RU2012116537A - Применение вариантов протеазы - Google Patents
Применение вариантов протеазы Download PDFInfo
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- RU2012116537A RU2012116537A RU2012116537/10A RU2012116537A RU2012116537A RU 2012116537 A RU2012116537 A RU 2012116537A RU 2012116537/10 A RU2012116537/10 A RU 2012116537/10A RU 2012116537 A RU2012116537 A RU 2012116537A RU 2012116537 A RU2012116537 A RU 2012116537A
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- Prior art keywords
- use according
- option
- subtilisin
- seq
- variant
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- 108091005804 Peptidases Proteins 0.000 title 1
- 239000004365 Protease Substances 0.000 title 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title 1
- 108090000787 Subtilisin Proteins 0.000 claims abstract 10
- 229920001184 polypeptide Polymers 0.000 claims abstract 8
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 8
- 102220036452 rs137882485 Human genes 0.000 claims abstract 6
- 238000006467 substitution reaction Methods 0.000 claims abstract 6
- 238000004851 dishwashing Methods 0.000 claims abstract 4
- 102220225954 rs1064795276 Human genes 0.000 claims abstract 4
- 102200029981 rs28936700 Human genes 0.000 claims abstract 4
- 102200118280 rs33918343 Human genes 0.000 claims abstract 4
- 102200034374 rs6092 Human genes 0.000 claims abstract 4
- 102220289974 rs757282628 Human genes 0.000 claims abstract 4
- 102220099575 rs878853725 Human genes 0.000 claims abstract 4
- 102220120384 rs886042566 Human genes 0.000 claims abstract 4
- 238000005406 washing Methods 0.000 claims abstract 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 2
- 238000012986 modification Methods 0.000 claims abstract 2
- 230000004048 modification Effects 0.000 claims abstract 2
- 239000004382 Amylase Substances 0.000 claims 1
- 102000013142 Amylases Human genes 0.000 claims 1
- 108010065511 Amylases Proteins 0.000 claims 1
- 108010059892 Cellulase Proteins 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 102000004882 Lipase Human genes 0.000 claims 1
- 108090001060 Lipase Proteins 0.000 claims 1
- 239000004367 Lipase Substances 0.000 claims 1
- 235000019418 amylase Nutrition 0.000 claims 1
- 235000013605 boiled eggs Nutrition 0.000 claims 1
- 229940106157 cellulase Drugs 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 claims 1
- 235000019421 lipase Nutrition 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1. Применение варианта субтилизина для мытья твердых поверхностей, где вариант содержит замены 9R, 15T, 68A, 245R и 218 {D,G,V} по сравнению с исходным субтилизином, и где положения соответствуют положениям зрелого полипептида SEQ ID NO:2 [BPN'].2. Применение по п.1, где в положении 218 осуществляют замену на D.3. Применение по п.1, где вариант дополнительно содержит по меньшей мере одну из следующих модификаций: G61{D,E}, N62{D,E}, N76{D,E}; *97aG, A98{G,S}, S99G, S101G, H120{N,V,Q,D}, P131{T,S}, Q137H, A194P, A228V, A230V, N261D.4. Применение по п.1, где вариант содержит следующие замены: S9R, A15T, V68A, N218D и Q245R.5. Применение по п.4, где вариант дополнительно содержит замену G61E.6. Применение по п.4, где вариант дополнительно содержит замену A98S.7. Применение по п.4, где вариант дополнительно содержит замену S99G.8. Применение по любому из пп.1-7, где вариант содержит следующие замены: S9R, A15T, G61E, V68A, A98S, S99G, N218D и Q245R.9. Применение по любому из пп.1-7, где исходный субтилизин принадлежит к подгруппе I-S2.10. Применение по любому из пп.1-7, где исходный субтилизин представляет собой полипептид, содержащий аминокислотную последовательность по меньшей мере на 80% идентичную SEQ ID NO:1.11. Применение по п.1, где вариант представляет собой полипептидную последовательность по меньшей мере на 80% идентичную SEQ ID NO:3.12. Применение по п.1, где вариант представляет собой полипептидную последовательность по меньшей мере на 80% идентичную SEQ ID NO:4.13. Применение по любому из пп.1-7, где вариант обладает одним или несколькими улучшенными свойствами по сравнению с исходным субтилизином, где улучшенные свойства включают улучшенные характеристики мытья твердых поверхностей, такого так мытье посуды.14. Композиция для мытья посуды, содержащая вариант по лю�
Claims (16)
1. Применение варианта субтилизина для мытья твердых поверхностей, где вариант содержит замены 9R, 15T, 68A, 245R и 218 {D,G,V} по сравнению с исходным субтилизином, и где положения соответствуют положениям зрелого полипептида SEQ ID NO:2 [BPN'].
