KR100566606B1 - 고순도알부민제조방법 - Google Patents
고순도알부민제조방법 Download PDFInfo
- Publication number
- KR100566606B1 KR100566606B1 KR1019970708469A KR19970708469A KR100566606B1 KR 100566606 B1 KR100566606 B1 KR 100566606B1 KR 1019970708469 A KR1019970708469 A KR 1019970708469A KR 19970708469 A KR19970708469 A KR 19970708469A KR 100566606 B1 KR100566606 B1 KR 100566606B1
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- KR
- South Korea
- Prior art keywords
- albumin
- solution
- cation exchange
- substrate
- chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 235000011152 sodium sulphate Nutrition 0.000 description 1
- UQSHZBSQKMVQBS-UHFFFAOYSA-M sodium;2-acetamido-3-(1h-indol-3-yl)propanoate Chemical compound [Na+].C1=CC=C2C(CC(NC(=O)C)C([O-])=O)=CNC2=C1 UQSHZBSQKMVQBS-UHFFFAOYSA-M 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000011146 sterile filtration Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 150000003457 sulfones Chemical class 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000000979 synthetic dye Substances 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000000825 ultraviolet detection Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Immunology (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Neurology (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Dermatology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US378859 | 1995-05-25 | ||
| US08/378,859 US5728553A (en) | 1992-09-23 | 1995-05-25 | High purity albumin and method of producing |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020047011825A Division KR100543612B1 (ko) | 1995-05-25 | 1996-02-29 | 고 순도 알부민 제조방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR19990021994A KR19990021994A (ko) | 1999-03-25 |
| KR100566606B1 true KR100566606B1 (ko) | 2006-12-01 |
Family
ID=23494845
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019970708469A Expired - Lifetime KR100566606B1 (ko) | 1995-05-25 | 1996-02-29 | 고순도알부민제조방법 |
| KR1020047011825A Expired - Lifetime KR100543612B1 (ko) | 1995-05-25 | 1996-02-29 | 고 순도 알부민 제조방법 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020047011825A Expired - Lifetime KR100543612B1 (ko) | 1995-05-25 | 1996-02-29 | 고 순도 알부민 제조방법 |
Country Status (15)
| Country | Link |
|---|---|
| US (6) | US5728553A (enExample) |
| EP (2) | EP1031578B1 (enExample) |
| JP (4) | JP4040088B2 (enExample) |
| KR (2) | KR100566606B1 (enExample) |
| CN (4) | CN100584857C (enExample) |
| AT (2) | ATE198482T1 (enExample) |
| AU (1) | AU698409B2 (enExample) |
| CA (1) | CA2220923C (enExample) |
| DE (2) | DE69611445T2 (enExample) |
| DK (2) | DK1031578T3 (enExample) |
| ES (2) | ES2156991T3 (enExample) |
| GR (1) | GR3035651T3 (enExample) |
| MX (1) | MX9709108A (enExample) |
| PT (2) | PT1031578E (enExample) |
| WO (1) | WO1996037515A1 (enExample) |
Families Citing this family (150)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2686899B1 (fr) * | 1992-01-31 | 1995-09-01 | Rhone Poulenc Rorer Sa | Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant. |
| US5728553A (en) * | 1992-09-23 | 1998-03-17 | Delta Biotechnology Limited | High purity albumin and method of producing |
| DE19538625C2 (de) * | 1995-10-17 | 1999-08-12 | Sartorius Gmbh | Verfahren zur Abtrennung von Albumin aus Serum durch Membranionenaustauschchromatographie |
| GB9526733D0 (en) * | 1995-12-30 | 1996-02-28 | Delta Biotechnology Ltd | Fusion proteins |
| ATE241695T1 (de) * | 1996-03-13 | 2003-06-15 | Delta Biotechnology Ltd | Gärungs-steuerungssysteme |
| GB9902000D0 (en) | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
| AU2007200859A1 (en) * | 1999-01-30 | 2007-03-22 | Novozymes Delta Limited | Process |
