DE69011853D1 - System zur bearbeitung von hefe, welches eine zur bearbeitungsstelle benachbarte negativ geladene aminosäure beeinhaltet. - Google Patents
System zur bearbeitung von hefe, welches eine zur bearbeitungsstelle benachbarte negativ geladene aminosäure beeinhaltet.Info
- Publication number
- DE69011853D1 DE69011853D1 DE69011853T DE69011853T DE69011853D1 DE 69011853 D1 DE69011853 D1 DE 69011853D1 DE 69011853 T DE69011853 T DE 69011853T DE 69011853 T DE69011853 T DE 69011853T DE 69011853 D1 DE69011853 D1 DE 69011853D1
- Authority
- DE
- Germany
- Prior art keywords
- amino acid
- negatively charged
- yeast
- terminal end
- charged amino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 3
- 150000001413 amino acids Chemical class 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 3
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 210000004899 c-terminal region Anatomy 0.000 abstract 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 108010039627 Aprotinin Proteins 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 229960004405 aprotinin Drugs 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 230000006337 proteolytic cleavage Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Diabetes (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Silver Salt Photography Or Processing Solution Therefor (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Processing Of Solid Wastes (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK105489A DK105489D0 (da) | 1989-03-03 | 1989-03-03 | Polypeptid |
| DK494189A DK494189D0 (da) | 1989-10-06 | 1989-10-06 | Polypeptid |
| PCT/DK1990/000058 WO1990010075A1 (en) | 1989-03-03 | 1990-03-01 | Yeast processing system comprising a negatively charged amino acid adjacent to the processing site |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE69011853D1 true DE69011853D1 (de) | 1994-09-29 |
| DE69011853T2 DE69011853T2 (de) | 1994-12-15 |
| DE69011853T3 DE69011853T3 (de) | 1998-06-25 |
Family
ID=26065137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69011853T Expired - Lifetime DE69011853T3 (de) | 1989-03-03 | 1990-03-01 | System zur bearbeitung von hefe, welches eine zur bearbeitungsstelle benachbarte negativ geladene aminosäure beeinhaltet. |
Country Status (23)
| Country | Link |
|---|---|
| US (3) | US5395922A (de) |
| EP (1) | EP0461165B2 (de) |
| JP (1) | JP2609367B2 (de) |
| KR (1) | KR970005928B1 (de) |
| AT (1) | ATE110414T1 (de) |
| AU (1) | AU624694B2 (de) |
| CA (1) | CA2050336C (de) |
| CZ (1) | CZ285239B6 (de) |
| DD (1) | DD296965A5 (de) |
| DE (1) | DE69011853T3 (de) |
| DK (2) | DK105489D0 (de) |
| ES (1) | ES2062514T5 (de) |
| FI (1) | FI104985B (de) |
| HU (2) | HU215538B (de) |
| IE (1) | IE66469B1 (de) |
| IL (1) | IL93609A (de) |
| NO (2) | NO304236B1 (de) |
| NZ (1) | NZ232742A (de) |
| PL (1) | PL163532B1 (de) |
| RU (1) | RU2194758C2 (de) |
| UA (1) | UA27706C2 (de) |
| WO (1) | WO1990010075A1 (de) |
| ZA (1) | ZA901476B (de) |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5591603A (en) * | 1987-08-28 | 1997-01-07 | Novo Nordisk A/S | Process for preparing aprotinin and aprotinin analogs in yeast cells |
| DK105489D0 (da) * | 1989-03-03 | 1989-03-03 | Novo Nordisk As | Polypeptid |
| EP0472658A4 (en) * | 1989-05-19 | 1992-08-26 | Biotechnology Res & Dev | Fusion proteins having an in vivo post-translational modification site and methods of manufacture and purification |
| GB9015825D0 (en) * | 1990-07-18 | 1990-09-05 | Ciba Geigy Ag | In vitro processing of fusion proteins |
| DK300090D0 (da) * | 1990-12-19 | 1990-12-19 | Novo Nordisk As | Fremgangsmaade til fremstilling af leadersekvenser |
