AU2008275915B2 - Method for generating aptamers with improved off-rates - Google Patents

Method for generating aptamers with improved off-rates Download PDF

Info

Publication number
AU2008275915B2
AU2008275915B2 AU2008275915A AU2008275915A AU2008275915B2 AU 2008275915 B2 AU2008275915 B2 AU 2008275915B2 AU 2008275915 A AU2008275915 A AU 2008275915A AU 2008275915 A AU2008275915 A AU 2008275915A AU 2008275915 B2 AU2008275915 B2 AU 2008275915B2
Authority
AU
Australia
Prior art keywords
slow
rate
aptamer
target
minutes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2008275915A
Other languages
English (en)
Other versions
AU2008275915A1 (en
AU2008275915A2 (en
Inventor
Chris Bock
Bruce Eaton
Larry Gold
Daniel J. Schneider
Sheri K. Wilcox
Dominic Zichi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Somalogic Operating Co Inc
Original Assignee
Somalogic Operating Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40260081&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2008275915(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Somalogic Operating Co Inc filed Critical Somalogic Operating Co Inc
Publication of AU2008275915A1 publication Critical patent/AU2008275915A1/en
Publication of AU2008275915A2 publication Critical patent/AU2008275915A2/en
Priority to AU2013203374A priority Critical patent/AU2013203374B2/en
Priority to AU2013203386A priority patent/AU2013203386B2/en
Publication of AU2008275915B2 publication Critical patent/AU2008275915B2/en
Application granted granted Critical
Priority to AU2015249081A priority patent/AU2015249081B2/en
Priority to AU2015249082A priority patent/AU2015249082B2/en
Priority to AU2017202051A priority patent/AU2017202051B2/en
Priority to AU2019201066A priority patent/AU2019201066B2/en
Assigned to SOMALOGIC OPERATING CO., INC. reassignment SOMALOGIC OPERATING CO., INC. Request for Assignment Assignors: SOMALOGIC, INC.
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C12N2310/3341—5-Methylcytosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2541/00—Reactions characterised by directed evolution
    • C12Q2541/10—Reactions characterised by directed evolution the purpose being the selection or design of target specific nucleic acid binding sequences
    • C12Q2541/101—Selex

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU2008275915A 2007-07-17 2008-07-17 Method for generating aptamers with improved off-rates Active AU2008275915B2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2013203386A AU2013203386B2 (en) 2007-07-17 2013-04-10 Method for generating aptamers with improved off-rates
AU2013203374A AU2013203374B2 (en) 2007-07-17 2013-04-10 Method for generating aptamers with improved off-rates
AU2015249082A AU2015249082B2 (en) 2007-07-17 2015-10-28 Method for generating aptamers with improved off-rates
AU2015249081A AU2015249081B2 (en) 2007-07-17 2015-10-28 Method for generating aptamers with improved off-rates
AU2017202051A AU2017202051B2 (en) 2007-07-17 2017-03-28 Method for generating aptamers with improved off-rates
AU2019201066A AU2019201066B2 (en) 2007-07-17 2019-02-15 Method for generating aptamers with improved off-rates

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US95029307P 2007-07-17 2007-07-17
US95028307P 2007-07-17 2007-07-17
US95028107P 2007-07-17 2007-07-17
US60/950,283 2007-07-17
US60/950,281 2007-07-17
US60/950,293 2007-07-17
US3142008P 2008-02-26 2008-02-26
US61/031,420 2008-02-26
US5159408P 2008-05-08 2008-05-08
US61/051,594 2008-05-08
PCT/US2008/070383 WO2009012418A2 (en) 2007-07-17 2008-07-17 Method for generating aptamers with improved off-rates

Related Child Applications (2)

Application Number Title Priority Date Filing Date
AU2013203374A Division AU2013203374B2 (en) 2007-07-17 2013-04-10 Method for generating aptamers with improved off-rates
AU2013203386A Division AU2013203386B2 (en) 2007-07-17 2013-04-10 Method for generating aptamers with improved off-rates

Publications (3)

Publication Number Publication Date
AU2008275915A1 AU2008275915A1 (en) 2009-01-22
AU2008275915A2 AU2008275915A2 (en) 2010-04-22
AU2008275915B2 true AU2008275915B2 (en) 2013-07-11

Family

ID=40260081

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2008275915A Active AU2008275915B2 (en) 2007-07-17 2008-07-17 Method for generating aptamers with improved off-rates
AU2008275917A Active AU2008275917B2 (en) 2007-07-17 2008-07-17 Multiplexed analyses of test samples
AU2008276001A Abandoned AU2008276001A1 (en) 2007-07-17 2008-07-17 Improved selex and photoselex

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2008275917A Active AU2008275917B2 (en) 2007-07-17 2008-07-17 Multiplexed analyses of test samples
AU2008276001A Abandoned AU2008276001A1 (en) 2007-07-17 2008-07-17 Improved selex and photoselex

Country Status (17)