| FR2792650B1 (fr) * | 1999-04-20 | 2003-02-28 | Oreal | Equivalent de peau agee, son procede de preparation et son utilisation |
| US20030118598A1 (en) * | 2000-02-08 | 2003-06-26 | Allergan, Inc. | Clostridial toxin pharmaceutical compositions |
| US8632785B2 (en) | 2000-02-08 | 2014-01-21 | Allergan, Inc. | Clostridial toxin pharmaceutical composition containing a gelatin fragment |
| US20060269575A1 (en) * | 2000-02-08 | 2006-11-30 | Allergan, Inc. | Botulinum toxin pharmaceutical compositions formulated with recombinant albumin |
| US7780967B2 (en) | 2000-02-08 | 2010-08-24 | Allergan, Inc. | Reduced toxicity Clostridial toxin pharmaceutical compositions |
| CA2494241C (en) * | 2000-02-08 | 2011-06-14 | Allergan, Inc. | Botulinum toxin pharmaceutical compositions |
| US20050100991A1 (en) * | 2001-04-12 | 2005-05-12 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| CA2405550A1 (en) | 2000-04-12 | 2001-10-25 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| DK1311269T3 (da) | 2000-08-04 | 2012-03-26 | Dmi Biosciences Inc | Fremgangsmåde til anvendelse af diketopiperaziner og sammensætning, som indeholder dem |
| JP4798833B2 (ja) | 2000-10-24 | 2011-10-19 | 一般財団法人化学及血清療法研究所 | 加熱処理工程を含むヒト血清アルブミンの製造方法 |
| CN1275981C (zh) * | 2000-10-24 | 2006-09-20 | 财团法人化学及血清疗法研究所 | 人血清白蛋白多聚体的单体化方法 |
| JP4798832B2 (ja) * | 2000-10-24 | 2011-10-19 | 一般財団法人化学及血清療法研究所 | ヒト血清アルブミン多量体の除去方法 |
| US7507413B2 (en) * | 2001-04-12 | 2009-03-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| US20050054051A1 (en) * | 2001-04-12 | 2005-03-10 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| CA2448432A1 (en) * | 2001-06-13 | 2002-12-19 | Taurus Hsa Llc | Purification of human serum albumin |
| ITBO20010426A1 (it) * | 2001-07-06 | 2003-01-06 | Alfa Wassermann Spa | Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico |
| JP2003040798A (ja) * | 2001-07-26 | 2003-02-13 | Nihon Pharmaceutical Co Ltd | 低アルミニウム含有アルブミン製剤およびその製造法 |
| US7176278B2 (en) | 2001-08-30 | 2007-02-13 | Biorexis Technology, Inc. | Modified transferrin fusion proteins |
| CA2484556A1 (en) * | 2001-12-21 | 2003-07-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| EP1465869B1 (en) * | 2001-12-21 | 2013-05-15 | Exelixis Patent Company LLC | Modulators of lxr |
| US7482366B2 (en) | 2001-12-21 | 2009-01-27 | X-Ceptor Therapeutics, Inc. | Modulators of LXR |
| US20080167238A1 (en) * | 2001-12-21 | 2008-07-10 | Human Genome Sciences, Inc. | Albumin Fusion Proteins |
| AU2002364586A1 (en) | 2001-12-21 | 2003-07-30 | Delta Biotechnology Limited | Albumin fusion proteins |
| GB0217033D0 (en) | 2002-07-23 | 2002-08-28 | Delta Biotechnology Ltd | Gene and polypeptide sequences |
| EP1571970B1 (en) * | 2002-10-02 | 2011-08-17 | DMI Biosciences, Inc. | Diagnosis and monitoring of diseases |
| CA2513213C (en) | 2003-01-22 | 2013-07-30 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| ES2572975T3 (es) | 2003-05-15 | 2016-06-03 | Ampio Pharmaceuticals, Inc. | Tratamiento de enfermedades mediadas por los linfocitos T |
| US7339347B2 (en) * | 2003-08-11 | 2008-03-04 | Reserve Power Cell, Llc | Apparatus and method for reliably supplying electrical energy to an electrical system |
| US8013611B2 (en) * | 2006-07-14 | 2011-09-06 | Reserve Power Cell, Llc | Vehicle battery product and battery monitoring system |
| GB0329722D0 (en) * | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Modified plasmid and use thereof |
| GB0329681D0 (en) | 2003-12-23 | 2004-01-28 | Delta Biotechnology Ltd | Gene expression technique |
| AU2004313242A1 (en) * | 2004-01-07 | 2005-07-28 | Trimeris, Inc. | HIV gp41 HR2-derived synthetic peptides, and their use in therapy to inhibit transmission of human immunodeficiency virus |
