H, X198I, X198L, X198M, X198N, X198R, X198S, X198T, X198Y, X199A, X199C, X199I, X199M, X199S, X199T, X199V, X200A, X200C, X200G, X200S, X203A, X203C, X203E, X203I, X203L, X203S, X203T, X203V, X204A, X204C, X204E, X204F, X204G, X204I, X204K, X204L, X204N, X204R, X204S, X204T, X204W, X204Y, X205I, X205T, X205V, X206A, X206C, X206D, X206E, X206G, X206H, X206I, X206K, X206L, X206N, X206P, X206Q, X206R, X206S, X206T, X206V, X206W, X206Y, X207A, X207S, X208C, X208L, X208S, X208T, X208V, X209A, X209C, X209F, X209G, X209H, X209I, X209K, X209L, X209M, X209N, X209R, X209S, X209V, X209W, X209Y, X210A, X210C, X210E, X210G, X210H, X210I, X210L, X210M, X210N, X210P, X210R, X210S, X210V, X210Y, X211A, X211C, X211E, X211F, X211G, X211H, X211I, X211L, X211M, X211P, X211Q, X211R, X211T, X211V, X211W, X211Y, X212C, X212F, X212G, X212H, X212I, X212M, X212N, X212P, X212R, X212S, X212T, X212V, X212Y, X213A, X213C, X213D, X213E, X213F, X213G, X213I, X213K, X213L, X213M, X213N, X213Q, X213R, X213S, X213T, X213V, X213W, X213Y, X214F, X214L, X214W, X214Y, X215A, X215C, X215D, X215E, X215F, X215G, X215H, X215I, X215K, X215M, X215N, X215P, X215R, X215S, X215T, X215V, X215W, X215Y, X216A, X216C, X216D, X216E, X216F, X216G, X216H, X216I, X216K, X216L, X216M, X216N, X216P, X216Q, X216R, X216S, X216V, X216W, X216Y, X217A, X217C, X217E, X217F, X217G, X217I, X217K, X217L, X217M, X217Q, X218C, X218D, X218E, X218F, X218G, X218H, X218I, X218M, X218N, X218Q, X218R, X218S, X218T, X218V, X218Y, X220S, X220T, X222A, X222M, X222Q, X223A, X223G, X223S, X224A, X224G, X224L, X224N, X224S, X224T, X225C, X225P, X226C, X226F, X226H, X226M, X226V, X227A, X227C, X227G, X227I, X227L, X227M, X227S, X227T, X227V, X228A, X228C, X228G, X228I, X228S, X228V, X229A, X229G, X229P, X229S, X230A, X230D, X230E, X230G, X230H, X230I, X230L, X230N, X230Q, X230S, X230T, X230V, X231A, X231C, X231F, X231G, X231H, X231I, X231L, X231S, X231T, X231Y, X232A, X232G, X232H, X232L, X232M, X232S, X232V, X233A, X233C, X233E, X233F, X233G, X233I, X233L, X233M, X233N, X233P, X233Q, X233S, X233T, X233V, X233Y, X234D, X234F, X234G, X234H, X234L, X234M, X234N, X234Q, X234S, X234T, X234V, X234Y, X235C, X235D, X235E, X235F, X235G, X235H, X235I, X235K, X235L, X235M, X235N, X235Q, X235R, X235S, X235V, X235W, X235Y, X236A, X236C, X236E, X236F, X236H, X236K, X236N, X236P, X236Q, X236R, X236S, X236T, X236V, X236W, X236Y, X237A, X237C, X237F, X237G, X237H, X237I, X237K, X237L, X237M, X237P, X237Q, X237R, X237S, X237T, X237V, X237W, X237Y, X238C, X238D, X238E, X238F, X238G, X238H, X238I, X238K, X238L, X238M, X238N, X238Q, X238R, X238S, X238T, X238V, X238Y, X239C, X239D, X239F, X239G, X239H, X239I, X239K, X239L, X239M, X239N, X239P, X239Q, X239R, X239S, X239T, X239V, X239W, X239Y, X240A, X240C, X240E, X240F, X240I, X240K, X240L, X240M, X240N, X240Q, X240R, X240S, X240T, X240W, X240Y, X241A, X241C, X241D, X241E, X241F, X241G, X241H, X241I, X241K, X241L, X241M, X241N, X241P, X241Q, X241R, X241S, X241T, X241V, X241W, X241Y, X242A, X242C, X242D, X242F, X242G, X242H, X242I, X242L, X242M, X242P, X242Q, X242R, X242S, X242T, X242V, X242W, X243C, X243D, X243E, X243F, X243G, X243H, X243I, X243K, X243L, X243M, X243N, X243P, X243Q, X243R, X243S, X243T, X243V, X243W, X244A, X244D, X244E, X244F, X244H, X244I, X244L, X244M, X244N, X244P, X244Q, X244R, X244S, X244T, X244V, X244W, X244Y, X245A, X245C, X245E, X245G, X245H, X245K, X245L, X245P, X245Q, X245R, X245S, X245V, X245W, X245Y, X246A, X246C, X246E, X246F, X246G, X246H, X246I, X246L, X246M, X246N, X246Q, X246S, X246T, X246V, X246W, X246Y, X247A, X247C, X247D, X247E, X247F, X247G, X247H, X247I, X247K, X247L, X247M, X247N, X247P, X247Q, X247R, X247S, X247T, X247V, X247W, X247Y, X248C, X248D, X248E, X248G, X248H, X248I, X248K, X248L, X248N, X248P, X248R, X248S, X248T, X248V, X248W, X248Y, X249A, X249D, X249E, X249F, X249G, X249H, X249I, X249K, X249L, X249M, X249N, X249Q, X249R, X249S, X249T, X249V, X249W, X249Y, X250A, X250C, X250F, X250I, X250L, X250M, X250Q, X250S, X250V, X251A, X251D, X251E, X251F, X251G, X251K, X251L, X251M, X251Q, X251R, X251T, X251V, X251Y, X252A, X252C, X252D, X252F, X252G, X252H, X252I, X252K, X252L, X252M, X252N, X252R, X252S, X252V, X252W, X252Y, X253A, X253D, X253E, X253F, X253G, X253H, X253I, X253K, X253M, X253R, X253S, X253T, X253V, X253W, X254A, X254C, X254G, X254S, X254T, X255A, X255C, X255D, X255E, X255F, X255H, X255I, X255L, X255N, X255P, X255Q, X255R, X255S, X255T, X255V, X255W, X255Y, X256A, X256C, X256D, X256E, X256G, X256H, X256I, X256K, X256L, X256M, X256N, X256P, X256R, X256S, X256T, X256V, X256W, X256Y, X257C, X257F, X257I, X257K, X257L, X257M, X257V, X258A, X258C, X258D, X258E, X258F, X258G, X258H, X258I, X258L, X258M, X258P, X258Q, X258R, X258S, X258T, X258V, X258W, X258Y, X259A, X259C, X259E, X259G, X259I, X259L, X259M, X259P, X259Q, X259R, X259S, X259T, X259V, X260A, X260D, X260E, X260F, X260H, X260I, X260L, X260M, X260N, X260P, X260R, X260S, X260T, X260V, X260Y, X261A, X261C, X261E, X261F, X261G, X261I, X261K, X261L, X261N, X261P, X261Q, X261R, X261S, X261T, X261V, X261W, X261Y, X262A, X262C, X262D, X262F, X262G, X262H, X262I, X262K, X262L, X262M, X262Q, X262R, X262S, X262T, X262V, X262W, X262Y, X263C, X263F, X263L, X263Y, X264A, X264G, X265A, X265C, X265D, X265F, X265G, X265H, X265K, X265M, X265Q, X265R, X265S, X265T, X265W, X265Y, X267G, X267I, X267L, X267M, X267N, X267V, X268A, X268G, X268H, X268L, X268M, X268N, X268P, X268Q, X268S, X268V, X269C, X269D, X269F, X269G, X269H, X269I, X269L, X269M, X269N, X269Q, X269R, X269S, X269T, X269V, X270A, X270C, X270D, X270G, X270I, X270L, X270M, X270N, X270S, X270T, X270V, X271A, X271C, X271E, X271F, X271G, X271H, X271I, X271K, X271L, X271M, X271N, X271P, X271T, X271V, X271Y, X272A, X272C, X272D, X272E, X272F, X272G, X272H, X272K, X272L, X272M, X272N, X272P, X272R, X272S, X272T, X272W, X272Y, X273A, X273C, X273D, X273E, X273F, X273G, X273H, X273I, X273L, X273S, X273T, X273V, X274A, X274C, X274D, X274E, X274G, X274H, X274L, X274M, X274N, X274P, X274Q, X274R, X274S, X274T, X274W, X275A, X275C, X275D, X275E, X275F, X275G, X275H, X275K, X275L, X275M, X275P, X275Q, X275R, X275V и X275W, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