Country Link
US (1) US8409795B2 (enExample)
EP (10) EP2172566B2 (enExample)
JP (11) JP5684568B2 (enExample)
KR (3) KR101747665B1 (enExample)
CN (6) CN104593373A (enExample)
AU (3) AU2008275915B2 (enExample)
CA (4) CA2693448A1 (enExample)
DK (4) DK2172566T4 (enExample)
ES (6) ES2537322T5 (enExample)
FI (1) FI3284832T3 (enExample)
HR (1) HRP20150382T1 (enExample)
MX (7) MX2010000578A (enExample)
NO (1) NO2933340T3 (enExample)
PL (1) PL2489743T3 (enExample)
PT (2) PT2489743E (enExample)
SI (1) SI2489743T1 (enExample)
WO (3) WO2009012420A1 (enExample)

Families Citing this family (137)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5993279A (ja) * 1982-11-17 1984-05-29 富士通株式会社 ロボツトによるカ−ド，レシ−ト分離取出し方法
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US7964356B2 (en) * 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
SI2489743T1 (sl) * 2007-07-17 2015-04-30 Somalogic, Inc. Aptameri s 5-(N-naftilno) substituiranimi uridini
US8404830B2 (en) * 2007-07-17 2013-03-26 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8314052B2 (en) * 2009-03-23 2012-11-20 Base Pair Biotechnologies, Inc. Methods for simultaneous generation of functional ligands
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
WO2010111265A1 (en) 2009-03-24 2010-09-30 University Of Chicago Slip chip device and methods
US8569252B2 (en) * 2009-04-15 2013-10-29 Postech Academy-Industry Foundation Nucleolin specific aptamer and use thereof
AU2013203588C1 (en) * 2009-07-09 2016-06-30 Somalogic Operating Co., Inc. Method for generating aptamers with improved off-rates
JP5967687B2 (ja) * 2009-07-17 2016-08-10 Ｎｅｃソリューションイノベータ株式会社 スフィンゴシルホスホリルコリンに結合するアプタマー分子
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
US9453845B2 (en) 2010-02-01 2016-09-27 Cell Signaling Technology, Inc. Mass spectroscopy analysis of mutant polypeptides in biological samples
US9080171B2 (en) * 2010-03-24 2015-07-14 RXi Parmaceuticals Corporation Reduced size self-delivering RNAi compounds
EP2558586B1 (en) * 2010-04-12 2016-10-12 Somalogic, Inc. APTAMERS TO ß-NGF AND THEIR USE IN TREATING ß-NGF MEDIATED DISEASES AND DISORDERS
AU2013202528B2 (en) * 2010-04-12 2015-07-30 Somalogic Operating Co., Inc. 5-position modified pyrimidines and their use
MX355020B (es) * 2010-07-09 2018-04-02 Somalogic Inc Biomarcadores de cancer de pulmon y usos de los mismos.
AU2011312491B2 (en) 2010-09-27 2015-06-04 Somalogic, Inc. Mesothelioma biomarkers and uses thereof
AU2011323085B2 (en) 2010-11-05 2014-10-30 Miragen Therapeutics Base modified oligonucleotides
KR101307616B1 (ko) * 2011-02-08 2013-09-12 경희대학교 산학협력단 Ｐｎａ 앱타머를 이용하여 금속을 분리하는 방법
CN103958683A (zh) 2011-12-28 2014-07-30 希森美康株式会社 与肾上腺皮质刺激激素结合的分子及其应用
MX355567B (es) 2011-12-30 2018-04-23 Quest Diagnostics Invest Inc Aptameros y metodos de diagnostico para detectar el receptor de egf.
WO2013149086A1 (en) 2012-03-28 2013-10-03 Somalogic, Inc. Aptamers to pdgf and vegf and their use in treating pdgf and vegf mediated conditions
CN104508492B (zh) * 2012-05-25 2018-07-27 北卡罗来纳-查佩尔山大学 微流体装置、用于试剂的固体支持体和相关方法
US20150141259A1 (en) * 2012-06-07 2015-05-21 Somalogic, Inc. Aptamer-Based Multiplexed Assays
CN102719430B (zh) * 2012-06-13 2013-06-12 湖南大学 一种检测组氨酸标签重组蛋白的核酸适配体分子信标探针及其检测方法
WO2014007438A1 (ko) 2012-07-02 2014-01-09 국립대학법인 울산과학기술대학교 산학협력단 페리오스틴에 대한 압타머 및 이를 포함하는 항암제 조성물
EP2690441A1 (en) * 2012-07-26 2014-01-29 Rheinische Friedrich-Wilhelms-Universität Bonn Method of determining a protein in a sample
CN102912020B (zh) * 2012-10-20 2014-02-19 江南大学 一种测定赭曲霉毒素a的适配体传感器的构建方法
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
EP2912182B1 (en) 2012-10-23 2021-12-08 Caris Science, Inc. Aptamers and uses thereof
WO2014074682A1 (en) 2012-11-07 2014-05-15 Somalogic, Inc. Chronic obstructive pulmonary disease (copd) biomarkers and uses thereof