| AU2005205314B2 (en) | 2004-01-20 | 2010-12-09 | Km Biologics Co., Ltd. | Process for producing human serum albumin by heating in presence of divalent cation |
| US20060182783A1 (en) * | 2004-04-30 | 2006-08-17 | Allergan, Inc. | Sustained release intraocular drug delivery systems |
| WO2005110374A1 (en) * | 2004-04-30 | 2005-11-24 | Allergan, Inc. | Intraocular drug delivery systems containing a therapeutic component, a cyclodextrin, and a polymeric component |
| US20070059336A1 (en) * | 2004-04-30 | 2007-03-15 | Allergan, Inc. | Anti-angiogenic sustained release intraocular implants and related methods |
| US8591885B2 (en) * | 2004-04-30 | 2013-11-26 | Allergan, Inc. | Carbonic anhydrase inhibitor sustained release intraocular drug delivery systems |
| JP5631533B2 (ja) * | 2004-12-23 | 2014-11-26 | ノボザイムス バイオファーマ デーコー アクティーゼルスカブ | 遺伝子発現技術 |
| CA2599731A1 (en) * | 2005-03-03 | 2006-09-08 | Dmi Biosciences, Inc. | Quantification of proteins |
| US7598700B2 (en) * | 2005-03-30 | 2009-10-06 | Reserve Power Cell, Llc | Tamper resistant battery and battery warranty and performance tracking system |
| MY144344A (en) * | 2005-04-08 | 2011-09-15 | Ciba Holding Inc | Adsorbents comprising anthraquinone dye-ligands for the separation of biological materials |
| DE102005023155A1 (de) | 2005-05-13 | 2006-11-16 | Albutec Gmbh | Albuminlösung |
| US8323666B2 (en) | 2005-08-01 | 2012-12-04 | Allergan, Inc. | Botulinum toxin compositions |
| GB0518460D0 (en) * | 2005-09-09 | 2005-10-19 | Univ Nottingham Trent | Protein assay |
| DK1989220T3 (da) | 2006-02-02 | 2012-04-02 | Trimeris Inc | HIV-fusionsinhibitorpeptider med forbedrede biologiske egenskaber |
| JP2007284425A (ja) * | 2006-03-20 | 2007-11-01 | Nokodai Tlo Kk | タンパク質の精製方法 |
| EP2046367A4 (en) * | 2006-06-07 | 2009-11-11 | Human Genome Sciences Inc | ALBUM INFUSION PROTEINS |
| US9139611B2 (en) | 2006-07-13 | 2015-09-22 | Novozymes Biopharma Dk A/S | Process for preparing particles of proteinaceous material |
| ES2371495T3 (es) | 2006-07-24 | 2012-01-03 | Biorexis Pharmaceutical Corporation | Proteínas de fusión de exendina. |
| US10829733B2 (en) * | 2007-01-04 | 2020-11-10 | Biolamina Ab | Composition and method for enabling proliferation of pluripotent human stem cells |
| JP2010523564A (ja) * | 2007-04-03 | 2010-07-15 | トリメリス,インコーポレーテッド | 抗ウイルス性ペプチド治療薬の送達のための新規製剤 |
| CN101104635B (zh) * | 2007-04-30 | 2010-11-03 | 北京济普霖生物技术有限公司 | 一种从转基因牛乳中纯化重组人α-乳清白蛋白的方法 |
| WO2009019314A1 (en) | 2007-08-08 | 2009-02-12 | Novozymes A/S | Transferrin variants and conjugates |
| CN101874038A (zh) * | 2007-09-25 | 2010-10-27 | 特里梅里斯公司 | 治疗性抗-hiv肽的合成方法 |
| JP4452324B2 (ja) | 2008-03-31 | 2010-04-21 | 積水メディカル株式会社 | 精製血清アルブミン及び免疫学的測定方法 |
| NZ602479A (en) * | 2008-05-15 | 2013-05-31 | R Tech Ueno Ltd | Pharmaceutical composition for the treatment of dry eye and/or corneal and conjunctival lesion |
| WO2009146320A1 (en) | 2008-05-27 | 2009-12-03 | Dmi Life Sciences, Inc. | Therapeutic methods and compounds |
| EP2313430B1 (en) | 2008-06-24 | 2018-05-02 | Technische Universität München | Muteins of hngal and related proteins with affinity for a given target |
| KR101722961B1 (ko) | 2009-02-11 | 2017-04-04 | 알부메딕스 에이/에스 | 알부민 변이체 및 접합체 |
| SG173469A1 (en) | 2009-02-20 | 2011-09-29 | Ventria Bioscience | Cell culture media containing combinations of proteins |
| DE102009013914B4 (de) * | 2009-03-19 | 2011-05-05 | Bruker Daltonik Gmbh | Kalibriersubstanzen für Atmosphärendruck-Ionenquellen |
| CN102549012B (zh) * | 2009-05-07 | 2015-07-01 | 诺维信生物制药丹麦公司 | 纯化白蛋白的方法 |
| CN102612362A (zh) | 2009-08-05 | 2012-07-25 | 皮里斯股份公司 | 脂质运载蛋白突变蛋白的控制释放制剂 |
| SG10201408106WA (en) * | 2009-08-20 | 2015-01-29 | Baxter Int | Purification of vwf for increased removal of non-lipid enveloped viruses |