8. Выделенный вариант субтилизина по п.6, где указанный вариант имеет показатель эффективности более 1 по меньшей мере в одном анализе, выбранном из анализа с BMI, анализа микрообразцов тканей с пятном яичного желтка и/или анализа активности в отношении AAPF, и показатель эффективности более 0,8 в анализе устойчивости к LAS и в анализе с TCA, где указанный вариант содержит по меньшей мере три замены, выбранные из: X001A, X001E, X001G, X001H, X001Q, X001V, X003E, X003H, X003I, X003M, X003S, X003T, X003V, X004T, X004V, X008I, X008V, X009E, X009H, X009N, X009Q, X009S, X009T, X010A, X010C, X010G, X010H, X010K, X010M, X010N, X010R, X010S, X010T, X011I, X011V, X012G, X012I, X012N, X012Q, X012S, X012T, X012V, X013A, X013G, X015A, X015D, X015F, X015G, X015I, X015L, X015M, X015P, X015Q, X015S, X015V, X015W, X016A, X016G, X016N, X016P, X016S, X016T, X016V, X017H, X017I, X017M, X017N, X018A, X018C, X018D, X018E, X018G, X018H, X018L, X018M, X018N, X018P, X018Q, X018S, X018T, X018V, X018Y, X019A, X019C, X019D, X019E, X019F, X019G, X019H, X019K, X019L, X019M, X019N, X019R, X019S, X019V, X019W, X019Y, X020A, X020C, X020D, X020F, X020G, X020I, X020K, X020L, X020M, X020P, X020Q, X020R, X020S, X020T, X020V, X020W, X020Y, X021A, X021C, X021E, X021G, X021H, X021I, X021K, X021L, X021M, X021N, X021P, X021Q, X021S, X021T, X021V, X021W, X022A, X022C, X022G, X022I, X022L, X022M, X022N, X022P, X022Q, X022T, X022V, X022W, X023A, X023G, X023S, X024A, X024C, X024D, X024F, X024G, X024H, X024L, X024M, X024N, X024P, X024Q, X024R, X024S, X024T, X024V, X024W, X025C, X025D, X025E, X025F, X025G, X025K, X025L, X025M, X025Q, X025R, X025S, X025T, X025V, X025W, X026C, X026F, X026G, X026M, X026N, X026R, X026S, X026T, X026V, X027A, X027C, X027D, X027F, X027G, X027K, X027L, X027M, X027N, X027P, X027R, X027S, X027T, X027W, X028A, X028I, X028L, X028M, X028V, X029A, X029C, X029G, X029S, X029V, X030A, X030C, X030L, X030M, X030T, X030V, X031A, X031F, X031I, X031L, X031M, X031S, X031V, X033C, X033M, X033S, X033T, X035I, X035L, X035M, X035P, X036A, X036E, X036S, X036T, X036V, X038C, X038F, X038H, X038I, X038K, X038L, X038M, X038N, X038Q, X038R, X038T, X038V, X038Y, X040A, X040D, X040E, X040I, X040L, X040P, X040V, X043A, X043C, X043D, X043E, X043F, X043G, X043I, X043L, X043M, X043N, X043R, X043S, X043T, X043W, X043Y, X044A, X044C, X044D, X044F, X044G, X044I, X044K, X044L, X044M, X044N, X044Q, X044R, X044S, X044T, X044V, X044Y, X045A, X045D, X045F, X045G, X045H, X045I, X045K, X045L, X045M, X045N, X045P, X045Q, X045R, X045S, X045T, X045V, X045W, X045Y, X046C, X046D, X046E, X046G, X046H, X046I, X046K, X046L, X046M, X046N, X046P, X046Q, X046R, X046S, X046T, X046V, X047G, X047R, X048A, X048C, X048E, X048F, X048H, X048I, X048K, X048L, X048M, X048N, X048P, X048Q, X048R, X048S, X048T, X048V, X048Y, X049A, X049G, X049H, X049S, X049T, X050F, X050H, X050I, X050L, X050T, X050V, X050Y, X051F, X051H, X051K, X051L, X051R, X051S, X051T, X051V, X051W, X052A, X052C, X052E, X052F, X052G, X052H, X052I, X052L, X052M, X052N, X052P, X052Q, X052R, X052T, X052V, X052W, X052Y, X053A, X053C, X053D, X053E, X053G, X053H, X053K, X053L, X053M, X053Q, X053R, X053S, X053T, X053W, X053Y, X054A, X054C, X054D, X054E, X054F, X054H, X054M, X054N, X054Q, X054S, X055C, X055E, X055G, X055H, X055K, X055L, X055P, X055Q, X055S, X055T, X055W, X056A, X056C, X056D, X056E, X056H, X056L, X056M, X056N, X056P, X056Q, X056S, X056T, X057C, X057E, X057F, X057G, X057H, X057I, X057L, X057M, X057N, X057P, X057Q, X057R, X057S, X057T, X057V, X057W, X057Y, X059A, X059D, X059G, X059I, X059L, X059M, X059N, X059Q, X059R, X059S, X059T, X059V, X059W, X061A, X061C, X061D, X061F, X061G, X061H, X061I, X061L, X061M, X061N, X061P, X061R, X061S, X061T, X061V, X061Y, X062C, X062E, X062F, X062H, X062I, X062K, X062L, X062M, X062N, X062Q, X062R, X062S, X062T, X062V, X068I, X068L, X068V, X069A, X069G, X069S, X069T, X072I, X072L, X072T, X072V, X073A, X073C, X073S, X076D, X076N, X078A, X078C, X078E, X078H, X078L, X078M, X078N, X078Q, X078S, X078T, X084C, X084G, X084I, X084M, X084V, X086C, X086P, X087A, X087C, X087D, X087E, X087G, X087K, X087L, X087N, X087S, X087V, X088A, X088C, X088G, X088S, X089A, X089D, X089E, X089G, X089H, X089I, X089N, X089Q, X089R, X089S, X089T, X089W, X090C, X090I, X090L, X090M, X090Q, X090T, X090V, X091D, X091F, X091I, X091N, X091S, X091V, X091W, X091Y, X092A, X092G, X092P, X092R, X092V, X093A, X093C, X093G, X093L, X093M, X093T, X093V, X094K, X094R, X095A, X095C, X095I, X095S, X095V, X096F, X096I, X096L, X096M, X097A, X097D, X097E, X097F, X097G, X097H, X097K, X097L, X097M, X097N, X097P, X097Q, X097R, X097S, X097T, X097V, X097W, X097Y, X098A, X098C, X098D, X098E, X098F, X098G, X098K, X098L, X098N, X098P, X098Q, X098R, X098S, X098T, X098V, X098Y, X099A, X099C, X099F, X099G, X099K, X099M, X099P, X099Q, X099R, X099S, X099T, X099V, X099Y, X100D, X100E, X100G, X100I, X100K, X100N, X100R, X100S, X100T, X100V, X100Y, X101A, X101C, X101D, X101E, X101F, X101G, X101H, X101I, X101K, X101N, X101P, X101Q, X101R, X101S, X101T, X101V, X101Y, X102A, X102G, X102T, X103A, X103C, X103D, X103F, X103G, X103I, X103L, X103N, X103P, X103Q, X103R, X103S, X103T, X103V, X103W, X104C, X104F, X104H, X104I, X104L, X104R, X104S, X104T, X104V, X104W, X104Y, X105A, X105C, X105D, X105E, X105F, X105G, X105K, X105L, X105N, X105Q, X105R, X105S, X105T, X105V, X106A, X106D, X106E, X106G, X106I, X106L, X106R, X106S, X106T, X106V, X106W, X107A, X107C, X107F, X107I, X107L, X107M, X107Q, X107S, X107T, X107V, X108A, X108C, X108I, X108S, X108T, X108V, X109A, X109E, X109F, X109G, X109H, X109I, X109K, X109L, X109M, X109N, X109Q, X109R, X109S, X109T, X109W, X109Y, X110A, X110G, X111F, X111I, X111L, X111M, X112D, X112