2. Применение по п.1, где в положении 218 осуществляют замену на D.
3. Применение по п.1, где вариант дополнительно содержит по меньшей мере одну из следующих модификаций: G61{D,E}, N62{D,E}, N76{D,E}; *97aG, A98{G,S}, S99G, S101G, H120{N,V,Q,D}, P131{T,S}, Q137H, A194P, A228V, A230V, N261D.
4. Применение по п.1, где вариант содержит следующие замены: S9R, A15T, V68A, N218D и Q245R.
5. Применение по п.4, где вариант дополнительно содержит замену G61E.
6. Применение по п.4, где вариант дополнительно содержит замену A98S.
7. Применение по п.4, где вариант дополнительно содержит замену S99G.
8. Применение по любому из пп.1-7, где вариант содержит следующие замены: S9R, A15T, G61E, V68A, A98S, S99G, N218D и Q245R.
9. Применение по любому из пп.1-7, где исходный субтилизин принадлежит к подгруппе I-S2.
10. Применение по любому из пп.1-7, где исходный субтилизин представляет собой полипептид, содержащий аминокислотную последовательность по меньшей мере на 80% идентичную SEQ ID NO:1.
11. Применение по п.1, где вариант представляет собой полипептидную последовательность по меньшей мере на 80% идентичную SEQ ID NO:3.
12. Применение по п.1, где вариант представляет собой полипептидную последовательность по меньшей мере на 80% идентичную SEQ ID NO:4.
13. Применение по любому из пп.1-7, где вариант обладает одним или несколькими улучшенными свойствами по сравнению с исходным субтилизином, где улучшенные свойства включают улучшенные характеристики мытья твердых поверхностей, такого так мытье посуды.
14. Композиция для мытья посуды, содержащая вариант по любому из пп.1-13.
15. Композиция по п.14, дополнительно содержащая по меньшей мере один другой фермент, выбранный из группы, включающей липазу, амилазу и целлюлазу.
16. Применение композиции по любому из пп.14-15 для удаления белковых пятен, в особенности, пятен от вареных яиц.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09171311.5 | 2009-09-25 | ||
| EP09171311 | 2009-09-25 | ||
| PCT/EP2010/064174 WO2011036264A1 (en) | 2009-09-25 | 2010-09-24 | Use of protease variants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2012116537A true RU2012116537A (ru) | 2013-10-27 |
| RU2639534C2 RU2639534C2 (ru) | 2017-12-21 |
Family
ID=41323405
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012116537A RU2639534C2 (ru) | 2009-09-25 | 2010-09-24 | Применение вариантов протеазы |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20120252106A1 (ru) |
| EP (1) | EP2480663B1 (ru) |
| JP (1) | JP6204018B2 (ru) |
| CN (2) | CN104946427A (ru) |
| AU (1) | AU2010299800B2 (ru) |
| BR (1) | BR112012006497A2 (ru) |
| CA (1) | CA2775048A1 (ru) |
| MX (1) | MX2012003387A (ru) |
| RU (1) | RU2639534C2 (ru) |
| WO (1) | WO2011036264A1 (ru) |
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| US20070161531A1 (en) * | 2005-07-08 | 2007-07-12 | Novozymes A/S | Subtilase variants |
| US20120172280A1 (en) * | 2009-09-25 | 2012-07-05 | Novozymes A/S | Protease Variants |
| DE102011118037A1 (de) | 2011-06-16 | 2012-12-20 | Henkel Ag & Co. Kgaa | Geschirrspülmittel mit Bleichkatalysator und Protease |
| ES2582608T3 (es) * | 2012-02-17 | 2016-09-14 | Henkel Ag & Co. Kgaa | Composiciones detergentes que comprenden variantes de subtilasa |