| MX2012004793A (es) | 2009-10-30 | 2012-07-20 | Novozymes Biopharma Dk As | Variantes de albumina. |
| US9549968B2 (en) | 2009-12-07 | 2017-01-24 | Pieris Pharmaceuticals Gmbh | Muteins of human lipocalin 2 (LcnC,hNGAL) with affinity for a given target |
| SG182618A1 (en) * | 2010-01-25 | 2012-08-30 | Ventria Bioscience | Methods & compositions for improving protein production |
| JP5969458B2 (ja) | 2010-04-09 | 2016-08-17 | アルブミディクス アクティーゼルスカブ | アルブミン誘導体及び変異体 |
| SMT201900297T1 (it) | 2010-06-08 | 2019-07-11 | Astrazeneca Ab | Muteine di lipocalina lacrimale che si legano a il-4 r alfa |
| CA2808392C (en) | 2010-08-16 | 2020-03-10 | Pieris Ag | Binding proteins for hepcidin |
| US8507496B2 (en) | 2010-09-07 | 2013-08-13 | Dmi Acquisition Corp. | Treatment of diseases |
| DK2640740T3 (en) | 2010-11-15 | 2017-06-26 | Pieris Pharmaceuticals Gmbh | MUTEINS OF HUMAN LIPOCALIN 2 WITH AFFINITY FOR GLYPICAN-3 (GPC3) |
| US9221885B2 (en) | 2010-12-02 | 2015-12-29 | Pieris Ag | Muteins of human lipocalin 2 with affinity for CTLA-4 |
| PL221899B1 (pl) | 2011-05-06 | 2016-06-30 | Wrocławskie Centrum Badań Eit + Spółka Z Ograniczoną | Czysta albumina oraz sposób jej otrzymywania i detekcji |
| CN103649111B (zh) | 2011-07-05 | 2018-03-13 | 阿尔布梅迪克斯医疗公司 | 白蛋白制剂和用途 |
| BR112014007675A2 (pt) | 2011-10-10 | 2017-04-18 | Ampio Pharmaceuticals Inc | tratamento de doença articular degenerativa |
| BR112014007657A2 (pt) | 2011-10-10 | 2017-04-11 | Ampio Pharmaceuticals Inc | dispositivos médicos implantáveis com tolerância imune aperfeiçoada e métodos para produção e implantação |
| WO2013063413A1 (en) | 2011-10-28 | 2013-05-02 | Ampio Pharmaceuticals, Inc. | Treatment of rhinitis |
| US20140315817A1 (en) | 2011-11-18 | 2014-10-23 | Eleven Biotherapeutics, Inc. | Variant serum albumin with improved half-life and other properties |
| SG10201604566QA (en) | 2011-12-13 | 2016-07-28 | Pieris Ag | Methods for preventing or treating certain disorders by inhibiting binding of il-4 and/or il-13 to their respective receptors |
| DK2825556T3 (en) | 2012-03-16 | 2018-04-16 | Albumedix As | albumin Variants |
| US9522940B2 (en) | 2012-05-23 | 2016-12-20 | Pieris Pharmaceuticals Gmbh | Lipocalin muteins with binding-affinity for glypican-3 (GPC-3) and use of lipocalin muteins for target-specific delivery to cells expressing GPC-3 |
| US20140031527A1 (en) * | 2012-07-27 | 2014-01-30 | Csl Limited | Method for polishing albumin |
| KR20150082422A (ko) | 2012-11-08 | 2015-07-15 | 노보자임스 바이오파마 디케이 에이/에스 | 알부민 변이체 |
| US20140186447A1 (en) * | 2012-12-28 | 2014-07-03 | Abraxis Bioscience, Llc | Nanoparticle compositions of albumin and paclitaxel |
| JP6128677B2 (ja) * | 2013-02-05 | 2017-05-17 | 公立大学法人大阪市立大学 | 神経細胞の遊走障害を伴う疾患の判定方法及びその利用 |
| CA2905186A1 (en) | 2013-03-14 | 2014-09-18 | Daiichi Sankyo Co., Ltd | Novel binding proteins for pcsk9 |
| US9808454B2 (en) | 2013-03-15 | 2017-11-07 | Ampio Pharmaceuticals, Inc. | Compositions for the mobilization, homing, expansion and differentiation of stem cells and methods of using the same |
| CA2891557A1 (en) | 2013-03-26 | 2014-05-22 | Pieris Ag | Novel specific-binding polypeptides and uses thereof |
| CN103694343B (zh) * | 2013-12-26 | 2016-05-25 | 扬州艾迪生物科技有限公司 | 一种从尿液中制备人血白蛋白的方法 |
| AU2015205530B8 (en) | 2014-01-13 | 2019-09-19 | Pieris Pharmaceuticals Gmbh | Multi-specific polypeptide useful for localized tumor immunomodulation |
| DE22197320T1 (de) * | 2014-04-30 | 2024-05-29 | Novo-Nordisk A/S | Verfahren zur reinigung von proteinen mittels caprylsäure |
| CN113621050A (zh) | 2014-05-22 | 2021-11-09 | 皮里斯制药有限公司 | 新型特异性结合多肽及其用途 |
| EP3183240A4 (en) | 2014-08-18 | 2018-06-27 | Ampio Pharmaceuticals, Inc. | Treatment of joint conditions |