E, X112I, X112Q, X112V, X114A, X114C, X115C, X115E, X115G, X115L, X115M, X115P, X115Q, X115S, X115T, X115W, X115Y, X116A, X116C, X116D, X116F, X116G, X116I, X116K, X116L, X116M, X116N, X116Q, X116S, X116T, X116V, X116W, X117A, X117C, X117N, X117Q, X117R, X117T, X117Y, X118A, X118C, X118D, X118E, X118F, X118G, X118K, X118M, X118N, X118R, X118S, X118V, X118W, X119A, X119F, X119M, X119T, X120A, X120C, X120E, X120F, X120G, X120H, X120I, X120L, X120M, X120N, X120R, X120S, X120T, X120W, X121A, X121C, X121F, X121I, X121L, X121M, X121S, X121T, X121V, X122A, X122G, X122S, X122V, X124G, X124L, X124T, X126A, X126F, X126I, X126L, X126M, X126V, X128A, X128F, X128G, X128I, X128K, X128L, X128M, X128N, X128Q, X128R, X128S, X128T, X128W, X129A, X129E, X129F, X129G, X129M, X129N, X129P, X129R, X129S, X129Y, X130C, X130K, X130L, X130N, X130Q, X130R, X130S, X130V, X130W, X130Y, X131A, X131D, X131E, X131F, X131G, X131K, X131P, X131Q, X132A, X132H, X132I, X132N, X132Q, X132R, X132S, X132T, X133A, X133F, X133K, X133N, X133P, X133S, X133T, X133V, X133Y, X134A, X134S, X134T, X134V, X135L, X135M, X135W, X136E, X136Q, X137A, X137C, X137E, X137H, X137K, X137L, X137M, X137Q, X137R, X137S, X137W, X139C, X139I, X139V, X140D, X140E, X140N, X141D, X141E, X141G, X141H, X141I, X141K, X141L, X141N, X141Q, X141R, X141S, X141V, X141Y, X142A, X142C, X142V, X143C, X143D, X143F, X143H, X143N, X143T, X144A, X144C, X144D, X144G, X144H, X144I, X144L, X144M, X144N, X144R, X144S, X144T, X144V, X144Y, X145A, X145C, X145D, X145F, X145K, X145L, X145N, X145Q, X145R, X146D, X146G, X147I, X147L, X147T, X147V, X148C, X148I, X148L, X148M, X148N, X148V, X149C, X149I, X149L, X149V, X150A, X150L, X150T, X150V, X151A, X151S, X152A, X152S, X156D, X156E, X156L, X156N, X156S, X156T, X158A, X158C, X158E, X158F, X158H, X158K, X158L, X158M, X158Q, X158R, X158S, X158T, X158V, X159A, X159C, X159E, X159G, X159H, X159M, X159P, X159S, X160A, X160C, X160D, X160F, X160I, X160L, X160M, X160N, X160Q, X160S, X160T, X160V, X160Y, X165I, X165L, X165V, X166A, X166C, X166D, X166E, X166H, X166M, X166S, X166T, X167F, X167Y, X170A, X170D, X170E, X170G, X170H, X170K, X170Q, X170R, X170S, X170V, X170Y, X171C, X171Y, X172A, X172C, X172D, X172G, X172I, X172K, X172L, X172M, X172P, X172Q, X172R, X172S, X172V, X172Y, X173A, X173C, X173D, X173H, X173M, X173N, X173Q, X173T, X174A, X174S, X174T, X174V, X175L, X175M, X175V, X176A, X176S, X177C, X177V, X180T, X180V, X182A, X182D, X182E, X182Q, X183A, X183D, X183N, X183Q, X183S, X184D, X184N, X185A, X185C, X185E, X185H, X185K, X185M, X185N, X185Q, X185T, X185V, X186I, X186K, X186L, X186R, X187A, X187C, X188A, X188D, X188E, X188F, X188H, X188I, X188K, X188L, X188P, X188Q, X188S, X188T, X191A, X191D, X191Q, X191S, X194A, X194C, X194D, X194E, X194F, X194H, X194I, X194L, X194M, X194P, X194Q, X194R, X194S, X194T, X194W, X194Y, X195C, X195D, X195E, X195G, X195Q, X198I, X198L, X199C, X199M, X199S, X199V, X203C, X203E, X203T, X203V, X204A, X204C, X204E, X204G, X204N, X204S, X206D, X206E, X206H, X206L, X206N, X206Q, X206S, X209F, X209M, X209W, X209Y, X210A, X210C, X210I, X210L, X210M, X210N, X210P, X211A, X211C, X211E, X211G, X211H, X211I, X211M, X211P, X211Q, X211T, X211V, X212C, X212F, X212G, X212H, X212M, X212N, X212R, X212S, X212Y, X213A, X213D, X213E, X213N, X213Q, X213S, X213T, X215A, X215C, X215D, X215E, X215H, X215I, X215K, X215M, X215N, X215S, X215T, X215V, X215Y, X216A, X216C, X216D, X216E, X216F, X216H, X216I, X216L, X216M, X216N, X216Q, X216S, X216V, X216W, X216Y, X217A, X217C, X217D, X217E, X217F, X217K, X217L, X217M, X217Q, X218C, X218D, X218E, X218N, X218Q, X222C, X222M, X222S, X223A, X223S, X224A, X224N, X224S, X224T, X227A, X227C, X227V, X228A, X228G, X228S, X228V, X230A, X230G, X230N, X230S, X230T, X230V, X231A, X231C, X231F, X231G, X231S, X232A, X232L, X232M, X233A, X233C, X233E, X233G, X233I, X233L, X233N, X233Q, X233S, X233T, X233V, X234L, X234M, X234N, X234Q, X234S, X234T, X234V, X235C, X235F, X235G, X235H, X235I, X235K, X235L, X235M, X235N, X235Q, X235R, X235S, X235V, X235W, X235Y, X236A, X236C, X236E, X236F, X236H, X236N, X236Q, X236S, X236T, X236Y, X237A, X237C, X237F, X237G, X237H, X237I, X237K, X237L, X237M, X237Q, X237R, X237S, X237T, X237V, X237W, X237Y, X238C, X238D, X238E, X238F, X238G, X238H, X238I, X238K, X238L, X238M, X238N, X238Q, X238R, X238S, X238T, X238V, X238Y, X239C, X239D, X239F, X239G, X239H, X239K, X239L, X239M, X239N, X239P, X239Q, X239R, X239S, X239T, X239V, X239W, X239Y, X240A, X240C, X240E, X240F, X240I, X240K, X240M, X240N, X240Q, X240R, X240S, X240T, X240W, X240Y, X241A, X241C, X241D, X241E, X241F, X241G, X241H, X241I, X241K, X241L, X241M, X241N, X241Q, X241R, X241S, X241T, X241V, X241W, X241Y, X242A, X242C, X242D, X242G, X242H, X242I, X242L, X242M, X242P, X242Q, X242S, X242T, X242V, X243C, X243D, X243E, X243F, X243G, X243H, X243I, X243L, X243M, X243N, X243P, X243Q, X243S, X243T, X243V, X243W, X244D, X244E, X244F, X244H, X244I, X244L, X244M, X244N, X244Q, X244S, X244T, X244V, X244W, X245A, X245C, X245E, X245G, X245H, X245K, X245L, X245P, X245Q, X245S, X245V, X245W, X245Y, X246A, X246C, X246I, X246L, X246M, X246T, X246V, X247A, X247C, X247F, X247G, X247H, X247K, X247L, X247M, X247N, X247R, X247S, X247T, X247V, X247W, X247Y, X248C, X248D, X248E, X248G, X248H, X248I, X248L, X248N, X248P, X248R, X248S, X248T, X248V, X248Y, X249A, X249D, X249E, X249F, X249G, X249H, X249I, X249K, X249L, X249M, X249N, X249Q, X249S, X249T, X249W, X249Y, X250C, X250I, X250L, X250M, X250V, X251A, X251D, X251E, X251F, X251G, X251K, X251L, X251M, X251Q, X251R, X251T, X251V, X251Y, X252A, X252C, X252D, X252F, X252G, X252H, X252I, X252K, X252L, X252M, X252N, X252R, X252S, X252V, X252W, X253A, X253E, X253H, X253M, X253S, X253T, X253W, X255A, X255C, X255D, X255E, X255I, X255L, X255N, X255Q, X255T, X255V, X255Y, X256A, X256C, X256D, X256E, X256G, X256H, X256I, X256K, X256L, X256M, X256N, X256P, X256S, X256V, X256W, X256Y, X258D, X258G, X259A, X259C, X259E, X259P, X259Q, X259S, X260A, X260D, X260E, X260H, X260I, X260N, X260P, X260S, X260T, X260V, X261A, X261C, X261E, X261F, X261I, X261K, X261L, X261N, X261P, X261Q, X261S, X261T, X261V, X261W, X261Y, X262A, X262C, X262D, X262F, X262H, X262I, X262L, X262M, X262Q, X262T, X262V, X262Y, X265A, X265D, X265S, X267I, X267L, X268A, X268L, X268V, X269D, X269H, X269N, X269Q, X269S, X270A, X270C, X270G, X270I, X270L, X270N, X270S, X270T, X270V, X271C, X271E, X272A, X272C, X272D, X272E, X272F, X272G, X272H, X272L, X272M, X272N, X272P, X272S, X272T, X272W, X272Y, X273A, X273C, X273G, X273S, X274A, X274C, X274G, X274L, X274M, X274N, X274S, X274T, X275D, X275E, X275F, X275H, X275K, X275L, X275M, X275P, X275Q и X275R, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