| GB9106185D0 (en) * | 1991-03-22 | 1991-05-08 | Wellcome Found | Biological control agents |
| FR2686899B1 (fr) * | 1992-01-31 | 1995-09-01 | Rhone Poulenc Rorer Sa | Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant. |
| FI92601C (fi) * | 1992-03-11 | 1994-12-12 | Marja Makarow | Menetelmä hyötyproteiinien erittämiseksi hiivoista |
| US5521086A (en) * | 1993-09-16 | 1996-05-28 | Cephalon, Inc. | Secretion sequence for the production of a heterologous protein in yeast |
| DE4417353A1 (de) * | 1994-05-18 | 1996-01-25 | Bayer Ag | Verfahren zur Herstellung von rekombinantem Aprotinin und rekombinanten Aprotinin Varianten mit der natürlichen N-terminlaen Sequenz |
| IL114160A (en) * | 1994-06-17 | 2006-12-31 | Novo Nordisk As | Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast |
| US6500645B1 (en) | 1994-06-17 | 2002-12-31 | Novo Nordisk A/S | N-terminally extended proteins expressed in yeast |
| US5861267A (en) * | 1995-05-01 | 1999-01-19 | Vertex Pharmaceuticals Incorporated | Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity |
| ZA9610456B (en) * | 1995-12-20 | 1997-06-20 | Novo Nordisk As | N-terminally extended proteins expressed in yeast |
| US5851800A (en) * | 1996-05-14 | 1998-12-22 | Pharmacia & Upjohn Ab | Process for producing a protein |
| DE19629982A1 (de) | 1996-07-25 | 1998-01-29 | Bayer Ag | Aprotinin-Varianten mit verbesserten Eigenschaften |
| EP0948538B1 (de) * | 1996-12-13 | 2008-06-11 | Novartis Vaccines and Diagnostics, Inc. | Analyse und separation von pdgf-proteinen |
| DE69735242T2 (de) * | 1996-12-20 | 2006-09-21 | Novo Nordisk A/S | N-terminal verlängerte proteine exprimiert in hefe |
| JP2001527387A (ja) * | 1997-01-24 | 2001-12-25 | ノボ ノルディスク アクティーゼルスカブ | 合成リーダーペプチド配列 |
| US6046000A (en) * | 1997-11-07 | 2000-04-04 | Millennium Biotherapeutics, Inc. | Method for identifying genes encoding signal sequences |
| WO1999037793A1 (en) | 1998-01-23 | 1999-07-29 | Novo Nordisk A/S | Process for making desired polypeptides in yeast |
| ATE474930T1 (de) | 1999-12-29 | 2010-08-15 | Novo Nordisk As | Verfahren zur herstellung von insulinvorläufern und analogen von insulinvorläufern mit verbesserter fermentationsausbeute in hefe |
| AU2001266557A1 (en) * | 2000-04-12 | 2001-10-23 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| US20050100991A1 (en) * | 2001-04-12 | 2005-05-12 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| US20050054051A1 (en) * | 2001-04-12 | 2005-03-10 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| US7507413B2 (en) * | 2001-04-12 | 2009-03-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| US7595172B2 (en) | 2001-07-24 | 2009-09-29 | Novo Nordisk A/S | Method for making acylated polypeptides |
| WO2003059934A2 (en) * | 2001-12-21 | 2003-07-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| EP2261250B1 (de) | 2001-12-21 | 2015-07-01 | Human Genome Sciences, Inc. | GCSF-Albumin Fusionsproteine |
| US7309505B2 (en) | 2002-09-13 | 2007-12-18 | Cornell Research Foundation, Inc. | Using mutations to improve Aspergillus phytases |
| CA2513213C (en) | 2003-01-22 | 2013-07-30 | Human Genome Sciences, Inc. | Albumin fusion proteins |
| EP1687428A1 (de) | 2003-11-14 | 2006-08-09 | Novo Nordisk A/S | Verfahren zur herstellung von acyliertem insulin |
| JP5697831B2 (ja) | 2003-12-03 | 2015-04-08 | ノヴォ ノルディスク アー／エス | 単鎖インシュリン |
| EP1917363B1 (de) | 2005-08-16 | 2011-06-22 | Novo Nordisk A/S | Verfahren zur herstellung reifer insulinpolypeptide |
| JP5202338B2 (ja) | 2006-02-27 | 2013-06-05 | ノボ・ノルデイスク・エー／エス | 新規インシュリン誘導体 |
