AR070491A1 - Variante de enzima lipolitica con estabilidad aumentada y polinucleotidos que la codifican - Google Patents

Variante de enzima lipolitica con estabilidad aumentada y polinucleotidos que la codifican

Info

Publication number
AR070491A1
AR070491A1 ARP090100663A ARP090100663A AR070491A1 AR 070491 A1 AR070491 A1 AR 070491A1 AR P090100663 A ARP090100663 A AR P090100663A AR P090100663 A ARP090100663 A AR P090100663A AR 070491 A1 AR070491 A1 AR 070491A1
Authority
AR
Argentina
Prior art keywords
variant
lipolytic enzyme
progenitor
lipolitic
polinucleotides
Prior art date
Application number
ARP090100663A
Other languages
English (en)
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of AR070491A1 publication Critical patent/AR070491A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Abstract

Además se proporcionan un polinucleotido aislado que codifica variantes de la enzima lipolítica, un constructo de ácido nucleico, un vector de expresion, una célula huésped, un método para preparar la variante de la enzima proteolítica y el uso de la variante para la elaboracion de una formulacion estable en composiciones detergentes. Reivindicacion 1: Una variante de una enzima lipolítica progenitora, donde la variante: a) Tiene una secuencia de aminoácidos que al compararse a la enzima lipolítica progenitora comprende sustitucion de un residuo aminoácido que corresponde a cualquiera de los aminoácidos 27, 216, 227, 231, 233 y 256 de la SEC ID N°: 2; y b) es más estable en composiciones detergentes que la enzima lipolítica progenitora.
ARP090100663A 2008-02-29 2009-02-26 Variante de enzima lipolitica con estabilidad aumentada y polinucleotidos que la codifican AR070491A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08152164 2008-02-29
US3309708P 2008-03-03 2008-03-03

Publications (1)

Publication Number Publication Date
AR070491A1 true AR070491A1 (es) 2010-04-07

Family

ID=39301592

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP090100663A AR070491A1 (es) 2008-02-29 2009-02-26 Variante de enzima lipolitica con estabilidad aumentada y polinucleotidos que la codifican

Country Status (8)

Country Link
US (1) US9163203B2 (es)
EP (1) EP2250258B1 (es)
JP (1) JP2011514805A (es)
CN (1) CN101990573B (es)
AR (1) AR070491A1 (es)
CA (1) CA2715521A1 (es)
MX (2) MX2010009077A (es)
WO (1) WO2009106553A2 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103052704A (zh) 2010-07-22 2013-04-17 荷兰联合利华有限公司 用于提高清洁的鼠李糖脂和酶的组合物
DK3382003T3 (da) * 2011-12-29 2021-09-06 Novozymes As Detergentsammensætninger med lipasevarianter
EP2906691B1 (en) * 2012-10-12 2021-01-27 Danisco US Inc. Compositions and methods comprising a lipolytic enzyme variant
WO2014074352A1 (en) * 2012-11-06 2014-05-15 The Regents Of The University Of California Dieselzymes and methanol tolerant lipases
AR096270A1 (es) 2013-05-14 2015-12-16 Novozymes As Composiciones detergentes
US10023852B2 (en) 2014-05-27 2018-07-17 Novozymes A/S Lipase variants and polynucleotides encoding same
US20170121644A1 (en) * 2014-07-08 2017-05-04 Novozymes A/S Organic Film Removal From Plastic Objects
CA2965232A1 (en) * 2014-12-09 2016-06-16 Novozymes A/S Lipase variants and polynucleotides encoding same
WO2016102356A1 (en) * 2014-12-22 2016-06-30 Novozymes A/S Detergent compositions, lipase variants and polynucleotides encoding same
EP3950939A3 (en) * 2015-07-06 2022-06-08 Novozymes A/S Lipase variants and polynucleotides encoding same
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN115927250A (zh) * 2022-08-26 2023-04-07 云南师范大学 第256位点突变的疏棉状嗜热丝孢菌脂肪酶突变体及其应用

Family Cites Families (19)

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Publication number Priority date Publication date Assignee Title
ATE110768T1 (de) 1986-08-29 1994-09-15 Novo Nordisk As Enzymhaltiger reinigungsmittelzusatz.
ES2076939T3 (es) 1987-08-28 1995-11-16 Novo Nordisk As Lipasa recombinante de humicola y procedimiento para la produccion de lipasas recombinantes de humicola.
US5176796A (en) 1988-12-13 1993-01-05 Jujo Paper Co., Ltd. Avoiding pitch troubles using acylgerol lipase
US5892013A (en) * 1990-09-13 1999-04-06 Novo Nordisk A/S Lipase variants
KR930702514A (ko) * 1990-09-13 1993-09-09 안네 제케르 리파제 변체
JPH04240286A (ja) 1991-01-25 1992-08-27 Novo Nordisk As 耐熱性リパーゼによるピッチトラブル防止法
ATE169678T1 (de) 1991-05-01 1998-08-15 Novo Nordisk As Stabilisierte enzyme
DE69632538T2 (de) 1995-08-11 2005-05-19 Novozymes A/S Neuartige lipolytische enzyme
JP4523178B2 (ja) 1999-03-31 2010-08-11 ノボザイムス アクティーゼルスカブ リパーゼ変異体
AU2002219020B2 (en) * 2001-01-10 2007-05-24 Novozymes A/S Thermostable lipolytic enzyme variant
JP4287149B2 (ja) * 2001-02-07 2009-07-01 ノボザイムス アクティーゼルスカブ リパーゼ変異体
EP1851311A2 (en) 2005-02-10 2007-11-07 Novozymes A/S Enzymatic enantioselective ester or amide hydrolysis or synthesis
EP1979476B1 (en) * 2006-01-23 2016-08-10 Novozymes A/S Polypeptides having lipase activity and polynucleotides encoding same
CA2635934A1 (en) * 2006-01-23 2007-08-02 The Procter & Gamble Company Detergent compositions
EP1979477B1 (en) * 2006-01-23 2017-04-19 Novozymes A/S Lipase variants
EP1979455A2 (en) * 2006-01-23 2008-10-15 The Procter & Gamble Company Detergent compositions
WO2007087242A2 (en) * 2006-01-23 2007-08-02 The Procter & Gamble Company A composition comprising a lipase and a bleach catalyst
EP2099907A2 (en) * 2006-12-21 2009-09-16 Novozymes A/S Lipase variants for pharmaceutical use
JP5650543B2 (ja) * 2008-02-29 2015-01-07 ノボザイムス アクティーゼルスカブ リパーゼ活性を有するポリペプチド及びこれをコードするポリヌクレオチド

Also Published As

Publication number Publication date
CN101990573A (zh) 2011-03-23
JP2011514805A (ja) 2011-05-12
WO2009106553A2 (en) 2009-09-03
WO2009106553A3 (en) 2009-12-10
MX2010009077A (es) 2010-09-24
MX337307B (es) 2016-02-25
CA2715521A1 (en) 2009-09-03
CN101990573B (zh) 2014-01-22
EP2250258A2 (en) 2010-11-17
US9163203B2 (en) 2015-10-20
EP2250258B1 (en) 2017-01-04
US20090221034A1 (en) 2009-09-03

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