9. Вариант субтилизина по п.1, где указанный вариант субтилизина содержит дополнительную замену в одном или более положениях, выбранных из группы, состоящей из: 18, 52, 72, 117, 119, 127, 144, 185, 209 и 213, и где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
10. Выделенный вариант субтилизина по любому из пп.1-9, где указанный вариант субтилизина представляет собой вариант GG36.
11. Выделенный вариант субтилизина по п.1, где указанный выделенный вариант субтилизина содержит комбинацию замен, выбранную из:
S87N/Q109Q/G118D/S128L/P129Q/S130A/S188S/T213E/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213R/N248D,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213T/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213E/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213T/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213T/N248R,
S87R/Q109R/G118V/S128L/P129Q/S130A/S188D/T213E/N248R,
S87R/Q109Q/G118R/S128L/P129Q/S130A/S188D/T213T/N248R,
S87R/Q109R/G118R/S128L/P129Q/S130A/S188D/T213T/N248R,
S87N/Q109Q/G118R/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213R/N248R,
S87R/Q109Q/G118R/S128L/P129Q/S130A/S188S/T213E/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213T/N248N,
S87R/Q109Q/G118R/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213E/N248R,
S87D/Q109D/G118D/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213R/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213T/N248R,
S87R/Q109R/G118R/S128L/P129Q/S130A/S188D/T213E/N248R,
S87R/Q109Q/G118R/S128L/P129Q/S130A/S188D/T213E/N248N,
S87N/Q109R/G118R/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213E/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213E/N248R,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213T/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213R/N248D,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213T/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213R/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213T/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213T/N248D,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213R/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213E/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213T/N248D,
S87R/Q109D/G118D/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213R/N248R,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213R/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213R/N248N,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213E/N248N,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213E/N248N,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213R/N248R,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213T/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213R/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213T/N248N,
S87R/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109D/G118V/S128L/P129Q/S130A/S188R/T213R/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213T/N248D,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213E/N248D,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213R/N248D,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213E/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213E/N248D,
S87R/Q109D/G118R/S128L/P129Q/S130A/S188D/T213E/N248N,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213R/N248R,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213R/N248N,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213T/N248R,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213E/N248R,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213R/N248D и
S87N/Q109Q/G118V/S128LP129Q/S130A/S188R/T213E/N248R, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
12. Выделенный вариант субтилизина по п.1, где указанный вариант содержит комбинацию замен, выбранную из: S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213T/N248D,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213R/N248N,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213R/N248D,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213E/N248D,
S87R/Q109D/G118R/S128L/P129Q/S130A/S188S/T213E/N248R,
S87R/Q109D/G118R/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109D/G118V/S128L/P129Q/S130A/S188D/T213E/N248D и
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213E/N248D, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
13. Выделенный вариант субтилизина по п.1, где указанный вариант содержит комбинацию замен, выбранную из: S87D/Q109D/G118R/S128L/P129Q/S130A/S188R/T213E/N248D,
S87N/Q109D/G118R/S128L/P129Q/S130A/S188D/T213E/N248R,
S87D/Q109Q/G118D/S128L/P129Q/S130A/S188S/T213E/N248D,
S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213E/N248D,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213E/N248D,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213T/N248D,
S87R/Q109D/G118V/S128L/P129Q/S130A/S188D/T213E/N248R,
S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213R/N248D и
S87R/Q109D/G118R/S128L/P129Q/S130A/S188D/T213T/N248R, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