| US8198046B2 (en) | 2006-07-11 | 2012-06-12 | Danisco Us Inc. | KEX2 cleavage regions of recombinant fusion proteins |
| US20080026376A1 (en) * | 2006-07-11 | 2008-01-31 | Huaming Wang | KEX2 cleavage regions of recombinant fusion proteins |
| EP2404934A1 (de) | 2006-09-22 | 2012-01-11 | Novo Nordisk A/S | Proteaseresistente Insulinanaloga |
| ES2464282T3 (es) | 2006-09-27 | 2014-06-02 | Novo Nordisk A/S | Método para preparar polipéptidos de insulina madura |
| JP5404411B2 (ja) | 2006-11-22 | 2014-01-29 | ノボ・ノルデイスク・エー／エス | 活性化カルボキシペプチダーゼの製造方法 |
| EP2170945A1 (de) | 2007-07-16 | 2010-04-07 | Novo Nordisk A/S | Proteasestabilisierte pegylierte insulinanaloga |
| JP5710253B2 (ja) | 2007-08-13 | 2015-04-30 | ノボ・ノルデイスク・エー／エス | 速効型インスリンアナログ |
| ES2548304T3 (es) | 2007-08-15 | 2015-10-15 | Novo Nordisk A/S | Análogos de la insulina que contienen una fracción acilo y alquilenglicol |
| JP5721432B2 (ja) | 2007-08-15 | 2015-05-20 | ノボ・ノルデイスク・エー／エス | アミノ酸含有アルキレングリコール反復単位を含むアシル部を有するインスリン |
| US8501449B2 (en) | 2007-12-04 | 2013-08-06 | Proteon Therapeutics, Inc. | Recombinant elastase proteins and methods of manufacturing and use thereof |
| AU2009226910B2 (en) | 2008-03-18 | 2014-02-06 | Novo Nordisk A/S | Protease stabilized, acylated insulin analogues |
| EP2262539B1 (de) | 2008-04-01 | 2015-07-15 | Novo Nordisk A/S | Insulin-albumin-konzentrate |
| WO2010103546A2 (en) * | 2009-03-12 | 2010-09-16 | Bigtec Private Limited | A polynucleotide and polypeptide sequence and methods thereof |
| HUE027239T2 (en) | 2009-06-26 | 2016-10-28 | Novo Nordisk As | Preparation containing insulin, nicotinamide and arginine |
| EP2504355B1 (de) | 2009-11-25 | 2015-07-22 | Novo Nordisk A/S | Verfahren zur herstellung von polypeptiden |
| EP2526117B1 (de) | 2010-01-22 | 2015-05-06 | Novo Nordisk A/S | Verfahren zur herstellung von fgf-21 mit geringer o-glykosylierung |
| US8853155B2 (en) | 2010-06-23 | 2014-10-07 | Novo Nordisk A/S | Insulin derivatives containing additional disulfide bonds |
| JP6013330B2 (ja) | 2010-06-23 | 2016-10-25 | ノヴォ ノルディスク アー／エス | 追加のジスルフィド結合を含有するヒトインスリン |
| CN102947331B (zh) | 2010-06-23 | 2016-08-03 | 诺沃—诺迪斯克有限公司 | 包含额外的二硫键的胰岛素类似物 |
| MX2013006174A (es) | 2010-12-14 | 2013-07-15 | Novo Nordisk As | Preparacion que comprende insulina, nicotinamida y un aminoacido. |
| JP2013545782A (ja) | 2010-12-14 | 2013-12-26 | ノヴォ ノルディスク アー／エス | 長時間作用型インスリンと組み合わせた速効型インスリン |
| WO2012171994A1 (en) | 2011-06-15 | 2012-12-20 | Novo Nordisk A/S | Multi substituted insulins |
| RU2460795C1 (ru) * | 2011-07-06 | 2012-09-10 | Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") | Способ микробиологического синтеза секретируемого соматотропина человека и штамм дрожжей saccharomyces cerevisiae - продуцент секретируемого соматотропина человека |
| WO2013186138A1 (en) | 2012-06-14 | 2013-12-19 | Novo Nordisk A/S | Preparation comprising insulin, nicotinamide and arginine |
| US9707276B2 (en) | 2012-12-03 | 2017-07-18 | Merck Sharp & Dohme Corp. | O-glycosylated carboxy terminal portion (CTP) peptide-based insulin and insulin analogues |
| CN103254277B (zh) * | 2013-05-03 | 2017-02-08 | 南京工业大学 | 强分泌性信号肽增强小肽模序及其应用 |
| JP2016521701A (ja) | 2013-06-07 | 2016-07-25 | ノヴォ ノルディスク アー／エス | 成熟インスリンポリペプチドを作製するための方法 |
| PL233560B1 (pl) | 2014-12-05 | 2019-10-31 | Mabion Spolka Akcyjna | Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka |
| WO2016144658A1 (en) | 2015-03-10 | 2016-09-15 | Merck Sharp & Dohme Corp. | Process for preparing recombinant insulin using microfiltration |
| JP6983075B2 (ja) | 2015-06-02 | 2021-12-17 | ノヴォ ノルディスク アー／エス | 極性の組換え延長部を有するインスリン |
| US20180244743A1 (en) | 2015-08-25 | 2018-08-30 | Novo Nordisk A/S | Novel Insulin Derivatives and the Medical Uses Hereof |
