CN114885608A - 纯化重组蛋白的方法 - Google Patents

纯化重组蛋白的方法 Download PDF

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Publication number
CN114885608A
CN114885608A CN202080087052.0A CN202080087052A CN114885608A CN 114885608 A CN114885608 A CN 114885608A CN 202080087052 A CN202080087052 A CN 202080087052A CN 114885608 A CN114885608 A CN 114885608A
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CN
China
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series
chromatography
medium
aminoguanidine
hydrophobic
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Pending
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CN202080087052.0A
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English (en)
Inventor
项炜
岳志蕾
赵洪志
邵聪伟
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Tonghua Anruite Biopharmaceutical Co ltd
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Tonghua Anruite Biopharmaceutical Co ltd
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Publication of CN114885608A publication Critical patent/CN114885608A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormones [GH] (Somatotropin)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/31—Fusion polypeptide fusions, other than Fc, for prolonged plasma life, e.g. albumin

Abstract

本发明提供了一种纯化重组蛋白，特别是重组人白蛋白的方法，包括：(a)将氨基胍和中长链脂肪酸加入含有重组蛋白的样品中；和(b)对所得样品进行层析，所述层析任选用含有氨基胍的层析缓冲液进行。

Description

PCT国内申请，说明书已公开。

Claims (21)

  1. PCT国内申请，权利要求书已公开。
CN202080087052.0A 2020-12-08 2020-12-08 纯化重组蛋白的方法 Pending CN114885608A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2020/134385 WO2022120547A1 (zh) 2020-12-08 2020-12-08 纯化重组蛋白的方法

Publications (1)

Publication Number Publication Date
CN114885608A true CN114885608A (zh) 2022-08-09

Family

ID=81972822

Family Applications (1)

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CN202080087052.0A Pending CN114885608A (zh) 2020-12-08 2020-12-08 纯化重组蛋白的方法

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Country Link
US (1) US20230331772A1 (zh)
EP (1) EP4169938A4 (zh)
JP (1) JP2024501601A (zh)
KR (1) KR20230060524A (zh)
CN (1) CN114885608A (zh)
CA (1) CA3200066A1 (zh)
WO (1) WO2022120547A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115677848A (zh) * 2022-10-24 2023-02-03 通化安睿特生物制药股份有限公司 一种制备高纯度高稳定性蛋白质的方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5986062A (en) * 1992-05-20 1999-11-16 The Green Cross Corporation Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same
CN102234332A (zh) * 2010-04-26 2011-11-09 浙江海正药业股份有限公司 一种重组人血白蛋白及其融合蛋白的分离纯化工艺
CN103880947A (zh) * 2012-12-21 2014-06-25 武汉禾元生物科技有限公司 一种分离纯化高纯度重组人血清白蛋白的层析方法
CN104395338A (zh) * 2012-04-19 2015-03-04 诺和诺德A/S（股份有限公司） 人胰岛淀粉样多肽类似物
CN107033236A (zh) * 2017-05-05 2017-08-11 浙江大学 一种从酵母发酵液中分离人血白蛋白的混合模式层析方法
CN110092827A (zh) * 2019-05-16 2019-08-06 齐智 一种获得高纯度重组人血清白蛋白的纯化方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5440018A (en) * 1992-05-20 1995-08-08 The Green Cross Corporation Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same
US5728553A (en) 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
DE69532492T2 (de) 1994-08-31 2004-12-02 Mitsubishi Pharma Corp. Verfahren zur Reinigung von rekombinantem menschlichem Serumalbumin
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
SE526227C2 (sv) 2002-05-15 2005-08-02 North China Pharmaceutical Group Metod för rening av rekombinant humant serumalbumin
CN1854155B (zh) 2005-04-29 2010-11-17 华北制药集团新药研究开发有限责任公司 一种纯化rHSA的方法
CN101768206B (zh) 2008-12-31 2013-05-15 华北制药集团新药研究开发有限责任公司 一种重组人血清白蛋白的纯化方法及其应用
CN102190722B (zh) 2010-03-16 2014-03-26 上海欣瑞特生物医药技术有限公司 一种纯化重组人血白蛋白的方法
CN102070714B (zh) * 2010-11-26 2013-10-16 大连理工大学 一种重组人血清白蛋白的分离纯化方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5986062A (en) * 1992-05-20 1999-11-16 The Green Cross Corporation Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same
CN102234332A (zh) * 2010-04-26 2011-11-09 浙江海正药业股份有限公司 一种重组人血白蛋白及其融合蛋白的分离纯化工艺
CN104395338A (zh) * 2012-04-19 2015-03-04 诺和诺德A/S（股份有限公司） 人胰岛淀粉样多肽类似物
CN103880947A (zh) * 2012-12-21 2014-06-25 武汉禾元生物科技有限公司 一种分离纯化高纯度重组人血清白蛋白的层析方法
CN107033236A (zh) * 2017-05-05 2017-08-11 浙江大学 一种从酵母发酵液中分离人血白蛋白的混合模式层析方法
CN110092827A (zh) * 2019-05-16 2019-08-06 齐智 一种获得高纯度重组人血清白蛋白的纯化方法

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CA3200066A1 (en) 2022-06-16
WO2022120547A1 (zh) 2022-06-16
EP4169938A1 (en) 2023-04-26
EP4169938A4 (en) 2023-08-09
KR20230060524A (ko) 2023-05-04
JP2024501601A (ja) 2024-01-15
US20230331772A1 (en) 2023-10-19

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