14. Выделенный вариант субтилизина по п.1, где указанный вариант содержит комбинацию замен, выбранную из: A001C/S024V/I107L, A001I/R045T/I107R/A172T, A001K/A172L, A001K/A172W, A001K/I072C/L126F, A001K/I072C/L126F/S164Q, A001K/I072C/V104I/L126F, A001K/I072C/V104I/L126F/S164Q, A001K/I072V, A001K/I072V/L126F, A001K/I072V/L126F/S164F, A001K/I072V/V104I, A001K/I072V/V104I/L126F/S164I, A001K/I072V/V104I/S164F, A001K/I072V/V104I/S164Q, A001K/I107T/A172Q, A001K/I107V/G127Q, A001K/I107V/G127Q/A172Q, A001K/I107V/G127R/A172Q, A001K/L126F, A001K/L126F/S164F, A001K/L126F/S164I, A001K/L126F/S164Q, A001K/R045F/I072C, A001K/R045F/I072C/L126F, A001K/R045F/I072C/V104I/S164F, A001K/R045F/I072V/L126F/S164I, A001K/R045F/I072V/L126F/S164Q, A001K/R045F/I072V/S164F, A001K/R045F/I072V/V104G/S164T, A001K/R045F/I107L/A172N, A001K/R045F/I107T, A001K/R045F/I107V/G127Q, A001K/R045F/L126F/S164F, A001K/R045F/L126F/S164I, A001K/R045F/S164F, A001K/R045F/V104I/L126F/S164Q, A001K/R045K/I072C/L126F/S164Q, A001K/R045K/I072V/L126F, A001K/R045K/I072V/L126F/S164F, A001K/R045K/I072V/L126F/S164Q, A001K/R045K/I072V/S164F, A001K/R045K/I072V/S164Q, A001K/R045K/I072V/V104I/L126F/S164F, A001K/R045K/I072V/V104I/L126F/S164I, A001K/R045K/I072V/V104I/L126F/S164Q, A001K/R045K/I107A/A172T, A001K/R045K/I107R/G127A/A172N, A001K/R045K/I107T/G127R, A001K/R045K/I107T/G127R/A172Q, A001K/R045K/L126F, A001K/R045K/L126F/S164I, A001K/R045K/L126F/S164P, A001K/R045K/L126F/S164Q, A001K/R045K/S164F, A001K/R045K/V104I/S164Q, A001K/R045K/V104L/S164A, A001K/R045N/I107T/G127Q, A001K/R045S/V104C/L126Y/S164F, A001K/S024H/I107T/G127Q, A001K/S024H/I107T/G127R, A001K/S024H/I107V, A001K/S024H/R045N/I107T, A001K/S024H/R045N/I107T/G127Q/A172Q, A001K/S024L/R045A, A001K/S024N/I107V/G127T, A001K/S024N/R045K/A172Y, A001K/S024R/I107T, A001K/S024R/R045N/A172C, A001K/S024R/R045N/I107T, A001K/S024R/R045N/I107T/G127R, A001K/S024T/I107T/G127Q/A172Q, A001K/S024T/R045N/G127Q/A172Q, A001K/S024W/I107T/G127R, A001K/S024W/I107V/G127T/A172Q, A001K/S164Q, A001K/V104E, A001K/V104I/L126F, A001K/V104I/L126F/S164I, A001K/V104I/L126F/S164Q, A001L/I072C, A001L/R045Q/G127H/A172R, A001L/S024D/R045Y/I107Y, A001M/R045S/I107E/A172D, A001P/A172V, A001Q/R045K/I107T, A001R/A172G, A001R/A172Q, A001R/I107T, A001R/I107T/A172Q, A001R/I107T/G127Q/A172Q, A001R/I107V/A172Q, A001R/R045F, A001R/R045K/A172Q, A001R/R045K/G127R, A001R/R045K/I107T/G127Q, A001R/R045K/I107V/G127R, A001R/R045N/A172K, A001R/R045N/A172Q, A001R/R045N/I107T, A001R/R045N/I107T/G127Q, A001R/R045N/I107V/A172V, A001R/R045N/I107V/G127Q/A172Q, A001R/R045N/I107V/G127R, A001R/S024H/I107T, A001R/S024H/R045K/I107T, A001R/S024H/R045K/I107T/A172Q, A001R/S024H/R045N/I107T/A172Q, A001R/S024N, A001R/S024R, A001R/S024R/A172H, A001R/S024R/A172Q, A001R/S024R/I107V/A172L, A001R/S024R/I107V/G127R, A001R/S024R/R045F/I107T/G127Q, A001R/S024R/R045F/I107T/G127R, A001R/S024R/R045K/I107T/G127Q, A001R/S024T/I107T/A172Q, A001R/S024T/R045K/A172Q, A001R/S024T/R045K/I107L/G127P/A172P, A001R/S024T/R045N/I107T/A172Q, A001R/S024W/I107V/A172S, A001R/S024W/R045F/I107V/G127Q, A001R/S024W/R045F/I107V/G127R, A001R/S024W/R045K, A001R/S024W/R045K/I107T/A172Q, A001R/S024W/R045N/I107T, A001S/A172V, A001S/S024L/I107Y/A172S, A001T/S024H/I107L/A172Y, A001V/I107L/A172I, G097P/N185Q/A215I, G097P/Y209W/A215V/S256W, G118L/G127R/S132L, G118T/G127Q, I107A/G127K/A172G, I107C/A172S, I107P/A172S, I107Q/A172D, L126A/S164N, L126F/S164V, M119F/N185R, M119F/N185R/Y209W/S256T, M119F/N185V/Y209W, M119F/S144T/N185V/S256I, M119F/S144W/N185R/S256L, M119F/Y209W, M119F/Y209W/S256I, N018K/A215I, N018K/A215R, N018K/A215V, N018K/G097P/A215I/S256W, N018K/G097P/N185K/S256W, N018K/G097P/N185K/Y209W/A215I/S256W, N018K/G097P/N185Q/Y209W/A215V/S256W, N018K/G097P/N185R/A215R/S256W, N018K/G097P/N185R/A215V/S256W, N018K/G097P/N185R/S256W, N018K/G097P/N185R/Y209W, N018K/G097P/N185R/Y209W/S256W, N018K/G097P/Y209W/A215V/S256W, N018K/N185K, N018K/N185K/A215V/S256W, N018K/N185K/S256W, N018K/N185K/Y209W/A215R, N018K/N185Q/A215R/S256W, N018K/N185Q/A215V/S256W, N018K/N185Q/S256W, N018K/N185Q/Y209W/S256W, N018K/N185R/A215I/S256W, N018K/N185R/A215R/S256W, N018K/N185R/A215V, N018K/N185R/Y209W/A215V/S256W, N018K/S256W, N018Q/G097P/N185K/A215V, N018Q/G097P/N185K/Y209W, N018Q/N185K, N018Q/N185K/S256W, N018Q/N185K/Y209W/A215I/S256W, N018Q/N185K/Y209W/A215V, N018Q/N185K/Y209W/A215V/S256W, N018Q/N185Q/A215V, N018Q/N185Q/S256W, N018Q/N185Q/Y209W/S256W, N018Q/N185R/A215R, N018Q/N185R/S256W, N018R/G097P/N185Q/A215I, N018R/G097P/Y209W/A215V, N018R/G097P/Y209W/S256W, N018R/N185K/A215V/S256W, N018R/N185K/S256W, N018R/N185Q/A215V/S256W, N018R/N185Q/Y209W/S256W, N018R/N185R/A215R, N018R/N185R/S256W, N018R/N185R/Y209W/S256W, N018R/Y209W/A215V/S256W, N018R/Y209W/S256W, N043E/S101E, N043E/S101E/N248K, N043E/S101F, N043E/S101N/N117Y, N043E/S101V, N043E/S101Y/N117I/N248K, N043E/S101Y/N117Y/N248K, N043F/N117I, N043F/S101E/N117I/N248K, N043F/S101R, N043I/N117Y, N043I/S101E/N248I, N043I/S101F/N117Y/N248K, N043I/S101N/N117Y/N248K, N043I/S101R/N248K, N043I/S101Y/N117I/N248I, N043I/S101Y/N117Y/N248K, N043S/N117Y/N248K, N043S/N248I, N043S/S101E, N043S/S101E/N117I/N248I, N043S/S101E/N117Y/N248K, N043S/S101E/N248K, N043S/S101F, N043S/S101F/N248K, N043S/S101N/N117I, N043S/S101R/N117I/N248K, N043S/S101V/N117I/N248I, N043S/S101Y, N043S/S101Y/N117I, N043S/S101Y/N117Y, N043S/S101Y/N248K, N043V/N117Y/N248I, N043V/N248I, N043V/N248K, N043V/S101E/N117I/N248K, N043V/S101E/N248I, N043V/S101F, N043V/S101F/N117I, N043V/S101F/N117Y, N043V/S101F/N248K, N043V/S101R/N117I/N248K, N043V/S101T/N248K, N043V/S101V/N117I, N043V/S101V/N248K, N043V/S101Y, N043V/S101Y/N117I, N043V/S101Y/N117I/N248K, N185K/A215V, N185K/Y209W/S256W, N185Q/A215R/S256W, N185Q/S256W, N185R/S256W, N185R/Y209W/A215I, N185R/Y209W/S256W, N185V/Y209W, P052I/M119F, P052I/M119F/S144T/N185R/Y209W, P052I/M119F/S144V/Y209W, P052I/M119F/S144Y/N185R, P052I/M119F/S144Y/N185R/Y209W/S256I, P052I/M119F/Y209W/S256I, P052I/N185R/Y209W/S256I, P052I/N185V/Y209W, P052I/N185V/Y209W/S256I, P052I/S144T, P052I/S144T/N185R/Y209W/S256T, P052I/S144T/N185