| CN108026156A (zh) | 2015-08-25 | 2018-05-11 | 诺和诺德股份有限公司 | 新型胰岛素衍生物及其医学用途 |
| AR106364A1 (es) | 2015-08-25 | 2018-01-10 | Novo Nordisk As | Derivados de insulina y sus usos médicos |
| KR102043067B1 (ko) * | 2017-08-08 | 2019-11-11 | 엘지전자 주식회사 | 차량용 사이드 미러 및 차량 |
| WO2019034726A1 (en) | 2017-08-17 | 2019-02-21 | Novo Nordisk A/S | NOVEL ACYLATED INSULIN ANALOGUES AND USES THEREOF |
| US11498951B2 (en) | 2019-12-11 | 2022-11-15 | Novo Nordisk A/S | Insulin analogues and uses thereof |
| WO2023144240A1 (en) | 2022-01-26 | 2023-08-03 | Novo Nordisk Research Centre Oxford Limited | Glucose sensitive insulin derivatives and uses thereof |
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| AU5712580A (en) * | 1979-04-05 | 1980-10-09 | James Joseph Connell | Engine charging and scavenging |
| US4546082A (en) * | 1982-06-17 | 1985-10-08 | Regents Of The Univ. Of California | E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins |
| JPS60501140A (ja) * | 1983-04-22 | 1985-07-25 | アムジエン | 酵母による外因性ポリペプチドの分泌 |
| US4588684A (en) * | 1983-04-26 | 1986-05-13 | Chiron Corporation | a-Factor and its processing signals |
| DK58285D0 (da) * | 1984-05-30 | 1985-02-08 | Novo Industri As | Peptider samt fremstilling og anvendelse deraf |
| EP0195680A3 (de) * | 1985-03-21 | 1987-06-10 | Immunex Corporation | Proteinsynthese mit einem Identifizierungspeptid |
| JPS6236183A (ja) * | 1985-06-20 | 1987-02-17 | ザ・サルク・インステイチユ−ト・バイオテクノロジ−／インダストリアル・アソシエイツ・インコ−ポレ−テツド | サツカロミセス・セレビシエからのポリペプチドの発現および分泌 |
| MC1834A1 (fr) * | 1986-07-10 | 1988-06-03 | Transgene Sa | Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation |
| DK175535B1 (da) * | 1987-03-04 | 2004-11-29 | Daiichi Suntory Pharma Co Ltd | Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid |
| EP0371041A1 (de) * | 1987-06-24 | 1990-06-06 | Novo Nordisk A/S | Verfahren für die herstellung eines proteins oder polypeptides, dns-sequenz, die dieses polypeptid kodiert, mikroorganismus, der diese dns-sequenz beinhaltet, sowie das polypeptid und seine verwendung als pharmazeutisches präparat |
| DK450187D0 (da) * | 1987-08-28 | 1987-08-28 | Novo Industri As | Fremgangsmaade til fremstilling af proteiner |
| DK463887D0 (da) * | 1987-09-07 | 1987-09-07 | Novo Industri As | Gaerleader |
| JPH01240191A (ja) * | 1988-02-16 | 1989-09-25 | Green Cross Corp:The | 酵母で機能する新規シグナルペプチドおよびこれを用いた異種蛋白質の分泌発現 |
| DK336188D0 (da) * | 1988-06-20 | 1988-06-20 | Nordisk Gentofte | Propeptider |
| EP0387319B1 (de) * | 1988-07-23 | 1996-03-06 | Delta Biotechnology Limited | Sekretorische leader-sequenzen |
| DK105489D0 (da) * | 1989-03-03 | 1989-03-03 | Novo Nordisk As | Polypeptid |
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1989
- 1989-03-03 DK DK105489A patent/DK105489D0/da not_active Application Discontinuation
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1990
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- 1990-03-01 RU SU5010019/13A patent/RU2194758C2/ru not_active IP Right Cessation
- 1990-03-01 AU AU52612/90A patent/AU624694B2/en not_active Ceased
- 1990-03-01 DK DK90904258.2T patent/DK0461165T3/da active
- 1990-03-01 KR KR1019910701029A patent/KR970005928B1/ko not_active IP Right Cessation
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- 1990-03-01 WO PCT/DK1990/000058 patent/WO1990010075A1/en active IP Right Grant
- 1990-03-01 DE DE69011853T patent/DE69011853T3/de not_active Expired - Lifetime
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1991
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1994
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