WO2014100434A1 (en) 2012-12-19 2014-06-26 Caris Science, Inc. Compositions and methods for aptamer screening
WO2014159669A2 (en) 2013-03-14 2014-10-02 Somalogic, Inc. Aptamers that bind to il-6 and their use in treating or diagnosing il-6 mediated conditions
WO2014144744A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Aptamer methods and compositions
BR122019026188B1 (pt) 2013-03-15 2024-01-02 Somalogic Operating Co., Inc Métodos para determinar se um indivíduo com esteatose não alcoólica tem esteato-hepatite não alcoólica e para monitorar um indivíduo com esteatose não alcoólica para o desenvolvimento de nash
JP6034261B2 (ja) * 2013-08-02 2016-11-30 日本電信電話株式会社 生体分子検出用チップの製造方法
CA3221709A1 (en) * 2013-09-09 2015-03-12 Somalogic Operating Co., Inc. Pdgf and vegf aptamers having improved stability and their use in treating pdgf and vegf mediated diseases and disorders
CA2924987C (en) 2013-09-24 2022-08-16 Somalogic, Inc. Multiaptamer target detection
ES2714324T3 (es) * 2013-10-02 2019-05-28 Fabian Tolle Un método para identificar o producir un aptámero
BR112016010165A2 (pt) 2013-11-21 2017-12-05 Somalogic Inc composições de nucleotídeos modificados de citidina-5-carboxamida e métodos relacionados às mesmas
US10093933B2 (en) 2014-02-18 2018-10-09 Somalogic, Inc. Compositions and methods for detecting microorganisms
WO2015164617A1 (en) 2014-04-24 2015-10-29 Somalogic, Inc. Tuberculosis biomarkers in urine and uses thereof
WO2015164616A1 (en) 2014-04-24 2015-10-29 Somalogic, Inc. Biomarkers for detection of tuberculosis
CN107656085B (zh) * 2014-07-01 2021-04-09 深圳迈瑞生物医疗电子股份有限公司 一种血液检测仪
AU2014407088B2 (en) 2014-09-26 2021-09-23 Somalogic Operating Co., Inc. Cardiovascular risk event prediction and uses thereof
WO2016053015A1 (ko) * 2014-09-29 2016-04-07 동국대학교 산학협력단 신규 세포 타겟 압타머 제조방법
ES2768723T3 (es) * 2014-10-02 2020-06-23 Univ Bonn Rheinische Friedrich Wilhelms Un método para identificar o producir un aptámero
KR101719285B1 (ko) 2014-11-04 2017-03-23 한국과학기술원 표적 물질에 의해 조절되는 핵산 중합효소 활성을 이용한 생체물질의 검출 및 정량 방법
AU2015353767B2 (en) 2014-11-24 2021-09-09 Somalogic Operating Co., Inc. Nucleic acid compounds for binding growth differentiation factor 11
CN104360077A (zh) * 2014-11-25 2015-02-18 重庆市科学技术研究院 一种用于检测多西环素残留的适配体核酸探针试剂盒、制备方法及其应用
WO2016123058A1 (en) 2015-01-27 2016-08-04 Somalogic, Inc. Biomarkers for detection of tuberculosis risk
WO2016130414A1 (en) 2015-02-09 2016-08-18 Somalogic, Inc. Nucleic acid compounds for binding growth differentiation factor 8
US10138486B2 (en) 2015-03-12 2018-11-27 The Regents Of The University Of Michigan Inhibitors of DEK protein and related methods
WO2016182967A1 (en) 2015-05-08 2016-11-17 Somalogic, Inc. Biomarkers for detection of tuberculosis risk
WO2017044715A1 (en) 2015-09-09 2017-03-16 Somalogic, Inc. Methods for developing personalized drug treatment plans and targeted drug development based on proteomic profiles
CN108473574A (zh) * 2015-10-20 2018-08-31 索伦托治疗有限公司 细胞内递送化合物
US20210215711A1 (en) 2016-02-08 2021-07-15 Somalogic, Inc. Nonalcoholic Fatty Liver Disease (NAFLD) and Nonalcoholic Steatohepatitis (NASH) Biomarkers and Uses Thereof
GB201603789D0 (en) 2016-03-04 2016-04-20 Apta Biosciences Ltd Oligonucleotides and methods for preparing
ES3013534T3 (en) 2016-07-01 2025-04-14 Somalogic Operating Co Inc Oligonucleotides comprising modified nucleosides
CN106248767B (zh) * 2016-07-15 2018-12-07 济南大学 一种用于检测癌细胞中h2s的三维纸分析器件的制备方法
CN107663220B (zh) * 2016-07-27 2020-10-02 上海伯豪医学检验所有限公司 修饰碱基、包含修饰碱基的核酸、适配体及其应用
JPWO2018038076A1 (ja) * 2016-08-22 2019-06-20 日産化学株式会社 Ｅ−カドヘリン結合型核酸アプタマーを用いる細胞足場材料
US11041849B2 (en) * 2016-12-01 2021-06-22 Aptitude Medical Systems, Inc. Methods and systems for identifying candidate nucleic acid agent