V/Y209W/S256T, P052I/S144T/S256I, P052I/S144T/Y209W/S256I, P052I/S144T/Y209W/S256T, P052I/S144V/Y209W, P052I/S144Y/N185R/Y209W, P052I/S144Y/N185V, P052I/S144Y/N185V/S256T, P052I/S144Y/S256T, P052I/S144Y/Y209W/S256I, P052V/M119F/N185V/Y209W/S256I, P052V/M119F/S144T, P052V/M119F/S144T/N185R/S256T, P052V/M119F/S144T/N185R/Y209W, P052V/M119F/S144T/S256T, P052V/M119F/S144T/Y209W, P052V/M119F/S144V/N185V/S256I, P052V/N185V/Y209W/S256I, P052V/S144T, P052V/S144T/N185V/Y209W/S256I, P052V/S144T/N185V/Y209W/S256T, P052V/S144T/Y209W/S256I, P052V/S144V/N185R/Y209W/S256T, P052V/S144V/N185V/Y209W, P052V/S144V/Y209W/S256I, P052V/S144W/N185R, P052V/S144W/N185V/Y209W, P052V/S144W/Y209W, P052V/S144Y/S256T, P052V/S144Y/Y209W, P052V/S256T, Q109A/G118S, Q109D/G118V/G127H, Q109H/G118S/S132P, Q109H/G127R, Q109I/G118R, Q109I/G118S, Q109I/G127C, Q109I/G127T/S132Y, Q109I/S132N, Q109K/G118F/G127Q/S132N, Q109L/G118L/G127Q/S132N, Q109P/G118Q, Q109R/G118V, Q109S/G118R, Q109T/G118N, Q109V/G118F, Q109V/G127Q, Q109V/G127R, Q109V/G127R/S132N, Q109V/G127R/S132Y, R045F/I072V/L126F/S164Q, R045F/I107T, R045K/I072C/V104I/L126F/S164F, R045K/I107T, R045K/L126F/S164F, R045L/I072S/S164I, R045N/I107A/A172R, R045N/I107T/A172Q, R045N/I107V/A172Q, R045S/I107L, R045V/I107R/A172D, S024A/G118I, S024A/Q109Y/G118C, S024C/Q109K/G118I, S024G/G118F/G127R/S132N, S024H/G118K/G127I, S024H/G118L/G127T/S132Y, S024H/G127R, S024H/I107K, S024H/Q109L, S024H/Q109T/G118R, S024H/Q109V/G127R/S132N, S024H/Q109V/G127T, S024H/S099K/G118F/G127R, S024H/S099K/G118R/G127R/S132H, S024H/S099K/S103P, S024H/S099K/S103P/G118F, S024H/S099K/S103R/G118A/S132P, S024H/S099Q/G118R/G127Q/S132H, S024H/S099Q/G118R/S132H, S024H/S099Q/S103N/G118R/G127T/S132N, S024H/S099Q/S103N/G118T/S132H, S024H/S099Q/S103P, S024H/S099Q/S103P/G118R/S132H, S024H/S099Q/S103P/S132H, S024H/S099Q/S132H, S024H/S099Q/S132N, S024H/S099T/G118R, S024H/S099T/S103N, S024H/S099T/S103N/G127R/S132N, S024H/S099T/S103N/S132N, S024H/S099T/S103P/G118R, S024H/S099T/S103P/G118R/S132H, S024H/S099T/S103P/S132N, S024H/S103N/S132H, S024H/S103P/G127R/S132R, S024H/S132N, S024H/S132R, S024I/I107R/G127I/A172R, S024L/G127R, S024L/Q109K/G127R/S132Y, S024L/Q109T/G118V/G127Q/S132R, S024L/Q109V/G118R/G127Q, S024M/G118V, S024N/G118R/G127L/S132Y, S024N/G127R, S024N/I107T, S024N/Q109T/G127R/S132N, S024N/Q109T/G127T/S132R, S024N/Q109T/G127T/S132Y, S024N/Q109V/G118L/G127Q, S024N/Q109V/G118R/G127T/S132Y, S024N/Q109V/G127R/S132N, S024N/R045K/A172L, S024N/R045K/I107V/A172N, S024N/S099T/G127T/S132H, S024N/S132R, S024P/S099Q/S103P/G118R, S024Q/Q109R/S132R, S024R/G118H/S132V, S024R/G118L/G127R/S132R, S024R/G118R/S132N, S024R/Q109C/G127H, S024R/Q109I/G127T, S024R/Q109K/G118T/G127Q/S132N, S024R/Q109L/G127Q, S024R/Q109R/G118T/G127R, S024R/Q109T/G127R/S132N, S024R/Q109T/G127R/S132Y, S024R/Q109T/G127T/S132Y, S024R/Q109T/S132Y, S024R/Q109V, S024R/Q109V/G118C/S132A, 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S087N/G118V/S128L/P129Q/S130A/N043V/S101T, S087N/G118V/S128L/P129Q/S130A/N043V/S101T/N248K, S087N/G118V/S128L/P129Q/S130A/N043V/S101Y, S087N/G118V/S128L/P129Q/S130A/N043V/S101Y/N248I, S087N/G118V/S128L/P129Q/S130A/N043V/S144T/N185V/S256I, S087N/G118V/S128L/P129Q/S130A/N043V/S144W/N185V, S087N/G118V/S128L/P129Q/S130A/N043V/S144W/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/N043V/S144Y/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/N043V/S144Y/N185V/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/N043W/P052V/S101E/N248K, S087N/G118V/S128L/P129Q/S130A/N043W/P052V/S101Y, S087N/G118V/S128L/P129Q/S130A/N043W/P052V/S101Y/N248K, S087N/G118V/S128L/P129Q/S130A/N043W/S101E, S087N/G118V/S128L/P129Q/S130A/N043W/S101F/N248K, S087N/G118V/S128L/P129Q/S130A/N185K/A215V/S256W, S087N/G118V/S128L/P129Q/S130A/N185K/Y209W/A215V, S087N/G118V/S128L/P129Q/S130A/N185K/Y209W/A215V/S256W, S087N/G118V/S128L/P129Q/S130A/N185Q/Y209W/A215R, S087N/G118V/S128L/P129Q/S130A/N185Q/Y209W/A215V/S256W, S087N/G118V/S128L/P129Q/S130A/N185R/A215V/S256W, S087N/G118V/S128L/P129Q/S130A/N185R/S256T, S087N/G118V/S128L/P129Q/S130A/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/N185R/Y209W/A215V/S256R, S087N/G118V/S128L/P129Q/S130A/N185R/Y209W/S256L, S087N/G118V/S128L/P129Q/S130A/N185V/Y209W, S087N/G118V/S128L/P129Q/S130A/N248I, S087N/G118V/S128L/P129Q/S130A/N248K, S087N/G118V/S128L/P129Q/S130A/P052I/N248K, S087N/G118V/S128L/P129Q/S130A/P052I/S101E/N248K, S087N/G118V/S128L/P129Q/S130A/P052I/S101F/N248K, S087N/G118V/S128L/P129Q/S130A/P052I/S101V/N248K, S087N/G118V/S128L/P129Q/S130A/P052V, S087N/G118V/S128L/P129Q/S130A/P052V/G097T/N185R/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S101E/N248I, S087N/G118V/S128L/P129Q/S130A/P052V/S101R, S087N/G118V/S128L/P129Q/S130A/P052V/S144T, S087N/G118V/S128L/P129Q/S130A/P052V/S144T/N185R/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S144T/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/P052V/S144T/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/N185R/S256I, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/N185R/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/N185V/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/Y209W, S087N/G118V/S128L/P129Q/S130A/P052V/S144Y/N185R/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/P052V/S144Y/N185R/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S144Y/N185V, S087N/G118V/S128L/P129Q/S130A/P052V/S144Y/N185V/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/P052V/Y209W, S087N/G118V/S128L/P129Q/S130A/P052V/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/Q109I, S087N/G118V/S128L/P129Q/S130A/Q109L/G127Q, S087N/G118V/S128L/P129Q/S130A/Q109T, S087N/G118V/S128L/P129Q/S130A/Q109T/G127Q, S087N/G118V/S128L/P129Q/S130A/Q109V, S087N/G118V/S128L/P129Q/S130A/R045F/I107T/A172Q, S087N/G118V/S128L/P129Q/S130A/R045K/I072C/L126F, S087N/G118V/S128L/P129Q/S130A/R045N/A172Q, S087N/G118V/S128L/P129Q/S130A/R045N/I107V/A172P, S087N/G118V/S128L/P129Q/S130A/R045S, S087N/G118V/S128