ES2598115T3 - Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias - Google Patents

Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias Download PDF

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Publication number
ES2598115T3
ES2598115T3 ES15154566.2T ES15154566T ES2598115T3 ES 2598115 T3 ES2598115 T3 ES 2598115T3 ES 15154566 T ES15154566 T ES 15154566T ES 2598115 T3 ES2598115 T3 ES 2598115T3
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Prior art keywords
sequence
crispr
tracr
target
polynucleotide
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English (en)
Spanish (es)
Inventor
Feng Zhang
Le Cong
Patrick Hsu
Fei RAN
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Massachusetts Institute of Technology
Broad Institute Inc
Harvard University
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Massachusetts Institute of Technology
Broad Institute Inc
Harvard University
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    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Y—ENZYMES
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    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
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    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
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    • C12N2800/00—Nucleic acids vectors
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  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
ES15154566.2T 2012-12-12 2013-12-12 Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias Active ES2598115T3 (es)

Applications Claiming Priority (24)

Application Number Priority Date Filing Date Title
US201261736527P 2012-12-12 2012-12-12
US201261736527P 2012-12-12
US201361748427P 2013-01-02 2013-01-02
US201361748427P 2013-01-02
US201361758468P 2013-01-30 2013-01-30
US201361758468P 2013-01-30
US201361769046P 2013-02-25 2013-02-25
US201361769046P 2013-02-25
US201361791409P 2013-03-15 2013-03-15
US201361802174P 2013-03-15 2013-03-15
US201361791409P 2013-03-15
US201361802174P 2013-03-15
US201361806375P 2013-03-28 2013-03-28
US201361806375P 2013-03-28
US201361814263P 2013-04-20 2013-04-20
US201361814263P 2013-04-20
US201361819803P 2013-05-06 2013-05-06
US201361819803P 2013-05-06
US201361828130P 2013-05-28 2013-05-28
US201361828130P 2013-05-28
US201361836127P 2013-06-17 2013-06-17
US201361835931P 2013-06-17 2013-06-17
US201361835931P 2013-06-17
US201361836127P 2013-06-17

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ES2598115T3 true ES2598115T3 (es) 2017-01-25

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ES15154566.2T Active ES2598115T3 (es) 2012-12-12 2013-12-12 Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias
ES14170383.5T Active ES2542015T3 (es) 2012-12-12 2013-12-12 Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias
ES13818570.7T Active ES2536353T3 (es) 2012-12-12 2013-12-12 Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias
ES15154539.9T Active ES2553782T3 (es) 2012-12-12 2013-12-12 Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias

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