CA3149293C - Droplet-based assay system - Google Patents

Droplet-based assay system Download PDF

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Publication number
CA3149293C
CA3149293C CA3149293A CA3149293A CA3149293C CA 3149293 C CA3149293 C CA 3149293C CA 3149293 A CA3149293 A CA 3149293A CA 3149293 A CA3149293 A CA 3149293A CA 3149293 C CA3149293 C CA 3149293C
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Canada
Prior art keywords
droplets
sample
droplet
fluid
chamber
Prior art date
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CA3149293A
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English (en)
French (fr)
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CA3149293A1 (en
Inventor
Billy Wayne Colston, Jr
Benjamin Joseph Hindson
Kevin Dean Ness
Donald Arthur Masquelier
Fred Paul Milanovich
Douglas N. Modlin
Vincent Riot
Samuel Burd
Anthony Joseph Makarewicz, Jr
Phillip Belgrader
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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Priority to CA3210271A priority Critical patent/CA3210271A1/en
Publication of CA3149293A1 publication Critical patent/CA3149293A1/en
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Publication of CA3149293C publication Critical patent/CA3149293C/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
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    • B01F25/30—Injector mixers
    • B01F25/31—Injector mixers in conduits or tubes through which the main component flows
    • B01F25/314—Injector mixers in conduits or tubes through which the main component flows wherein additional components are introduced at the circumference of the conduit
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    • B01F33/3011—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
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    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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