| JP6067409B2 (ja) * | 2012-04-10 | 2017-01-25 | 花王株式会社 | アルカリプロテアーゼの溶解性向上方法 |
| MX2014013727A (es) | 2012-05-16 | 2015-02-10 | Novozymes As | Composiciones que comprenden lipasa y metodos de utilizacion de estas. |
| MX364390B (es) | 2012-06-20 | 2019-04-25 | Novozymes As | Uso de polipeptidos que tienen actividad proteasa en alimentos para animales y detergentes. |
| MX357022B (es) | 2012-08-22 | 2018-06-25 | Novozymes As | Metaloproteasas de alicyclobacillus sp. |
| BR112015003724A2 (pt) | 2012-08-22 | 2017-08-08 | Novozymes As | polipeptídeo isolado, composição, uso de uma composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, e, métodos de produção de um polipeptídeo e de produção de uma proteína. |
| MX2015002212A (es) | 2012-08-22 | 2015-05-08 | Novozymes As | Composiciones detergentes que comprenden metaloproteasas. |
| DE102012215107A1 (de) * | 2012-08-24 | 2014-02-27 | Basf Se | Festes Geschirrspülmittel mit verbesserter Proteaseleistung |
| US10323217B2 (en) | 2012-12-07 | 2019-06-18 | Novozymes A/S | Detergent composition comprising enzymes and washing method for preventing adhesion of bacteria |
| WO2014100100A1 (en) * | 2012-12-20 | 2014-06-26 | The Procter & Gamble Company | Detergent composition with silicate coated bleach |
| MX363360B (es) | 2012-12-21 | 2019-03-21 | Novozymes As | Polipéptidos que poseen actividad proteasa y polinucleótidos que los codifican. |
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| CN105209613A (zh) | 2013-05-17 | 2015-12-30 | 诺维信公司 | 具有α淀粉酶活性的多肽 |
| US10538751B2 (en) | 2013-06-06 | 2020-01-21 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
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2010
- 2010-09-24 RU RU2012116537A patent/RU2639534C2/ru not_active IP Right Cessation
- 2010-09-24 CN CN201510204996.2A patent/CN104946427A/zh active Pending
- 2010-09-24 CA CA2775048A patent/CA2775048A1/en not_active Abandoned
- 2010-09-24 AU AU2010299800A patent/AU2010299800B2/en not_active Ceased
- 2010-09-24 JP JP2012530277A patent/JP6204018B2/ja active Active
- 2010-09-24 BR BR112012006497A patent/BR112012006497A2/pt not_active Application Discontinuation
- 2010-09-24 WO PCT/EP2010/064174 patent/WO2011036264A1/en active Application Filing
- 2010-09-24 MX MX2012003387A patent/MX2012003387A/es active IP Right Grant
- 2010-09-24 EP EP10757601.9A patent/EP2480663B1/en not_active Not-in-force
- 2010-09-24 CN CN201080053391.3A patent/CN102648277B/zh active Active
- 2010-09-24 US US13/496,249 patent/US20120252106A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CN104946427A (zh) | 2015-09-30 |
| WO2011036264A1 (en) | 2011-03-31 |
| RU2639534C2 (ru) | 2017-12-21 |
| JP2013506022A (ja) | 2013-02-21 |
| CN102648277A (zh) | 2012-08-22 |
| BR112012006497A2 (pt) | 2015-09-08 |
| CA2775048A1 (en) | 2011-03-31 |
| MX2012003387A (es) | 2012-04-10 |
| US20120252106A1 (en) | 2012-10-04 |
| AU2010299800B2 (en) | 2014-08-07 |
| EP2480663A1 (en) | 2012-08-01 |
| AU2010299800A1 (en) | 2012-04-05 |
| CN102648277B (zh) | 2015-05-20 |
| JP6204018B2 (ja) | 2017-09-27 |
| EP2480663B1 (en) | 2017-11-15 |
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