KR101993427B1 (ko) * 2017-04-26 2019-10-01 주식회사 압타머사이언스 백혈구에 선택적으로 결합하는 압타머 및 이의 용도
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
US10481155B2 (en) * 2018-03-09 2019-11-19 Somalogic, Inc. Proteomic assay using quantum sensors
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
US11231420B2 (en) 2018-04-09 2022-01-25 Aptalogic, Inc. Selection and optimization of aptamers to recognize ebola markers
WO2019202448A1 (en) 2018-04-16 2019-10-24 University Of Cape Town A three-protein proteomic biomarker for prospective determination of risk for development of active tuberculosis
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
AT521238B1 (de) 2018-05-09 2020-02-15 Lifetaq Analytics Gmbh In-situ zellanalyse im zellkultursystem
CA3104041A1 (en) * 2018-06-22 2019-12-26 Somalogic, Inc. Improved proteomic multiplex assays
WO2020037250A2 (en) * 2018-08-17 2020-02-20 The Trustees Of Columbia University In The City Of New York Stem-loop receptor-based field-effect transistor sensor devices target detection for small-molecule under physiological salt concentrations
WO2020068547A1 (en) 2018-09-25 2020-04-02 Siemens Healthcare Diagnostics Inc. Methods and compositions for removing biotin interference from assays using conjugated molecular traps
CN112703399B (zh) 2018-09-25 2024-10-18 美国西门子医学诊断股份有限公司 用于从使用分子阱的测定除去生物素干扰的方法和组合物
US20210338158A1 (en) 2018-10-02 2021-11-04 WearOptimo Pty Ltd Measurement system
WO2020069569A1 (en) 2018-10-02 2020-04-09 WearOptimo Pty Ltd Actuator system
US20220411783A1 (en) 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
CA3115996A1 (en) 2018-10-15 2020-04-23 Somalogic, Inc. Nucleic acid compounds for binding immunoglobulin g
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
WO2020117914A1 (en) 2018-12-04 2020-06-11 Roche Sequencing Solutions, Inc. Spatially oriented quantum barcoding of cellular targets
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
EP3942023A1 (en) 2019-03-18 2022-01-26 The Broad Institute, Inc. Compositions and methods for modulating metabolic regulators of t cell pathogenicity
JP7748877B2 (ja) 2019-05-17 2025-10-03 ソマロジック オペレーティング カンパニー インコーポレイテッド 複雑な生物学的マトリックスにおける試料間分析物変動性の制御
WO2020243661A1 (en) 2019-05-31 2020-12-03 The Broad Institute, Inc. Methods for treating metabolic disorders by targeting adcy5
US11198908B2 (en) 2019-06-17 2021-12-14 Biorchestra Co., Ltd. Method for diagnosis of Alzheimer's disease using microRNA
KR102113078B1 (ko) * 2019-07-05 2020-05-20 광주과학기술원 압타머의 선별 방법
WO2021030627A1 (en) 2019-08-13 2021-02-18 The General Hospital Corporation Methods for predicting outcomes of checkpoint inhibition and treatment thereof
CN110396536A (zh) * 2019-08-14 2019-11-01 东南大学 一种基于分支滚环扩增的外泌体荧光检测传感器
US12421557B2 (en) 2019-08-16 2025-09-23 The Broad Institute, Inc. Methods for predicting outcomes and treating colorectal cancer using a cell atlas
JP7712913B2 (ja) 2019-09-03 2025-07-24 ソマロジック オペレーティング カンパニー インコーポレイテッド 心血管リスク／イベントの予測及びその使用
CN110618265B (zh) * 2019-09-12 2023-04-18 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） 一种沙丁胺醇的可视化检测方法
US12195725B2 (en) 2019-10-03 2025-01-14 Dana-Farber Cancer Institute, Inc. Compositions and methods for modulating and detecting tissue specific TH17 cell pathogenicity
US11981922B2 (en) 2019-10-03 2024-05-14 Dana-Farber Cancer Institute, Inc. Methods and compositions for the modulation of cell interactions and signaling in the tumor microenvironment
US11793787B2 (en) 2019-10-07 2023-10-24 The Broad Institute, Inc. Methods and compositions for enhancing anti-tumor immunity by targeting steroidogenesis
CA3154410A1 (en) 2019-10-16 2021-04-22 Somalogic Operating Co., Inc. Nucleic acid compounds that bind to retinoic acid-inducible gene i protein