L/P129Q/S130A/S024H, S087N/G118V/S128L/P129Q/S130A/S024H/I107V/A172S, S087N/G118V/S128L/P129Q/S130A/S024H/Q109V, S087N/G118V/S128L/P129Q/S130A/S024H/Q109V/G127Q, S087N/G118V/S128L/P129Q/S130A/S024H/R045N/I107T, S087N/G118V/S128L/P129Q/S130A/S024H/S099K/S103N, S087N/G118V/S128L/P129Q/S130A/S024H/S099K/S103N/S132N, S087N/G118V/S128L/P129Q/S130A/S024H/S099Q, S087N/G118V/S128L/P129Q/S130A/S024H/S099Q/S103P, S087N/G118V/S128L/P129Q/S130A/S024H/S099Q/S132N, S087N/G118V/S128L/P129Q/S130A/S024H/S099T/S103P, S087N/G118V/S128L/P129Q/S130A/S024H/S099T/S103P/S132H, S087N/G118V/S128L/P129Q/S130A/S024H/S099T/S103P/S132N, S087N/G118V/S128L/P129Q/S130A/S024H/S099T/S132N, S087N/G118V/S128L/P129Q/S130A/S024H/S103P/S132N, S087N/G118V/S128L/P129Q/S130A/S024L/S099K/S103P/S132N, S087N/G118V/S128L/P129Q/S130A/S024N/I107N, S087N/G118V/S128L/P129Q/S130A/S024N/I107T, S087N/G118V/S128L/P129Q/S130A/S024N/I107V, S087N/G118V/S128L/P129Q/S130A/S024N/Q109K, S087N/G118V/S128L/P129Q/S130A/S024N/Q109T/G127T, S087N/G118V/S128L/P129Q/S130A/S024R, S087N/G118V/S128L/P129Q/S130A/S024R/G127R, S087N/G118V/S128L/P129Q/S130A/S024R/I107T/A172Q, S087N/G118V/S128L/P129Q/S130A/S024R/I107V/A172S, S087N/G118V/S128L/P129Q/S130A/S024R/Q109K, S087N/G118V/S128L/P129Q/S130A/S024R/Q109L, S087N/G118V/S128L/P129Q/S130A/S024R/Q109L/G127Q, S087N/G118V/S128L/P129Q/S130A/S024R/Q109V, S087N/G118V/S128L/P129Q/S130A/S024R/R045F/I107T, S087N/G118V/S128L/P129Q/S130A/S024T, S087N/G118V/S128L/P129Q/S130A/S024T/G127T, S087N/G118V/S128L/P129Q/S130A/S024T/I107T/A172Q, S087N/G118V/S128L/P129Q/S130A/S024T/Q109V/G127T, S087N/G118V/S128L/P129Q/S130A/S024T/R045N/I107V/A172S, S087N/G118V/S128L/P129Q/S130A/S024W, S087N/G118V/S128L/P129Q/S130A/S024W/G127T, S087N/G118V/S128L/P129Q/S130A/S024W/I107T, S087N/G118V/S128L/P129Q/S130A/S024W/Q109I, S087N/G118V/S128L/P129Q/S130A/S024W/Q109L, S087N/G118V/S128L/P129Q/S130A/S024W/Q109R/G127Q, S087N/G118V/S128L/P129Q/S130A/S024W/Q109T, S087N/G118V/S128L/P129Q/S130A/S024W/Q109V, S087N/G118V/S128L/P129Q/S130A/S024W/R045F/I107V/A172Q, S087N/G118V/S128L/P129Q/S130A/S024W/S099K, S087N/G118V/S128L/P129Q/S130A/S024W/S099K/S103P, S087N/G118V/S128L/P129Q/S130A/S024W/S099K/S132H, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S103N/G127R/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S103N/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S103P, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S132H, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099T, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/G127R/S132H, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S103N, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S103N/G127R/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S103N/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S103P/S132H, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S132N, S087N/G118V/S128L/P129Q/S130A/S099K, S087N/G118V/S128L/P129Q/S130A/S099K/S103N, S087N/G118V/S128L/P129Q/S130A/S099K/S103P, S087N/G118V/S128L/P129Q/S130A/S099K/S132H, S087N/G118V/S128L/P129Q/S130A/S099Q, S087N/G118V/S128L/P129Q/S130A/S099Q/S103N, S087N/G118V/S128L/P129Q/S130A/S099Q/S103P, S087N/G118V/S128L/P129Q/S130A/S099Q/S103P/S132H, S087N/G118V/S128L/P129Q/S130A/S099Q/S132H, S087N/G118V/S128L/P129Q/S130A/S099T/G127Q, S087N/G118V/S128L/P129Q/S130A/S099T/S103N, S087N/G118V/S128L/P129Q/S130A/S099T/S103N/S132H, S087N/G118V/S128L/P129Q/S130A/S099T/S103P, S087N/G118V/S128L/P129Q/S130A/S099T/S103P/S132H, S087N/G118V/S128L/P129Q/S130A/S099T/S132H, S087N/G118V/S128L/P129Q/S130A/S101E, S087N/G118V/S128L/P129Q/S130A/S101E/N248K, S087N/G118V/S128L/P129Q/S130A/S101F/N248K, S087N/G118V/S128L/P129Q/S130A/S101N/N248K, S087N/G118V/S128L/P129Q/S130A/S101R/N248K, S087N/G118V/S128L/P129Q/S130A/S101Y, S087N/G118V/S128L/P129Q/S130A/S103N/S132H, S087N/G118V/S128L/P129Q/S130A/S103P, S087N/G118V/S128L/P129Q/S130A/S144T/N185R, S087N/G118V/S128L/P129Q/S130A/S144T/N185R/S256I, S087N/G118V/S128L/P129Q/S130A/S144T/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/S144T/N185R/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/S144T/N185V/S256I, S087N/G118V/S128L/P129Q/S130A/S144T/N185V/Y209W, S087N/G118V/S128L/P129Q/S130A/S144T/S256I, S087N/G118V/S128L/P129Q/S130A/S144T/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/S144V, S087N/G118V/S128L/P129Q/S130A/S144V/N185R/S256I, S087N/G118V/S128L/P129Q/S130A/S144V/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/S144V/N185V, S087N/G118V/S128L/P129Q/S130A/S144V/N185V/S256I, S087N/G118V/S128L/P129Q/S130A/S144V/N185V/Y209W, S087N/G118V/S128L/P129Q/S130A/S144V/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/S144V/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/S144W/Y209W, S087N/G118V/S128L/P129Q/S130A/S144Y/N185R, S087N/G118V/S128L/P129Q/S130A/S144Y/N185R/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/S144Y/N185V/Y209W, S087N/G118V/S128L/P129Q/S130A/S164I, S087N/G118V/S128L/P129Q/S130A/S164Q, S087N/G118V/S128L/P129Q/S130A/V104I, S087N/G118V/S128L/P129Q/S130A/V104I/L126F/S164F, S087N/G118V/S128L/P129Q/S130A/V104I/L126F/S164I, S087N/G118V/S128L/P129Q/S130A/V104I/S164I, S087N/G118V/S128L/P129Q/S130A/V104I/S164Q, S087N/G118V/S128L/P129Q/S130A/Y209W, S087N/G118V/S128L/P129Q/S130A/Y209W/A215I/S256W, S087N/G118V/S128L/P129Q/S130A/Y209W/S256W, S099H/S103I/G118N, S099K/G118R, S099K/S103N/G118T, S099K/S103P/G118L/S132N, S099K/S103P/G127Q, S099K/S103P/S132H, S099K/S103R/G118A/S132P, S099N/S132R, S099Q/G118F/G127R, S099Q/G118T/G127Q, S099Q/S103N/G118R/S132N, S099Q/S103N/G118T, S099Q/S103N/G118T/S132H, S099Q/S103N/S132H, S099Q/S103P/G118R, S099Q/S103P/G118R/S132N, S099Q/S103P/S132H, S099Q/S132R, S099T/G118F, S099T/G118R/S132H, S099T/G118T/S132H, S099T/G118T/S132N, S099T/S103P, S099T/S103P/G118L/S132H, S101E/N117I, S101E/N117Y/N248K, S101F/N117I/N248I, S101F/N117Y/N248I, S101F/N117Y/N248K, S101F/N248K, S101R/N117I/N248K, S101R/N117Y, S101R/N248K, S101T/N117Y/N248K, S101T/N248I, S101V/N248K, S101Y/N117I/N248K, S101Y/N117Y, S101Y/N248K, S103G/G118I/G127E/S132E, S103L/S132E, S103R/S132P, S144T/N185R, S144T/N185R/Y209W/S256I, S144T/N185R/Y209W/S256T, S144T/N185V, S144V/N185R/S256I, S144V/N185R/Y209W, S144V/N185R/Y209W/S256T, S144V/S256I, S144V/Y209W, S144W/N185R/Y209W/S256T, S144W/N185V/Y209W/S256I, S144W/S256I, S144Y/Y209W/S256I, V104E/L126I/S164L, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
