ES2062514T5 - Sistema de procesamiento de levadura que comprende un aminoacido cargado negativamente adyacente al sitio de procesamiento. - Google Patents

Sistema de procesamiento de levadura que comprende un aminoacido cargado negativamente adyacente al sitio de procesamiento.

Info

Publication number
ES2062514T5
ES2062514T5 ES90904258T ES90904258T ES2062514T5 ES 2062514 T5 ES2062514 T5 ES 2062514T5 ES 90904258 T ES90904258 T ES 90904258T ES 90904258 T ES90904258 T ES 90904258T ES 2062514 T5 ES2062514 T5 ES 2062514T5
Authority
ES
Spain
Prior art keywords
amino acid
negatively charged
protein
system including
charged amino
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90904258T
Other languages
English (en)
Other versions
ES2062514T3 (es
Inventor
Soren Bjorn
Kjeld Norris
Fanny Norris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26065137&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2062514(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from DK494189A external-priority patent/DK494189D0/da
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2062514T3 publication Critical patent/ES2062514T3/es
Publication of ES2062514T5 publication Critical patent/ES2062514T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Silver Salt Photography Or Processing Solution Therefor (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Processing Of Solid Wastes (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Particle Accelerators (AREA)
  • Thermotherapy And Cooling Therapy Devices (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

UN POLIPEPTIDO PARA LA PRODUCCION DE LEVADURA COMPRENDE LA FUSION DEL PEPTIDO DE SEÑAL, UN PEPTIDO LIDER Y UNA PROTEINA HETEROLOGUS O UN POLIPEPTIDO. EL POLIPEPTIDO ES MODIFICADO DE SU SECUENCIA AMINOACIDA EN UN LUGAR PROXIMO AL PROCESO, LOCALIZADO EN EL EXTREMO DE LA C-TERMINAL DEL PEPTIDO LIDER Y EN EL EXTREMO N-TERMINAL DE LA PROTEINA HETEROLOGUS, PARA SUMINISTRAR UNA PRESENTACION DEL LUGAR DE PROCESO QUE LO HACE ACCESIBLE A LA RUPTURA PEOTEOLITICA. DICHA PRESENTACION SE SUMINISTRA AÑADIENDO UNO O MAS AMINOACIDOS (AL MENOS UNO QUE ESTA CARGADO NEGATIVAMENTE) A CUALQUIER EXTREMO C-TERMINAL DEL LIDER O AL EXTREMO N-TERMINAL DE LA PROTEINA, O AMBOS. LA PROTEINA HETEROLOGUS PUEDE, POR SUPUESTO, SER UN PRECURSOR OPROTININ O INSULINA O UN ANALOGO DE ELLOS.
ES90904258T 1989-03-03 1990-03-01 Sistema de procesamiento de levadura que comprende un aminoacido cargado negativamente adyacente al sitio de procesamiento. Expired - Lifetime ES2062514T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK105489A DK105489D0 (da) 1989-03-03 1989-03-03 Polypeptid
DK494189A DK494189D0 (da) 1989-10-06 1989-10-06 Polypeptid

Publications (2)

Publication Number Publication Date
ES2062514T3 ES2062514T3 (es) 1994-12-16
ES2062514T5 true ES2062514T5 (es) 1998-06-01

Family

ID=26065137

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90904258T Expired - Lifetime ES2062514T5 (es) 1989-03-03 1990-03-01 Sistema de procesamiento de levadura que comprende un aminoacido cargado negativamente adyacente al sitio de procesamiento.

Country Status (23)

Country Link
US (3) US5395922A (es)
EP (1) EP0461165B2 (es)
JP (1) JP2609367B2 (es)
KR (1) KR970005928B1 (es)
AT (1) ATE110414T1 (es)
AU (1) AU624694B2 (es)
CA (1) CA2050336C (es)
CZ (1) CZ285239B6 (es)
DD (1) DD296965A5 (es)
DE (1) DE69011853T3 (es)
DK (2) DK105489D0 (es)
ES (1) ES2062514T5 (es)
FI (1) FI104985B (es)
HU (2) HU215538B (es)
IE (1) IE66469B1 (es)
IL (1) IL93609A (es)
NO (2) NO304236B1 (es)
NZ (1) NZ232742A (es)
PL (1) PL163532B1 (es)
RU (1) RU2194758C2 (es)
UA (1) UA27706C2 (es)
WO (1) WO1990010075A1 (es)
ZA (1) ZA901476B (es)

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US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
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EP3303380B1 (en) 2015-06-02 2020-01-15 Novo Nordisk A/S Insulins with polar recombinant extensions
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AU1987088A (en) * 1987-06-24 1989-01-19 Novo Nordisk A/S A process for preparing a protein or polypeptide, a dna sequence coding for the polypeptide, a microorganism containing the dna sequence as well as the polypeptide and its use as a pharmaceutical preparation
DK450187D0 (da) * 1987-08-28 1987-08-28 Novo Industri As Fremgangsmaade til fremstilling af proteiner
DK463887D0 (da) * 1987-09-07 1987-09-07 Novo Industri As Gaerleader
JPH01240191A (ja) * 1988-02-16 1989-09-25 Green Cross Corp:The 酵母で機能する新規シグナルペプチドおよびこれを用いた異種蛋白質の分泌発現
DK336188D0 (da) * 1988-06-20 1988-06-20 Nordisk Gentofte Propeptider
JP3092811B2 (ja) * 1988-07-23 2000-09-25 デルタ バイオテクノロジー リミテッド ペプチドおよびｄｎａ配列
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid

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CA2050336C (en) 2000-01-18
HU215538B (hu) 1999-01-28
KR920701452A (ko) 1992-08-11
WO1990010075A1 (en) 1990-09-07
IL93609A0 (en) 1990-12-23
US5514585A (en) 1996-05-07
JP2609367B2 (ja) 1997-05-14
DK0461165T3 (da) 1994-11-21
DE69011853T2 (de) 1994-12-15
ATE110414T1 (de) 1994-09-15
UA27706C2 (uk) 2000-10-16
NO304236B1 (no) 1998-11-16
HU902376D0 (en) 1991-11-28
CZ103990A3 (cs) 1999-03-17
IL93609A (en) 2001-11-25
NO913427D0 (no) 1991-09-02
AU624694B2 (en) 1992-06-18
EP0461165B2 (en) 1998-03-25
DK105489D0 (da) 1989-03-03
US5395922A (en) 1995-03-07
NO974899L (no) 1997-10-23
JPH04504846A (ja) 1992-08-27
EP0461165A1 (en) 1991-12-18
DE69011853D1 (de) 1994-09-29
IE66469B1 (en) 1995-12-27
NO913427L (no) 1991-11-01
AU5261290A (en) 1990-09-26
DD296965A5 (de) 1991-12-19
NZ232742A (en) 1991-10-25
ZA901476B (en) 1990-11-28
PL163532B1 (pl) 1994-04-29
IE900749L (en) 1990-09-03
DE69011853T3 (de) 1998-06-25
KR970005928B1 (ko) 1997-04-22
HU211600A9 (en) 1995-12-28
RU2194758C2 (ru) 2002-12-20
HUT58370A (en) 1992-02-28
FI104985B (fi) 2000-05-15
US5510249A (en) 1996-04-23
ES2062514T3 (es) 1994-12-16
NO974899D0 (no) 1997-10-23
EP0461165B1 (en) 1994-08-24
FI914125A0 (fi) 1991-09-02
CA2050336A1 (en) 1990-09-04
CZ285239B6 (cs) 1999-06-16

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