JP2023504683A (ja) * 2019-12-03 2023-02-06 キム・ソンチョン 試料の標的分子集団に対するプロファイルを得る方法
JP2023509677A (ja) * 2020-01-10 2023-03-09 ソマロジック オペレーティング カンパニー インコーポレイテッド 耐糖能異常障害を決定する方法
US12165747B2 (en) 2020-01-23 2024-12-10 The Broad Institute, Inc. Molecular spatial mapping of metastatic tumor microenvironment
CA3166923A1 (en) 2020-02-10 2021-08-19 Somalogic Operating Co., Inc. Nonalcoholic steatohepatitis (nash) biomarkers and uses thereof
CN111304202B (zh) * 2020-02-26 2023-03-28 西安交通大学 一种用于识别且增强hmgb1生物学活性的核酸适配子及其应用
CN115916365A (zh) * 2020-04-22 2023-04-04 宽腾矽公司 用于测序的装置和方法
CN113624724A (zh) * 2020-05-07 2021-11-09 廖世奇 一种适配体分子信标对靶分子的多元检测分析方法
JP2023526323A (ja) * 2020-05-18 2023-06-21 シャンハイ ポラリス バイオロジー カンパニー， リミテッド マイクロビーズおよびその使用
CN111735869A (zh) * 2020-05-29 2020-10-02 中山大学 一种蛋白质的检测试剂及检测方法
AU2021321586A1 (en) 2020-08-07 2023-03-16 Dana-Farber Cancer Institute, Inc. Therapeutic targeting of phosphate dysregulation in cancer via the XPR1:KIDINS220 protein complex
WO2022086913A2 (en) 2020-10-20 2022-04-28 Somalogic Operating Co., Inc. Cardiovascular event risk prediction
MX2023012081A (es) 2021-04-13 2023-10-25 Somalogic Operating Co Inc Nucleosidos modificados.
US20240218374A1 (en) 2021-04-14 2024-07-04 Somalogic Operating Co., Inc. Nucleic Acid Compounds That Bind Coronavirus Proteins
WO2022235976A1 (en) * 2021-05-06 2022-11-10 Systems Oncology, Llc Multitargeting rna compositions
EP4356140A2 (en) 2021-06-15 2024-04-24 SomaLogic Operating Co., Inc. Renal insufficiency prediction and uses thereof
EP4413372A1 (en) 2021-10-07 2024-08-14 SomaLogic Operating Co., Inc. Lung cancer prediction and uses thereof
CN114058613B (zh) * 2021-11-18 2023-10-27 广州血液中心(中国医学科学院输血研究所广州分所、广州器官移植配型中心) 一种大体积、高灵敏度核酸提取方法
CN114295594B (zh) * 2021-12-06 2023-09-19 贵州理工学院 一种基于分子信标筛选三螺旋DNA嵌入剂的“turn on”型荧光传感器
JP2025505923A (ja) 2022-01-21 2025-03-05 ソマロジック オペレーティング カンパニー インコーポレイテッド 試料品質評価の方法
WO2023183873A1 (en) 2022-03-24 2023-09-28 Mensura Health Inc. Aptamers and uses thereof
CN118829884A (zh) 2022-04-24 2024-10-22 私募蛋白质体操作有限公司 用于样品质量评估的方法
KR20250003612A (ko) 2022-04-24 2025-01-07 소마로직 오퍼레이팅 컴퍼니, 인코포레이티드 샘플 품질 평가 방법
AU2023263246A1 (en) 2022-04-24 2024-09-19 Somalogic Operating Co., Inc. Methods for sample quality assessment
CA3246311A1 (en) 2022-04-24 2023-11-02 Somalogic Operating Co Inc METHODS FOR EVALUATING THE QUALITY OF A SAMPLE
AU2023307004A1 (en) 2022-07-14 2024-11-14 Somalogic Operating Co., Inc. Methods for sample quality assessment
WO2024015485A1 (en) 2022-07-14 2024-01-18 Somalogic Operating Co., Inc. Methods of assessing dementia risk
WO2024064322A2 (en) 2022-09-23 2024-03-28 Somalogic Operating Co., Inc. Methods of assessing tobacco use status
WO2024192141A1 (en) 2023-03-13 2024-09-19 Dana-Farber Cancer Institute, Inc. Treatment of cancers having a drug-resistant mesenchymal cell state
US20240424107A1 (en) 2023-04-07 2024-12-26 POSTECH Research and Business Development Foundation Preparation of site-specific polypeptide conjugates
WO2025049331A2 (en) * 2023-08-31 2025-03-06 Illumina, Inc. Aptamer detection techniques
WO2025129158A1 (en) 2023-12-15 2025-06-19 The Broad Institute, Inc. Engineered arc delivery vesicles and uses thereof
EP4603584A1 (en) * 2024-02-16 2025-08-20 Leica Microsystems CMS GmbH Affinity reagent, marker and method for analysing a biological sample
WO2025250808A1 (en) 2024-05-29 2025-12-04 The Brigham And Women’S Hospital, Inc. Anti-crispr delivery compositions and methods
CN119804610B (zh) * 2024-12-27 2025-11-07 南京师范大学 一种测定适配体和小分子靶标间相互作用的非变性质谱检测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070041901A1 (en) * 2002-06-18 2007-02-22 Diener John L Stabilized aptamers to PSMA and their use as prostate cancer therapeutics