| CN104628830A (zh) * | 2015-01-23 | 2015-05-20 | 深圳康泰生物制品股份有限公司 | 重组酿酒酵母表达HBsAg的制备工艺及其分离纯化工艺、HBsAg和乙肝疫苗 |
| SG10201906859PA (en) | 2015-01-28 | 2019-08-27 | Pieris Pharmaceuticals Gmbh | Novel proteins specific for angiogenesis |
| AR103714A1 (es) | 2015-02-18 | 2017-05-31 | Sanofi Sa | Proteínas específicas para pioverdina y pioquelina |
| US10705070B1 (en) | 2015-03-05 | 2020-07-07 | Abraxis Bioscience, Llc | Methods of assessing suitability of use of pharmaceutical compositions of albumin and poorly water soluble drug |
| US10527604B1 (en) | 2015-03-05 | 2020-01-07 | Abraxis Bioscience, Llc | Methods of assessing suitability of use of pharmaceutical compositions of albumin and paclitaxel |
| US11261221B2 (en) | 2015-05-04 | 2022-03-01 | Pieris Pharmaceuticals Gmbh | Proteins specific for CD137 |
| CN107636014B (zh) | 2015-05-04 | 2021-12-07 | 皮里斯制药有限公司 | 抗癌融合多肽 |
| CN107787327B (zh) | 2015-05-18 | 2022-02-08 | 皮里斯制药有限公司 | 对磷脂酰肌醇聚糖-3(gpc3)具有亲和力的人脂质运载蛋白2的突变蛋白 |
| FI3298030T3 (fi) | 2015-05-18 | 2023-02-22 | Pieris Pharmaceuticals Gmbh | Syöpää ehkäisevä fuusiopolypeptidi |
| HK1246669A1 (zh) | 2015-06-22 | 2018-09-14 | Ampio Pharmaceuticals, Inc. | 人血清白蛋白低分子量组分在疾病治疗中的应用 |
| EP3115371A1 (en) | 2015-07-07 | 2017-01-11 | Sanofi | Fusion molecules |
| CN108348573A (zh) | 2015-07-15 | 2018-07-31 | 皮里斯制药有限公司 | Lag-3特异性的新型蛋白 |
| EP3337816B1 (en) | 2015-08-20 | 2024-02-14 | Albumedix Ltd | Albumin variants and conjugates |
| FR3040882A1 (fr) * | 2015-09-10 | 2017-03-17 | Lab Francais Du Fractionnement | Composition liquide d'albumine humaine a usage therapeutique |
| SG11201803732PA (en) | 2015-11-30 | 2018-06-28 | Pieris Australia Pty Ltd | Novel anti-angiogenic fusion polypeptides |
| TW201725212A (zh) | 2015-12-10 | 2017-07-16 | 第一三共股份有限公司 | 特異性於降鈣素基因相關胜肽的新穎蛋白 |
| CA3005953A1 (en) | 2015-12-22 | 2017-06-29 | Albumedix Ltd | Improved protein expression strains |
| JP7146637B2 (ja) | 2015-12-22 | 2022-10-04 | アルブミディクス リミティド | 改善されたタンパク質発現株 |
| KR102050852B1 (ko) * | 2016-03-11 | 2019-12-03 | 주식회사 스템온 | 세포 리프로그래밍 장치 |
| CN118085011A (zh) * | 2016-08-17 | 2024-05-28 | 勃林格殷格翰国际公司 | 制备含有生物分子的高度浓缩的液体制剂的方法 |
| EP3429345B1 (en) | 2016-10-04 | 2019-09-11 | Albumedix Ltd. | Uses of recombinant yeast-derived serum albumin |
| WO2018087108A1 (en) | 2016-11-09 | 2018-05-17 | Pieris Pharmaceuticals Gmbh | Proteins specific for cd137 |
| MX2019008434A (es) | 2017-01-18 | 2019-11-11 | Pieris Pharmaceuticals Gmbh | Muteínas de lipocalina con afinidad de unión por lag-3. |
| WO2018165766A1 (en) * | 2017-03-16 | 2018-09-20 | Therapure Biopharma Inc. | Method for purification of albumin |
| US11130979B2 (en) | 2017-06-20 | 2021-09-28 | Albumedix Ltd | Protein expression strains |
| WO2019199476A1 (en) * | 2018-04-12 | 2019-10-17 | Amgen Inc. | Methods for making stable protein compositions |
| CN110563833A (zh) * | 2018-06-06 | 2019-12-13 | 中国计量科学研究院 | 一种人血白蛋白标准物质原料的制备方法及其产品和应用 |
| AU2019315703A1 (en) | 2018-07-31 | 2020-12-10 | Les Laboratoires Servier | Novel fusion protein specific for CD137 and PD-L1 |
| CN109734796B (zh) * | 2019-02-01 | 2022-04-15 | 广州蕊特生物科技有限公司 | 一种从溶血血清中分离白蛋白的工艺 |
| CN109776673B (zh) * | 2019-02-20 | 2022-03-29 | 天津理工大学 | 一种适合中试量产的高纯度牛血清白蛋白纯化工艺 |
| US12486330B2 (en) | 2019-02-26 | 2025-12-02 | Pieris Pharmaceuticals Gmbh | Fusion proteins specific for CD137 and GPC3 |
| CN110229229A (zh) * | 2019-07-10 | 2019-09-13 | 甘肃养泰和生物科技有限公司 | 一种从牛血浆中提取纯化细胞培养级牛血清白蛋白的生产方法及其应用 |
| EP4051241A4 (en) | 2019-10-28 | 2023-12-06 | Abraxis BioScience, LLC | PHARMACEUTICAL COMPOSITIONS OF ALBUMIN AND RAPAMYCIN |
| CN116249709A (zh) | 2020-06-05 | 2023-06-09 | 皮里斯制药有限公司 | 靶向4-1bb的多聚体免疫调节剂 |
| US12161777B2 (en) | 2020-07-02 | 2024-12-10 | Davol Inc. | Flowable hemostatic suspension |
