DE69432185D1 - Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe - Google Patents

Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe

Info

Publication number
DE69432185D1
DE69432185D1 DE69432185T DE69432185T DE69432185D1 DE 69432185 D1 DE69432185 D1 DE 69432185D1 DE 69432185 T DE69432185 T DE 69432185T DE 69432185 T DE69432185 T DE 69432185T DE 69432185 D1 DE69432185 D1 DE 69432185D1
Authority
DE
Germany
Prior art keywords
yeast
sequence encoding
dna molecule
protein
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69432185T
Other languages
English (en)
Other versions
DE69432185T2 (de
Inventor
Richard W Scott
Russell Arthur Brierley
David Scott Howland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cephalon LLC
Original Assignee
Cephalon LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cephalon LLC filed Critical Cephalon LLC
Application granted granted Critical
Publication of DE69432185D1 publication Critical patent/DE69432185D1/de
Publication of DE69432185T2 publication Critical patent/DE69432185T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69432185T 1993-09-16 1994-08-24 Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe Expired - Lifetime DE69432185T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US122889 1993-09-16
US08/122,889 US5521086A (en) 1993-09-16 1993-09-16 Secretion sequence for the production of a heterologous protein in yeast
PCT/US1994/009653 WO1995007978A1 (en) 1993-09-16 1994-08-24 A secretion sequence for the production of a heterologous protein in yeast

Publications (2)

Publication Number Publication Date
DE69432185D1 true DE69432185D1 (de) 2003-04-03
DE69432185T2 DE69432185T2 (de) 2004-02-05

Family

ID=22405437

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69432185T Expired - Lifetime DE69432185T2 (de) 1993-09-16 1994-08-24 Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe

Country Status (9)

Country Link
US (1) US5521086A (de)
EP (1) EP0728191B1 (de)
JP (1) JPH09502609A (de)
AT (1) ATE233314T1 (de)
AU (1) AU689496B2 (de)
DE (1) DE69432185T2 (de)
FI (1) FI961238A (de)
NZ (1) NZ271928A (de)
WO (1) WO1995007978A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ207842A (en) * 1983-04-25 1988-02-12 Chiron Corp Production of human insulin-like growth factor (igf) using cdna
FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
JPH10504971A (ja) * 1994-09-08 1998-05-19 カイロン コーポレイション インスリン様増殖因子の改善された生産方法
EP1003889A1 (de) 1997-08-05 2000-05-31 Chiron Corporation Gensequenzen aus pichia pastoris und deren verwendung
US7351549B2 (en) * 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
CA2405557C (en) * 2000-04-12 2013-09-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US6605450B1 (en) 2000-05-25 2003-08-12 Chiron Corporation Iron regulation of the PpSEC10 transcriptional regulatory region of Pichia pastoris and methods of use
US7507413B2 (en) * 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20050054051A1 (en) * 2001-04-12 2005-03-10 Human Genome Sciences, Inc. Albumin fusion proteins
ES2545090T3 (es) * 2001-12-21 2015-09-08 Human Genome Sciences, Inc. Proteínas de fusión de albúmina y GCSF
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
WO2003059934A2 (en) * 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
JP2011507507A (ja) * 2007-12-21 2011-03-10 フジフィルム・マニュファクチュアリング・ヨーロッパ・ベスローテン・フエンノートシャップ マルチマー性前駆体のｉｎｖｉｖｏタンパク質分解的プロセシングによる関心対象のタンパク質の分泌収量の改善
US9023798B2 (en) 2009-07-24 2015-05-05 The Regents Of The University Of Michigan Cystinosin replacement factor
CA2974539A1 (en) 2015-02-06 2016-08-11 Cargill, Incorporated Modified glucoamylase enzymes and yeast strains having enhanced bioproduct production
BR112017020474A2 (pt) 2015-03-27 2018-07-03 Cargill Inc levedura modificada, meio de fermentação, método para produção de um bioproduto, ácido nucleico, vetor e método de fermentação para produzir etanol
AU2017259656B2 (en) * 2016-05-04 2023-01-12 Shenzhen Protgen Ltd. Construction of engineering bacteria for high expression of recombinant human serum albumin
WO2018027131A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
NZ207842A (en) * 1983-04-25 1988-02-12 Chiron Corp Production of human insulin-like growth factor (igf) using cdna
US4870008A (en) * 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
EP0188555A4 (de) * 1984-07-09 1988-04-18 Integrated Genetics Inc Klonierungsvektor für hefe.
US4963665A (en) * 1986-01-07 1990-10-16 Washington University Human preproinsulin-like growth factor I
US5070075A (en) * 1986-01-07 1991-12-03 Washington University Human preproinsulin-like growth factor I
KR100195632B1 (ko) * 1988-07-23 1999-06-15 스티븐 조지 가랜드 펩티드 및 dna 배열
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
JPH06500470A (ja) * 1990-09-04 1994-01-20 メルク　エンド　カンパニー　インコーポレーテッド メチロトローフ酵母細胞におけるインスリン様成長因子の産生
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same

Also Published As

Publication number Publication date
EP0728191A1 (de) 1996-08-28
NZ271928A (en) 1997-10-24
DE69432185T2 (de) 2004-02-05
WO1995007978A1 (en) 1995-03-23
US5521086A (en) 1996-05-28
EP0728191B1 (de) 2003-02-26
FI961238A0 (fi) 1996-03-15
AU689496B2 (en) 1998-04-02
JPH09502609A (ja) 1997-03-18
FI961238A (fi) 1996-05-10
AU7604794A (en) 1995-04-03
ATE233314T1 (de) 2003-03-15
EP0728191A4 (de) 1999-07-14

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