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
WO1986005803A1 (fr) 1985-03-30 1986-10-09 Marc Ballivet Procede d'obtention d'adn, arn, peptides, polypeptides ou proteines, par une technique de recombinaison d'adn
US4753983A (en) 1986-05-07 1988-06-28 Bioprobe International, Inc. Polymeric matrix for affinity chromatography and immobilization of ligands
WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5496938A (en) 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US5763173A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US6346611B1 (en) 1990-06-11 2002-02-12 Gilead Sciences, Inc. High affinity TGfβ nucleic acid ligands and inhibitors
US5707796A (en) 1990-06-11 1998-01-13 Nexstar Pharmaceuticals, Inc. Method for selecting nucleic acids on the basis of structure
US5763177A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5705337A (en) 1990-06-11 1998-01-06 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US20040132067A1 (en) 1990-06-11 2004-07-08 Somalogic, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US6168778B1 (en) 1990-06-11 2001-01-02 Nexstar Pharmaceuticals, Inc. Vascular endothelial growth factor (VEGF) Nucleic Acid Ligand Complexes
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5472841A (en) 1990-06-11 1995-12-05 Nexstar Pharmaceuticals, Inc. Methods for identifying nucleic acid ligands of human neutrophil elastase
US6001577A (en) 1998-06-08 1999-12-14 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5874557A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5789163A (en) 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
US5962219A (en) 1990-06-11 1999-10-05 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-selex
US5580737A (en) 1990-06-11 1996-12-03 Nexstar Pharmaceuticals, Inc. High-affinity nucleic acid ligands that discriminate between theophylline and caffeine
US5853984A (en) 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
US6083696A (en) * 1990-06-11 2000-07-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands exponential enrichment: blended selex
US5861254A (en) * 1997-01-31 1999-01-19 Nexstar Pharmaceuticals, Inc. Flow cell SELEX
US5874218A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound in a substance using a nucleic acid ligand
KR970002255B1 (ko) 1990-06-11 1997-02-26 넥스스타 파아마슈티컬드, 인크. 핵산 리간드
US5567588A (en) 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5459015A (en) 1990-06-11 1995-10-17 Nexstar Pharmaceuticals, Inc. High-affinity RNA ligands of basic fibroblast growth factor
US6232071B1 (en) * 1990-06-11 2001-05-15 Gilead Sciences, Inc. Tenascin-C nucleic acid ligands
US6716580B2 (en) * 1990-06-11 2004-04-06 Somalogic, Inc. Method for the automated generation of nucleic acid ligands
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US6030776A (en) * 1990-06-11 2000-02-29 Nexstar Pharmaceuticals, Inc. Parallel SELEX
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
SE501013C2 (sv) 1990-10-01 1994-10-17 Pharmacia Biosensor Ab Förfarande för förbättring av analys med bindning till fast fas
IE920562A1 (en) * 1991-02-21 1992-08-26 Gilead Sciences Aptamer specific for biomolecules and method of making
AU2580892A (en) 1991-09-05 1993-04-05 Isis Pharmaceuticals, Inc. Determination of oligonucleotides for therapeutics, diagnostics and research reagents
CA2122365C (en) * 1991-11-26 2010-05-11 Brian Froehler Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5312730A (en) * 1992-05-27 1994-05-17 Ciba Corning Diagnostics Corp. Immune complex transfer with lypophilic bridge
US5756291A (en) * 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
US5985548A (en) 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
US5428149A (en) 1993-06-14 1995-06-27 Washington State University Research Foundation Method for palladium catalyzed carbon-carbon coulping and products
US5580972A (en) 1993-06-14 1996-12-03 Nexstar Pharmaceuticals, Inc. Purine nucleoside modifications by palladium catalyzed methods
US5719273A (en) 1993-06-14 1998-02-17 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modifications methods using nucleophiles and carbon monoxide
EP1793006A3 (en) * 1993-09-08 2007-08-22 Gilead Sciences, Inc. Nucleic acid ligands and improved methods for producing the same
US5998142A (en) 1993-09-08 1999-12-07 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
AU692185B2 (en) 1993-09-17 1998-06-04 Somalogic, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US6458539B1 (en) 1993-09-17 2002-10-01 Somalogic, Inc. Photoselection of nucleic acid ligands
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
JP4899014B2 (ja) * 1995-06-02 2012-03-21 イーシー・テクノロジー・エルエルシー 求核試薬および一酸化炭素を用いるパラジウム触媒ヌクレオシド修飾方法
PT833944E (pt) 1995-06-07 2009-04-14 Gilead Sciences Inc Ligandos de ácido nucleico que se ligam a, e inibem, adn-polimerases