| US11739166B2 (en) | 2020-07-02 | 2023-08-29 | Davol Inc. | Reactive polysaccharide-based hemostatic agent |
| IL300549A (en) * | 2020-08-11 | 2023-04-01 | Shenzhen Protgen Ltd | Young unblemished human serum albumin that improves human life expectancy |
| WO2022089639A1 (zh) | 2020-10-30 | 2022-05-05 | 深圳普罗吉医药科技有限公司 | 人血清白蛋白在治疗疾病中的应用 |
| CN114885608A (zh) | 2020-12-08 | 2022-08-09 | 通化安睿特生物制药股份有限公司 | 纯化重组蛋白的方法 |
| CN112592399B (zh) * | 2020-12-15 | 2024-04-12 | 甘肃养泰和生物科技有限公司 | 一种从牛血清中分离牛血清白蛋白的制备方法及装置 |
| CN116744984A (zh) | 2020-12-28 | 2023-09-12 | 达沃有限公司 | 包含蛋白质和多官能化改性的基于聚乙二醇的交联剂的反应性干粉状止血材料 |
| EP4320141A1 (en) | 2021-04-08 | 2024-02-14 | Pieris Pharmaceuticals GmbH | Novel lipocalin muteins specific for connective tissue growth factor (ctgf) |
| WO2022243341A1 (en) | 2021-05-18 | 2022-11-24 | Pieris Pharmaceuticals Gmbh | Lipocalin muteins with binding affinity for ox40 |
| CN113735963A (zh) * | 2021-09-10 | 2021-12-03 | 山东健通生物科技有限公司 | 一种重组人血清白蛋白纯化过程去除色素的方法 |
| CN114316088B (zh) * | 2021-12-22 | 2022-09-09 | 中国水产科学研究院黄海水产研究所 | 亲和树脂、制备方法及其在分离纯化藻蓝蛋白中的应用 |
| TW202428603A (zh) | 2022-09-21 | 2024-07-16 | 美商思進公司 | 新穎的cd137及cd228特異性融合蛋白 |
| CN115677848A (zh) * | 2022-10-24 | 2023-02-03 | 通化安睿特生物制药股份有限公司 | 一种制备高纯度高稳定性蛋白质的方法 |
| CN116120392B (zh) * | 2023-04-18 | 2023-08-01 | 上海健士拜生物科技有限公司 | 聚体蛋白的纯化方法 |
| WO2025175123A1 (en) | 2024-02-16 | 2025-08-21 | Seagen Inc. | Methods of treating cancer using fusion proteins specific for cd137 and cd228 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992004367A1 (en) * | 1990-09-12 | 1992-03-19 | Delta Biotechnology Limited | Separation of proteins and dyes |
| EP0570916A2 (en) * | 1992-05-20 | 1993-11-24 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
Family Cites Families (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2433905A (en) | 1943-11-01 | 1948-01-06 | Jr Walter L Hughes | Method for the crystallization of albumin and the preparation of protein products therefrom |
| AT317418B (de) * | 1972-06-16 | 1974-08-25 | Brigitte Panhofer | Verfahren zur Herstellung von intravenös beim Menschen anwendbarem Humanalbumin aus menschlichen Placenten |
| FR2190437B1 (enExample) | 1972-07-05 | 1975-08-08 | Merieux Inst | |
| CA1044537A (en) | 1973-05-09 | 1978-12-19 | Amf Incorporated | Filter medium and process |
| DE2537123A1 (de) * | 1975-08-20 | 1977-03-03 | New Zealand Inventions Dev | Verfahren zur herstellung von albumin |
| SE405549B (sv) * | 1975-10-09 | 1978-12-18 | Pharmacia Fine Chemicals Ab | Forfarande for isolering av albumin ur plasmaprodukter genom kromatografisk fraktionering |
| US4043997A (en) * | 1976-05-28 | 1977-08-23 | Cutter Laboratories, Inc. | Method for isolating albumin using insoluble supports coupled to a dye |
| US4075197A (en) * | 1976-09-20 | 1978-02-21 | Monsanto Company | Serum albumin production |
| IN150740B (enExample) | 1978-11-24 | 1982-12-04 | Hoffmann La Roche | |
| US4228154A (en) * | 1979-02-26 | 1980-10-14 | Armour Pharmaceutical Company | Purification of plasma albumin by ion exchange chromatography |
| US4222934A (en) * | 1979-04-12 | 1980-09-16 | American National Red Cross | Preparation of albumin using ethanol |
| GB2053926B (en) | 1979-07-20 | 1983-02-23 | Atkinson A | Albumin extraction by affinity chromatography |
| US4350156A (en) * | 1980-05-29 | 1982-09-21 | Japan Foundation For Artificial Organs | Method and apparatus for on-line filtration removal of macromolecules from a physiological fluid |
| IL66614A (en) * | 1981-08-28 | 1985-09-29 | Genentech Inc | Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it |
| US4391801A (en) | 1981-10-29 | 1983-07-05 | Cutter Laboratories, Inc. | Plasma protein fraction substantially free of acetate ions |