15. Выделенный вариант субтилизина по п.1, где указанный вариант содержит комбинацию замен, выбранную из: TS87N/G118V/S128L/P129Q/S130A/S24W/S101L/Q109K, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101L/Q109R/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/I72V/Q109R/S164I, S87N/G118V/S128L/P129Q/S130A/S101Y/Q109R/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101Y/Q109L/N185R/A215R, S87R/G118R/S128L/P129Q/S130A/S101K/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/S24W/S101L/Q109L, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101Y/Q109R/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/I72V/S101L/Q109R/S164I, S87N/G118V/S128L/P129Q/S130A/S101Y/Q109R/S188D/T213E/N248R, S87R/G118R/S128L/P129Q/S130A/I72V/S164I/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N76D, S87N/G118V/S128L/P129Q/S130A/S24W/S101Y/Q109K, S87N/G118V/S128L/P129Q/S130A/N43S/P52V/S101R/Q109R, S87N/G118V/S128L/P129Q/S130A/I72V/S101Y/Q109R/S164I, S87N/G118V/S128L/P129Q/S130A/S101L/Q109L/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/I72V/Q109R/S164I/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N76D/S101L, S87N/G118V/S128L/P129Q/S130A/S24W/S101Y/Q109L, S87N/G118V/S128L/P129Q/S130A/N76D/S164I, S87R/G118R/S128L/P129Q/S130A/N76D/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/S101Y/Q109L/S188D/N248R, S87R/G118R/S128L/P129Q/S130A/V118R/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N76D/S101M, S87N/G118V/S128L/P129Q/S130A/N18K/N76D/G97P/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/I72V/S101L/S164I, S87R/G118R/S128L/P129Q/S130A/S101L/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/S101Y/Q109L/S188D/T213E/N248R, S87R/G118R/S128L/P129Q/S130A/Q109L/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101L/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/I72V/S101Y/S164I, S87N/G118V/S128L/P129Q/S130A/S101Y/T213E/N248R, S87N/G118V/S128L/P129Q/S130A/I72V/S101L/Q109L/S164I, S87R/G118R/S128L/P129Q/S130A/S101V/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101Y/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/I72V/S101V/S164I, S87N/G118V/S128L/P129Q/S130A/S101L/T213E/N248R, S87N/G118V/S128L/P129Q/S130A/I72V/S101Y/Q109L/S164I, S87R/G118R/S128L/P129Q/S130A/S101H/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/Q109R/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/I72V/S101H/S164I, S87N/G118V/S128L/P129Q/S130A/S101L/Q109R/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101L/Q109L/N185R/A215R, S87R/G118R/S128L/P129Q/S130A/S101M/S188D/N248R, N76D/S87R/G118R/S128L/P129Q/S130A/S188D, N76D/S87R/G118R/S128L/P129Q/S130A/S188D/N248K, S87N/G118V/G127S/S128L/P129Q/S130A, S87N/S101H/G118V/S128L/P129Q/S130A, S87N/S101K/G118V/S128L/P129Q/S130A, S87N/S101V/G118V/S128L/P129Q/S130A, S87N/S101Y/G118V/S128L/P129Q/S130A, S87N/S101L/G118V/S128L/P129Q/S130A, I72V/S87N/G118V/S128L/P129Q/S130A//S164I, где положения пронумерованы в соответствии с аминокислотной последовательностью субтилизина BPN' B.amyloliquefaciens, указанной в качестве SEQ ID NO: 1.
16. Выделенная нуклеиновая кислота, кодирующая вариант субтилизина, указанный в любом из пп.1-15.
17. Экспрессирующий вектор, содержащий нуклеиновую кислоту по п.16.
18. Клетка-хозяин, содержащая экспрессирующий вектор по п.17.
19. Моющая композиция, содержащая вариант субтилизина, указанный в любом из пп.1-15.
20. Моющая композиция по п.19, где указанная композиция содержит жидкий, гелеобразный, таблетированный, порошковый и/или гранулярный детергент.
21. Моющая композиция по п.19 или 20, где указанная моющая композиция выбрана из детергентов для стирки и детергентов для мытья посуды.
22. Моющая композиция по п.21, содержащая детергент для стирки.
23. Моющая композиция по п.22, где указанный детергент для стирки представляет собой высокоэффективный детергент.
24. Моющая композиция по п.21, содержащая детергент для мытья посуды, выбранный из детергентов для ручного и автоматического мытья посуды.
25. Моющая композиция по п.19, дополнительно содержащая по меньшей мере один отбеливатель.
26. Моющая композиция по п.19, где указанная моющая композиция представляет собой не содержащий фосфат или содержащий фосфат детергент.
27. Моющая композиция по п.19, где указанная моющая композиция представляет собой детергент для холодной воды.
28. Моющая композиция по п.19, дополнительно содержащая по меньшей мере один дополнительный фермент.
29. Моющая композиция по п.28, где указанный по меньшей мере один дополнительный фермент выбран из гемицеллюлаз, целлюлаз, пероксидаз, протеаз, маталлопротеиназ, ксиланаз, липаз, фосфолипаз, эстераз, пергидролаз, кутиназ, пектиназ, пектатлиаз, маннаназ, кератиназ, редуктаз, оксидаз, фенолоксидаз, липоксигеназ, лигниназ, пуллуланаз, танназ, пентосаназ, маланаз, β-глюканаз, арабинозидаз, гиалуронидазы, хондроитиназы, лакказы и амилаз, или их смесей.
30. Способ очистки, включающий предоставление объекта, подлежащего очистке, и композиции, содержащей моющую композицию, указанную в любом из пп.19-29, и приведение указанного объекта в контакт с указанной композицией в условиях, эффективных для обеспечения очистки указанного объекта.
31. Способ по п.30, дополнительно включающий стадию промывания указанного объекта после приведения указанного объекта в контакт с указанной моющей композицией.
32. Способ по п.30 или 31, где указанный объект, подлежащий очистке, включает посуду.
33. Способ по п.30 или 31, где указанный объект, подлежащий очистке, включает ткань.
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