US6183967B1 (en) * 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
US5737498A (en) 1995-07-11 1998-04-07 Beckman Instruments, Inc. Process automation method and apparatus
AU730154B2 (en) * 1995-10-27 2001-03-01 Elliot R. Ramberg Methods and compositions for detection of specific nucleotide sequences
US5727498A (en) * 1996-02-13 1998-03-17 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Retractable visual indicator assembly
US5945527A (en) 1996-05-30 1999-08-31 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modification methods using nucleophiles and carbon monoxide
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6051698A (en) 1997-06-06 2000-04-18 Janjic; Nebojsa Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6426335B1 (en) 1997-10-17 2002-07-30 Gilead Sciences, Inc. Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
AU1252099A (en) 1997-11-26 1999-06-15 Medical Research Council Improved selex procedure and an anti-cd4 aptamer
AU3909199A (en) 1997-12-15 1999-07-05 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
US5989823A (en) 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US20070166741A1 (en) * 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US6175001B1 (en) * 1998-10-16 2001-01-16 The Scripps Research Institute Functionalized pyrimidine nucleosides and nucleotides and DNA's incorporating same
US20030054360A1 (en) 1999-01-19 2003-03-20 Larry Gold Method and apparatus for the automated generation of nucleic acid ligands
US6329145B1 (en) 1999-02-09 2001-12-11 Gilead Science, Inc. Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
US6376190B1 (en) 2000-09-22 2002-04-23 Somalogic, Inc. Modified SELEX processes without purified protein
US6933114B2 (en) * 2000-10-16 2005-08-23 Gilead Sciences, Inc. Nucleic acid ligands to the prostate specific membrane antigen
WO2002044726A1 (fr) * 2000-12-01 2002-06-06 International Reagents Corporation Procede de mesure ultrarapide et ultrasensible
WO2003014369A1 (en) 2001-08-09 2003-02-20 Somalogic, Inc. Nucleic acid ligands with intramolecular duplexes
EP1481090A4 (en) * 2002-02-15 2006-08-09 Somalogic Inc METHOD AND REAGENTS FOR DETECTING BINDING OF TARGET MOLECULES BY NUCLEIC ACID ELECTRODES
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
EP1489171B1 (en) * 2002-03-19 2012-10-31 Fujitsu Limited Functional molecule and process for producing the same
US20030228603A1 (en) * 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same
DE10241938A1 (de) * 2002-09-10 2004-03-25 Noxxon Pharma Ag Verfahren zur Selektion von Nukleinsäureliganden
CA2407825A1 (en) * 2002-10-11 2004-04-11 Andrew J. Simmonds Trap-tagging: a novel method for the identification and purification of rna-protein complexes
JP2004307464A (ja) * 2002-10-23 2004-11-04 Sankyo Co Ltd 糖部ｓ型配座の新規人工核酸
GB0226374D0 (en) * 2002-11-12 2002-12-18 Isis Innovation Ligands
JP2006517790A (ja) * 2003-01-09 2006-08-03 インヴィトロジェン コーポレーション ポリペプチド−核酸複合体の細胞の送達および活性化
WO2004085049A2 (en) * 2003-03-28 2004-10-07 The Regents Of The University Of California Preparation and application of encoded bead aggregates in combinatorial chemistry
US7672786B2 (en) 2003-07-02 2010-03-02 Sergey Krylov Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development
AU2004267420A1 (en) * 2003-08-11 2005-03-03 Monogram Biosciences, Inc. Detecting and profiling molecular complexes
KR100540509B1 (ko) * 2004-01-07 2006-01-11 학교법인 포항공과대학교 수화젤레이터로 사용되는 2'-데옥시우리딘 유도체
EP1584923A3 (en) * 2004-04-07 2006-01-04 Roche Diagnostics GmbH Stabilization of biomolecules in samples
JP4839051B2 (ja) * 2004-10-08 2011-12-14 シスメックス株式会社 核酸プローブを用いる被検物質の検出方法
WO2006096754A2 (en) * 2005-03-07 2006-09-14 Archemix Corp. Stabilized aptamers to psma and their use as prostate cancer therapeutics
JP4368412B2 (ja) * 2005-06-24 2009-11-18 マグ エアロスペース インダストリーズ インコーポレイテッド 軽度汚水インターフェイスバルブ装置および方法
WO2007018843A2 (en) * 2005-07-01 2007-02-15 Arbor Vita Corporation Methods and compositions for diagnosis and treatment of influenza
WO2007024676A2 (en) * 2005-08-19 2007-03-01 Nanosphere, Inc. Methods for preparing hybrid substrates comprising dna and antibodies and uses thereof
SI1994171T1 (sl) * 2006-01-17 2015-07-31 Somalogic, Inc. Multipleksirane analize testnih vzorcev
EP2207891B1 (en) * 2006-12-22 2012-07-25 Archemix LLC Materials and methods for the generation of transcripts comprising modified nucleotides
NZ578255A (en) * 2007-01-23 2012-06-29 Jostens Inc Method and system for creating customized output
SI2489743T1 (sl) 2007-07-17 2015-04-30 Somalogic, Inc. Aptameri s 5-(N-naftilno) substituiranimi uridini
JP2013220095A (ja) * 2012-04-12 2013-10-28 Toyohiro Fujita 苗床
JP2018078250A (ja) 2016-11-11 2018-05-17 株式会社ニューフレアテクノロジー マルチ荷電粒子ビーム描画装置