| FR2543448A1 (fr) * | 1983-04-01 | 1984-10-05 | Rhone Poulenc Spec Chim | Procede de fractionnement du plasma |
| US4748120A (en) * | 1983-05-02 | 1988-05-31 | Diamond Scientific Co. | Photochemical decontamination treatment of whole blood or blood components |
| DE3344656A1 (de) * | 1983-12-09 | 1985-06-13 | Lentia GmbH Chem. u. pharm. Erzeugnisse - Industriebedarf, 8000 München | Verfahren zur herstellung einer serumproteinloesung |
| CA1286622C (en) * | 1986-04-28 | 1991-07-23 | Chokyun Rha | Method for clarifying and stabilizing cell culture media |
| JPS62294621A (ja) | 1986-06-13 | 1987-12-22 | Green Cross Corp:The | アルブミンの回収方法 |
| JPS6383100A (ja) * | 1986-09-26 | 1988-04-13 | Green Cross Corp:The | ヒト尿中アルブミンの製造方法 |
| US4833233A (en) * | 1987-08-20 | 1989-05-23 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Human serum albumin crystals and method of preparation |
| IL88326A (en) | 1987-11-18 | 1993-03-15 | Gist Brocades Nv | Purification of serum albumin |
| US4990447A (en) * | 1988-06-24 | 1991-02-05 | Gist-Brocades Nv | Process for the purification of serum albumin |
| FR2649991B2 (fr) | 1988-08-05 | 1994-03-04 | Rhone Poulenc Sante | Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces |
| JP3554796B2 (ja) * | 1988-10-31 | 2004-08-18 | 三菱ウェルファーマ株式会社 | アルブミン製剤及びその製造方法 |
| US4891221A (en) * | 1988-11-23 | 1990-01-02 | Edward Shanborm | Whole blood antiviral process and composition |
| FR2648048B1 (fr) * | 1989-06-08 | 1994-06-03 | Lille Transfusion Sanguine | Procede de preparation de solutions d'albumine purifiee |
| GB8913586D0 (en) | 1989-06-13 | 1989-08-02 | Technicol Limited | A method of determining the glycosylated protein level of a sample and an apparatus for use in the method |
| JPH0768137B2 (ja) * | 1989-06-15 | 1995-07-26 | 株式会社ミドリ十字 | アルブミン製剤及びその製法 |
| SE500110C2 (sv) | 1989-06-27 | 1994-04-18 | Kabi Pharmacia Ab | Sätt att rena ett protein från därtill bundna flervärda metalljoner |
| JPH0671434B2 (ja) | 1989-09-18 | 1994-09-14 | 株式会社ミドリ十字 | ヒト血清アルブミンの製造方法 |
| US5250662A (en) * | 1989-10-05 | 1993-10-05 | Alpha Therapeutic Corporation | Albumin purification |
| CA2022165C (en) | 1989-10-05 | 1999-11-23 | Chong E. Chang | Albumin purification |
| ES2224094T3 (es) * | 1990-04-19 | 2005-03-01 | Bayer Corporation | Procedimiento para preparar seroalbumina normal (humana) esencialmente monomerica. |
| JP3230091B2 (ja) * | 1990-06-25 | 2001-11-19 | ウェルファイド株式会社 | ヒト血清アルブミンの着色抑制方法 |
| JP2982296B2 (ja) | 1990-11-19 | 1999-11-22 | 吉富製薬株式会社 | アルブミン含有水溶液の精製法 |
| JP2949846B2 (ja) * | 1990-11-30 | 1999-09-20 | 吉富製薬株式会社 | アルブミン製剤の保存方法 |
| FR2672604B1 (fr) | 1991-02-07 | 1995-05-05 | Pasteur Merieux Serums Vacc | Procede pour isoler de l'albumine humaine a partir du surnageant iv, notamment iv-4, ou de la fraction v de cohn ou d'un surnageant ou fraction analogue. |
| US5284777A (en) | 1991-03-04 | 1994-02-08 | Isolab, Inc. | Combined glycated hemoglobin and immunoturbidometric glycated albumin assay from whole blood lysate |
| US5330901A (en) * | 1991-04-26 | 1994-07-19 | Research Corporation Technologies, Inc. | Expression of human serum albumin in Pichia pastoris |
| EP0933083A1 (en) * | 1991-07-12 | 1999-08-04 | Dsm N.V. | Process for the purification of serum albumin |
| FR2686620B1 (fr) * | 1992-01-27 | 1995-06-23 | Rhone Poulenc Rorer Sa | Serum-albumine humaine, preparation et utilisation. |
| US5281582A (en) * | 1992-02-27 | 1994-01-25 | Alliance Pharmaceuticals, Corp. | Serum growth factor |
| JP3360315B2 (ja) * | 1992-07-31 | 2002-12-24 | 三菱ウェルファーマ株式会社 | ヒト血清アルブミンの高度精製方法 |
| DE4226971C2 (de) | 1992-08-14 | 1997-01-16 | Widmar Prof Dr Tanner | Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte |