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070041901A1 (en) * 2002-06-18 2007-02-22 Diener John L Stabilized aptamers to PSMA and their use as prostate cancer therapeutics

Also Published As

Publication number Publication date
EP2626436A2 (en) 2013-08-14
EP2489743B1 (en) 2015-01-07
JP5684568B2 (ja) 2015-03-11
EP2933340B1 (en) 2017-09-06
CN101809167B (zh) 2015-04-01
KR20100058485A (ko) 2010-06-03
JP2015062416A (ja) 2015-04-09
HK1174366A1 (en) 2013-06-07
PT2489743E (pt) 2015-04-09
MX375114B (es) 2025-03-06
CA2693453A1 (en) 2009-01-22
DK2172566T3 (en) 2015-05-04
HK1132012A1 (zh) 2010-02-12
US8409795B2 (en) 2013-04-02
WO2009012418A3 (en) 2009-03-05
MX2010000533A (es) 2010-04-30
CA2693448A1 (en) 2009-01-22
EP2172566B1 (en) 2015-03-04
JP2010533499A (ja) 2010-10-28
CA2693453C (en) 2018-12-11
EP2069529B1 (en) 2013-01-09
KR101656240B1 (ko) 2016-09-09
AU2008275917A1 (en) 2009-01-22
MX341491B (es) 2016-08-23
JP2020078311A (ja) 2020-05-28
CN107090457A (zh) 2017-08-25
JP2014050398A (ja) 2014-03-20
EP2069496A4 (en) 2010-01-27
HK1133046A1 (en) 2010-03-12
PL2489743T3 (pl) 2015-06-30
ES2647587T3 (es) 2017-12-22
ES2537322T3 (es) 2015-06-05
JP5404620B2 (ja) 2014-02-05
JP2023116788A (ja) 2023-08-22
EP2626436A3 (en) 2013-10-16
MX2010000578A (es) 2010-04-30
EP2336314A1 (en) 2011-06-22
AU2008276001A1 (en) 2009-01-22
AU2008276001A9 (en) 2010-06-10
EP2076613A4 (en) 2012-11-28
DK2069529T3 (da) 2013-04-15
JP2010533872A (ja) 2010-10-28
KR101634608B1 (ko) 2016-06-29
EP2076613B1 (en) 2016-09-07
JP7755619B2 (ja) 2025-10-16
WO2009012420A1 (en) 2009-01-22
US20090098549A1 (en) 2009-04-16
EP2069496A1 (en) 2009-06-17
JP5938080B2 (ja) 2016-06-22
JP6407195B2 (ja) 2018-10-17
AU2008275915A1 (en) 2009-01-22
CA3022666C (en) 2022-04-19
CN104593373A (zh) 2015-05-06
CN101809167A (zh) 2010-08-18
WO2009012410A1 (en) 2009-01-22
EP4056711A1 (en) 2022-09-14
MX355017B (es) 2018-04-02
ES2604764T3 (es) 2017-03-09
WO2009012418A2 (en) 2009-01-22
EP4056711C0 (en) 2025-09-03
JP2016122014A (ja) 2016-07-07
EP2069529A2 (en) 2009-06-17
EP2069529A4 (en) 2009-12-30
JP7303244B2 (ja) 2023-07-04
KR20160108584A (ko) 2016-09-19
EP2489743A2 (en) 2012-08-22
DK2489743T3 (en) 2015-03-02
CA2696431C (en) 2021-01-05
JP2019004901A (ja) 2019-01-17
EP3284832A3 (en) 2018-03-07
FI3284832T3 (fi) 2023-08-01
EP2933340A1 (en) 2015-10-21
JP2021129577A (ja) 2021-09-09
DK3284832T5 (da) 2024-10-07
AU2008275917B2 (en) 2014-08-21
CN118064441A (zh) 2024-05-24
EP2076613A1 (en) 2009-07-08
ES3045958T3 (en) 2025-12-01
JP2016171810A (ja) 2016-09-29
SI2489743T1 (sl) 2015-04-30
EP2172566B2 (en) 2022-05-18
JP2019039929A (ja) 2019-03-14
JP5956404B2 (ja) 2016-07-27
CA3022666A1 (en) 2009-01-22
DK3284832T3 (da) 2023-07-31
ES2533711T3 (es) 2015-04-14
HK1213020A1 (en) 2016-06-24
HK1246368A1 (en) 2018-09-07
HK1141839A1 (en) 2010-11-19
KR101747665B1 (ko) 2017-06-15
DK2172566T4 (da) 2022-06-13
AU2008275915A2 (en) 2010-04-22
PT2172566E (pt) 2015-06-23
ES2537322T5 (es) 2022-09-12
EP3284832B1 (en) 2023-06-07
JP6491610B2 (ja) 2019-03-27
CN101802225A (zh) 2010-08-11
NO2933340T3 (enExample) 2018-02-03
EP3284832A2 (en) 2018-02-21
MX344253B (es) 2016-12-09
MX2020009595A (es) 2020-10-07
KR20100044787A (ko) 2010-04-30
CN101802225B (zh) 2013-10-30
HRP20150382T1 (hr) 2015-05-08
ES2951068T3 (es) 2023-10-17
CN103627792A (zh) 2014-03-12
EP2172566A1 (en) 2010-04-07
AU2008275917A2 (en) 2010-04-29
EP4056711B1 (en) 2025-09-03
AU2008276001A2 (en) 2010-06-03
CA2696431A1 (en) 2009-01-22
EP2489743A3 (en) 2012-11-21

Similar Documents

Publication Publication Date Title
US11111495B2 (en) Method for generating aptamers with improved off-rates
AU2008275915B2 (en) Method for generating aptamers with improved off-rates
US8404830B2 (en) Method for generating aptamers with improved off-rates
AU2019201066B2 (en) Method for generating aptamers with improved off-rates
HK40077705A (en) Method for generating aptamers with improved off-rates
HK1246368B (en) Aptamer with improved rate of dissociation, and non-covalent aptamer-target complex thereof
HK1213020B (en) Aptamers with uridines and/or thymidines substituted at the 5-position with a benzyl group
HK1133046B (en) Method for generating aptamers with improved off-rates
HK1174366B (en) Aptamers with 5-(n-naphthyl)-substituted uridines

Legal Events

Date Code Title Description
DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 16 MAR 2010 AND 6 APR 2010

FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: SOMALOGIC OPERATING CO., INC.

Free format text: FORMER OWNER(S): SOMALOGIC, INC.