| US5728553A (en) * | 1992-09-23 | 1998-03-17 | Delta Biotechnology Limited | High purity albumin and method of producing |
| CN1055095C (zh) * | 1992-12-11 | 2000-08-02 | 上海莱士血制品有限公司 | 白蛋白的纯化方法 |
| CA2116385A1 (en) * | 1993-02-25 | 1994-08-26 | Akinori Sumi | Human serum albumin and process for producing the same |
| SE9301583D0 (sv) | 1993-05-07 | 1993-05-07 | Kabi Pharmacia Ab | Yeast strain and methods for expressing heterologous proteins in yeast |
| JP3702474B2 (ja) | 1994-06-01 | 2005-10-05 | 三菱ウェルファーマ株式会社 | 血清アルブミン製剤の製造方法 |
| CA2157219C (en) * | 1994-08-31 | 2010-10-05 | Munehiro Noda | Process for purifying recombinant human serum albumin |
| JPH09209303A (ja) | 1996-02-05 | 1997-08-12 | Hokkaido Supuritsuton Kogyo Kk | 舗装ブロック |
| AU4837996A (en) | 1996-02-29 | 1997-09-16 | Delta Biotechnology Limited | High purity albumin production process |
-
1995
- 1995-05-25 US US08/378,859 patent/US5728553A/en not_active Expired - Lifetime
-
1996
- 1996-02-29 KR KR1019970708469A patent/KR100566606B1/ko not_active Expired - Lifetime
- 1996-02-29 WO PCT/GB1996/000449 patent/WO1996037515A1/en not_active Ceased
- 1996-02-29 EP EP00201960A patent/EP1031578B1/en not_active Expired - Lifetime
- 1996-02-29 DK DK00201960T patent/DK1031578T3/da active
- 1996-02-29 EP EP96904190A patent/EP0828759B1/en not_active Expired - Lifetime
- 1996-02-29 DE DE69611445T patent/DE69611445T2/de not_active Expired - Lifetime
- 1996-02-29 PT PT00201960T patent/PT1031578E/pt unknown
- 1996-02-29 CN CN03127743A patent/CN100584857C/zh not_active Expired - Lifetime
- 1996-02-29 AU AU48380/96A patent/AU698409B2/en not_active Ceased
- 1996-02-29 CN CNB2004100575838A patent/CN100455597C/zh not_active Expired - Lifetime
- 1996-02-29 DK DK96904190T patent/DK0828759T3/da active
- 1996-02-29 ES ES96904190T patent/ES2156991T3/es not_active Expired - Lifetime
- 1996-02-29 CN CNB961958243A patent/CN1198844C/zh not_active Expired - Lifetime
- 1996-02-29 JP JP53545796A patent/JP4040088B2/ja not_active Expired - Lifetime
- 1996-02-29 KR KR1020047011825A patent/KR100543612B1/ko not_active Expired - Lifetime
- 1996-02-29 CN CNB2006101006176A patent/CN100443499C/zh not_active Expired - Lifetime
- 1996-02-29 AT AT96904190T patent/ATE198482T1/de active
- 1996-02-29 ES ES00201960T patent/ES2219257T3/es not_active Expired - Lifetime
- 1996-02-29 US US08/952,558 patent/US6638740B1/en not_active Expired - Fee Related
- 1996-02-29 PT PT96904190T patent/PT828759E/pt unknown
- 1996-02-29 DE DE69632353T patent/DE69632353T2/de not_active Expired - Lifetime
- 1996-02-29 AT AT00201960T patent/ATE265469T1/de active
- 1996-02-29 CA CA002220923A patent/CA2220923C/en not_active Expired - Lifetime
-
1997
- 1997-11-14 US US08/970,648 patent/US6034221A/en not_active Expired - Lifetime
- 1997-11-25 MX MX9709108A patent/MX9709108A/es unknown
-
2001
- 2001-03-29 GR GR20010400501T patent/GR3035651T3/el unknown
-
2002
- 2002-05-31 JP JP2002160250A patent/JP4037174B2/ja not_active Expired - Lifetime
- 2002-11-21 US US10/301,357 patent/US20030187226A1/en not_active Abandoned
-
2004
- 2004-06-22 US US10/873,504 patent/US7223561B2/en not_active Expired - Fee Related
-
2006
- 2006-07-04 JP JP2006184756A patent/JP4372772B2/ja not_active Expired - Lifetime
-
2007
- 2007-04-23 US US11/738,582 patent/US7601515B2/en not_active Expired - Fee Related
- 2007-07-30 JP JP2007197742A patent/JP4372813B2/ja not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992004367A1 (en) * | 1990-09-12 | 1992-03-19 | Delta Biotechnology Limited | Separation of proteins and dyes |
| EP0570916A2 (en) * | 1992-05-20 | 1993-11-24 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
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